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Protein

Serine--pyruvate aminotransferase, mitochondrial

Gene

Agxt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).

Catalytic activityi

L-serine + pyruvate = 3-hydroxypyruvate + L-alanine.
L-alanine + glyoxylate = pyruvate + glycine.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei382 – 3821SubstrateBy similarity

GO - Molecular functioni

  1. alanine-glyoxylate transaminase activity Source: RGD
  2. amino acid binding Source: RGD
  3. pyridoxal phosphate binding Source: Ensembl
  4. serine-pyruvate transaminase activity Source: RGD

GO - Biological processi

  1. glycine biosynthetic process, by transamination of glyoxylate Source: RGD
  2. glyoxylate catabolic process Source: Ensembl
  3. L-alanine catabolic process Source: Ensembl
  4. L-cysteine catabolic process Source: Ensembl
  5. oxalic acid secretion Source: Ensembl
  6. pyruvate biosynthetic process Source: RGD
  7. response to cAMP Source: RGD
  8. response to glucocorticoid Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13151.
ReactomeiREACT_314137. Glyoxylate metabolism.
SABIO-RKP09139.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--pyruvate aminotransferase, mitochondrial (EC:2.6.1.51)
Short name:
SPT
Alternative name(s):
Alanine--glyoxylate aminotransferase (EC:2.6.1.44)
Short name:
AGT
Gene namesi
Name:Agxt
Synonyms:Agt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi2073. Agxt.

Subcellular locationi

  1. Mitochondrion matrix
  2. Peroxisome

  3. Note: Induced on glucagon administration. Not affected by glucagon.

GO - Cellular componenti

  1. mitochondrial matrix Source: HGNC
  2. mitochondrion Source: HGNC
  3. peroxisomal matrix Source: Ensembl
  4. peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2323MitochondrionAdd
BLAST
Chaini24 – 414391Serine--pyruvate aminotransferase, mitochondrialPRO_0000001290Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei231 – 2311N6-(pyridoxal phosphate)lysineBy similarity
Modified residuei247 – 2471N6-acetyllysine; alternateBy similarity
Modified residuei247 – 2471N6-succinyllysine; alternateBy similarity
Modified residuei256 – 2561N6-acetyllysineBy similarity
Modified residuei334 – 3341N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP09139.
PRIDEiP09139.

Expressioni

Gene expression databases

ExpressionAtlasiP09139. baseline.
GenevestigatoriP09139.

Structurei

3D structure databases

ProteinModelPortaliP09139.
SMRiP09139. Positions 26-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi412 – 4143Microbody targeting signalBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0075.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171815.
HOVERGENiHBG006907.
InParanoidiP09139.
KOiK00830.
OMAiAYSCSQK.
OrthoDBiEOG7CZK6B.
PhylomeDBiP09139.
TreeFamiTF313234.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P09139-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRMLAKASV TLGSRAASWV RNMGSHQLLV PPPEALSKPL SIPKRLLLGP
60 70 80 90 100
GPSNLAPRVL AAGSLRMIGH MQKEMFQIMD EIKQGIQYVF QTRNPLTLVV
110 120 130 140 150
SGSGHCAMET ALFNLLEPGD SFLVGTNGIW GIRAAEIAER IGARVHQMIK
160 170 180 190 200
KPGEHYTLQE VEEGLAQHKP VLLFLTHGES STGVLQPLDG FGELCHRYQC
210 220 230 240 250
LLLVDSVASL GGVPIYMDQQ GIDILYSGSQ KVLNAPPGIS LISFNDKAKS
260 270 280 290 300
KVYSRKTKPV SFYTDITYLS KLWGCEGKTR VIHHTLPVIS LYCLRESLAL
310 320 330 340 350
ISEQGLENSW RRHREATAHL HKCLRELGLK FFVKDPEIRL PTITTVTVPA
360 370 380 390 400
GYNWRDIVSY VLDHFNIEIS GGLGPSEDKV LRIGLLGYNA TTENADRVAE
410
ALREALQHCP KNKL
Length:414
Mass (Da):45,834
Last modified:July 1, 1989 - v1
Checksum:i3FBD66ED36C3D9EF
GO
Isoform Peroxisomal (identifier: P09139-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:392
Mass (Da):43,399
Checksum:i5A42F40FD95B6E3E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222Missing in isoform Peroxisomal. CuratedVSP_018645Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06357 mRNA. Translation: CAA29656.1.
D13667 Genomic DNA. Translation: BAA02838.1.
M35270 mRNA. Translation: AAA42169.1.
PIRiS00164. XNRTSP.
RefSeqiNP_001263635.1. NM_001276706.1. [P09139-2]
NP_085914.2. NM_030656.2. [P09139-1]
UniGeneiRn.9931.

Genome annotation databases

EnsembliENSRNOT00000029127; ENSRNOP00000030340; ENSRNOG00000023856. [P09139-1]
GeneIDi24792.
KEGGirno:24792.
UCSCiRGD:2073. rat. [P09139-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06357 mRNA. Translation: CAA29656.1.
D13667 Genomic DNA. Translation: BAA02838.1.
M35270 mRNA. Translation: AAA42169.1.
PIRiS00164. XNRTSP.
RefSeqiNP_001263635.1. NM_001276706.1. [P09139-2]
NP_085914.2. NM_030656.2. [P09139-1]
UniGeneiRn.9931.

3D structure databases

ProteinModelPortaliP09139.
SMRiP09139. Positions 26-411.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP09139.
PRIDEiP09139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029127; ENSRNOP00000030340; ENSRNOG00000023856. [P09139-1]
GeneIDi24792.
KEGGirno:24792.
UCSCiRGD:2073. rat. [P09139-1]

Organism-specific databases

CTDi189.
RGDi2073. Agxt.

Phylogenomic databases

eggNOGiCOG0075.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171815.
HOVERGENiHBG006907.
InParanoidiP09139.
KOiK00830.
OMAiAYSCSQK.
OrthoDBiEOG7CZK6B.
PhylomeDBiP09139.
TreeFamiTF313234.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13151.
ReactomeiREACT_314137. Glyoxylate metabolism.
SABIO-RKP09139.

Miscellaneous databases

NextBioi604430.
PROiP09139.

Gene expression databases

ExpressionAtlasiP09139. baseline.
GenevestigatoriP09139.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the cDNA encoding the precursor for mitochondrial serine:pyruvate aminotransferase of rat liver."
    Oda T., Miyajima H., Suzuki Y., Ichiyama A.
    Eur. J. Biochem. 168:537-542(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  2. "Characterization and sequence analysis of rat serine:pyruvate/alanine:glyoxylate aminotransferase gene."
    Oda T., Nishiyama K., Ichiyama A.
    Genomics 17:59-65(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-141.
    Strain: Wistar.
  3. "Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine:pyruvate aminotransferase of rat liver."
    Oda T., Funai T., Ichiyama A.
    J. Biol. Chem. 265:7513-7519(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE INITIATION.

Entry informationi

Entry nameiSPYA_RAT
AccessioniPrimary (citable) accession number: P09139
Secondary accession number(s): Q9R2C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 1, 2015
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.