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Protein

Mucorpepsin

Gene
N/A
Organism
Rhizomucor pusillus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme, capable of clotting milk is frequently used for cheese production.

Catalytic activityi

Hydrolysis of proteins, favoring hydrophobic residues at P1 and P1'. Clots milk. Does not accept Lys at P1, and hence does not activate trypsinogen.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1041
Active sitei3031

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Mucorpepsin (EC:3.4.23.23)
Alternative name(s):
Mucor rennin
OrganismiRhizomucor pusillus
Taxonomic identifieri4840 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMucoromycotinaMucoralesLichtheimiaceaeRhizomucor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
PropeptideiPRO_000002589523 – 66Activation peptide1 PublicationAdd BLAST44
ChainiPRO_000002589667 – 427MucorpepsinAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi117 ↔ 123
Glycosylationi254N-linked (GlcNAc...)1
Disulfide bondi338 ↔ 382

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP09177.

Structurei

Secondary structure

1427
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi73 – 80Combined sources8
Turni81 – 84Combined sources4
Beta strandi85 – 92Combined sources8
Turni93 – 96Combined sources4
Beta strandi97 – 104Combined sources8
Beta strandi111 – 114Combined sources4
Helixi119 – 121Combined sources3
Helixi131 – 133Combined sources3
Beta strandi138 – 147Combined sources10
Beta strandi152 – 164Combined sources13
Beta strandi167 – 182Combined sources16
Helixi183 – 185Combined sources3
Beta strandi197 – 200Combined sources4
Helixi204 – 206Combined sources3
Helixi208 – 213Combined sources6
Helixi220 – 226Combined sources7
Beta strandi229 – 238Combined sources10
Beta strandi242 – 252Combined sources11
Helixi255 – 257Combined sources3
Beta strandi258 – 260Combined sources3
Beta strandi263 – 266Combined sources4
Beta strandi268 – 270Combined sources3
Beta strandi273 – 286Combined sources14
Beta strandi289 – 303Combined sources15
Beta strandi308 – 312Combined sources5
Helixi313 – 323Combined sources11
Beta strandi328 – 330Combined sources3
Beta strandi333 – 337Combined sources5
Helixi338 – 341Combined sources4
Beta strandi347 – 353Combined sources7
Beta strandi361 – 367Combined sources7
Helixi368 – 371Combined sources4
Beta strandi372 – 374Combined sources3
Beta strandi376 – 379Combined sources4
Beta strandi381 – 392Combined sources12
Beta strandi395 – 397Combined sources3
Helixi398 – 401Combined sources4
Beta strandi404 – 409Combined sources6
Turni410 – 413Combined sources4
Beta strandi414 – 420Combined sources7
Turni422 – 424Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MPPX-ray2.00A67-426[»]
ProteinModelPortaliP09177.
SMRiP09177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09177.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 418Peptidase A1PROSITE-ProRule annotationAdd BLAST333

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

KOiK01386.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFSKISSAI LLTAASFALT SARPVSKQSD ADDKLLALPL TSVNRKYSQT
60 70 80 90 100
KHGQQAAEKL GGIKAFAEGD GSVDTPGLYD FDLEEYAIPV SIGTPGQDFY
110 120 130 140 150
LLFDTGSSDT WVPHKGCDNS EGCVGKRFFD PSSSSTFKET DYNLNITYGT
160 170 180 190 200
GGANGIYFRD SITVGGATVK QQTLAYVDNV SGPTAEQSPD SELFLDGMFG
210 220 230 240 250
AAYPDNTAME AEYGDTYNTV HVNLYKQGLI SSPVFSVYMN TNDGGGQVVF
260 270 280 290 300
GGANNTLLGG DIQYTDVLKS RGGYFFWDAP VTGVKIDGAD AVSFDGAQAF
310 320 330 340 350
TIDTGTNFFI APSSFAEKVV KAALPDATES QQGYTVPCSK YQDSKTTFSL
360 370 380 390 400
VLQKSGSSSD TIDVSVPISK MLLPVDKSGE TCMFIVLPDG GNQFIVGNLF
410 420
LRFFVNVYDF GKNRIGFAPL ASGYENN
Length:427
Mass (Da):45,646
Last modified:January 1, 1990 - v2
Checksum:iDB560157A5D99BCF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti427N → D AA sequence (PubMed:3042459).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06219 Genomic DNA. Translation: CAA29568.1. Sequence problems.
PIRiA25767.

Genome annotation databases

KEGGiag:CAA29568.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06219 Genomic DNA. Translation: CAA29568.1. Sequence problems.
PIRiA25767.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MPPX-ray2.00A67-426[»]
ProteinModelPortaliP09177.
SMRiP09177.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA01.013.

Proteomic databases

PRIDEiP09177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA29568.

Phylogenomic databases

KOiK01386.

Miscellaneous databases

EvolutionaryTraceiP09177.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARP_RHIPU
AccessioniPrimary (citable) accession number: P09177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 1, 1990
Last modified: November 30, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.