P09187 (LGB1_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Leghemoglobin-1 Alternative name(s): Leghemoglobin I
Organism	Medicago sativa (Alfalfa)
Taxonomic identifier	3879 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Provides oxygen to the bacteroids. This role is essential for symbiotic nitrogen fixation.
Subunit structure	Monomer.
Tissue specificity	Root nodules.
Sequence similarities	Belongs to the plant globin family.

Ontologies

Keywords
Biological process	Nitrogen fixation Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Leghemoglobin-1		PRO_0000192986
Sites
	Metal binding	62	1	Iron (heme distal ligand) By similarity
	Metal binding	94	1	Iron (heme proximal ligand) By similarity
Experimental info
	Sequence conflict	33	1	V → I in CAA32492. Ref.2
	Sequence conflict	33	1	V → I in AAA32657. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P09187 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: DBD0A94E743CB28A	FASTA	147	16,055
	10 20 30 40 50 60 MSFTDKQEAL VNSSWEAFKQ NLPRYSVFFY TVVLEKAPAA KGLFSFLKNS AEVQDSPQLQ 70 80 90 100 110 120 AHAEKVFGLV RDSAVQLRAT GGVVLGDATL GAIHVRKGVV DPHFVVVKEA LLKTIKEAAG 130 140 DKWSEELNTA WEVAYDALAT AIKKAMS

References

[1]	"Nucleotide sequence of a cDNA clone encoding a leghemoglobin from Medicago sativa." Davidowitz E.J., Lang-Unnasch N. Nucleic Acids Res. 17:3307-3307(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Iroquois.
[2]	"Identification of two groups of leghemoglobin genes in alfalfa (Medicago sativa) and a study of their expression during root nodule development." Barker D.G., Gallusci P., Lullien V., Khan H., Gherardi M., Huguet T. Plant Mol. Biol. 11:761-772(1988) [AGRICOLA] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Hybrid 11 X 8. Tissue: Root nodule.
[3]	"Sequence analysis of alfalfa (Medicago sativa) leghemoglobin cDNA and genomic clones." Davidowitz E.J., Creissen G., Vincze E., Kiss G.B., Lang-Unnasch N. Plant Mol. Biol. 16:161-165(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13375 mRNA. Translation: CAA31750.1. X14311 mRNA. Translation: CAA32492.1. M36100 mRNA. Translation: AAA32657.1.
PIR	S08508. S15768.
3D structure databases
ProteinModelPortal	P09187.
SMR	P09187. Positions 1-145.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.490.10. 1 hit.
InterPro	IPR000971. Globin. IPR009050. Globin-like. IPR012292. Globin_dom. IPR001032. Leghaemoglobin. IPR019824. Leghaemoglobin_Fe_BS. [Graphical view]
Pfam	PF00042. Globin. 1 hit. [Graphical view]
PRINTS	PR00188. PLANTGLOBIN.
SUPFAM	SSF46458. Globin_like. 1 hit.
PROSITE	PS01033. GLOBIN. 1 hit. PS00208. PLANT_GLOBIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGB1_MEDSA

Accession

Primary (citable) accession number: P09187

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program