P09146 (OMPA_ENTAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer membrane protein A

Gene names

Name:

ompA

Organism

Enterobacter aerogenes (Aerobacter aerogenes)

Taxonomic identifier

548 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the action of colicins K and L and for the stabilization of mating aggregates in conjugation. Serves as a receptor for a number of T-even like phages. Also acts as a porin with low permeability that allows slow penetration of small solutes By similarity.
Subunit structure	Monomer Probable.
Subcellular location	Cell outer membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the OmpA family. Contains 1 OmpA-like domain.

Ontologies

Keywords
Biological process	Conjugation Ion transport Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Repeat Signal Transmembrane Transmembrane beta strand
Molecular function	Porin
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	conjugation Inferred from electronic annotation. Source: UniProtKB-KW ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW pore complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	porin activity Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 350	329	Outer membrane protein A		PRO_0000020096
Regions
	Transmembrane	27 – 40	14	Beta stranded; Potential
	Transmembrane	59 – 71	13	Beta stranded; Potential
	Transmembrane	74 – 89	16	Beta stranded; Potential
	Transmembrane	101 – 111	11	Beta stranded; Potential
	Transmembrane	115 – 130	16	Beta stranded; Potential
	Transmembrane	146 – 157	12	Beta stranded; Potential
	Transmembrane	163 – 179	17	Beta stranded; Potential
	Transmembrane	185 – 196	12	Beta stranded; Potential
	Repeat	205 – 206	2	1
	Repeat	207 – 208	2	2
	Repeat	209 – 210	2	3
	Repeat	211 – 212	2	4
	Domain	214 – 342	129	OmpA-like
	Region	205 – 212	8	4 X 2 AA tandem repeats of A-P
Amino acid modifications
	Disulfide bond	315 ↔ 327		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09146 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 6276C6F2F21065DA	FASTA	350	37,575
	10 20 30 40 50 60 MKKTAIAIAV ALAGFATVAQ AAPKDNTWYA GGKLGWSQFH DTGWYNSNLN NNGPTHESQL 70 80 90 100 110 120 GAGAFGGYQV NPYLGFEMGY DWLGRMPYKG VKVNGAFSSQ AVQLTAKLGY PITDDLDIYT 130 140 150 160 170 180 RLGGMVWRAD SSNSIAGDNH DTGVSPVFAG GVEWAMTRDI ATRLEYQWVN NIGDAGTVGV 190 200 210 220 230 240 RPDNGMLSVG VSYRFGQEDN APVVAPAPAP APEVTTKTFT LKSDVLFNFN KATLKPEGQQ 250 260 270 280 290 300 ALDQLYTQLS NMDPKDGSAV VLGYTDRIGS EQYNQKLSEK RAQSVVDYLV AKGIPANKIS 310 320 330 340 350 ARGMGESDPV TGNTCDNVKA RAALIDCLAP DRRVAIEVKG YKDVVTQPQA

References

[1]	"Molecular characterization of the gene coding for major outer membrane protein OmpA from Enterobacter aerogenes." Braun G., Cole S.T. Eur. J. Biochem. 137:495-500(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X00254 Genomic DNA. Translation: CAA25062.1.
PIR	S07222.
3D structure databases
ProteinModelPortal	P09146.
SMR	P09146. Positions 22-196.
ModBase	Search...
Proteomic databases
PRIDE	P09146.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.40.160.20. 1 hit. 3.30.1330.60. 1 hit.
InterPro	IPR011250. OMP/PagP_b-brl. IPR006664. OMP_bac. IPR002368. OmpA. IPR006690. OMPA-like_CS. IPR000498. OmpA-like_TM_dom. IPR006665. OmpA/MotB_C. [Graphical view]
Pfam	PF00691. OmpA. 1 hit. PF01389. OmpA_membrane. 1 hit. [Graphical view]
PRINTS	PR01021. OMPADOMAIN. PR01022. OUTRMMBRANEA.
SUPFAM	SSF103088. OmpA/MotB_C. 1 hit. SSF56925. SSF56925. 1 hit.
PROSITE	PS01068. OMPA_1. 1 hit. PS51123. OMPA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OMPA_ENTAE

Accession

Primary (citable) accession number: P09146

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program