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P09126 (HEM4_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uroporphyrinogen-III synthase

Short name=UROS
EC=4.2.1.75
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene names
Name:hemD
Ordered Locus Names:b3804, JW3776
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III By similarity.

Catalytic activity

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.

Subunit structure

Monomer.

Sequence similarities

Belongs to the uroporphyrinogen-III synthase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from direct assay PubMed 8224882. Source: EcoCyc

   Molecular_functionuroporphyrinogen-III synthase activity

Inferred from direct assay PubMed 2557837. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246Uroporphyrinogen-III synthase
PRO_0000135243

Experimental info

Sequence conflict1461T → A in AAA23956. Ref.4
Sequence conflict2361A → R Ref.2
Sequence conflict2361A → R Ref.3

Sequences

Sequence LengthMass (Da)Tools
P09126 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 0BF7453C72395A0C

FASTA24627,798
        10         20         30         40         50         60 
MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL GESDLLFALS 

        70         80         90        100        110        120 
QHAVAFAQSQ LHQQDRKWPR LPDYFAIGRT TALALHTVSG QKILYPQDRE ISEVLLQLPE 

       130        140        150        160        170        180 
LQNIAGKRAL ILRGNGGREL IGDTLTARGA EVTFCECYQR CAIHYDGAEE AMRWQAREVT 

       190        200        210        220        230        240 
MVVVTSGEML QQLWSLIPQW YREHWLLHCR LLVVSERLAK LARELGWQDI KVADNADNDA 


LLRALQ 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of hemC, hemD and two additional E. coli genes."
Alefounder P.R., Abell C., Battersby A.R.
Nucleic Acids Res. 16:9871-9871(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / CS520.
[2]"Nucleotide sequence of hemD, the second gene in the hem operon of Escherichia coli K-12."
Jordan P.M., Mgbeje B.I.A., Alwan A.F., Thomas S.D.
Nucleic Acids Res. 15:10583-10583(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Nucleotide sequence for the hemD gene of Escherichia coli encoding uroporphyrinogen III synthase and initial evidence for a hem operon."
Jordan P.M., Mgbeje B.I.A., Thomas S.D., Alwan A.F.
Biochem. J. 249:613-616(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[4]"Molecular cloning and sequencing of the hemD gene of Escherichia coli K-12 and preliminary data on the Uro operon."
Sasarman A., Nepveu A., Echelard Y., Dymetryszyn J., Drolet M., Goyer C.
J. Bacteriol. 169:4257-4262(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[6]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[7]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X12614 Genomic DNA. Translation: CAA31133.1.
Y00883 Genomic DNA. Translation: CAA68776.1.
M17360 Genomic DNA. Translation: AAA23956.1.
M87049 Genomic DNA. Translation: AAA67600.1.
U00096 Genomic DNA. Translation: AAC76807.1.
AP009048 Genomic DNA. Translation: BAE77497.1.
PIRE65184.
RefSeqNP_418248.1. NC_000913.3.
YP_491638.1. NC_007779.1.

3D structure databases

ProteinModelPortalP09126.
SMRP09126. Positions 1-245.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP09126. 3 interactions.
STRING511145.b3804.

Proteomic databases

PRIDEP09126.

Protocols and materials databases

DNASU948587.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76807; AAC76807; b3804.
BAE77497; BAE77497; BAE77497.
GeneID12933615.
948587.
KEGGecj:Y75_p3374.
eco:b3804.
PATRIC32123107. VBIEscCol129921_3919.

Organism-specific databases

EchoBASEEB0425.
EcoGeneEG10430. hemD.

Phylogenomic databases

eggNOGCOG1587.
HOGENOMHOG000247993.
KOK01719.
OMAVRYWEVY.
OrthoDBEOG6RZB2F.
ProtClustDBPRK05928.

Enzyme and pathway databases

BioCycEcoCyc:UROGENIIISYN-MONOMER.
ECOL316407:JW3776-MONOMER.
MetaCyc:UROGENIIISYN-MONOMER.
UniPathwayUPA00251; UER00320.

Gene expression databases

GenevestigatorP09126.

Family and domain databases

InterProIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMSSF69618. SSF69618. 1 hit.
ProtoNetSearch...

Other

PROP09126.

Entry information

Entry nameHEM4_ECOLI
AccessionPrimary (citable) accession number: P09126
Secondary accession number(s): Q2M8A9, Q47250
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 19, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene