Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P09139 (SPYA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine--pyruvate aminotransferase, mitochondrial

Short name=SPT
EC=2.6.1.51
Alternative name(s):
Alanine--glyoxylate aminotransferase
Short name=AGT
EC=2.6.1.44
Gene names
Name:Agxt
Synonyms:Agt1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).

Catalytic activity

L-serine + pyruvate = 3-hydroxypyruvate + L-alanine.

L-alanine + glyoxylate = pyruvate + glycine.

Cofactor

Pyridoxal phosphate.

Subcellular location

Mitochondrion matrix. Peroxisome. Note: Induced on glucagon administration. Not affected by glucagon.

Sequence similarities

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
   Cellular componentMitochondrion
Peroxisome
   Coding sequence diversityAlternative initiation
   DomainTransit peptide
   LigandPyridoxal phosphate
   Molecular functionAminotransferase
Transferase
   PTMAcetylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processL-alanine catabolic process

Inferred from electronic annotation. Source: Ensembl

L-cysteine catabolic process

Inferred from electronic annotation. Source: Ensembl

glycine biosynthetic process, by transamination of glyoxylate

Inferred from direct assay PubMed 12383475. Source: RGD

glyoxylate catabolic process

Inferred from electronic annotation. Source: Ensembl

oxalic acid secretion

Inferred from electronic annotation. Source: Ensembl

protein targeting to peroxisome

Inferred from electronic annotation. Source: Ensembl

pyruvate biosynthetic process

Inferred from direct assay PubMed 12383475. Source: RGD

response to cAMP

Inferred from direct assay PubMed 17958. Source: RGD

response to glucocorticoid

Inferred from direct assay PubMed 167860. Source: RGD

   Cellular_componentmitochondrial matrix

Inferred from direct assay PubMed 14561759. Source: HGNC

mitochondrion

Traceable author statement PubMed 7813517. Source: HGNC

peroxisomal matrix

Inferred from electronic annotation. Source: Ensembl

peroxisome

Inferred from direct assay PubMed 14561759. Source: HGNC

   Molecular_functionalanine-glyoxylate transaminase activity

Inferred from direct assay PubMed 12383475. Source: RGD

amino acid binding

Inferred from direct assay PubMed 12383475. Source: RGD

pyridoxal phosphate binding

Inferred from electronic annotation. Source: Ensembl

serine-pyruvate transaminase activity

Inferred from direct assay PubMed 3894025. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Mitochondrial (identifier: P09139-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Peroxisomal (identifier: P09139-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2323Mitochondrion
Chain24 – 414391Serine--pyruvate aminotransferase, mitochondrial
PRO_0000001290

Regions

Motif412 – 4143Microbody targeting signal By similarity

Sites

Binding site3821Substrate By similarity

Amino acid modifications

Modified residue2311N6-(pyridoxal phosphate)lysine By similarity
Modified residue2471N6-acetyllysine; alternate By similarity
Modified residue2471N6-succinyllysine; alternate By similarity
Modified residue2561N6-acetyllysine By similarity
Modified residue3341N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 2222Missing in isoform Peroxisomal.
VSP_018645

Sequences

Sequence LengthMass (Da)Tools
Isoform Mitochondrial [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 3FBD66ED36C3D9EF

FASTA41445,834
        10         20         30         40         50         60 
MFRMLAKASV TLGSRAASWV RNMGSHQLLV PPPEALSKPL SIPKRLLLGP GPSNLAPRVL 

        70         80         90        100        110        120 
AAGSLRMIGH MQKEMFQIMD EIKQGIQYVF QTRNPLTLVV SGSGHCAMET ALFNLLEPGD 

       130        140        150        160        170        180 
SFLVGTNGIW GIRAAEIAER IGARVHQMIK KPGEHYTLQE VEEGLAQHKP VLLFLTHGES 

       190        200        210        220        230        240 
STGVLQPLDG FGELCHRYQC LLLVDSVASL GGVPIYMDQQ GIDILYSGSQ KVLNAPPGIS 

       250        260        270        280        290        300 
LISFNDKAKS KVYSRKTKPV SFYTDITYLS KLWGCEGKTR VIHHTLPVIS LYCLRESLAL 

       310        320        330        340        350        360 
ISEQGLENSW RRHREATAHL HKCLRELGLK FFVKDPEIRL PTITTVTVPA GYNWRDIVSY 

       370        380        390        400        410 
VLDHFNIEIS GGLGPSEDKV LRIGLLGYNA TTENADRVAE ALREALQHCP KNKL 

« Hide

Isoform Peroxisomal [UniParc].

Checksum: 5A42F40FD95B6E3E
Show »

FASTA39243,399

References

[1]"Nucleotide sequence of the cDNA encoding the precursor for mitochondrial serine:pyruvate aminotransferase of rat liver."
Oda T., Miyajima H., Suzuki Y., Ichiyama A.
Eur. J. Biochem. 168:537-542(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Liver.
[2]"Characterization and sequence analysis of rat serine:pyruvate/alanine:glyoxylate aminotransferase gene."
Oda T., Nishiyama K., Ichiyama A.
Genomics 17:59-65(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-141.
Strain: Wistar.
[3]"Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine:pyruvate aminotransferase of rat liver."
Oda T., Funai T., Ichiyama A.
J. Biol. Chem. 265:7513-7519(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE INITIATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06357 mRNA. Translation: CAA29656.1.
D13667 Genomic DNA. Translation: BAA02838.1.
M35270 mRNA. Translation: AAA42169.1.
PIRXNRTSP. S00164.
RefSeqNP_001263635.1. NM_001276706.1.
NP_085914.2. NM_030656.2.
UniGeneRn.9931.

3D structure databases

ProteinModelPortalP09139.
SMRP09139. Positions 26-411.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP09139.
PRIDEP09139.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000029127; ENSRNOP00000030340; ENSRNOG00000023856. [P09139-1]
GeneID24792.
KEGGrno:24792.
UCSCRGD:2073. rat. [P09139-1]

Organism-specific databases

CTD189.
RGD2073. Agxt.

Phylogenomic databases

eggNOGCOG0075.
GeneTreeENSGT00390000006648.
HOGENOMHOG000171815.
HOVERGENHBG006907.
InParanoidP09139.
KOK00830.
OMAAYSCSQK.
OrthoDBEOG7CZK6B.
PhylomeDBP09139.
TreeFamTF313234.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13151.
SABIO-RKP09139.

Gene expression databases

ArrayExpressP09139.
GenevestigatorP09139.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR000192. Aminotrans_V/Cys_dSase.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFPIRSF000524. SPT. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
PROSITEPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604430.
PROP09139.

Entry information

Entry nameSPYA_RAT
AccessionPrimary (citable) accession number: P09139
Secondary accession number(s): Q9R2C7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families