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Protein

Mucorpepsin

Gene
N/A
Organism
Rhizomucor pusillus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme, capable of clotting milk is frequently used for cheese production.

Catalytic activityi

Hydrolysis of proteins, favoring hydrophobic residues at P1 and P1'. Clots milk. Does not accept Lys at P1, and hence does not activate trypsinogen.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041
Active sitei303 – 3031

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Mucorpepsin (EC:3.4.23.23)
Alternative name(s):
Mucor rennin
OrganismiRhizomucor pusillus
Taxonomic identifieri4840 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotinaMucoralesLichtheimiaceaeRhizomucor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Add
BLAST
Propeptidei23 – 6644Activation peptide1 PublicationPRO_0000025895Add
BLAST
Chaini67 – 427361MucorpepsinPRO_0000025896Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi117 ↔ 123
Glycosylationi254 – 2541N-linked (GlcNAc...)
Disulfide bondi338 ↔ 382

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Structurei

Secondary structure

1
427
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi73 – 808Combined sources
Turni81 – 844Combined sources
Beta strandi85 – 928Combined sources
Turni93 – 964Combined sources
Beta strandi97 – 1048Combined sources
Beta strandi111 – 1144Combined sources
Helixi119 – 1213Combined sources
Helixi131 – 1333Combined sources
Beta strandi138 – 14710Combined sources
Beta strandi152 – 16413Combined sources
Beta strandi167 – 18216Combined sources
Helixi183 – 1853Combined sources
Beta strandi197 – 2004Combined sources
Helixi204 – 2063Combined sources
Helixi208 – 2136Combined sources
Helixi220 – 2267Combined sources
Beta strandi229 – 23810Combined sources
Beta strandi242 – 25211Combined sources
Helixi255 – 2573Combined sources
Beta strandi258 – 2603Combined sources
Beta strandi263 – 2664Combined sources
Beta strandi268 – 2703Combined sources
Beta strandi273 – 28614Combined sources
Beta strandi289 – 30315Combined sources
Beta strandi308 – 3125Combined sources
Helixi313 – 32311Combined sources
Beta strandi328 – 3303Combined sources
Beta strandi333 – 3375Combined sources
Helixi338 – 3414Combined sources
Beta strandi347 – 3537Combined sources
Beta strandi361 – 3677Combined sources
Helixi368 – 3714Combined sources
Beta strandi372 – 3743Combined sources
Beta strandi376 – 3794Combined sources
Beta strandi381 – 39212Combined sources
Beta strandi395 – 3973Combined sources
Helixi398 – 4014Combined sources
Beta strandi404 – 4096Combined sources
Turni410 – 4134Combined sources
Beta strandi414 – 4207Combined sources
Turni422 – 4243Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MPPX-ray2.00A67-426[»]
ProteinModelPortaliP09177.
SMRiP09177. Positions 71-427.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09177.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 418333Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFSKISSAI LLTAASFALT SARPVSKQSD ADDKLLALPL TSVNRKYSQT
60 70 80 90 100
KHGQQAAEKL GGIKAFAEGD GSVDTPGLYD FDLEEYAIPV SIGTPGQDFY
110 120 130 140 150
LLFDTGSSDT WVPHKGCDNS EGCVGKRFFD PSSSSTFKET DYNLNITYGT
160 170 180 190 200
GGANGIYFRD SITVGGATVK QQTLAYVDNV SGPTAEQSPD SELFLDGMFG
210 220 230 240 250
AAYPDNTAME AEYGDTYNTV HVNLYKQGLI SSPVFSVYMN TNDGGGQVVF
260 270 280 290 300
GGANNTLLGG DIQYTDVLKS RGGYFFWDAP VTGVKIDGAD AVSFDGAQAF
310 320 330 340 350
TIDTGTNFFI APSSFAEKVV KAALPDATES QQGYTVPCSK YQDSKTTFSL
360 370 380 390 400
VLQKSGSSSD TIDVSVPISK MLLPVDKSGE TCMFIVLPDG GNQFIVGNLF
410 420
LRFFVNVYDF GKNRIGFAPL ASGYENN
Length:427
Mass (Da):45,646
Last modified:January 1, 1990 - v2
Checksum:iDB560157A5D99BCF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti427 – 4271N → D AA sequence (PubMed:3042459).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06219 Genomic DNA. Translation: CAA29568.1. Sequence problems.
PIRiA25767.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06219 Genomic DNA. Translation: CAA29568.1. Sequence problems.
PIRiA25767.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MPPX-ray2.00A67-426[»]
ProteinModelPortaliP09177.
SMRiP09177. Positions 71-427.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA01.013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP09177.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARP_RHIPU
AccessioniPrimary (citable) accession number: P09177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 1, 1990
Last modified: April 13, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.