Reviewed, UniProtKB/Swiss-Prot P09142 (LUXF_PHOLE)

Names and origin

Protein names

Recommended name:
    Non-fluorescent flavoprotein
      Short name=NFP
Alternative name(s):
    FP390

Gene names

Name:

luxF

Organism

Photobacterium leiognathi

Taxonomic identifier

658 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Binds 2 FMN per subunit. Each FMN has a myristate attached.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.
Caution	Was originally termed luxG.

Ontologies

Keywords
Biological process	Luminescence
Ligand	Flavoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	monooxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 228

228

Non-fluorescent flavoprotein

PRO_0000220182

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

228

Helix Strand Turn

Details...

Beta strand

6 – 11

6

Helix

21 – 37

17

Beta strand

42 – 48

7

Beta strand

65 – 68

4

Beta strand

71 – 75

5

Helix

79 – 87

9

Beta strand

92 – 94

3

Helix

100 – 117

18

Beta strand

126 – 134

9

Helix

138 – 156

19

Helix

162 – 167

6

Beta strand

169 – 172

4

Helix

174 – 187

14

Turn

188 – 190

3

Beta strand

192 – 197

6

Helix

204 – 224

21

Sequences

	Sequence		Length	Mass (Da)
	P09142-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: A4AFDB334B12B54B	FASTA	228	26,239
	10 20 30 40 50 60 MTKWNYGVFF LNFYHVGQQE PSLTMSNALE TLRIIDEDTS IYDVVAFSEH HIDKSYNDET 70 80 90 100 110 120 KLAPFVSLGK QIHILATSPE TVVKAAKYGM PLLFKWDDSQ QKRIELLNHY QAAAAKFNVD 130 140 150 160 170 180 IAGVRHRLML FVNVNDNPTQ AKAELSIYLE DYLSYTQAET SIDEIINSNA AGNFDTCLHH 190 200 210 220 VAEMAQGLNN KVDFLFCFES MKDQENKKSL MINFDKRVIN YRKEHNLN

References

[1]	"Nucleotide sequence of part of Photobacterium leiognathi lux region." Illiarinov B.A., Protopopova M.V., Karginov V.A., Mertvetsov N.P., Gitelson J.I. Nucleic Acids Res. 16:9855-9855(1988) [PubMed: 3186447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 554.
[2]	"Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes." Illarionov B.A., Blinov V.M., Donchenko A.P., Protopopova M.V., Karginov V.A., Mertvetsov N.P., Gitelson J.I. Gene 86:89-94(1990) [PubMed: 2311938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 554.
[3]	"Crystal structure of a flavoprotein related to the subunits of bacterial luciferase." Moore S.A., James M.N.G., O'Kane D.J., Lee J. EMBO J. 12:1767-1774(1993) [PubMed: 8491169] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[4]	"Structural refinement of the non-fluorescent flavoprotein from Photobacterium leiognathi at 1.60-A resolution." Moore S.A., James M.N.G. J. Mol. Biol. 249:195-214(1995) [PubMed: 7776372] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

X08036 Genomic DNA. Translation: CAA30833.1.

PIR

JQ0415.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NFP	X-ray	1.60	A	1-228	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like.
IPR016048. Luciferase-like_sub.
IPR002103. Luciferase_bac.
[Graphical view]

Gene3D

G3DSA:3.20.20.30. Luciferase_like. 1 hit.

Pfam

PF00296. Bac_luciferase. 1 hit.
[Graphical view]

PRINTS

PR00089. LUCIFERASE.

PROSITE

PS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LUXF_PHOLE

Accession

Primary (citable) accession number: P09142

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)