P09151 (LEU1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-isopropylmalate synthase
EC=2.3.3.13

Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase

Gene names

Name:	leuA
Ordered Locus Names:	b0074, JW0073

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). HAMAP MF_01025
Catalytic activity	Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP MF_01025
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP MF_01025
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from direct assay. Source: EcoCyc
Molecular function	2-isopropylmalate synthase activity Inferred from electronic annotation. Source: EC acetyl-CoA C-acetyltransferase activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 523	522	2-isopropylmalate synthase HAMAP MF_01025		PRO_0000140351

Sequences

	Sequence		Length	Mass (Da)
	P09151 [UniParc]. Last modified January 23, 2007. Version 5. Checksum: 9F6DE8C007F85FB1	FASTA	523	57,298
	10 20 30 40 50 60 MSQQVIIFDT TLRDGEQALQ ASLSVKEKLQ IALALERMGV DVMEVGFPVS SPGDFESVQT 70 80 90 100 110 120 IARQVKNSRV CALARCVEKD IDVAAESLKV AEAFRIHTFI ATSPMHIATK LRSTLDEVIE 130 140 150 160 170 180 RAIYMVKRAR NYTDDVEFSC EDAGRTPIAD LARVVEAAIN AGATTINIPD TVGYTMPFEF 190 200 210 220 230 240 AGIISGLYER VPNIDKAIIS VHTHDDLGLA VGNSLAAVHA GARQVEGAMN GIGERAGNCS 250 260 270 280 290 300 LEEVIMAIKV RKDILNVHTA INHQEIWRTS QLVSQICNMP IPANKAIVGS GAFAHSSGIH 310 320 330 340 350 360 QDGVLKNREN YEIMTPESIG LNQIQLNLTS RSGRAAVKHR MDEMGYKESE YNLDNLYDAF 370 380 390 400 410 420 LKLADKKGQV FDYDLEALAF IGKQQEEPEH FRLDYFSVQS GSNDIATAAV KLACGEEVKA 430 440 450 460 470 480 EAANGNGPVD AVYQAINRIT EYNVELVKYS LTAKGHGKDA LGQVDIVANY NGRRFHGVGL 490 500 510 520 ATDIVESSAK AMVHVLNNIW RAAEVEKELQ RKAQHNENNK ETV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 192 AND 194. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Control of leu operon expression in Escherichia coli by a transcription attenuation mechanism." Wessler S.R., Calvo J.M. J. Mol. Biol. 149:579-597(1981) [PubMed: 6171647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21. Strain: K12.
[5]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-13. Strain: K12 / EMG2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73185.1. AP009048 Genomic DNA. Translation: BAB96643.2. J01642 Genomic DNA. Translation: AAA24066.1. X55034 Genomic DNA. Translation: CAA38851.1.
PIR	B64729.
RefSeq	NP_414616.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P09151.
SMR	P09151. Positions 1-501.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6851N.
IntAct	P09151. 6 interactions.
Proteomic databases
PRIDE	P09151.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003042; EBESCP00000003042; EBESCG00000002495. EBESCT00000018402; EBESCP00000017693; EBESCG00000017456.
GeneID	947465.
GenomeReviews	Gene locus JW0073 in contig AP009048_GR. Gene locus b0074 in contig U00096_GR.
KEGG	ecj:JW0073. eco:b0074.
PATRIC	32115251. VBIEscCol129921_0077.
Organism-specific databases
EchoBASE	EB1208.
EcoGene	EG11226. leuA.
Phylogenomic databases
eggNOG	COG0119.
GeneTree	EBGT00050000011825.
HOGENOM	HBG701974.
OMA	FDEDLHA.
PhylomeDB	P09151.
ProtClustDB	PRK00915.
Enzyme and pathway databases
BioCyc	EcoCyc:2-ISOPROPYLMALATESYN-MONOMER. MetaCyc:2-ISOPROPYLMALATESYN-MONOMER.
Gene expression databases
Genevestigator	P09151.
Family and domain databases
HAMAP	MF_01025. LeuA_type1. [Tree]
InterPro	IPR013709. 2-isopropylmalate_synth_dimer. IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR005671. LeuA_bact_synth. IPR000891. PYR_CT. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01649.
Pfam	PF00682. HMGL-like. 1 hit. PF08502. LeuA_dimer. 1 hit. [Graphical view]
SMART	SM00917. LeuA_dimer. 1 hit. [Graphical view]
SUPFAM	SSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMs	TIGR00973. LeuA_bact. 1 hit.
PROSITE	PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit. PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU1_ECOLI

Accession

Primary (citable) accession number: P09151
Secondary accession number(s): P78044

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 110 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program