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Protein

Seed linoleate 9S-lipoxygenase-3

Gene

LOX1.3

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Catalytic activityi

Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi518Iron; catalytic1
Metal bindingi523Iron; catalytic1
Metal bindingi709Iron; catalytic1
Metal bindingi713Iron; catalytic1
Metal bindingi857Iron; via carboxylate; catalytic1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.B6. 2483.
UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Seed linoleate 9S-lipoxygenase-3 (EC:1.13.11.58)
Alternative name(s):
Lipoxygenase-3
Short name:
L-3
Gene namesi
Name:LOX1.3
Synonyms:LOX3
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi713N → A or S: No loss of iron-binding; loss of catalytic activity. 1 Publication1
Mutagenesisi713N → H: No loss of iron-binding; no change in catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002207191 – 857Seed linoleate 9S-lipoxygenase-3Add BLAST857

Proteomic databases

PRIDEiP09186.

Interactioni

Subunit structurei

Monomer.5 Publications

Protein-protein interaction databases

STRINGi3847.GLYMA15G03030.1.

Structurei

Secondary structure

1857
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 20Combined sources11
Helixi21 – 23Combined sources3
Turni26 – 30Combined sources5
Turni39 – 41Combined sources3
Beta strandi44 – 48Combined sources5
Helixi50 – 53Combined sources4
Beta strandi55 – 68Combined sources14
Beta strandi70 – 76Combined sources7
Beta strandi84 – 86Combined sources3
Turni89 – 91Combined sources3
Beta strandi97 – 100Combined sources4
Beta strandi103 – 105Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi112 – 119Combined sources8
Beta strandi121 – 123Combined sources3
Beta strandi125 – 133Combined sources9
Beta strandi135 – 137Combined sources3
Beta strandi141 – 149Combined sources9
Helixi152 – 154Combined sources3
Beta strandi155 – 157Combined sources3
Beta strandi159 – 162Combined sources4
Turni169 – 171Combined sources3
Turni174 – 176Combined sources3
Helixi177 – 188Combined sources12
Helixi213 – 215Combined sources3
Helixi217 – 219Combined sources3
Beta strandi224 – 230Combined sources7
Beta strandi246 – 250Combined sources5
Helixi260 – 262Combined sources3
Helixi269 – 271Combined sources3
Helixi273 – 278Combined sources6
Turni279 – 283Combined sources5
Helixi284 – 293Combined sources10
Beta strandi294 – 297Combined sources4
Helixi304 – 308Combined sources5
Turni309 – 313Combined sources5
Helixi319 – 324Combined sources6
Turni325 – 327Combined sources3
Beta strandi328 – 330Combined sources3
Beta strandi332 – 335Combined sources4
Beta strandi339 – 341Combined sources3
Helixi349 – 351Combined sources3
Helixi357 – 359Combined sources3
Helixi361 – 370Combined sources10
Beta strandi371 – 373Combined sources3
Beta strandi382 – 384Combined sources3
Beta strandi390 – 392Combined sources3
Helixi393 – 396Combined sources4
Helixi403 – 405Combined sources3
Beta strandi408 – 410Combined sources3
Helixi415 – 420Combined sources6
Beta strandi424 – 428Combined sources5
Turni431 – 433Combined sources3
Helixi434 – 436Combined sources3
Helixi437 – 441Combined sources5
Turni442 – 444Combined sources3
Beta strandi449 – 456Combined sources8
Beta strandi462 – 472Combined sources11
Beta strandi474 – 477Combined sources4
Beta strandi482 – 486Combined sources5
Helixi492 – 515Combined sources24
Helixi516 – 522Combined sources7
Helixi523 – 536Combined sources14
Helixi542 – 547Combined sources6
Helixi548 – 551Combined sources4
Helixi554 – 564Combined sources11
Beta strandi566 – 569Combined sources4
Helixi571 – 575Combined sources5
Helixi579 – 581Combined sources3
Helixi582 – 589Combined sources8
Helixi590 – 592Combined sources3
Helixi595 – 598Combined sources4
Helixi600 – 606Combined sources7
Beta strandi609 – 612Combined sources4
Beta strandi619 – 625Combined sources7
Helixi628 – 651Combined sources24
Helixi656 – 659Combined sources4
Helixi662 – 673Combined sources12
Turni674 – 676Combined sources3
Helixi677 – 679Combined sources3
Beta strandi688 – 690Combined sources3
Helixi691 – 705Combined sources15
Helixi707 – 713Combined sources7
Helixi716 – 720Combined sources5
Turni723 – 725Combined sources3
Beta strandi730 – 732Combined sources3
Beta strandi737 – 739Combined sources3
Helixi740 – 747Combined sources8
Helixi749 – 754Combined sources6
Helixi760 – 773Combined sources14
Turni783 – 785Combined sources3
Helixi795 – 818Combined sources24
Helixi825 – 827Combined sources3
Turni828 – 832Combined sources5
Beta strandi844 – 846Combined sources3
Beta strandi852 – 854Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HU9X-ray2.20A1-857[»]
1IK3X-ray2.00A1-857[»]
1JNQX-ray2.10A1-857[»]
1LNHX-ray2.60A1-857[»]
1N8QX-ray2.10A1-857[»]
1NO3X-ray2.15A1-857[»]
1ROVX-ray2.00A1-857[»]
1RRHX-ray2.00A1-857[»]
1RRLX-ray2.09A/B1-857[»]
ProteinModelPortaliP09186.
SMRiP09186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09186.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 163PLATPROSITE-ProRule annotationAdd BLAST126
Domaini166 – 857LipoxygenasePROSITE-ProRule annotationAdd BLAST692

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410XT0Q. LUCA.
InParanoidiP09186.
KOiK15718.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGGLLHRGH KIKGTVVLMR KNVLHVNSVT SVGGIIGQGL DLVGSTLDTL
60 70 80 90 100
TAFLGRPVSL QLISATKADA NGKGKLGKAT FLEGIITSLP TLGAGQSAFK
110 120 130 140 150
INFEWDDGSG ILGAFYIKNF MQTEFFLVSL TLEDIPNHGS IHFVCNSWIY
160 170 180 190 200
NAKLFKSDRI FFANQTYLPS ETPAPLVKYR EEELHNLRGD GTGERKEWER
210 220 230 240 250
VYDYDVYNDL GDPDKGENHA RPVLGGNDTF PYPRRGRTGR KPTRKDPNSE
260 270 280 290 300
SRSNDVYLPR DEAFGHLKSS DFLTYGLKSV SQNVLPLLQS AFDLNFTPRE
310 320 330 340 350
FDSFDEVHGL YSGGIKLPTD IISKISPLPV LKEIFRTDGE QALKFPPPKV
360 370 380 390 400
IQVSKSAWMT DEEFAREMLA GVNPNLIRCL KEFPPRSKLD SQVYGDHTSQ
410 420 430 440 450
ITKEHLEPNL EGLTVDEAIQ NKRLFLLGHH DPIMPYLRRI NATSTKAYAT
460 470 480 490 500
RTILFLKNDG TLRPLAIELS LPHPQGDQSG AFSQVFLPAD EGVESSIWLL
510 520 530 540 550
AKAYVVVNDS CYHQLVSHWL NTHAVVEPFI IATNRHLSVV HPIYKLLHPH
560 570 580 590 600
YRDTMNINGL ARLSLVNDGG VIEQTFLWGR YSVEMSAVVY KDWVFTDQAL
610 620 630 640 650
PADLIKRGMA IEDPSCPHGI RLVIEDYPYA VDGLEIWDAI KTWVHEYVFL
660 670 680 690 700
YYKSDDTLRE DPELQACWKE LVEVGHGDKK NEPWWPKMQT REELVEACAI
710 720 730 740 750
IIWTASALHA AVNFGQYPYG GLILNRPTLS RRFMPEKGSA EYEELRKNPQ
760 770 780 790 800
KAYLKTITPK FQTLIDLSVI EILSRHASDE VYLGERDNPN WTSDTRALEA
810 820 830 840 850
FKRFGNKLAQ IENKLSERNN DEKLRNRCGP VQMPYTLLLP SSKEGLTFRG

IPNSISI
Length:857
Mass (Da):96,758
Last modified:July 1, 1989 - v1
Checksum:i5DFC33D0F6FD32F0
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti25H → D in strain: cv. Provar. 1
Natural varianti57P → S in strain: cv. Provar. 1
Natural varianti112L → P in strain: cv. Provar. 1
Natural varianti201V → I in strain: cv. Provar. 1
Natural varianti382E → D in strain: cv. Provar. 1
Natural varianti428G → D in strain: cv. Provar. 1
Natural varianti630A → T in strain: cv. Provar. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06928 Genomic DNA. Translation: CAA30016.1.
X13302 mRNA. Translation: CAA31664.1. Sequence problems.
U50081 mRNA. Translation: AAB41272.1.
PIRiS01864.
RefSeqiNP_001235383.1. NM_001248454.1.
UniGeneiGma.30739.

Genome annotation databases

GeneIDi547869.
KEGGigmx:547869.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06928 Genomic DNA. Translation: CAA30016.1.
X13302 mRNA. Translation: CAA31664.1. Sequence problems.
U50081 mRNA. Translation: AAB41272.1.
PIRiS01864.
RefSeqiNP_001235383.1. NM_001248454.1.
UniGeneiGma.30739.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HU9X-ray2.20A1-857[»]
1IK3X-ray2.00A1-857[»]
1JNQX-ray2.10A1-857[»]
1LNHX-ray2.60A1-857[»]
1N8QX-ray2.10A1-857[»]
1NO3X-ray2.15A1-857[»]
1ROVX-ray2.00A1-857[»]
1RRHX-ray2.00A1-857[»]
1RRLX-ray2.09A/B1-857[»]
ProteinModelPortaliP09186.
SMRiP09186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3847.GLYMA15G03030.1.

Proteomic databases

PRIDEiP09186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi547869.
KEGGigmx:547869.

Phylogenomic databases

eggNOGiENOG410XT0Q. LUCA.
InParanoidiP09186.
KOiK15718.

Enzyme and pathway databases

UniPathwayiUPA00382.
BRENDAi1.13.11.B6. 2483.

Miscellaneous databases

EvolutionaryTraceiP09186.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOX3_SOYBN
AccessioniPrimary (citable) accession number: P09186
Secondary accession number(s): Q39838
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Soybean contains at least 4 distinct isoenzymes, L-1, L-2, L-3a and L-3b in dry seeds, and at least two distinct isozymes in the hypocotyl/radicle region of the seedling stem.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.