P09132 (SRP19_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal recognition particle 19 kDa protein
Short name=SRP19

Gene names

Name:

SRP19

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Signal-recognition-particle assembly, binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.
Subunit structure	Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the SRP19 family.

Ontologies

Keywords
Cellular component	Cytoplasm Signal recognition particle
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: Reactome response to drug Inferred from direct assay PubMed 18089836. Source: UniProtKB translation Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome mitochondrion Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay PubMed 10618370. Source: MGI signal recognition particle, endoplasmic reticulum targeting Inferred from direct assay PubMed 18089836. Source: UniProtKB
Molecular_function	7S RNA binding Inferred from direct assay PubMed 17434535. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P09132-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P09132-2)

The sequence of this isoform differs from the canonical sequence as follows:
     101-144: RKSVMLYAAEMIPKLKTRTQKTGGADQSLQQGEGSKKGKGKKKK → HYTLSLTSGS

Note: No experimental confirmation available.

Isoform 3 (identifier: P09132-3)

The sequence of this isoform differs from the canonical sequence as follows:
     15-144: FICIYPAYLN...SKKGKGKKKK → LLKILQLQRF...ALYSSHHVSQ

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 144

144

Signal recognition particle 19 kDa protein

PRO_0000135197

Regions

Region

136 – 144

9

Basic region, potentially involved in RNA-binding

Natural variations

Alternative sequence

15 – 144

130

FICIY…GKKKK → LLKILQLQRFKMYVQQLDLT YFLRKIKCTLENGIVMSNTE AESGSSSNRKMGASALYSSH HVSQ in isoform 3.

VSP_044524

Alternative sequence

101 – 144

44

RKSVM…GKKKK → HYTLSLTSGS in isoform 2.

VSP_042540

Natural variant

4

1

A → T. Ref.6
Corresponds to variant rs17855423 [ dbSNP | Ensembl ].

VAR_027800

Secondary structure

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144

Helix Strand Turn

Details...

Helix

12 – 14

3

Beta strand

15 – 18

4

Helix

20 – 23

4

Turn

29 – 32

4

Turn

37 – 39

3

Beta strand

41 – 43

3

Helix

46 – 55

10

Beta strand

60 – 63

4

Helix

76 – 78

3

Beta strand

81 – 84

4

Beta strand

93 – 95

3

Helix

101 – 111

11

Helix

112 – 114

3

Helix

116 – 119

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 3, 2006. Version 3. Checksum: E25F661972338CAE	FASTA	144	16,156
	10 20 30 40 50 60 MACAAARSPA DQDRFICIYP AYLNNKKTIA EGRRIPISKA VENPTATEIQ DVCSAVGLNV 70 80 90 100 110 120 FLEKNKMYSR EWNRDVQYRG RVRVQLKQED GSLCLVQFPS RKSVMLYAAE MIPKLKTRTQ 130 140 KTGGADQSLQ QGEGSKKGKG KKKK
	Isoform 2 [UniParc]. Checksum: F199656A14C743E1	FASTA	110	12,400
	10 20 30 40 50 60 MACAAARSPA DQDRFICIYP AYLNNKKTIA EGRRIPISKA VENPTATEIQ DVCSAVGLNV 70 80 90 100 110 FLEKNKMYSR EWNRDVQYRG RVRVQLKQED GSLCLVQFPS HYTLSLTSGS
	Isoform 3 [UniParc]. Checksum: 55C1707F8C5C2AE0	FASTA	78	8,739
	10 20 30 40 50 60 MACAAARSPA DQDRLLKILQ LQRFKMYVQQ LDLTYFLRKI KCTLENGIVM SNTEAESGSS 70 SNRKMGASAL YSSHHVSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a cDNA clone encoding the 19 kDa protein of signal recognition particle (SRP): expression and binding to 7SL RNA." Lingelbach K., Zwieb C., Webb J.R., Marshallsaz C., Hoben P., Walter P., Dobberstein B. Nucleic Acids Res. 16:9431-9442(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Gene expression in human erythroid precursor cells." Gubin A.N., Lee Y.T., Bouffard G.G., Miller J.L. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Erythroblast.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT THR-4. Tissue: Brain and Skin.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Crystal structure of an early protein-RNA assembly complex of the signal recognition particle." Wild K., Sinning I., Cusack S. Science 294:598-601(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 1-128.
[9]	"Induced structural changes of 7SL RNA during the assembly of human signal recognition particle." Kuglstatter A., Oubridge C., Nagai K. Nat. Struct. Biol. 9:740-744(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 14-120 IN COMPLEX WITH SRP54 AND 7SL RNA.
[10]	"Structural insights into the assembly of the human and archaeal signal recognition particles." Wild K., Bange G., Bozkurt G., Segnitz B., Hendricks A., Sinning I. Acta Crystallogr. D 66:295-303(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF 1-120 IN COMPLEX WITH 7SL RNA.
+	Additional computationally mapped references.

Web resources

Wikipedia

Signal recognition particle entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X12791 mRNA. Translation: CAA31280.1.
BU661702 mRNA. No translation available.
AK311803 mRNA. Translation: BAG34746.1.
AC008536 Genomic DNA. No translation available.
AC008575 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW48999.1.
CH471086 Genomic DNA. Translation: EAW49000.1.
BC010947 mRNA. Translation: AAH10947.1.
BC017830 mRNA. Translation: AAH17830.1.

IPI

IPI00295889.
IPI00807414.
IPI00967461.

PIR

S01700.

RefSeq

NP_001191122.1. NM_001204193.1.
NP_001191123.1. NM_001204194.1.
NP_001191125.1. NM_001204196.1.
NP_003126.1. NM_003135.2.

UniGene

Hs.637001.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JID	X-ray	1.80	A	1-120	[»]
1MFQ	X-ray	3.10	B	14-120	[»]
1RY1	electron microscopy	12.00	B	14-120	[»]
2J37	electron microscopy	8.00	B	14-120	[»]
3KTV	X-ray	3.80	B/D	1-120	[»]

ProteinModelPortal

P09132.

ModBase

Search...

Protein-protein interaction databases

IntAct

P09132. 2 interactions.

MINT

MINT-247583.

STRING

9606.ENSP00000282999.

Protein family/group databases

TCDB

3.A.5.9.1. general secretory pathway (Sec) family.

PTM databases

PhosphoSite

P09132.

Polymorphism databases

DMDM

115502457.

Proteomic databases

PaxDb

P09132.

PRIDE

P09132.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000282999; ENSP00000282999; ENSG00000153037.
ENST00000505459; ENSP00000424870; ENSG00000153037.
ENST00000515463; ENSP00000425562; ENSG00000153037.

GeneID

6728.

KEGG

hsa:6728.

UCSC

uc003kqc.3. human.

Organism-specific databases

CTD

6728.

GeneCards

GC05P112196.

HGNC

HGNC:11300. SRP19.

HPA

HPA029272.

MIM

182175. gene.

neXtProt

NX_P09132.

PharmGKB

PA36124.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG265516.

HOGENOM

HOG000237221.

HOVERGEN

HBG059463.

InParanoid

P09132.

KO

K03105.

OMA

YVAEMIP.

OrthoDB

EOG48D0WP.

PhylomeDB

P09132.

Enzyme and pathway databases

Reactome

REACT_17015. Metabolism of proteins.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P09132.

Bgee

P09132.

CleanEx

HS_SRP19.

Genevestigator

P09132.

GermOnline

ENSG00000153037. Homo sapiens.

Family and domain databases

Gene3D

3.30.56.30. 1 hit.

InterPro

IPR002778. Signal_recog_particle_SRP19.
[Graphical view]

PANTHER

PTHR17453. PTHR17453. 1 hit.

Pfam

PF01922. SRP19. 1 hit.
[Graphical view]

SUPFAM

SSF69695. Signal_recog_particle_SRP19. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

SRP19. human.

EvolutionaryTrace

P09132.

GenomeRNAi

6728.

NextBio

26244.

SOURCE

Search...

Entry information

Entry name

SRP19_HUMAN

Accession

Primary (citable) accession number: P09132
Secondary accession number(s): B2R4E9 , D6RCQ5, Q05D77, Q96FG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 139 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.