P09126 (HEM4_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uroporphyrinogen-III synthase
Short name=UROS
EC=4.2.1.75

Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase

Gene names

Name:	hemD
Ordered Locus Names:	b3804, JW3776

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III By similarity.
Catalytic activity	Hydroxymethylbilane = uroporphyrinogen III + H2O.
Pathway	Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.
Subunit structure	Monomer.
Sequence similarities	Belongs to the uroporphyrinogen-III synthase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	uroporphyrinogen-III synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Uroporphyrinogen-III synthase		PRO_0000135243
Experimental info
	Sequence conflict	146	1	T → A in AAA23956. Ref.4
	Sequence conflict	236	1	A → R Ref.2
	Sequence conflict	236	1	A → R Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P09126 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 0BF7453C72395A0C	FASTA	246	27,798
	10 20 30 40 50 60 MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL GESDLLFALS 70 80 90 100 110 120 QHAVAFAQSQ LHQQDRKWPR LPDYFAIGRT TALALHTVSG QKILYPQDRE ISEVLLQLPE 130 140 150 160 170 180 LQNIAGKRAL ILRGNGGREL IGDTLTARGA EVTFCECYQR CAIHYDGAEE AMRWQAREVT 190 200 210 220 230 240 MVVVTSGEML QQLWSLIPQW YREHWLLHCR LLVVSERLAK LARELGWQDI KVADNADNDA LLRALQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of hemC, hemD and two additional E. coli genes." Alefounder P.R., Abell C., Battersby A.R. Nucleic Acids Res. 16:9871-9871(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / CS520.
[2]	"Nucleotide sequence of hemD, the second gene in the hem operon of Escherichia coli K-12." Jordan P.M., Mgbeje B.I.A., Alwan A.F., Thomas S.D. Nucleic Acids Res. 15:10583-10583(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Nucleotide sequence for the hemD gene of Escherichia coli encoding uroporphyrinogen III synthase and initial evidence for a hem operon." Jordan P.M., Mgbeje B.I.A., Thomas S.D., Alwan A.F. Biochem. J. 249:613-616(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"Molecular cloning and sequencing of the hemD gene of Escherichia coli K-12 and preliminary data on the Uro operon." Sasarman A., Nepveu A., Echelard Y., Dymetryszyn J., Drolet M., Goyer C. J. Bacteriol. 169:4257-4262(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X12614 Genomic DNA. Translation: CAA31133.1. Y00883 Genomic DNA. Translation: CAA68776.1. M17360 Genomic DNA. Translation: AAA23956.1. M87049 Genomic DNA. Translation: AAA67600.1. U00096 Genomic DNA. Translation: AAC76807.1. AP009048 Genomic DNA. Translation: BAE77497.1.
PIR	E65184.
RefSeq	NP_418248.1. NC_000913.2. YP_491638.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P09126.
SMR	P09126. Positions 1-245.
ModBase	Search...
Protein-protein interaction databases
IntAct	P09126. 3 interactions.
STRING	511145.b3804.
Proteomic databases
PRIDE	P09126.
Protocols and materials databases
DNASU	948587.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76807; AAC76807; b3804. BAE77497; BAE77497; BAE77497.
GeneID	12933615. 948587.
KEGG	ecj:Y75_p3374. eco:b3804.
PATRIC	32123107. VBIEscCol129921_3919.
Organism-specific databases
EchoBASE	EB0425.
EcoGene	EG10430. hemD.
Phylogenomic databases
eggNOG	COG1587.
HOGENOM	HOG000247993.
KO	K01719.
OMA	VRYWEVY.
ProtClustDB	PRK05928.
Enzyme and pathway databases
BioCyc	EcoCyc:UROGENIIISYN-MONOMER. ECOL316407:JW3776-MONOMER. MetaCyc:UROGENIIISYN-MONOMER.
UniPathway	UPA00251; UER00320.
Gene expression databases
Genevestigator	P09126.
Family and domain databases
InterPro	IPR003754. 4pyrrol_synth_uPrphyn_synth. [Graphical view]
Pfam	PF02602. HEM4. 1 hit. [Graphical view]
SUPFAM	SSF69618. HEM4_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEM4_ECOLI

Accession

Primary (citable) accession number: P09126
Secondary accession number(s): Q2M8A9, Q47250

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 29, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program