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P09127 (HEMX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative uroporphyrinogen-III C-methyltransferase

Short name=Urogen-III methylase
EC=2.1.1.107
Alternative name(s):
ORF X
Gene names
Name:hemX
Ordered Locus Names:b3803, JW3775
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.

S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.

Subunit structure

Homooligomer, possibly a homopentamer. Ref.8

Subcellular location

Cell inner membrane; Single-pass membrane protein Ref.8.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

dapEP0AED71EBI-1128342,EBI-1129009

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393Putative uroporphyrinogen-III C-methyltransferase
PRO_0000135255

Regions

Transmembrane37 – 5721Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
P09127 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 9D272C6401D0E354

FASTA39342,963
        10         20         30         40         50         60 
MTEQEKTSAV VEETREAVDT TSQPVATEKK SKNNTALILS AVAIAIALAA GIGLYGWGKQ 

        70         80         90        100        110        120 
QAVNQTATSD ALANQLTALQ KAQESQKAEL EGIIKQQAAQ LKQANRQQET LAKQLDEVQQ 

       130        140        150        160        170        180 
KVATISGSDA KTWLLAQADF LVKLAGRKLW SDQDVTTAAA LLKSADASLA DMNDPSLITV 

       190        200        210        220        230        240 
RRAITDDIAS LSAVSQVDYD GIILKLNQLS NQVDNLRLAD NDSDGSPMDS DGEELSSSIS 

       250        260        270        280        290        300 
EWRINLQKSW QNFMDNFITI RRRDDTAVPL LAPNQDIYLR ENIRSRLLVA AQAVPRHQEE 

       310        320        330        340        350        360 
TYRQALENVS TWVRAYYDTD DATTKAFLDE VDQLSQQNIS MDLPETLQSQ AMLEKLMQTR 

       370        380        390 
VRNLLAQPAA GTTEAKPAPA PQADTPAAAP QGE 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the hemX gene, the third member of the Uro operon of Escherichia coli K12."
Sasarman A., Echelard Y., Letowski J., Tardif D., Drolet M.
Nucleic Acids Res. 16:11835-11835(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"The sequence of hemC, hemD and two additional E. coli genes."
Alefounder P.R., Abell C., Battersby A.R.
Nucleic Acids Res. 16:9871-9871(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / CS520.
[3]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-12.
Strain: K12 / EMG2.
[7]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[8]"Protein complexes of the Escherichia coli cell envelope."
Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.
J. Biol. Chem. 280:34409-34419(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION.
Strain: BL21-DE3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X13406 Genomic DNA. Translation: CAA31772.1.
X12614 Genomic DNA. Translation: CAA31134.1.
M87049 Genomic DNA. Translation: AAA67599.1.
U00096 Genomic DNA. Translation: AAC76806.1.
AP009048 Genomic DNA. Translation: BAE77498.1.
PIRS02185.
RefSeqNP_418247.1. NC_000913.3.
YP_491639.1. NC_007779.1.

3D structure databases

ProteinModelPortalP09127.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9888N.
IntActP09127. 3 interactions.
STRING511145.b3803.

Proteomic databases

PaxDbP09127.
PRIDEP09127.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76806; AAC76806; b3803.
BAE77498; BAE77498; BAE77498.
GeneID12930713.
948446.
KEGGecj:Y75_p3375.
eco:b3803.
PATRIC32123105. VBIEscCol129921_3918.

Organism-specific databases

EchoBASEEB0428.
EcoGeneEG10433. hemX.

Phylogenomic databases

eggNOGCOG2959.
HOGENOMHOG000270899.
KOK02496.
OMATRVRNLM.
OrthoDBEOG6QK4QM.
ProtClustDBPRK10920.

Enzyme and pathway databases

BioCycEcoCyc:HEMX-MONOMER.
ECOL316407:JW3775-MONOMER.
UniPathwayUPA00148; UER00211.

Gene expression databases

GenevestigatorP09127.

Family and domain databases

InterProIPR007470. HemX.
[Graphical view]
PfamPF04375. HemX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP09127.

Entry information

Entry nameHEMX_ECOLI
AccessionPrimary (citable) accession number: P09127
Secondary accession number(s): Q2M8A8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 19, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene