P09166 (AERA_AERTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aerolysin

Gene names

Name:

aerA

Organism

Aeromonas trota

Taxonomic identifier

29489 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Aerolysin is a cytolytic toxin exported by the Gram negative Aeromonas bacteria. The mature toxin binds to eukaryotic cells and aggregates to form holes approximately 3 nm in diameter, leading to destruction of the membrane permeability barrier and osmotic lysis By similarity.
Subunit structure	Homodimer in solution; heptamer in the membrane By similarity.
Subcellular location	Secreted. Host cell membrane.
Sequence similarities	Belongs to the aerolysin family.
Caution	Was originally thought to originate from A.sobria.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis
Cellular component	Host cell membrane Host membrane Membrane Secreted
Domain	Signal
Molecular function	Toxin
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 445	424	Aerolysin		PRO_0000035632
	Propeptide	446 – 492	47			PRO_0000035633
Amino acid modifications
	Disulfide bond	42 ↔ 98		By similarity
	Disulfide bond	182 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09166 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 520F25D2B6CA9A38	FASTA	492	54,493
	10 20 30 40 50 60 MKALKITGLS LIISATLAAQ TNAAEPIYPD QLRLFSLGED VCGTDYRPIN REEAQSVRNN 70 80 90 100 110 120 IVAMMGQWQI SGLANNWVIL GPGYNGEIKP GKASTTWCYP TRPATAEIPV LPAFNIPDGD 130 140 150 160 170 180 AVDVQWRMVH DSANFIKPVS YLAHYLGYAW VGGDHSQFVG DDMDVIQEGD DWVLRGNDGG 190 200 210 220 230 240 KCDGYRCNEK SSIRVSNFAY TLDPGSFSHG DVTQSERTLV HTVVGWATNI SDTPQSGYDV 250 260 270 280 290 300 TLNYTTMSNW SKTNTYGLSE KVSTKNKFKW PLVGETEVSI EIAANQSWAS QNGGAVTTAL 310 320 330 340 350 360 SQSVRPVVPA RSRVPVKIEL YKANISYPYE FKADMSYDLT FNGFLRWGGN AWHTHPEDRP 370 380 390 400 410 420 TLSHTFAIGP FKDKASSIRY QWDKRYLPGE MKWWDWNWAI QQNGLATMQD SLARVLRPVR 430 440 450 460 470 480 ASITGDFRAE SQFAGNIEIG TPVPLGSDSK VRRTRSVDGA NTGLKLDIPL DAQELAELGF 490 ENVTLSVTPA RN

References

[1]

"Nucleotide sequence and transcriptional analysis of the aerCaerA region of Aeromonas sobria encoding aerolysin and its regulatory region." Husslein V., Huhle B., Jarchau T., Lurz R., Goebel W., Chakraborty T. Mol. Microbiol. 2:507-517(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AB3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00559 Genomic DNA. Translation: CAA68642.1.
3D structure databases
ProteinModelPortal	P09166.
SMR	P09166. Positions 25-490.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.170.15.10. 1 hit. 3.10.40.10. 1 hit.
InterPro	IPR005832. Aerolysin. IPR023307. Aerolysin-like_beta_cmplx_dom. IPR005831. Aerolysin/haemolysin_CS. IPR005830. Aerolysn_hemolysn_leukocdn_tox. IPR005138. APT. IPR016187. C-type_lectin_fold. [Graphical view]
Pfam	PF01117. Aerolysin. 1 hit. PF03440. APT. 1 hit. [Graphical view]
PRINTS	PR00754. AEROLYSIN.
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00274. AEROLYSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AERA_AERTR

Accession

Primary (citable) accession number: P09166

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program