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Protein

Putative uroporphyrinogen-III C-methyltransferase

Gene

hemX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG), Putative uroporphyrinogen-III C-methyltransferase (hemX)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processPorphyrin biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:HEMX-MONOMER.
MetaCyc:HEMX-MONOMER.
UniPathwayiUPA00148; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen-III methylase
Alternative name(s):
ORF X
Gene namesi
Name:hemX
Ordered Locus Names:b3803, JW3775
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10433. hemX.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352551 – 393Putative uroporphyrinogen-III C-methyltransferaseAdd BLAST393

Proteomic databases

PaxDbiP09127.
PRIDEiP09127.

Interactioni

Subunit structurei

Homooligomer, possibly a homopentamer.1 Publication

Protein-protein interaction databases

BioGridi4259707. 141 interactors.
DIPiDIP-9888N.
IntActiP09127. 3 interactors.
STRINGi316407.85676248.

Structurei

3D structure databases

ProteinModelPortaliP09127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ECF. Bacteria.
COG2959. LUCA.
HOGENOMiHOG000270899.
InParanoidiP09127.
KOiK02496.
PhylomeDBiP09127.

Family and domain databases

InterProiView protein in InterPro
IPR007470. HemX.
PfamiView protein in Pfam
PF04375. HemX. 1 hit.

Sequencei

Sequence statusi: Complete.

P09127-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQEKTSAV VEETREAVDT TSQPVATEKK SKNNTALILS AVAIAIALAA
60 70 80 90 100
GIGLYGWGKQ QAVNQTATSD ALANQLTALQ KAQESQKAEL EGIIKQQAAQ
110 120 130 140 150
LKQANRQQET LAKQLDEVQQ KVATISGSDA KTWLLAQADF LVKLAGRKLW
160 170 180 190 200
SDQDVTTAAA LLKSADASLA DMNDPSLITV RRAITDDIAS LSAVSQVDYD
210 220 230 240 250
GIILKLNQLS NQVDNLRLAD NDSDGSPMDS DGEELSSSIS EWRINLQKSW
260 270 280 290 300
QNFMDNFITI RRRDDTAVPL LAPNQDIYLR ENIRSRLLVA AQAVPRHQEE
310 320 330 340 350
TYRQALENVS TWVRAYYDTD DATTKAFLDE VDQLSQQNIS MDLPETLQSQ
360 370 380 390
AMLEKLMQTR VRNLLAQPAA GTTEAKPAPA PQADTPAAAP QGE
Length:393
Mass (Da):42,963
Last modified:July 1, 1989 - v1
Checksum:i9D272C6401D0E354
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13406 Genomic DNA. Translation: CAA31772.1.
X12614 Genomic DNA. Translation: CAA31134.1.
M87049 Genomic DNA. Translation: AAA67599.1.
U00096 Genomic DNA. Translation: AAC76806.1.
AP009048 Genomic DNA. Translation: BAE77498.1.
PIRiS02185.
RefSeqiNP_418247.1. NC_000913.3.
WP_000138997.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76806; AAC76806; b3803.
BAE77498; BAE77498; BAE77498.
GeneIDi948446.
KEGGiecj:JW3775.
eco:b3803.
PATRICifig|1411691.4.peg.2905.

Similar proteinsi

Entry informationi

Entry nameiHEMX_ECOLI
AccessioniPrimary (citable) accession number: P09127
Secondary accession number(s): Q2M8A8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: August 30, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways