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P09139

- SPYA_RAT

UniProt

P09139 - SPYA_RAT

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Protein

Serine--pyruvate aminotransferase, mitochondrial

Gene
Agxt, Agt1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).

Catalytic activityi

L-serine + pyruvate = 3-hydroxypyruvate + L-alanine.
L-alanine + glyoxylate = pyruvate + glycine.

Cofactori

Pyridoxal phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei382 – 3821Substrate By similarity

GO - Molecular functioni

  1. alanine-glyoxylate transaminase activity Source: RGD
  2. amino acid binding Source: RGD
  3. pyridoxal phosphate binding Source: Ensembl
  4. serine-pyruvate transaminase activity Source: RGD

GO - Biological processi

  1. glycine biosynthetic process, by transamination of glyoxylate Source: RGD
  2. glyoxylate catabolic process Source: Ensembl
  3. L-alanine catabolic process Source: Ensembl
  4. L-cysteine catabolic process Source: Ensembl
  5. oxalic acid secretion Source: Ensembl
  6. protein targeting to peroxisome Source: Ensembl
  7. pyruvate biosynthetic process Source: RGD
  8. response to cAMP Source: RGD
  9. response to glucocorticoid Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13151.
SABIO-RKP09139.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--pyruvate aminotransferase, mitochondrial (EC:2.6.1.51)
Short name:
SPT
Alternative name(s):
Alanine--glyoxylate aminotransferase (EC:2.6.1.44)
Short name:
AGT
Gene namesi
Name:Agxt
Synonyms:Agt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi2073. Agxt.

Subcellular locationi

Mitochondrion matrix. Peroxisome
Note: Induced on glucagon administration. Not affected by glucagon.

GO - Cellular componenti

  1. mitochondrial matrix Source: HGNC
  2. mitochondrion Source: HGNC
  3. peroxisomal matrix Source: Ensembl
  4. peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2323MitochondrionAdd
BLAST
Chaini24 – 414391Serine--pyruvate aminotransferase, mitochondrialPRO_0000001290Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei231 – 2311N6-(pyridoxal phosphate)lysine By similarity
Modified residuei247 – 2471N6-acetyllysine; alternate By similarity
Modified residuei247 – 2471N6-succinyllysine; alternate By similarity
Modified residuei256 – 2561N6-acetyllysine By similarity
Modified residuei334 – 3341N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP09139.
PRIDEiP09139.

Expressioni

Gene expression databases

ArrayExpressiP09139.
GenevestigatoriP09139.

Structurei

3D structure databases

ProteinModelPortaliP09139.
SMRiP09139. Positions 26-411.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi412 – 4143Microbody targeting signal By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0075.
GeneTreeiENSGT00390000006648.
HOGENOMiHOG000171815.
HOVERGENiHBG006907.
InParanoidiP09139.
KOiK00830.
OMAiIAGTEHQ.
OrthoDBiEOG7CZK6B.
PhylomeDBiP09139.
TreeFamiTF313234.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V/Cys_dSase.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF000524. SPT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Mitochondrial (identifier: P09139-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MFRMLAKASV TLGSRAASWV RNMGSHQLLV PPPEALSKPL SIPKRLLLGP    50
GPSNLAPRVL AAGSLRMIGH MQKEMFQIMD EIKQGIQYVF QTRNPLTLVV 100
SGSGHCAMET ALFNLLEPGD SFLVGTNGIW GIRAAEIAER IGARVHQMIK 150
KPGEHYTLQE VEEGLAQHKP VLLFLTHGES STGVLQPLDG FGELCHRYQC 200
LLLVDSVASL GGVPIYMDQQ GIDILYSGSQ KVLNAPPGIS LISFNDKAKS 250
KVYSRKTKPV SFYTDITYLS KLWGCEGKTR VIHHTLPVIS LYCLRESLAL 300
ISEQGLENSW RRHREATAHL HKCLRELGLK FFVKDPEIRL PTITTVTVPA 350
GYNWRDIVSY VLDHFNIEIS GGLGPSEDKV LRIGLLGYNA TTENADRVAE 400
ALREALQHCP KNKL 414
Length:414
Mass (Da):45,834
Last modified:July 1, 1989 - v1
Checksum:i3FBD66ED36C3D9EF
GO
Isoform Peroxisomal (identifier: P09139-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:392
Mass (Da):43,399
Checksum:i5A42F40FD95B6E3E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222Missing in isoform Peroxisomal. VSP_018645Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06357 mRNA. Translation: CAA29656.1.
D13667 Genomic DNA. Translation: BAA02838.1.
M35270 mRNA. Translation: AAA42169.1.
PIRiS00164. XNRTSP.
RefSeqiNP_001263635.1. NM_001276706.1. [P09139-2]
NP_085914.2. NM_030656.2. [P09139-1]
UniGeneiRn.9931.

Genome annotation databases

EnsembliENSRNOT00000029127; ENSRNOP00000030340; ENSRNOG00000023856. [P09139-1]
GeneIDi24792.
KEGGirno:24792.
UCSCiRGD:2073. rat. [P09139-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06357 mRNA. Translation: CAA29656.1 .
D13667 Genomic DNA. Translation: BAA02838.1 .
M35270 mRNA. Translation: AAA42169.1 .
PIRi S00164. XNRTSP.
RefSeqi NP_001263635.1. NM_001276706.1. [P09139-2 ]
NP_085914.2. NM_030656.2. [P09139-1 ]
UniGenei Rn.9931.

3D structure databases

ProteinModelPortali P09139.
SMRi P09139. Positions 26-411.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P09139.
PRIDEi P09139.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000029127 ; ENSRNOP00000030340 ; ENSRNOG00000023856 . [P09139-1 ]
GeneIDi 24792.
KEGGi rno:24792.
UCSCi RGD:2073. rat. [P09139-1 ]

Organism-specific databases

CTDi 189.
RGDi 2073. Agxt.

Phylogenomic databases

eggNOGi COG0075.
GeneTreei ENSGT00390000006648.
HOGENOMi HOG000171815.
HOVERGENi HBG006907.
InParanoidi P09139.
KOi K00830.
OMAi IAGTEHQ.
OrthoDBi EOG7CZK6B.
PhylomeDBi P09139.
TreeFami TF313234.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13151.
SABIO-RK P09139.

Miscellaneous databases

NextBioi 604430.
PROi P09139.

Gene expression databases

ArrayExpressi P09139.
Genevestigatori P09139.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR000192. Aminotrans_V/Cys_dSase.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR024169. SP_NH2Trfase/AEP_transaminase.
[Graphical view ]
Pfami PF00266. Aminotran_5. 1 hit.
[Graphical view ]
PIRSFi PIRSF000524. SPT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the cDNA encoding the precursor for mitochondrial serine:pyruvate aminotransferase of rat liver."
    Oda T., Miyajima H., Suzuki Y., Ichiyama A.
    Eur. J. Biochem. 168:537-542(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  2. "Characterization and sequence analysis of rat serine:pyruvate/alanine:glyoxylate aminotransferase gene."
    Oda T., Nishiyama K., Ichiyama A.
    Genomics 17:59-65(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-141.
    Strain: Wistar.
  3. "Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine:pyruvate aminotransferase of rat liver."
    Oda T., Funai T., Ichiyama A.
    J. Biol. Chem. 265:7513-7519(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE INITIATION.

Entry informationi

Entry nameiSPYA_RAT
AccessioniPrimary (citable) accession number: P09139
Secondary accession number(s): Q9R2C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 14, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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