Reviewed, UniProtKB/Swiss-Prot P09141 (LUXB1_PHOLE)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase beta chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase beta chain

Gene names

Name:

luxB

Organism

Photobacterium leiognathi

Taxonomic identifier

553611 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Alkanal monooxygenase beta chain		PRO_0000220174

Sequences

	Sequence		Length	Mass (Da)
	P09141-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 73A7AFA4F92CF2A9	FASTA	325	37,353
	10 20 30 40 50 60 MNFGLFFLNF QLKGMTSEAV LDNMIDTIAL VDKDEYHFKT AFVNEHHFSK NGIVGAPMTA 70 80 90 100 110 120 ASFLLGLTER LHIGSLNQVI TTHHPVRIAE EASLLDQMSD GRFILGLSDC VSDFEMDFFK 130 140 150 160 170 180 RQRDSQQQQF EACYEILNDG ITTNYCYANN DFYNFPKISI NPHCISKENL KQYILATSMG 190 200 210 220 230 240 VVEWAAKKGL PLTYRWSDTL AEKENYYQRY LTVAAENNVD ITHVDHQFPL LVNINPDRDI 250 260 270 280 290 300 AKQEMRDYIR GYIAEAYPNT DQEEKIEELI KQHAVGTEDE YYESSKYALE KTGSKNVLLS 310 320 FESMKNKAAV IDLINMVNEK IKKNL

References

[1]	"Nucleotide sequence of part of Photobacterium leiognathi lux region." Illiarinov B.A., Protopopova M.V., Karginov V.A., Mertvetsov N.P., Gitelson J.I. Nucleic Acids Res. 16:9855-9855(1988) [PubMed: 3186447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 554.
[2]	"Nucleotide sequence of genes for alpha- and beta-subunits of luciferase from Photobacterium leiognathi." Illarionov B.A., Protopopova M.V., Karginov V.A., Mertvetsov N.P., Gitelson J.I. Bioorg. Khim. 14:412-415(1988) [PubMed: 3382442] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 554.
[3]	"Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes." Illarionov B.A., Blinov V.M., Donchenko A.P., Protopopova M.V., Karginov V.A., Mertvetsov N.P., Gitelson J.I. Gene 86:89-94(1990) [PubMed: 2311938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 554.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X08036 Genomic DNA. Translation: CAA30832.1.
PIR	S01698.
3D structure databases
HSSP	HSSP built from PDB template 1BSL based on UniProtKB P07739.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.14.3. 349.
Family and domain databases
InterPro	IPR018235. Bacterial_luciferase_CS. IPR011251. Luciferase-like. IPR016048. Luciferase-like_sub. IPR002103. Luciferase_bac. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
PRINTS	PR00089. LUCIFERASE.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXB1_PHOLE

Accession

Primary (citable) accession number: P09141

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	September 1, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)