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P09139

- SPYA_RAT

UniProt

P09139 - SPYA_RAT

Protein

Serine--pyruvate aminotransferase, mitochondrial

Gene

Agxt

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).

    Catalytic activityi

    L-serine + pyruvate = 3-hydroxypyruvate + L-alanine.
    L-alanine + glyoxylate = pyruvate + glycine.

    Cofactori

    Pyridoxal phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei382 – 3821SubstrateBy similarity

    GO - Molecular functioni

    1. alanine-glyoxylate transaminase activity Source: RGD
    2. amino acid binding Source: RGD
    3. pyridoxal phosphate binding Source: Ensembl
    4. serine-pyruvate transaminase activity Source: RGD

    GO - Biological processi

    1. glycine biosynthetic process, by transamination of glyoxylate Source: RGD
    2. glyoxylate catabolic process Source: Ensembl
    3. L-alanine catabolic process Source: Ensembl
    4. L-cysteine catabolic process Source: Ensembl
    5. oxalic acid secretion Source: Ensembl
    6. protein targeting to peroxisome Source: Ensembl
    7. pyruvate biosynthetic process Source: RGD
    8. response to cAMP Source: RGD
    9. response to glucocorticoid Source: RGD

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13151.
    SABIO-RKP09139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine--pyruvate aminotransferase, mitochondrial (EC:2.6.1.51)
    Short name:
    SPT
    Alternative name(s):
    Alanine--glyoxylate aminotransferase (EC:2.6.1.44)
    Short name:
    AGT
    Gene namesi
    Name:Agxt
    Synonyms:Agt1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi2073. Agxt.

    Subcellular locationi

    Mitochondrion matrix. Peroxisome
    Note: Induced on glucagon administration. Not affected by glucagon.

    GO - Cellular componenti

    1. mitochondrial matrix Source: HGNC
    2. mitochondrion Source: HGNC
    3. peroxisomal matrix Source: Ensembl
    4. peroxisome Source: HGNC

    Keywords - Cellular componenti

    Mitochondrion, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2323MitochondrionAdd
    BLAST
    Chaini24 – 414391Serine--pyruvate aminotransferase, mitochondrialPRO_0000001290Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei231 – 2311N6-(pyridoxal phosphate)lysineBy similarity
    Modified residuei247 – 2471N6-acetyllysine; alternateBy similarity
    Modified residuei247 – 2471N6-succinyllysine; alternateBy similarity
    Modified residuei256 – 2561N6-acetyllysineBy similarity
    Modified residuei334 – 3341N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP09139.
    PRIDEiP09139.

    Expressioni

    Gene expression databases

    ArrayExpressiP09139.
    GenevestigatoriP09139.

    Structurei

    3D structure databases

    ProteinModelPortaliP09139.
    SMRiP09139. Positions 26-411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi412 – 4143Microbody targeting signalBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0075.
    GeneTreeiENSGT00390000006648.
    HOGENOMiHOG000171815.
    HOVERGENiHBG006907.
    InParanoidiP09139.
    KOiK00830.
    OMAiIAGTEHQ.
    OrthoDBiEOG7CZK6B.
    PhylomeDBiP09139.
    TreeFamiTF313234.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR024169. SP_NH2Trfase/AEP_transaminase.
    [Graphical view]
    PfamiPF00266. Aminotran_5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000524. SPT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform Mitochondrial (identifier: P09139-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFRMLAKASV TLGSRAASWV RNMGSHQLLV PPPEALSKPL SIPKRLLLGP    50
    GPSNLAPRVL AAGSLRMIGH MQKEMFQIMD EIKQGIQYVF QTRNPLTLVV 100
    SGSGHCAMET ALFNLLEPGD SFLVGTNGIW GIRAAEIAER IGARVHQMIK 150
    KPGEHYTLQE VEEGLAQHKP VLLFLTHGES STGVLQPLDG FGELCHRYQC 200
    LLLVDSVASL GGVPIYMDQQ GIDILYSGSQ KVLNAPPGIS LISFNDKAKS 250
    KVYSRKTKPV SFYTDITYLS KLWGCEGKTR VIHHTLPVIS LYCLRESLAL 300
    ISEQGLENSW RRHREATAHL HKCLRELGLK FFVKDPEIRL PTITTVTVPA 350
    GYNWRDIVSY VLDHFNIEIS GGLGPSEDKV LRIGLLGYNA TTENADRVAE 400
    ALREALQHCP KNKL 414
    Length:414
    Mass (Da):45,834
    Last modified:July 1, 1989 - v1
    Checksum:i3FBD66ED36C3D9EF
    GO
    Isoform Peroxisomal (identifier: P09139-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-22: Missing.

    Show »
    Length:392
    Mass (Da):43,399
    Checksum:i5A42F40FD95B6E3E
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2222Missing in isoform Peroxisomal. CuratedVSP_018645Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06357 mRNA. Translation: CAA29656.1.
    D13667 Genomic DNA. Translation: BAA02838.1.
    M35270 mRNA. Translation: AAA42169.1.
    PIRiS00164. XNRTSP.
    RefSeqiNP_001263635.1. NM_001276706.1. [P09139-2]
    NP_085914.2. NM_030656.2. [P09139-1]
    UniGeneiRn.9931.

    Genome annotation databases

    EnsembliENSRNOT00000029127; ENSRNOP00000030340; ENSRNOG00000023856. [P09139-1]
    GeneIDi24792.
    KEGGirno:24792.
    UCSCiRGD:2073. rat. [P09139-1]

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06357 mRNA. Translation: CAA29656.1 .
    D13667 Genomic DNA. Translation: BAA02838.1 .
    M35270 mRNA. Translation: AAA42169.1 .
    PIRi S00164. XNRTSP.
    RefSeqi NP_001263635.1. NM_001276706.1. [P09139-2 ]
    NP_085914.2. NM_030656.2. [P09139-1 ]
    UniGenei Rn.9931.

    3D structure databases

    ProteinModelPortali P09139.
    SMRi P09139. Positions 26-411.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P09139.
    PRIDEi P09139.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000029127 ; ENSRNOP00000030340 ; ENSRNOG00000023856 . [P09139-1 ]
    GeneIDi 24792.
    KEGGi rno:24792.
    UCSCi RGD:2073. rat. [P09139-1 ]

    Organism-specific databases

    CTDi 189.
    RGDi 2073. Agxt.

    Phylogenomic databases

    eggNOGi COG0075.
    GeneTreei ENSGT00390000006648.
    HOGENOMi HOG000171815.
    HOVERGENi HBG006907.
    InParanoidi P09139.
    KOi K00830.
    OMAi IAGTEHQ.
    OrthoDBi EOG7CZK6B.
    PhylomeDBi P09139.
    TreeFami TF313234.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-13151.
    SABIO-RK P09139.

    Miscellaneous databases

    NextBioi 604430.
    PROi P09139.

    Gene expression databases

    ArrayExpressi P09139.
    Genevestigatori P09139.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR024169. SP_NH2Trfase/AEP_transaminase.
    [Graphical view ]
    Pfami PF00266. Aminotran_5. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000524. SPT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the cDNA encoding the precursor for mitochondrial serine:pyruvate aminotransferase of rat liver."
      Oda T., Miyajima H., Suzuki Y., Ichiyama A.
      Eur. J. Biochem. 168:537-542(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Liver.
    2. "Characterization and sequence analysis of rat serine:pyruvate/alanine:glyoxylate aminotransferase gene."
      Oda T., Nishiyama K., Ichiyama A.
      Genomics 17:59-65(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-141.
      Strain: Wistar.
    3. "Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine:pyruvate aminotransferase of rat liver."
      Oda T., Funai T., Ichiyama A.
      J. Biol. Chem. 265:7513-7519(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE INITIATION.

    Entry informationi

    Entry nameiSPYA_RAT
    AccessioniPrimary (citable) accession number: P09139
    Secondary accession number(s): Q9R2C7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3