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P09127

- HEMX_ECOLI

UniProt

P09127 - HEMX_ECOLI

Protein

Putative uroporphyrinogen-III C-methyltransferase

Gene

hemX

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
    S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

    Pathwayi

    GO - Molecular functioni

    1. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
    2. porphyrin-containing compound biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Porphyrin biosynthesis

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:HEMX-MONOMER.
    ECOL316407:JW3775-MONOMER.
    UniPathwayiUPA00148; UER00211.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
    Short name:
    Urogen-III methylase
    Alternative name(s):
    ORF X
    Gene namesi
    Name:hemX
    Ordered Locus Names:b3803, JW3775
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10433. hemX.

    Subcellular locationi

    Cell inner membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 393393Putative uroporphyrinogen-III C-methyltransferasePRO_0000135255Add
    BLAST

    Proteomic databases

    PaxDbiP09127.
    PRIDEiP09127.

    Expressioni

    Gene expression databases

    GenevestigatoriP09127.

    Interactioni

    Subunit structurei

    Homooligomer, possibly a homopentamer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    dapEP0AED71EBI-1128342,EBI-1129009

    Protein-protein interaction databases

    DIPiDIP-9888N.
    IntActiP09127. 3 interactions.
    STRINGi511145.b3803.

    Structurei

    3D structure databases

    ProteinModelPortaliP09127.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei37 – 5721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2959.
    HOGENOMiHOG000270899.
    KOiK02496.
    OMAiTRVRNLM.
    OrthoDBiEOG6QK4QM.
    PhylomeDBiP09127.

    Family and domain databases

    InterProiIPR007470. HemX.
    [Graphical view]
    PfamiPF04375. HemX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09127-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEQEKTSAV VEETREAVDT TSQPVATEKK SKNNTALILS AVAIAIALAA    50
    GIGLYGWGKQ QAVNQTATSD ALANQLTALQ KAQESQKAEL EGIIKQQAAQ 100
    LKQANRQQET LAKQLDEVQQ KVATISGSDA KTWLLAQADF LVKLAGRKLW 150
    SDQDVTTAAA LLKSADASLA DMNDPSLITV RRAITDDIAS LSAVSQVDYD 200
    GIILKLNQLS NQVDNLRLAD NDSDGSPMDS DGEELSSSIS EWRINLQKSW 250
    QNFMDNFITI RRRDDTAVPL LAPNQDIYLR ENIRSRLLVA AQAVPRHQEE 300
    TYRQALENVS TWVRAYYDTD DATTKAFLDE VDQLSQQNIS MDLPETLQSQ 350
    AMLEKLMQTR VRNLLAQPAA GTTEAKPAPA PQADTPAAAP QGE 393
    Length:393
    Mass (Da):42,963
    Last modified:July 1, 1989 - v1
    Checksum:i9D272C6401D0E354
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13406 Genomic DNA. Translation: CAA31772.1.
    X12614 Genomic DNA. Translation: CAA31134.1.
    M87049 Genomic DNA. Translation: AAA67599.1.
    U00096 Genomic DNA. Translation: AAC76806.1.
    AP009048 Genomic DNA. Translation: BAE77498.1.
    PIRiS02185.
    RefSeqiNP_418247.1. NC_000913.3.
    YP_491639.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76806; AAC76806; b3803.
    BAE77498; BAE77498; BAE77498.
    GeneIDi12930713.
    948446.
    KEGGiecj:Y75_p3375.
    eco:b3803.
    PATRICi32123105. VBIEscCol129921_3918.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13406 Genomic DNA. Translation: CAA31772.1 .
    X12614 Genomic DNA. Translation: CAA31134.1 .
    M87049 Genomic DNA. Translation: AAA67599.1 .
    U00096 Genomic DNA. Translation: AAC76806.1 .
    AP009048 Genomic DNA. Translation: BAE77498.1 .
    PIRi S02185.
    RefSeqi NP_418247.1. NC_000913.3.
    YP_491639.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P09127.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9888N.
    IntActi P09127. 3 interactions.
    STRINGi 511145.b3803.

    Proteomic databases

    PaxDbi P09127.
    PRIDEi P09127.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76806 ; AAC76806 ; b3803 .
    BAE77498 ; BAE77498 ; BAE77498 .
    GeneIDi 12930713.
    948446.
    KEGGi ecj:Y75_p3375.
    eco:b3803.
    PATRICi 32123105. VBIEscCol129921_3918.

    Organism-specific databases

    EchoBASEi EB0428.
    EcoGenei EG10433. hemX.

    Phylogenomic databases

    eggNOGi COG2959.
    HOGENOMi HOG000270899.
    KOi K02496.
    OMAi TRVRNLM.
    OrthoDBi EOG6QK4QM.
    PhylomeDBi P09127.

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00211 .
    BioCyci EcoCyc:HEMX-MONOMER.
    ECOL316407:JW3775-MONOMER.

    Miscellaneous databases

    PROi P09127.

    Gene expression databases

    Genevestigatori P09127.

    Family and domain databases

    InterProi IPR007470. HemX.
    [Graphical view ]
    Pfami PF04375. HemX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the hemX gene, the third member of the Uro operon of Escherichia coli K12."
      Sasarman A., Echelard Y., Letowski J., Tardif D., Drolet M.
      Nucleic Acids Res. 16:11835-11835(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "The sequence of hemC, hemD and two additional E. coli genes."
      Alefounder P.R., Abell C., Battersby A.R.
      Nucleic Acids Res. 16:9871-9871(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / CS520.
    3. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
      Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
      Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-12.
      Strain: K12 / EMG2.
    7. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    8. Cited for: SUBUNIT, SUBCELLULAR LOCATION.
      Strain: BL21-DE3.

    Entry informationi

    Entry nameiHEMX_ECOLI
    AccessioniPrimary (citable) accession number: P09127
    Secondary accession number(s): Q2M8A8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3