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P09127

- HEMX_ECOLI

UniProt

P09127 - HEMX_ECOLI

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Protein

Putative uroporphyrinogen-III C-methyltransferase

Gene

hemX

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Pathwayi

GO - Molecular functioni

  1. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
  2. porphyrin-containing compound biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:HEMX-MONOMER.
ECOL316407:JW3775-MONOMER.
UniPathwayiUPA00148; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen-III methylase
Alternative name(s):
ORF X
Gene namesi
Name:hemX
Ordered Locus Names:b3803, JW3775
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10433. hemX.

Subcellular locationi

Cell inner membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei37 – 5721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Putative uroporphyrinogen-III C-methyltransferasePRO_0000135255Add
BLAST

Proteomic databases

PaxDbiP09127.
PRIDEiP09127.

Expressioni

Gene expression databases

GenevestigatoriP09127.

Interactioni

Subunit structurei

Homooligomer, possibly a homopentamer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
dapEP0AED71EBI-1128342,EBI-1129009

Protein-protein interaction databases

DIPiDIP-9888N.
IntActiP09127. 3 interactions.
STRINGi511145.b3803.

Structurei

3D structure databases

ProteinModelPortaliP09127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2959.
HOGENOMiHOG000270899.
InParanoidiP09127.
KOiK02496.
OMAiTRVRNLM.
OrthoDBiEOG6QK4QM.
PhylomeDBiP09127.

Family and domain databases

InterProiIPR007470. HemX.
[Graphical view]
PfamiPF04375. HemX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09127-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEQEKTSAV VEETREAVDT TSQPVATEKK SKNNTALILS AVAIAIALAA
60 70 80 90 100
GIGLYGWGKQ QAVNQTATSD ALANQLTALQ KAQESQKAEL EGIIKQQAAQ
110 120 130 140 150
LKQANRQQET LAKQLDEVQQ KVATISGSDA KTWLLAQADF LVKLAGRKLW
160 170 180 190 200
SDQDVTTAAA LLKSADASLA DMNDPSLITV RRAITDDIAS LSAVSQVDYD
210 220 230 240 250
GIILKLNQLS NQVDNLRLAD NDSDGSPMDS DGEELSSSIS EWRINLQKSW
260 270 280 290 300
QNFMDNFITI RRRDDTAVPL LAPNQDIYLR ENIRSRLLVA AQAVPRHQEE
310 320 330 340 350
TYRQALENVS TWVRAYYDTD DATTKAFLDE VDQLSQQNIS MDLPETLQSQ
360 370 380 390
AMLEKLMQTR VRNLLAQPAA GTTEAKPAPA PQADTPAAAP QGE
Length:393
Mass (Da):42,963
Last modified:July 1, 1989 - v1
Checksum:i9D272C6401D0E354
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13406 Genomic DNA. Translation: CAA31772.1.
X12614 Genomic DNA. Translation: CAA31134.1.
M87049 Genomic DNA. Translation: AAA67599.1.
U00096 Genomic DNA. Translation: AAC76806.1.
AP009048 Genomic DNA. Translation: BAE77498.1.
PIRiS02185.
RefSeqiNP_418247.1. NC_000913.3.
YP_491639.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76806; AAC76806; b3803.
BAE77498; BAE77498; BAE77498.
GeneIDi12930713.
948446.
KEGGiecj:Y75_p3375.
eco:b3803.
PATRICi32123105. VBIEscCol129921_3918.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13406 Genomic DNA. Translation: CAA31772.1 .
X12614 Genomic DNA. Translation: CAA31134.1 .
M87049 Genomic DNA. Translation: AAA67599.1 .
U00096 Genomic DNA. Translation: AAC76806.1 .
AP009048 Genomic DNA. Translation: BAE77498.1 .
PIRi S02185.
RefSeqi NP_418247.1. NC_000913.3.
YP_491639.1. NC_007779.1.

3D structure databases

ProteinModelPortali P09127.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9888N.
IntActi P09127. 3 interactions.
STRINGi 511145.b3803.

Proteomic databases

PaxDbi P09127.
PRIDEi P09127.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76806 ; AAC76806 ; b3803 .
BAE77498 ; BAE77498 ; BAE77498 .
GeneIDi 12930713.
948446.
KEGGi ecj:Y75_p3375.
eco:b3803.
PATRICi 32123105. VBIEscCol129921_3918.

Organism-specific databases

EchoBASEi EB0428.
EcoGenei EG10433. hemX.

Phylogenomic databases

eggNOGi COG2959.
HOGENOMi HOG000270899.
InParanoidi P09127.
KOi K02496.
OMAi TRVRNLM.
OrthoDBi EOG6QK4QM.
PhylomeDBi P09127.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
BioCyci EcoCyc:HEMX-MONOMER.
ECOL316407:JW3775-MONOMER.

Miscellaneous databases

PROi P09127.

Gene expression databases

Genevestigatori P09127.

Family and domain databases

InterProi IPR007470. HemX.
[Graphical view ]
Pfami PF04375. HemX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the hemX gene, the third member of the Uro operon of Escherichia coli K12."
    Sasarman A., Echelard Y., Letowski J., Tardif D., Drolet M.
    Nucleic Acids Res. 16:11835-11835(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "The sequence of hemC, hemD and two additional E. coli genes."
    Alefounder P.R., Abell C., Battersby A.R.
    Nucleic Acids Res. 16:9871-9871(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / CS520.
  3. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12.
    Strain: K12 / EMG2.
  7. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  8. Cited for: SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BL21-DE3.

Entry informationi

Entry nameiHEMX_ECOLI
AccessioniPrimary (citable) accession number: P09127
Secondary accession number(s): Q2M8A8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3