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Protein

Uroporphyrinogen-III synthase

Gene

hemD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. Uroporphyrinogen-III synthase (hemD)
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • uroporphyrinogen-III synthase activity Source: EcoCyc

GO - Biological processi

  • protoporphyrinogen IX biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:UROGENIIISYN-MONOMER.
ECOL316407:JW3776-MONOMER.
MetaCyc:UROGENIIISYN-MONOMER.
UniPathwayiUPA00251; UER00320.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase (EC:4.2.1.75)
Short name:
UROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:hemD
Ordered Locus Names:b3804, JW3776
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10430. hemD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352431 – 246Uroporphyrinogen-III synthaseAdd BLAST246

Proteomic databases

PaxDbiP09126.
PRIDEiP09126.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP09126. 3 interactors.
STRINGi511145.b3804.

Structurei

3D structure databases

ProteinModelPortaliP09126.
SMRiP09126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG41076E6. Bacteria.
COG1587. LUCA.
HOGENOMiHOG000247993.
InParanoidiP09126.
KOiK01719.
OMAiFISTNAV.
PhylomeDBiP09126.

Family and domain databases

CDDicd06578. HemD. 1 hit.
InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.

Sequencei

Sequence statusi: Complete.

P09126-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL
60 70 80 90 100
GESDLLFALS QHAVAFAQSQ LHQQDRKWPR LPDYFAIGRT TALALHTVSG
110 120 130 140 150
QKILYPQDRE ISEVLLQLPE LQNIAGKRAL ILRGNGGREL IGDTLTARGA
160 170 180 190 200
EVTFCECYQR CAIHYDGAEE AMRWQAREVT MVVVTSGEML QQLWSLIPQW
210 220 230 240
YREHWLLHCR LLVVSERLAK LARELGWQDI KVADNADNDA LLRALQ
Length:246
Mass (Da):27,798
Last modified:July 1, 1989 - v1
Checksum:i0BF7453C72395A0C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146T → A in AAA23956 (PubMed:3040684).Curated1
Sequence conflicti236A → R (PubMed:3320969).Curated1
Sequence conflicti236A → R (PubMed:3277628).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12614 Genomic DNA. Translation: CAA31133.1.
Y00883 Genomic DNA. Translation: CAA68776.1.
M17360 Genomic DNA. Translation: AAA23956.1.
M87049 Genomic DNA. Translation: AAA67600.1.
U00096 Genomic DNA. Translation: AAC76807.1.
AP009048 Genomic DNA. Translation: BAE77497.1.
PIRiE65184.
RefSeqiNP_418248.1. NC_000913.3.
WP_000026046.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76807; AAC76807; b3804.
BAE77497; BAE77497; BAE77497.
GeneIDi948587.
KEGGiecj:JW3776.
eco:b3804.
PATRICi32123107. VBIEscCol129921_3919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12614 Genomic DNA. Translation: CAA31133.1.
Y00883 Genomic DNA. Translation: CAA68776.1.
M17360 Genomic DNA. Translation: AAA23956.1.
M87049 Genomic DNA. Translation: AAA67600.1.
U00096 Genomic DNA. Translation: AAC76807.1.
AP009048 Genomic DNA. Translation: BAE77497.1.
PIRiE65184.
RefSeqiNP_418248.1. NC_000913.3.
WP_000026046.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP09126.
SMRiP09126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09126. 3 interactors.
STRINGi511145.b3804.

Proteomic databases

PaxDbiP09126.
PRIDEiP09126.

Protocols and materials databases

DNASUi948587.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76807; AAC76807; b3804.
BAE77497; BAE77497; BAE77497.
GeneIDi948587.
KEGGiecj:JW3776.
eco:b3804.
PATRICi32123107. VBIEscCol129921_3919.

Organism-specific databases

EchoBASEiEB0425.
EcoGeneiEG10430. hemD.

Phylogenomic databases

eggNOGiENOG41076E6. Bacteria.
COG1587. LUCA.
HOGENOMiHOG000247993.
InParanoidiP09126.
KOiK01719.
OMAiFISTNAV.
PhylomeDBiP09126.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00320.
BioCyciEcoCyc:UROGENIIISYN-MONOMER.
ECOL316407:JW3776-MONOMER.
MetaCyc:UROGENIIISYN-MONOMER.

Miscellaneous databases

PROiP09126.

Family and domain databases

CDDicd06578. HemD. 1 hit.
InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHEM4_ECOLI
AccessioniPrimary (citable) accession number: P09126
Secondary accession number(s): Q2M8A9, Q47250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.