Reviewed, UniProtKB/Swiss-Prot P09121 (CDGT_BACS3)

Names and origin

Protein names

Recommended name:
    Cyclomaltodextrin glucanotransferase
    EC=2.4.1.19
Alternative name(s):
    Cyclodextrin-glycosyltransferase
      Short name=CGTase

Gene names

Name:

cgt

Organism

Bacillus sp. (strain 38-2)

Taxonomic identifier

1412 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	712 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Secreted By similarity.
Domain	May consist of two protein domains: the one in the N-terminal side cleaves the alpha-1,4-glucosidic bond in starch, and the other in the C-terminal side catalyzes other activities, including the reconstitution of an alpha-1,4-glucosidic linkage for cyclizing the maltooligosaccharide produced.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. Contains 1 CBM20 (carbohydrate binding type-20) domain. Contains 1 IPT/TIG domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate binding Inferred from electronic annotation. Source: InterPro cyclomaltodextrin glucanotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.1
	Chain	28 – 712	685	Cyclomaltodextrin glucanotransferase		PRO_0000001439
Regions
	Domain	526 – 606	81	IPT/TIG
	Domain	607 – 712	106	CBM20
	Region	28 – 165	138	A1
	Region	166 – 229	64	B
	Region	230 – 433	204	A2
	Region	434 – 522	89	C
	Region	523 – 608	86	D
	Region	609 – 712	104	E
Sites
	Active site	256	1	Nucleophile By similarity
	Active site	284	1	Proton donor By similarity
	Active site	355	1	By similarity
	Metal binding	54	1	Calcium 2 By similarity
	Metal binding	56	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	59	1	Calcium 2 By similarity
	Metal binding	60	1	Calcium 2 By similarity
	Metal binding	78	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium 2 By similarity
	Metal binding	166	1	Calcium 1 By similarity
	Metal binding	217	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	226	1	Calcium 1 By similarity
	Metal binding	260	1	Calcium 1; via carbonyl oxygen By similarity
Amino acid modifications
	Disulfide bond	70 ↔ 77		By similarity
Experimental info
	Sequence conflict	582 – 586	5	VPGGI → SWRHL Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P09121-1 [UniParc]. Last modified July 1, 1993. Version 2. Checksum: 4FAA8F70BEF818F9	FASTA	712	78,249
	10 20 30 40 50 60 MKRFMKLTAV WTLWLSLTLG LLSPVHAAPD TSVSNKQNFS TDVIYQIFTD RFSDGNPANN 70 80 90 100 110 120 PTGAAFDGSC TNLRLYCGGD WQGIINKIND GYLTGMGITA IWISQPVENI YSVINYSGVH 130 140 150 160 170 180 NTAYHGYWAR DFKKTNPAYG TMQDFKNLID TAHAHNIKVI IDFAPNHTSP ASSDDPSFAE 190 200 210 220 230 240 NGRLYDNGNL LGGYTNDTQN LFHHYGGTDF STIENGIYKN LYDLADLNHN NSSVDVYLKD 250 260 270 280 290 300 AIKMWLDLGV DGIRVDAVKH MPFGWQKSFM STINNYKPVF NFGEWFLGVN EISPEYHQFA 310 320 330 340 350 360 NESGMSLLDF PFAQKARQVF RDNTDNMYGL KAMLEGSEVD YAQVNDQVTF IDNHDMERFH 370 380 390 400 410 420 TSNGDRRKLE QALAFTLTSR GVPAIYYGSE QYMSGGNDPD NRARIPSFST TTTAYQVIQK 430 440 450 460 470 480 LAPLRKSNPA IAYGSTQERW INNDVIIYER KFGNNVAVVA INRNMNTPAS ITGLVTSLPQ 490 500 510 520 530 540 GSYNDVLGGI LNGNTLTVGA GGAASNFTLA PGGTAVWQYT TDATAPINGN VGPMMAKAGV 550 560 570 580 590 600 TITIDGRASA RQGTVYFGTT AVTGADIVAW EDTQIQVKIL RVPGGIYDIR VANAAGAASN 610 620 630 640 650 660 IYDNFEVLTG DQVTVRFVIN NATTALGQNV FLTGNVSELG NWDPNNAIGP MYNQVVYQYP 670 680 690 700 710 TWYYDVSVPA GQTIEFKFLK KQGSTVTWEG GANRTFTTPT SGTATVNVNW QP

References

[1]	"Molecular cloning and nucleotide sequence of the cyclomaltodextrin glucanotransferase gene from the alkalophilic Bacillus sp. strain no. 38-2." Kaneko T., Hamamoto T., Horikoshi K. J. Gen. Microbiol. 134:97-105(1988) [PubMed: 2972812] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-44.
[2]	"Nucleotide sequence of the cyclomaltodextrin glucanotransferase (CGTase) gene from alkalophilic Bacillus sp. strain No. 38-2." Hamamoto T., Kaneko T., Horikoshi K. Agric. Biol. Chem. 51:2019-2022(1987) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-586.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M19880 Genomic DNA. Translation: AAA22309.1. D00129 Genomic DNA. Translation: BAA00077.1.
3D structure databases
HSSP	HSSP built from PDB template 1PAM based on UniProtKB P05618.
SMR	P09121. Positions 28-712.
ModBase	Search...
Protein family/group databases
CAZy	CBM20. Carbohydrate-Binding Module Family 20. GH13. Glycoside Hydrolase Family 13.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR002044. Glyco_hydro_carb-bd. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR002909. IPT_TIG_rcpt. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF00686. CBM_20. 1 hit. PF01833. TIG. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
ProDom	PD001568. Glyco_hydro_CBD. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00642. Aamy. 1 hit. SM00632. Aamy_C. 1 hit. [Graphical view]
PROSITE	PS51166. CBM20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDGT_BACS3

Accession

Primary (citable) accession number: P09121

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)