Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P09126

- HEM4_ECOLI

UniProt

P09126 - HEM4_ECOLI

Protein

Uroporphyrinogen-III synthase

Gene

hemD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.By similarity

    Catalytic activityi

    Hydroxymethylbilane = uroporphyrinogen III + H2O.

    Pathwayi

    GO - Molecular functioni

    1. uroporphyrinogen-III synthase activity Source: EcoCyc

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Porphyrin biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:UROGENIIISYN-MONOMER.
    ECOL316407:JW3776-MONOMER.
    MetaCyc:UROGENIIISYN-MONOMER.
    UniPathwayiUPA00251; UER00320.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uroporphyrinogen-III synthase (EC:4.2.1.75)
    Short name:
    UROS
    Alternative name(s):
    Hydroxymethylbilane hydrolyase [cyclizing]
    Uroporphyrinogen-III cosynthase
    Gene namesi
    Name:hemD
    Ordered Locus Names:b3804, JW3776
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10430. hemD.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246Uroporphyrinogen-III synthasePRO_0000135243Add
    BLAST

    Proteomic databases

    PRIDEiP09126.

    Expressioni

    Gene expression databases

    GenevestigatoriP09126.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    IntActiP09126. 3 interactions.
    STRINGi511145.b3804.

    Structurei

    3D structure databases

    ProteinModelPortaliP09126.
    SMRiP09126. Positions 1-245.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1587.
    HOGENOMiHOG000247993.
    KOiK01719.
    OMAiVRYWEVY.
    OrthoDBiEOG6RZB2F.
    PhylomeDBiP09126.

    Family and domain databases

    InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
    [Graphical view]
    PfamiPF02602. HEM4. 1 hit.
    [Graphical view]
    SUPFAMiSSF69618. SSF69618. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P09126-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL    50
    GESDLLFALS QHAVAFAQSQ LHQQDRKWPR LPDYFAIGRT TALALHTVSG 100
    QKILYPQDRE ISEVLLQLPE LQNIAGKRAL ILRGNGGREL IGDTLTARGA 150
    EVTFCECYQR CAIHYDGAEE AMRWQAREVT MVVVTSGEML QQLWSLIPQW 200
    YREHWLLHCR LLVVSERLAK LARELGWQDI KVADNADNDA LLRALQ 246
    Length:246
    Mass (Da):27,798
    Last modified:July 1, 1989 - v1
    Checksum:i0BF7453C72395A0C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti146 – 1461T → A in AAA23956. (PubMed:3040684)Curated
    Sequence conflicti236 – 2361A → R(PubMed:3320969)Curated
    Sequence conflicti236 – 2361A → R(PubMed:3277628)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12614 Genomic DNA. Translation: CAA31133.1.
    Y00883 Genomic DNA. Translation: CAA68776.1.
    M17360 Genomic DNA. Translation: AAA23956.1.
    M87049 Genomic DNA. Translation: AAA67600.1.
    U00096 Genomic DNA. Translation: AAC76807.1.
    AP009048 Genomic DNA. Translation: BAE77497.1.
    PIRiE65184.
    RefSeqiNP_418248.1. NC_000913.3.
    YP_491638.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76807; AAC76807; b3804.
    BAE77497; BAE77497; BAE77497.
    GeneIDi12933615.
    948587.
    KEGGiecj:Y75_p3374.
    eco:b3804.
    PATRICi32123107. VBIEscCol129921_3919.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12614 Genomic DNA. Translation: CAA31133.1 .
    Y00883 Genomic DNA. Translation: CAA68776.1 .
    M17360 Genomic DNA. Translation: AAA23956.1 .
    M87049 Genomic DNA. Translation: AAA67600.1 .
    U00096 Genomic DNA. Translation: AAC76807.1 .
    AP009048 Genomic DNA. Translation: BAE77497.1 .
    PIRi E65184.
    RefSeqi NP_418248.1. NC_000913.3.
    YP_491638.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P09126.
    SMRi P09126. Positions 1-245.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P09126. 3 interactions.
    STRINGi 511145.b3804.

    Proteomic databases

    PRIDEi P09126.

    Protocols and materials databases

    DNASUi 948587.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76807 ; AAC76807 ; b3804 .
    BAE77497 ; BAE77497 ; BAE77497 .
    GeneIDi 12933615.
    948587.
    KEGGi ecj:Y75_p3374.
    eco:b3804.
    PATRICi 32123107. VBIEscCol129921_3919.

    Organism-specific databases

    EchoBASEi EB0425.
    EcoGenei EG10430. hemD.

    Phylogenomic databases

    eggNOGi COG1587.
    HOGENOMi HOG000247993.
    KOi K01719.
    OMAi VRYWEVY.
    OrthoDBi EOG6RZB2F.
    PhylomeDBi P09126.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00320 .
    BioCyci EcoCyc:UROGENIIISYN-MONOMER.
    ECOL316407:JW3776-MONOMER.
    MetaCyc:UROGENIIISYN-MONOMER.

    Miscellaneous databases

    PROi P09126.

    Gene expression databases

    Genevestigatori P09126.

    Family and domain databases

    InterProi IPR003754. 4pyrrol_synth_uPrphyn_synth.
    [Graphical view ]
    Pfami PF02602. HEM4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69618. SSF69618. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of hemC, hemD and two additional E. coli genes."
      Alefounder P.R., Abell C., Battersby A.R.
      Nucleic Acids Res. 16:9871-9871(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / CS520.
    2. "Nucleotide sequence of hemD, the second gene in the hem operon of Escherichia coli K-12."
      Jordan P.M., Mgbeje B.I.A., Alwan A.F., Thomas S.D.
      Nucleic Acids Res. 15:10583-10583(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. "Nucleotide sequence for the hemD gene of Escherichia coli encoding uroporphyrinogen III synthase and initial evidence for a hem operon."
      Jordan P.M., Mgbeje B.I.A., Thomas S.D., Alwan A.F.
      Biochem. J. 249:613-616(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    4. "Molecular cloning and sequencing of the hemD gene of Escherichia coli K-12 and preliminary data on the Uro operon."
      Sasarman A., Nepveu A., Echelard Y., Dymetryszyn J., Drolet M., Goyer C.
      J. Bacteriol. 169:4257-4262(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
      Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
      Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    7. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiHEM4_ECOLI
    AccessioniPrimary (citable) accession number: P09126
    Secondary accession number(s): Q2M8A9, Q47250
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3