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Protein

Putative uroporphyrinogen-III C-methyltransferase

Gene

hemX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Putative uroporphyrinogen-III C-methyltransferase (hemX)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:HEMX-MONOMER.
ECOL316407:JW3775-MONOMER.
MetaCyc:HEMX-MONOMER.
UniPathwayiUPA00148; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen-III methylase
Alternative name(s):
ORF X
Gene namesi
Name:hemX
Ordered Locus Names:b3803, JW3775
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10433. hemX.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352551 – 393Putative uroporphyrinogen-III C-methyltransferaseAdd BLAST393

Proteomic databases

EPDiP09127.
PaxDbiP09127.
PRIDEiP09127.

Interactioni

Subunit structurei

Homooligomer, possibly a homopentamer.1 Publication

Protein-protein interaction databases

BioGridi4259707. 141 interactors.
DIPiDIP-9888N.
IntActiP09127. 3 interactors.
STRINGi511145.b3803.

Structurei

3D structure databases

ProteinModelPortaliP09127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ECF. Bacteria.
COG2959. LUCA.
HOGENOMiHOG000270899.
InParanoidiP09127.
KOiK02496.
OMAiSQQSITM.
PhylomeDBiP09127.

Family and domain databases

InterProiIPR007470. HemX.
[Graphical view]
PfamiPF04375. HemX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09127-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQEKTSAV VEETREAVDT TSQPVATEKK SKNNTALILS AVAIAIALAA
60 70 80 90 100
GIGLYGWGKQ QAVNQTATSD ALANQLTALQ KAQESQKAEL EGIIKQQAAQ
110 120 130 140 150
LKQANRQQET LAKQLDEVQQ KVATISGSDA KTWLLAQADF LVKLAGRKLW
160 170 180 190 200
SDQDVTTAAA LLKSADASLA DMNDPSLITV RRAITDDIAS LSAVSQVDYD
210 220 230 240 250
GIILKLNQLS NQVDNLRLAD NDSDGSPMDS DGEELSSSIS EWRINLQKSW
260 270 280 290 300
QNFMDNFITI RRRDDTAVPL LAPNQDIYLR ENIRSRLLVA AQAVPRHQEE
310 320 330 340 350
TYRQALENVS TWVRAYYDTD DATTKAFLDE VDQLSQQNIS MDLPETLQSQ
360 370 380 390
AMLEKLMQTR VRNLLAQPAA GTTEAKPAPA PQADTPAAAP QGE
Length:393
Mass (Da):42,963
Last modified:July 1, 1989 - v1
Checksum:i9D272C6401D0E354
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13406 Genomic DNA. Translation: CAA31772.1.
X12614 Genomic DNA. Translation: CAA31134.1.
M87049 Genomic DNA. Translation: AAA67599.1.
U00096 Genomic DNA. Translation: AAC76806.1.
AP009048 Genomic DNA. Translation: BAE77498.1.
PIRiS02185.
RefSeqiNP_418247.1. NC_000913.3.
WP_000138997.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76806; AAC76806; b3803.
BAE77498; BAE77498; BAE77498.
GeneIDi948446.
KEGGiecj:JW3775.
eco:b3803.
PATRICi32123105. VBIEscCol129921_3918.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13406 Genomic DNA. Translation: CAA31772.1.
X12614 Genomic DNA. Translation: CAA31134.1.
M87049 Genomic DNA. Translation: AAA67599.1.
U00096 Genomic DNA. Translation: AAC76806.1.
AP009048 Genomic DNA. Translation: BAE77498.1.
PIRiS02185.
RefSeqiNP_418247.1. NC_000913.3.
WP_000138997.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP09127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259707. 141 interactors.
DIPiDIP-9888N.
IntActiP09127. 3 interactors.
STRINGi511145.b3803.

Proteomic databases

EPDiP09127.
PaxDbiP09127.
PRIDEiP09127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76806; AAC76806; b3803.
BAE77498; BAE77498; BAE77498.
GeneIDi948446.
KEGGiecj:JW3775.
eco:b3803.
PATRICi32123105. VBIEscCol129921_3918.

Organism-specific databases

EchoBASEiEB0428.
EcoGeneiEG10433. hemX.

Phylogenomic databases

eggNOGiENOG4105ECF. Bacteria.
COG2959. LUCA.
HOGENOMiHOG000270899.
InParanoidiP09127.
KOiK02496.
OMAiSQQSITM.
PhylomeDBiP09127.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
BioCyciEcoCyc:HEMX-MONOMER.
ECOL316407:JW3775-MONOMER.
MetaCyc:HEMX-MONOMER.

Miscellaneous databases

PROiP09127.

Family and domain databases

InterProiIPR007470. HemX.
[Graphical view]
PfamiPF04375. HemX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEMX_ECOLI
AccessioniPrimary (citable) accession number: P09127
Secondary accession number(s): Q2M8A8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.