ID   LUXB1_PHOLE             Reviewed;         325 AA.
AC   P09141;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   16-JUN-2009, entry version 56.
DE   RecName: Full=Alkanal monooxygenase beta chain;
DE            EC=1.14.14.3;
DE   AltName: Full=Bacterial luciferase beta chain;
GN   Name=luxB;
OS   Photobacterium leiognathi.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Photobacterium.
OX   NCBI_TaxID=658;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=554;
RX   MEDLINE=89041570; PubMed=3186447; DOI=10.1093/nar/16.20.9855;
RA   Illiarinov B.A., Protopopova M.V., Karginov V.A., Mertvetsov N.P.,
RA   Gitelson J.I.;
RT   "Nucleotide sequence of part of Photobacterium leiognathi lux
RT   region.";
RL   Nucleic Acids Res. 16:9855-9855(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=554;
RX   MEDLINE=88251495; PubMed=3382442;
RA   Illarionov B.A., Protopopova M.V., Karginov V.A., Mertvetsov N.P.,
RA   Gitelson J.I.;
RT   "Nucleotide sequence of genes for alpha- and beta-subunits of
RT   luciferase from Photobacterium leiognathi.";
RL   Bioorg. Khim. 14:412-415(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=554;
RX   MEDLINE=90185252; PubMed=2311938; DOI=10.1016/0378-1119(90)90117-A;
RA   Illarionov B.A., Blinov V.M., Donchenko A.P., Protopopova M.V.,
RA   Karginov V.A., Mertvetsov N.P., Gitelson J.I.;
RT   "Isolation of bioluminescent functions from Photobacterium leiognathi:
RT   analysis of luxA, luxB, luxG and neighboring genes.";
RL   Gene 86:89-94(1990).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria. The
CC       specific role of the beta subunit is unknown, but it is absolutely
CC       required for bioluminescence activity.
CC   -!- CATALYTIC ACTIVITY: RCHO + reduced FMN + O(2) = RCOOH + FMN +
CC       H(2)O + light.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC   -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase
CC       family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X08036; CAA30832.1; -; Genomic_DNA.
DR   PIR; S01698; S01698.
DR   HSSP; P07739; 1BSL.
DR   BRENDA; 1.14.14.3; 349.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR018235; Bacterial_luciferase_CS.
DR   InterPro; IPR011251; Luciferase-like.
DR   InterPro; IPR016048; Luciferase-like_sub.
DR   InterPro; IPR002103; Luciferase_bac.
DR   Gene3D; G3DSA:3.20.20.30; Luciferase_like; 1.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   PRINTS; PR00089; LUCIFERASE.
DR   PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW   Photoprotein.
FT   CHAIN         1    325       Alkanal monooxygenase beta chain.
FT                                /FTId=PRO_0000220174.
SQ   SEQUENCE   325 AA;  37353 MW;  73A7AFA4F92CF2A9 CRC64;
     MNFGLFFLNF QLKGMTSEAV LDNMIDTIAL VDKDEYHFKT AFVNEHHFSK NGIVGAPMTA
     ASFLLGLTER LHIGSLNQVI TTHHPVRIAE EASLLDQMSD GRFILGLSDC VSDFEMDFFK
     RQRDSQQQQF EACYEILNDG ITTNYCYANN DFYNFPKISI NPHCISKENL KQYILATSMG
     VVEWAAKKGL PLTYRWSDTL AEKENYYQRY LTVAAENNVD ITHVDHQFPL LVNINPDRDI
     AKQEMRDYIR GYIAEAYPNT DQEEKIEELI KQHAVGTEDE YYESSKYALE KTGSKNVLLS
     FESMKNKAAV IDLINMVNEK IKKNL
//