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UniProtKB - P09119 (CDC6_YEAST)

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Protein

Cell division control protein 6

Gene

CDC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a crucial role in forming the pre-replicative complexes. Interacts with the origin recognition complex (ORC) and MCM2-7 helicase complex leading to the linking of those complexes and loading of the replicative helicase MCM2-7 onto the pre-replicative complexes. Required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.9 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 115ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: SGD
  • chromatin binding Source: SGD
  • DNA replication origin binding Source: SGD
  • GTPase activity Source: SGD
  • GTP binding Source: SGD
Complete GO annotation...

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • G1/S transition of mitotic cell cycle Source: SGD
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
  • regulation of chromatin silencing at telomere Source: SGD
  • regulation of DNA-dependent DNA replication initiation Source: SGD
Complete GO annotation...

Keywordsi

Biological processCell cycle, Cell division, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31626-MONOMER.
ReactomeiR-SCE-68616. Assembly of the ORC complex at the origin of replication.
R-SCE-68689. CDC6 association with the ORC:origin complex.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-68962. Activation of the pre-replicative complex.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69298. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 6
Gene namesi
Name:CDC6
Ordered Locus Names:YJL194W
ORF Names:J0347
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL194W.
SGDiS000003730. CDC6.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: UniProtKB-SubCell
  • DNA replication preinitiation complex Source: SGD
  • nuclear pre-replicative complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi29K → R or T: Impairs nuclear localization. 1 Publication1
Mutagenesisi114K → E: Impairs ORC1-binding and leads to defective association with chromatin. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001509771 – 513Cell division control protein 6Add BLAST513

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei368PhosphothreonineCombined sources1

Post-translational modificationi

Ubiquitinated by the E3 ubiquitin ligase complex SCF(CDC4), DIA2, and TOM1; and targeted to the 26S proteasome for degradation.
Phosphorylated by CDC28. Phosphorylation is a prerequisite to ubiquitination and degradation.2 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP09119.

PTM databases

iPTMnetiP09119.

Expressioni

Inductioni

Transcribed at the end of mitosis, but in cells with a prolonged G1 phase there is a second burst of transcription in late G1.2 Publications

Interactioni

Subunit structurei

Associates with the ORC complex and the MCM2-7 helicase complex. Interacts with CDC4, CDC28, DIA2, ORC1, and TOM1.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33569. 297 interactors.
DIPiDIP-2267N.
IntActiP09119. 12 interactors.
MINTiMINT-469050.
STRINGi4932.YJL194W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5UDBelectron microscopy3.9091-513[»]
5V8Felectron microscopy3.9091-513[»]
ProteinModelPortaliP09119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 47Interaction with CDC4; required for degradation1 PublicationAdd BLAST47

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi27 – 33Nuclear localization signal1 Publication7

Sequence similaritiesi

Belongs to the CDC6/cdc18 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063498.
HOGENOMiHOG000057140.
InParanoidiP09119.
KOiK02213.
OMAiRKAFEIC.
OrthoDBiEOG092C0XFO.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR016314. Cdc6/18.
IPR015163. Cdc6_C.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF13401. AAA_22. 1 hit.
PF09079. Cdc6_C. 1 hit.
PIRSFiPIRSF001767. Cdc6. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P09119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIPITPTK RIRRNLFDDA PATPPRPLKR KKLQFTDVTP ESSPEKLQFG 
        60         70         80         90        100
SQSIFLRTKA LLQKSSELVN LNSSDGALPA RTAEYEQVMN FLAKAISEHR 
       110        120        130        140        150
SDSLYITGPP GTGKTAQLDM IIRQKFQSLP LSLSTPRSKD VLRHTNPNLQ 
       160        170        180        190        200
NLSWFELPDG RLESVAVTSI NCISLGEPSS IFQKIFDSFQ DLNGPTLQIK 
       210        220        230        240        250
NMQHLQKFLE PYHKKTTFVV VLDEMDRLLH ANTSETQSVR TILELFLLAK 
       260        270        280        290        300
LPTVSFVLIG MANSLDMKDR FLSRLNLDRG LLPQTIVFQP YTAEQMYEIV 
       310        320        330        340        350
IQKMSSLPTI IFQPMAIKFA AKKCAGNTGD LRKLFDVLRG SIEIYELEKR 
       360        370        380        390        400
FLLSPTRGSL NSAQVPLTPT TSPVKKSYPE PQGKIGLNYI AKVFSKFVNN 
       410        420        430        440        450
NSTRTRIAKL NIQQKLILCT IIQSLKLNSD ATIDESFDHY IKAITKTDTL 
       460        470        480        490        500
APLQRNEFLE ICTILETCGL VSIKKTKCKG KTKRFVDKID VDLDMREFYD 
       510 
EMTKISILKP FLH
Length:513
Mass (Da):58,037
Last modified:February 1, 1995 - v3
Checksum:i13645F65BEAB5EE8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3A → G in AAA34484 (PubMed:2656692).Curated1
Sequence conflicti202M → I in AAA34484 (PubMed:2656692).Curated1
Sequence conflicti225M → I in AAA34484 (PubMed:2656692).Curated1
Sequence conflicti272L → P in CAA31510 (PubMed:3062576).Curated1
Sequence conflicti277L → F in AAA34484 (PubMed:2656692).Curated1
Sequence conflicti339R → S in AAA34484 (PubMed:2656692).Curated1
Sequence conflicti395 – 396SK → LQN in CAA31510 (PubMed:3062576).Curated2
Sequence conflicti402S → L in CAA31510 (PubMed:3062576).Curated1
Sequence conflicti410L → S in CAA31510 (PubMed:3062576).Curated1
Sequence conflicti436S → L in CAA31510 (PubMed:3062576).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04734 Genomic DNA. Translation: AAA34484.1.
M22858 Genomic DNA. Translation: AAA34483.1.
X65299 Genomic DNA. Translation: CAA46392.1.
X77688 Genomic DNA. Translation: CAA54766.1.
Z49470 Genomic DNA. Translation: CAA89490.1.
M61183 Genomic DNA. Translation: AAA34488.1.
X13118 Genomic DNA. Translation: CAA31510.1.
BK006943 Genomic DNA. Translation: DAA08613.1.
PIRiS46640. RGBYC6.
RefSeqiNP_012341.1. NM_001181627.1.

Genome annotation databases

EnsemblFungiiYJL194W; YJL194W; YJL194W.
GeneIDi853244.
KEGGisce:YJL194W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCDC6_YEAST
AccessioniPrimary (citable) accession number: P09119
Secondary accession number(s): D6VVZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1995
Last modified: July 5, 2017
This is version 156 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names