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P09119 (CDC6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell division control protein 6
Gene names
Name:CDC6
Ordered Locus Names:YJL194W
ORF Names:J0347
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Appears to have an important and perhaps unique dual role in S phase, it is first required for the initiation of DNA replication and then actively participates in the suppression of nuclear division. Interacts with the origin recognition complex (ORC). Targeted for degradation by the E3 ubiquitin ligase complex SCF(Cdc4). Ref.3 Ref.9

Subunit structure

Interacts with CDC4. Ref.9

Subcellular location

Cell membrane.

Sequence similarities

Belongs to the CDC6/cdc18 family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
DNA replication
   Cellular componentCell membrane
Membrane
   LigandATP-binding
Nucleotide-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from direct assay PubMed 8083240. Source: GOC

G1/S transition of mitotic cell cycle

Inferred from mutant phenotype PubMed 10075735. Source: SGD

GTP catabolic process

Inferred from direct assay PubMed 8083240. Source: GOC

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

pre-replicative complex assembly involved in nuclear cell cycle DNA replication

Inferred from direct assay PubMed 16824194. Source: SGD

regulation of DNA-dependent DNA replication initiation

Inferred from mutant phenotype PubMed 9990508. Source: SGD

regulation of chromatin silencing at telomere

Inferred from mutant phenotype PubMed 16980387. Source: SGD

   Cellular_componentDNA replication preinitiation complex

Inferred from direct assay PubMed 9554851. Source: SGD

nuclear pre-replicative complex

Inferred from direct assay PubMed 16824194. Source: SGD

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from direct assay PubMed 8083240. Source: SGD

ATPase activity

Inferred from direct assay PubMed 8083240. Source: SGD

DNA replication origin binding

Inferred from direct assay PubMed 16824194PubMed 9288745. Source: SGD

GTP binding

Inferred from direct assay PubMed 8083240. Source: SGD

GTPase activity

Inferred from direct assay PubMed 8083240. Source: SGD

chromatin binding

Inferred from direct assay PubMed 9159120PubMed 9288745. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDC28P005462EBI-4447,EBI-4253
LSB3P436032EBI-4447,EBI-22980

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 513513Cell division control protein 6
PRO_0000150977

Regions

Nucleotide binding108 – 1158ATP Potential
Region1 – 4747Interaction with CDC4; required for degradation

Amino acid modifications

Modified residue3681Phosphothreonine Ref.10

Experimental info

Sequence conflict31A → G in AAA34484. Ref.1
Sequence conflict2721L → P in CAA31510. Ref.8
Sequence conflict395 – 3962SK → LQN in CAA31510. Ref.8
Sequence conflict4021S → L in CAA31510. Ref.8
Sequence conflict4101L → S in CAA31510. Ref.8
Sequence conflict4361S → L in CAA31510. Ref.8

Sequences

Sequence LengthMass (Da)Tools
P09119 [UniParc].

Last modified February 1, 1995. Version 3.
Checksum: 13645F65BEAB5EE8

FASTA51358,037
        10         20         30         40         50         60 
MSAIPITPTK RIRRNLFDDA PATPPRPLKR KKLQFTDVTP ESSPEKLQFG SQSIFLRTKA 

        70         80         90        100        110        120 
LLQKSSELVN LNSSDGALPA RTAEYEQVMN FLAKAISEHR SDSLYITGPP GTGKTAQLDM 

       130        140        150        160        170        180 
IIRQKFQSLP LSLSTPRSKD VLRHTNPNLQ NLSWFELPDG RLESVAVTSI NCISLGEPSS 

       190        200        210        220        230        240 
IFQKIFDSFQ DLNGPTLQIK NMQHLQKFLE PYHKKTTFVV VLDEMDRLLH ANTSETQSVR 

       250        260        270        280        290        300 
TILELFLLAK LPTVSFVLIG MANSLDMKDR FLSRLNLDRG LLPQTIVFQP YTAEQMYEIV 

       310        320        330        340        350        360 
IQKMSSLPTI IFQPMAIKFA AKKCAGNTGD LRKLFDVLRG SIEIYELEKR FLLSPTRGSL 

       370        380        390        400        410        420 
NSAQVPLTPT TSPVKKSYPE PQGKIGLNYI AKVFSKFVNN NSTRTRIAKL NIQQKLILCT 

       430        440        450        460        470        480 
IIQSLKLNSD ATIDESFDHY IKAITKTDTL APLQRNEFLE ICTILETCGL VSIKKTKCKG 

       490        500        510 
KTKRFVDKID VDLDMREFYD EMTKISILKP FLH 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of Saccharomyces cerevisiae CDC6 gene. Isolation, identification, and sequence analysis."
Zhou C., Huang S.H., Jong A.Y.
J. Biol. Chem. 264:9022-9029(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular cloning and characterization of the cell division cycle gene CDC6 from Saccharomyces cerevisiae."
Colasanti J.J., Comer A.R., Bruschi C.V.
Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division."
Bueno A., Russell P.
EMBO J. 11:2167-2176(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[4]"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes."
Purnelle B., Coster F., Goffeau A.
Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"CDC6 mRNA fluctuates periodically in the yeast cell cycle."
Zhou C., Jong A.Y.
J. Biol. Chem. 265:19904-19909(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
[8]"Cloning and characterization of the Saccharomyces cerevisiae CDC6 gene."
Lisziewicz J., Godany A., Agoston D.V., Kuentzel H.
Nucleic Acids Res. 16:11507-11520(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-513.
[9]"The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast."
Drury L.S., Perkins G., Diffley J.F.
EMBO J. 16:5966-5976(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDC4.
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-368, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04734 Genomic DNA. Translation: AAA34484.1. Sequence problems.
M22858 Genomic DNA. Translation: AAA34483.1.
X65299 Genomic DNA. Translation: CAA46392.1.
X77688 Genomic DNA. Translation: CAA54766.1.
Z49470 Genomic DNA. Translation: CAA89490.1.
M61183 Genomic DNA. Translation: AAA34488.1.
X13118 Genomic DNA. Translation: CAA31510.1.
BK006943 Genomic DNA. Translation: DAA08613.1.
PIRRGBYC6. S46640.
RefSeqNP_012341.1. NM_001181627.1.

3D structure databases

ProteinModelPortalP09119.
SMRP09119. Positions 82-128.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33569. 122 interactions.
DIPDIP-2267N.
IntActP09119. 12 interactions.
MINTMINT-469050.
STRING4932.YJL194W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL194W; YJL194W; YJL194W.
GeneID853244.
KEGGsce:YJL194W.

Organism-specific databases

CYGDYJL194w.
SGDS000003730. CDC6.

Phylogenomic databases

eggNOGCOG1474.
GeneTreeENSGT00530000063498.
HOGENOMHOG000057140.
KOK02213.
OMACCKKSAS.
OrthoDBEOG7ZWD9N.

Enzyme and pathway databases

BioCycYEAST:G3O-31626-MONOMER.

Gene expression databases

GenevestigatorP09119.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR016314. Cell_div_Cdc6/18.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PIRSFPIRSF001767. Cdc6. 1 hit.
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

NextBio973478.
PROP09119.

Entry information

Entry nameCDC6_YEAST
AccessionPrimary (citable) accession number: P09119
Secondary accession number(s): D6VVZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families