Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Galactose-1-phosphate uridylyltransferase

Gene

galT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationNote: Binds 1 Fe cation per subunit.
  • Zn2+Note: Binds 1 zinc ion per subunit.

Kineticsi

  1. KM=0.200 mM for uridine 5'-diphosphate glucose1 Publication
  2. KM=0.303 mM for galactose-1-phosphate1 Publication
  3. KM=0.121 mM for uridine 5'-diphosphate galactose1 Publication
  4. KM=0.157 mM for glucose-1-phosphate1 Publication
  1. Vmax=180 µmol/min/mg enzyme1 Publication

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52Zinc1
Metal bindingi55Zinc1
Metal bindingi115Zinc1
Metal bindingi164Zinc1
Active sitei166Tele-UMP-histidine intermediatePROSITE-ProRule annotation1 Publication1
Metal bindingi182Iron1
Metal bindingi281Iron1
Metal bindingi296Iron1
Metal bindingi298Iron1

GO - Molecular functioni

  • ferrous iron binding Source: EcoCyc
  • galactokinase activity Source: CACAO
  • UDP-glucose:hexose-1-phosphate uridylyltransferase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • galactose catabolic process via UDP-galactose Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:GALACTURIDYLYLTRANS-MONOMER.
ECOL316407:JW0741-MONOMER.
MetaCyc:GALACTURIDYLYLTRANS-MONOMER.
BRENDAi2.7.7.12. 2026.
SABIO-RKP09148.
UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactose-1-phosphate uridylyltransferase (EC:2.7.7.12)
Short name:
Gal-1-P uridylyltransferase
Alternative name(s):
UDP-glucose--hexose-1-phosphate uridylyltransferase
Gene namesi
Name:galT
Synonyms:galB
Ordered Locus Names:b0758, JW0741
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10366. galT.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi160C → S or A: Slight inhibition of enzymatic activity. 1 Publication1
Mutagenesisi161S → A: 7000-fold reduction in specific activity. 1 Publication1
Mutagenesisi166H → G: Abolishes enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001698941 – 348Galactose-1-phosphate uridylyltransferaseAdd BLAST348

Proteomic databases

PaxDbiP09148.
PRIDEiP09148.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4261703. 381 interactors.
DIPiDIP-9735N.
IntActiP09148. 1 interactor.
STRINGi511145.b0758.

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Beta strandi11 – 14Combined sources4
Turni16 – 18Combined sources3
Beta strandi21 – 24Combined sources4
Helixi28 – 30Combined sources3
Beta strandi73 – 76Combined sources4
Beta strandi95 – 97Combined sources3
Beta strandi99 – 101Combined sources3
Beta strandi104 – 111Combined sources8
Helixi119 – 121Combined sources3
Helixi124 – 144Combined sources21
Beta strandi146 – 155Combined sources10
Helixi156 – 158Combined sources3
Beta strandi164 – 174Combined sources11
Helixi177 – 193Combined sources17
Helixi197 – 208Combined sources12
Beta strandi212 – 215Combined sources4
Beta strandi217 – 222Combined sources6
Beta strandi233 – 239Combined sources7
Helixi244 – 246Combined sources3
Helixi249 – 270Combined sources22
Beta strandi276 – 281Combined sources6
Beta strandi285 – 288Combined sources4
Beta strandi296 – 300Combined sources5
Beta strandi305 – 308Combined sources4
Helixi316 – 319Combined sources4
Beta strandi323 – 326Combined sources4
Helixi328 – 336Combined sources9
Helixi343 – 346Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GUPX-ray1.80A/B/C/D1-348[»]
1GUQX-ray1.80A/B/C/D1-348[»]
1HXPX-ray1.80A/B1-348[»]
1HXQX-ray1.86A/B1-348[»]
ProteinModelPortaliP09148.
SMRiP09148.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09148.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F0Z. Bacteria.
COG1085. LUCA.
HOGENOMiHOG000230490.
InParanoidiP09148.
KOiK00965.
OMAiGQWVLVC.
PhylomeDBiP09148.

Family and domain databases

Gene3Di3.30.428.10. 2 hits.
InterProiIPR001937. GalP_UDPtransf1.
IPR019779. GalP_UDPtransf1_His-AS.
IPR005850. GalP_Utransf_C.
IPR005849. GalP_Utransf_N.
IPR011146. HIT-like.
[Graphical view]
PANTHERiPTHR11943. PTHR11943. 1 hit.
PfamiPF02744. GalP_UDP_tr_C. 1 hit.
PF01087. GalP_UDP_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000808. GalT. 1 hit.
SUPFAMiSSF54197. SSF54197. 2 hits.
TIGRFAMsiTIGR00209. galT_1. 1 hit.
PROSITEiPS00117. GAL_P_UDP_TRANSF_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQFNPVDHP HRRYNPLTGQ WILVSPHRAK RPWQGAQETP AKQVLPAHDP
60 70 80 90 100
DCFLCAGNVR VTGDKNPDYT GTYVFTNDFA ALMSDTPDAP ESHDPLMRCQ
110 120 130 140 150
SARGTSRVIC FSPDHSKTLP ELSVAALTEI VKTWQEQTAE LGKTYPWVQV
160 170 180 190 200
FENKGAAMGC SNPHPHGQIW ANSFLPNEAE REDRLQKEYF AEQKSPMLVD
210 220 230 240 250
YVQRELADGS RTVVETEHWL AVVPYWAAWP FETLLLPKAH VLRITDLTDA
260 270 280 290 300
QRSDLALALK KLTSRYDNLF QCSFPYSMGW HGAPFNGEEN QHWQLHAHFY
310 320 330 340
PPLLRSATVR KFMVGYEMLA ETQRDLTAEQ AAERLRAVSD IHFRESGV
Length:348
Mass (Da):39,646
Last modified:November 1, 1997 - v2
Checksum:i3D55D2CB38D8C9A2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29 – 31AKR → LS in CAA29574 (PubMed:3022232).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06226 Genomic DNA. Translation: CAA29574.1.
U00096 Genomic DNA. Translation: AAC73845.1.
AP009048 Genomic DNA. Translation: BAA35420.1.
X02306 Genomic DNA. Translation: CAA26171.1.
PIRiS00722. XNECUD.
RefSeqiNP_415279.1. NC_000913.3.
WP_000191497.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73845; AAC73845; b0758.
BAA35420; BAA35420; BAA35420.
GeneIDi945357.
KEGGiecj:JW0741.
eco:b0758.
PATRICi32116717. VBIEscCol129921_0784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06226 Genomic DNA. Translation: CAA29574.1.
U00096 Genomic DNA. Translation: AAC73845.1.
AP009048 Genomic DNA. Translation: BAA35420.1.
X02306 Genomic DNA. Translation: CAA26171.1.
PIRiS00722. XNECUD.
RefSeqiNP_415279.1. NC_000913.3.
WP_000191497.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GUPX-ray1.80A/B/C/D1-348[»]
1GUQX-ray1.80A/B/C/D1-348[»]
1HXPX-ray1.80A/B1-348[»]
1HXQX-ray1.86A/B1-348[»]
ProteinModelPortaliP09148.
SMRiP09148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261703. 381 interactors.
DIPiDIP-9735N.
IntActiP09148. 1 interactor.
STRINGi511145.b0758.

Proteomic databases

PaxDbiP09148.
PRIDEiP09148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73845; AAC73845; b0758.
BAA35420; BAA35420; BAA35420.
GeneIDi945357.
KEGGiecj:JW0741.
eco:b0758.
PATRICi32116717. VBIEscCol129921_0784.

Organism-specific databases

EchoBASEiEB0361.
EcoGeneiEG10366. galT.

Phylogenomic databases

eggNOGiENOG4105F0Z. Bacteria.
COG1085. LUCA.
HOGENOMiHOG000230490.
InParanoidiP09148.
KOiK00965.
OMAiGQWVLVC.
PhylomeDBiP09148.

Enzyme and pathway databases

UniPathwayiUPA00214.
BioCyciEcoCyc:GALACTURIDYLYLTRANS-MONOMER.
ECOL316407:JW0741-MONOMER.
MetaCyc:GALACTURIDYLYLTRANS-MONOMER.
BRENDAi2.7.7.12. 2026.
SABIO-RKP09148.

Miscellaneous databases

EvolutionaryTraceiP09148.
PROiP09148.

Family and domain databases

Gene3Di3.30.428.10. 2 hits.
InterProiIPR001937. GalP_UDPtransf1.
IPR019779. GalP_UDPtransf1_His-AS.
IPR005850. GalP_Utransf_C.
IPR005849. GalP_Utransf_N.
IPR011146. HIT-like.
[Graphical view]
PANTHERiPTHR11943. PTHR11943. 1 hit.
PfamiPF02744. GalP_UDP_tr_C. 1 hit.
PF01087. GalP_UDP_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000808. GalT. 1 hit.
SUPFAMiSSF54197. SSF54197. 2 hits.
TIGRFAMsiTIGR00209. galT_1. 1 hit.
PROSITEiPS00117. GAL_P_UDP_TRANSF_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAL7_ECOLI
AccessioniPrimary (citable) accession number: P09148
Secondary accession number(s): P78270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.