Reviewed,
UniProtKB/Swiss-Prot P09139 (SPYA_RAT)
Last modified
January 19, 2010.
Version 95.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine--pyruvate aminotransferase, mitochondrial Short name=SPT EC=2.6.1.51 Alternative name(s): Alanine--glyoxylate aminotransferase Short name=AGT EC=2.6.1.44 | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 414 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes). |
| Catalytic activity | L-serine + pyruvate = 3-hydroxypyruvate + L-alanine. L-alanine + glyoxylate = pyruvate + glycine. |
| Cofactor | Pyridoxal phosphate. |
| Subcellular location | Mitochondrion matrix. Peroxisome. Note: Induced on glucagon administration. Not affected by glucagon. |
| Sequence similarities | Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Mitochondrion Peroxisome |
| Coding sequence diversity | Alternative initiation |
| Domain | Transit peptide |
| Ligand | Pyridoxal phosphate |
| Molecular function | Aminotransferase Transferase |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | glycine biosynthetic process, by transamination of glyoxylate Inferred from direct assay. Source: RGD pyruvate biosynthetic processInferred from direct assay. Source: RGD response to cAMPInferred from direct assay. Source: RGD response to glucocorticoid stimulusInferred from direct assay. Source: RGD |
| Cellular component | mitochondrial matrix Inferred from direct assay. Source: HGNC peroxisomeInferred from direct assay. Source: HGNC |
| Molecular function | alanine-glyoxylate transaminase activity Inferred from direct assay. Source: RGD amino acid bindingInferred from direct assay. Source: RGD serine-pyruvate transaminase activityInferred from direct assay. Source: RGD |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative initiation. [Align] [Select] | ||||||
| Isoform Mitochondrial (identifier: P09139-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Peroxisomal (identifier: P09139-2) The sequence of this isoform differs from the canonical sequence as follows: 1-22: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 23 | 23 | Mitochondrion | ||||||
| Chain | 24 – 414 | 391 | Serine--pyruvate aminotransferase, mitochondrial | PRO_0000001290 | |||||
Regions | |||||||||
| Motif | 412 – 414 | 3 | Microbody targeting signal By similarity | ||||||
Sites | |||||||||
| Binding site | 382 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 231 | 1 | N6-(pyridoxal phosphate)lysine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 22 | 22 | Missing in isoform Peroxisomal. | VSP_018645 | |||||
Sequences
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References
| [1] | "Nucleotide sequence of the cDNA encoding the precursor for mitochondrial serine:pyruvate aminotransferase of rat liver." Oda T., Miyajima H., Suzuki Y., Ichiyama A. Eur. J. Biochem. 168:537-542(1987) [PubMed: 2822418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver. |
| [2] | "Characterization and sequence analysis of rat serine:pyruvate/alanine:glyoxylate aminotransferase gene." Oda T., Nishiyama K., Ichiyama A. Genomics 17:59-65(1993) [PubMed: 8406472] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-141. Strain: Wistar. |
| [3] | "Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine:pyruvate aminotransferase of rat liver." Oda T., Funai T., Ichiyama A. J. Biol. Chem. 265:7513-7519(1990) [PubMed: 2332438] [Abstract] Cited for: ALTERNATIVE INITIATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X06357 mRNA. Translation: CAA29656.1. D13667 Genomic DNA. Translation: BAA02838.1. M35270 mRNA. Translation: AAA42169.1. |
| IPI | IPI00211959. IPI00760135. |
| PIR | XNRTSP. S00164. |
| RefSeq | NP_085914.1. |
| UniGene | Rn.9931 |
3D structure databases | |
| SMR | P09139. Positions 26-411. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P09139. |
Proteomic databases | |
| PRIDE | P09139. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000029127; ENSRNOP00000030340; ENSRNOG00000023856; Rattus norvegicus. [Genome view] |
| GeneID | 24792. |
| KEGG | rno:24792. |
| UCSC | M35270. rat. |
Organism-specific databases | |
| CTD | 24792. |
| RGD | 2073. Agxt. |
Phylogenomic databases | |
| eggNOG | roNOG07527. |
| HOVERGEN | P09139. |
| InParanoid | P09139. |
| OMA | MRQNVER. |
| OrthoDB | EOG9BK7QF. |
| PhylomeDB | P09139. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-13151. |
| BRENDA | 2.6.1.44. 248. 2.6.1.51. 248. |
Gene expression databases | |
| ArrayExpress | P09139. |
| Genevestigator | P09139. |
| GermOnline | ENSRNOG00000023856. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view] |
| Gene3D | G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. |
| Pfam | PF00266. Aminotran_5. 1 hit. [Graphical view] |
| PROSITE | PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 604430. |
Entry information
| Entry name | SPYA_RAT | ||||||||
| Accession | Primary (citable) accession number: P09139 Secondary accession number(s): Q9R2C7 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

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