Reviewed, UniProtKB/Swiss-Prot P09122 (DPO3X_BACSU)

Names and origin

Protein names

Recommended name:
    DNA polymerase III subunit gamma/tau
    EC=2.7.7.7

Gene names

Name:	dnaX
Synonyms:	dnaH
Ordered Locus Names:	BSU00190

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	DNA polymerase III subunit gamma/tau		PRO_0000105492
Regions
	Nucleotide binding	45 – 52	8	ATP Potential
Experimental info
	Sequence conflict	533	1	E → A in CAA34877. Ref.1
	Sequence conflict	533	1	E → A in BAA05255. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P09122-1 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: F5975C246622E4F5	FASTA	563	62,763
	10 20 30 40 50 60 MSYQALYRVF RPQRFEDVVG QEHITKTLQN ALLQKKFSHA YLFSGPRGTG KTSAAKIFAK 70 80 90 100 110 120 AVNCEHAPVD EPCNECAACK GITNGSISDV IEIDAASNNG VDEIRDIRDK VKFAPSAVTY 130 140 150 160 170 180 KVYIIDEVHM LSIGAFNALL KTLEEPPEHC IFILATTEPH KIPLTIISRC QRFDFKRITS 190 200 210 220 230 240 QAIVGRMNKI VDAEQLQVEE GSLEIIASAA DGGMRDALSL LDQAISFSGD ILKVEDALLI 250 260 270 280 290 300 TGAVSQLYIG KLAKSLHDKN VSDALETLNE LLQQGKDPAK LIEDMIFYFR DMLLYKTAPG 310 320 330 340 350 360 LEGVLEKVKV DETFRELSEQ IPAQALYEMI DILNKSHQEM KWTNHPRIFF EVAVVKICQT 370 380 390 400 410 420 SHQSAADLPE VDMLMKKIQQ LEQEVERLKT TGIKAAAESP KKEAPRVPKG GKSNYKAPVG 430 440 450 460 470 480 RIHEILKEAT RPDLDLLRNS WGKLLAHLKQ QNKVSHAALL NDSEPVAAGS AAFVLKFKYE 490 500 510 520 530 540 IHCKMVAEDN NGVRTNLEQI LESMLGKRMD LIGVPEAQWG KIREEFLEDH QQENEGSNEP 550 560 AEEDPLIAEA KKLVGADLIE IKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, genetic characterization and DNA sequence analysis of the recM region of Bacillus subtilis." Alonso J.C., Shirahige K., Ogasawara N. Nucleic Acids Res. 18:6771-6777(1990) [PubMed: 2124672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / YB886 / BG214.
[2]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 211 AND 533.
[5]	"A dnaZX-like open reading frame downstream from the Bacillus subtilis scRNA gene." Struck J.C.R., Vogel D.W., Ulbrich N., Erdmann V.A. Nucleic Acids Res. 16:2720-2720(1988) [PubMed: 2452406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-422. Strain: 168.
[6]	"Transcription and processing of Bacillus subtilis small cytoplasmic RNA." Struck J.C.R., Hartmann R.K., Toschka H.Y., Erdmann V.A. Mol. Gen. Genet. 215:478-482(1989) [PubMed: 2468993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5. Strain: 168.
[7]	"Characterization of a 17 kDa protein gene upstream from the small cytoplasmic RNA gene of Bacillus subtilis." Struck J.C.R., Kretschmer-Kazemi F.R., Schroeder W., Hucho F., Toschka H.Y., Erdmann V.A. Biochim. Biophys. Acta 1050:80-83(1990) [PubMed: 1698458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17014 Genomic DNA. Translation: CAA34877.1. D26185 Genomic DNA. Translation: BAA05255.1. AL009126 Genomic DNA. Translation: CAB11795.2. X06803 Genomic DNA. Translation: CAA29958.1. X52144 Genomic DNA. Translation: CAA36390.1.
PIR	I40469. S13786.
RefSeq	NP_387900.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936899.
GenomeReviews	Gene locus BSU00190 in contig AL009126_GR.
KEGG	bsu:BSU00190.
Organism-specific databases
SubtiList	BG10083. dnaX. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729459.
Enzyme and pathway databases
BRENDA	2.7.7.7. 150.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR001270. Chaprnin_clpA/B. IPR008921. DNA_pol3_clamp-load_cplx_C. IPR012763. DNA_pol_III_sug/sutau. [Graphical view]
Pfam	PF00004. AAA. 1 hit. [Graphical view]
PRINTS	PR00300. CLPPROTEASEA.
SMART	SM00382. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPO3X_BACSU

Accession

Primary (citable) accession number: P09122

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	June 16, 2009
Last modified:	December 15, 2009
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)