Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine--pyruvate aminotransferase, mitochondrial

Gene

Agxt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).

Catalytic activityi

L-serine + pyruvate = 3-hydroxypyruvate + L-alanine.
L-alanine + glyoxylate = pyruvate + glycine.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei382SubstrateBy similarity1

GO - Molecular functioni

  • alanine-glyoxylate transaminase activity Source: RGD
  • amino acid binding Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • protein self-association Source: UniProtKB
  • pyridoxal phosphate binding Source: Ensembl
  • serine-pyruvate transaminase activity Source: RGD

GO - Biological processi

  • glycine biosynthetic process, by transamination of glyoxylate Source: RGD
  • glyoxylate catabolic process Source: Ensembl
  • L-alanine catabolic process Source: Ensembl
  • L-cysteine catabolic process Source: Ensembl
  • Notch signaling pathway Source: Ensembl
  • oxalic acid secretion Source: Ensembl
  • proteasomal protein catabolic process Source: UniProtKB
  • pyruvate biosynthetic process Source: RGD
  • response to cAMP Source: RGD
  • response to glucocorticoid Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13151.
ReactomeiR-RNO-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKP09139.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--pyruvate aminotransferase, mitochondrial (EC:2.6.1.51)
Short name:
SPT
Alternative name(s):
Alanine--glyoxylate aminotransferase (EC:2.6.1.44)
Short name:
AGT
Gene namesi
Name:Agxt
Synonyms:Agt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi2073. Agxt.

Subcellular locationi

  • Mitochondrion matrix
  • Peroxisome By similarity

  • Note: Induced on glucagon administration. Not affected by glucagon.

GO - Cellular componenti

  • mitochondrial matrix Source: HGNC
  • mitochondrion Source: HGNC
  • peroxisomal matrix Source: Ensembl
  • peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23MitochondrionAdd BLAST23
ChainiPRO_000000129024 – 414Serine--pyruvate aminotransferase, mitochondrialAdd BLAST391

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei231N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei247N6-acetyllysine; alternateBy similarity1
Modified residuei247N6-succinyllysine; alternateBy similarity1
Modified residuei256N6-acetyllysineBy similarity1
Modified residuei334N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP09139.
PRIDEiP09139.

PTM databases

iPTMnetiP09139.
PhosphoSitePlusiP09139.

Expressioni

Gene expression databases

BgeeiENSRNOG00000023856.
ExpressionAtlasiP09139. baseline and differential.
GenevisibleiP09139. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030340.

Structurei

3D structure databases

ProteinModelPortaliP09139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi412 – 414Microbody targeting signalBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2862. Eukaryota.
COG0075. LUCA.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171815.
HOVERGENiHBG006907.
InParanoidiP09139.
KOiK00830.
OMAiAYSCSQK.
OrthoDBiEOG091G0EMK.
PhylomeDBiP09139.
TreeFamiTF313234.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P09139-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRMLAKASV TLGSRAASWV RNMGSHQLLV PPPEALSKPL SIPKRLLLGP
60 70 80 90 100
GPSNLAPRVL AAGSLRMIGH MQKEMFQIMD EIKQGIQYVF QTRNPLTLVV
110 120 130 140 150
SGSGHCAMET ALFNLLEPGD SFLVGTNGIW GIRAAEIAER IGARVHQMIK
160 170 180 190 200
KPGEHYTLQE VEEGLAQHKP VLLFLTHGES STGVLQPLDG FGELCHRYQC
210 220 230 240 250
LLLVDSVASL GGVPIYMDQQ GIDILYSGSQ KVLNAPPGIS LISFNDKAKS
260 270 280 290 300
KVYSRKTKPV SFYTDITYLS KLWGCEGKTR VIHHTLPVIS LYCLRESLAL
310 320 330 340 350
ISEQGLENSW RRHREATAHL HKCLRELGLK FFVKDPEIRL PTITTVTVPA
360 370 380 390 400
GYNWRDIVSY VLDHFNIEIS GGLGPSEDKV LRIGLLGYNA TTENADRVAE
410
ALREALQHCP KNKL
Length:414
Mass (Da):45,834
Last modified:July 1, 1989 - v1
Checksum:i3FBD66ED36C3D9EF
GO
Isoform Peroxisomal (identifier: P09139-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:392
Mass (Da):43,399
Checksum:i5A42F40FD95B6E3E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186451 – 22Missing in isoform Peroxisomal. CuratedAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06357 mRNA. Translation: CAA29656.1.
D13667 Genomic DNA. Translation: BAA02838.1.
M35270 mRNA. Translation: AAA42169.1.
PIRiS00164. XNRTSP.
RefSeqiNP_001263635.1. NM_001276706.1. [P09139-2]
NP_085914.2. NM_030656.2. [P09139-1]
UniGeneiRn.9931.

Genome annotation databases

EnsembliENSRNOT00000029127; ENSRNOP00000030340; ENSRNOG00000023856. [P09139-1]
GeneIDi24792.
KEGGirno:24792.
UCSCiRGD:2073. rat. [P09139-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06357 mRNA. Translation: CAA29656.1.
D13667 Genomic DNA. Translation: BAA02838.1.
M35270 mRNA. Translation: AAA42169.1.
PIRiS00164. XNRTSP.
RefSeqiNP_001263635.1. NM_001276706.1. [P09139-2]
NP_085914.2. NM_030656.2. [P09139-1]
UniGeneiRn.9931.

3D structure databases

ProteinModelPortaliP09139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030340.

PTM databases

iPTMnetiP09139.
PhosphoSitePlusiP09139.

Proteomic databases

PaxDbiP09139.
PRIDEiP09139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029127; ENSRNOP00000030340; ENSRNOG00000023856. [P09139-1]
GeneIDi24792.
KEGGirno:24792.
UCSCiRGD:2073. rat. [P09139-1]

Organism-specific databases

CTDi189.
RGDi2073. Agxt.

Phylogenomic databases

eggNOGiKOG2862. Eukaryota.
COG0075. LUCA.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171815.
HOVERGENiHBG006907.
InParanoidiP09139.
KOiK00830.
OMAiAYSCSQK.
OrthoDBiEOG091G0EMK.
PhylomeDBiP09139.
TreeFamiTF313234.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13151.
ReactomeiR-RNO-389661. Glyoxylate metabolism and glycine degradation.
SABIO-RKP09139.

Miscellaneous databases

PROiP09139.

Gene expression databases

BgeeiENSRNOG00000023856.
ExpressionAtlasiP09139. baseline and differential.
GenevisibleiP09139. RN.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPYA_RAT
AccessioniPrimary (citable) accession number: P09139
Secondary accession number(s): Q9R2C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.