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Protein

Uroporphyrinogen-III synthase

Gene

hemD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi

GO - Molecular functioni

  1. uroporphyrinogen-III synthase activity Source: EcoCyc

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:UROGENIIISYN-MONOMER.
ECOL316407:JW3776-MONOMER.
MetaCyc:UROGENIIISYN-MONOMER.
UniPathwayiUPA00251; UER00320.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase (EC:4.2.1.75)
Short name:
UROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:hemD
Ordered Locus Names:b3804, JW3776
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10430. hemD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246Uroporphyrinogen-III synthasePRO_0000135243Add
BLAST

Proteomic databases

PRIDEiP09126.

Expressioni

Gene expression databases

GenevestigatoriP09126.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP09126. 3 interactions.
STRINGi511145.b3804.

Structurei

3D structure databases

ProteinModelPortaliP09126.
SMRiP09126. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1587.
HOGENOMiHOG000247993.
InParanoidiP09126.
KOiK01719.
OMAiVRYWEVY.
OrthoDBiEOG6RZB2F.
PhylomeDBiP09126.

Family and domain databases

InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.

Sequencei

Sequence statusi: Complete.

P09126-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL
60 70 80 90 100
GESDLLFALS QHAVAFAQSQ LHQQDRKWPR LPDYFAIGRT TALALHTVSG
110 120 130 140 150
QKILYPQDRE ISEVLLQLPE LQNIAGKRAL ILRGNGGREL IGDTLTARGA
160 170 180 190 200
EVTFCECYQR CAIHYDGAEE AMRWQAREVT MVVVTSGEML QQLWSLIPQW
210 220 230 240
YREHWLLHCR LLVVSERLAK LARELGWQDI KVADNADNDA LLRALQ
Length:246
Mass (Da):27,798
Last modified:July 1, 1989 - v1
Checksum:i0BF7453C72395A0C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti146 – 1461T → A in AAA23956. (PubMed:3040684)Curated
Sequence conflicti236 – 2361A → R(PubMed:3320969)Curated
Sequence conflicti236 – 2361A → R(PubMed:3277628)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12614 Genomic DNA. Translation: CAA31133.1.
Y00883 Genomic DNA. Translation: CAA68776.1.
M17360 Genomic DNA. Translation: AAA23956.1.
M87049 Genomic DNA. Translation: AAA67600.1.
U00096 Genomic DNA. Translation: AAC76807.1.
AP009048 Genomic DNA. Translation: BAE77497.1.
PIRiE65184.
RefSeqiNP_418248.1. NC_000913.3.
YP_491638.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76807; AAC76807; b3804.
BAE77497; BAE77497; BAE77497.
GeneIDi12933615.
948587.
KEGGiecj:Y75_p3374.
eco:b3804.
PATRICi32123107. VBIEscCol129921_3919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12614 Genomic DNA. Translation: CAA31133.1.
Y00883 Genomic DNA. Translation: CAA68776.1.
M17360 Genomic DNA. Translation: AAA23956.1.
M87049 Genomic DNA. Translation: AAA67600.1.
U00096 Genomic DNA. Translation: AAC76807.1.
AP009048 Genomic DNA. Translation: BAE77497.1.
PIRiE65184.
RefSeqiNP_418248.1. NC_000913.3.
YP_491638.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP09126.
SMRiP09126. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09126. 3 interactions.
STRINGi511145.b3804.

Proteomic databases

PRIDEiP09126.

Protocols and materials databases

DNASUi948587.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76807; AAC76807; b3804.
BAE77497; BAE77497; BAE77497.
GeneIDi12933615.
948587.
KEGGiecj:Y75_p3374.
eco:b3804.
PATRICi32123107. VBIEscCol129921_3919.

Organism-specific databases

EchoBASEiEB0425.
EcoGeneiEG10430. hemD.

Phylogenomic databases

eggNOGiCOG1587.
HOGENOMiHOG000247993.
InParanoidiP09126.
KOiK01719.
OMAiVRYWEVY.
OrthoDBiEOG6RZB2F.
PhylomeDBiP09126.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00320.
BioCyciEcoCyc:UROGENIIISYN-MONOMER.
ECOL316407:JW3776-MONOMER.
MetaCyc:UROGENIIISYN-MONOMER.

Miscellaneous databases

PROiP09126.

Gene expression databases

GenevestigatoriP09126.

Family and domain databases

InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of hemC, hemD and two additional E. coli genes."
    Alefounder P.R., Abell C., Battersby A.R.
    Nucleic Acids Res. 16:9871-9871(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / CS520.
  2. "Nucleotide sequence of hemD, the second gene in the hem operon of Escherichia coli K-12."
    Jordan P.M., Mgbeje B.I.A., Alwan A.F., Thomas S.D.
    Nucleic Acids Res. 15:10583-10583(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Nucleotide sequence for the hemD gene of Escherichia coli encoding uroporphyrinogen III synthase and initial evidence for a hem operon."
    Jordan P.M., Mgbeje B.I.A., Thomas S.D., Alwan A.F.
    Biochem. J. 249:613-616(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  4. "Molecular cloning and sequencing of the hemD gene of Escherichia coli K-12 and preliminary data on the Uro operon."
    Sasarman A., Nepveu A., Echelard Y., Dymetryszyn J., Drolet M., Goyer C.
    J. Bacteriol. 169:4257-4262(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiHEM4_ECOLI
AccessioniPrimary (citable) accession number: P09126
Secondary accession number(s): Q2M8A9, Q47250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 7, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.