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P09122 (DPO3X_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit gamma/tau
EC=2.7.7.7
Gene names
Name:dnaX
Synonyms:dnaH
Ordered Locus Names:BSU00190
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length563 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 563563DNA polymerase III subunit gamma/tau
PRO_0000105492

Regions

Nucleotide binding45 – 528ATP Potential

Experimental info

Sequence conflict5331E → A in CAA34877. Ref.1
Sequence conflict5331E → A in BAA05255. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P09122 [UniParc].

Last modified June 16, 2009. Version 3.
Checksum: F5975C246622E4F5

FASTA56362,763
        10         20         30         40         50         60 
MSYQALYRVF RPQRFEDVVG QEHITKTLQN ALLQKKFSHA YLFSGPRGTG KTSAAKIFAK 

        70         80         90        100        110        120 
AVNCEHAPVD EPCNECAACK GITNGSISDV IEIDAASNNG VDEIRDIRDK VKFAPSAVTY 

       130        140        150        160        170        180 
KVYIIDEVHM LSIGAFNALL KTLEEPPEHC IFILATTEPH KIPLTIISRC QRFDFKRITS 

       190        200        210        220        230        240 
QAIVGRMNKI VDAEQLQVEE GSLEIIASAA DGGMRDALSL LDQAISFSGD ILKVEDALLI 

       250        260        270        280        290        300 
TGAVSQLYIG KLAKSLHDKN VSDALETLNE LLQQGKDPAK LIEDMIFYFR DMLLYKTAPG 

       310        320        330        340        350        360 
LEGVLEKVKV DETFRELSEQ IPAQALYEMI DILNKSHQEM KWTNHPRIFF EVAVVKICQT 

       370        380        390        400        410        420 
SHQSAADLPE VDMLMKKIQQ LEQEVERLKT TGIKAAAESP KKEAPRVPKG GKSNYKAPVG 

       430        440        450        460        470        480 
RIHEILKEAT RPDLDLLRNS WGKLLAHLKQ QNKVSHAALL NDSEPVAAGS AAFVLKFKYE 

       490        500        510        520        530        540 
IHCKMVAEDN NGVRTNLEQI LESMLGKRMD LIGVPEAQWG KIREEFLEDH QQENEGSNEP 

       550        560 
AEEDPLIAEA KKLVGADLIE IKD

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning, genetic characterization and DNA sequence analysis of the recM region of Bacillus subtilis."
Alonso J.C., Shirahige K., Ogasawara N.
Nucleic Acids Res. 18:6771-6777(1990) [PubMed: 2124672] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / YB886 / BG214.
[2]"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
Ogasawara N., Nakai S., Yoshikawa H.
DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 211 AND 533.
[5]"A dnaZX-like open reading frame downstream from the Bacillus subtilis scRNA gene."
Struck J.C.R., Vogel D.W., Ulbrich N., Erdmann V.A.
Nucleic Acids Res. 16:2720-2720(1988) [PubMed: 2452406] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-422.
Strain: 168.
[6]"Transcription and processing of Bacillus subtilis small cytoplasmic RNA."
Struck J.C.R., Hartmann R.K., Toschka H.Y., Erdmann V.A.
Mol. Gen. Genet. 215:478-482(1989) [PubMed: 2468993] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
Strain: 168.
[7]"Characterization of a 17 kDa protein gene upstream from the small cytoplasmic RNA gene of Bacillus subtilis."
Struck J.C.R., Kretschmer-Kazemi F.R., Schroeder W., Hucho F., Toschka H.Y., Erdmann V.A.
Biochim. Biophys. Acta 1050:80-83(1990) [PubMed: 1698458] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
Strain: 168.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X17014 Genomic DNA. Translation: CAA34877.1.
D26185 Genomic DNA. Translation: BAA05255.1.
AL009126 Genomic DNA. Translation: CAB11795.2.
X06803 Genomic DNA. Translation: CAA29958.1.
X52144 Genomic DNA. Translation: CAA36390.1.
PIRI40469.
S13786.
RefSeqNP_387900.2. NC_000964.3.

3D structure databases

ProteinModelPortalP09122.
SMRP09122. Positions 1-367.
ModBaseSearch...

Protein-protein interaction databases

IntActP09122. 7 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000003868; EBBACP00000003868; EBBACG00000003860.
GeneID936899.
GenomeReviewsGene locus BSU00190 in contig AL009126_GR.
KEGGbsu:BSU00190.
PATRIC18971499. VBIBacSub10457_0020.

Organism-specific databases

GenoListBSU00190. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000000361.
HOGENOMHBG729459.
PhylomeDBP09122.
ProtClustDBPRK05563.

Enzyme and pathway databases

BioCycBSUB:BSU00190-MONOMER.

Family and domain databases

InterProIPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR001270. Chaprnin_ClpA/B.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR022754. DNA_pol_III_gamma-3.
IPR012763. DNA_pol_III_sug/sutau.
[Graphical view]
KOK02343.
PfamPF00004. AAA. 1 hit.
PF12169. DNA_pol3_gamma3. 1 hit.
[Graphical view]
PRINTSPR00300. CLPPROTEASEA.
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF48019. Pol_clamp_load_C. 1 hit.
TIGRFAMsTIGR02397. DnaX_nterm. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDPO3X_BACSU
AccessionPrimary (citable) accession number: P09122
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 16, 2009
Last modified: January 25, 2012
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents