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P09119

- CDC6_YEAST

UniProt

P09119 - CDC6_YEAST

Protein

Cell division control protein 6

Gene

CDC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Plays a crucial role in forming the pre-replicative complexes. Interacts with the origin recognition complex (ORC) and MCM2-7 helicase complex leading to the linking of those complexes and loading of the replicative helicase MCM2-7 onto the pre-replicative complexes. Required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.9 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi108 – 1158ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: SGD
    3. chromatin binding Source: SGD
    4. DNA replication origin binding Source: SGD
    5. GTPase activity Source: SGD
    6. GTP binding Source: SGD
    7. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. cell division Source: UniProtKB-KW
    3. G1/S transition of mitotic cell cycle Source: SGD
    4. GTP catabolic process Source: GOC
    5. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
    6. regulation of chromatin silencing at telomere Source: SGD
    7. regulation of DNA-dependent DNA replication initiation Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31626-MONOMER.
    ReactomeiREACT_189247. G1/S-Specific Transcription.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division control protein 6
    Gene namesi
    Name:CDC6
    Ordered Locus Names:YJL194W
    ORF Names:J0347
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL194w.
    SGDiS000003730. CDC6.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: UniProtKB-SubCell
    2. DNA replication preinitiation complex Source: SGD
    3. nuclear pre-replicative complex Source: SGD

    Keywords - Cellular componenti

    Chromosome, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi29 – 291K → R or T: Impairs nuclear localization. 1 Publication
    Mutagenesisi114 – 1141K → E: Impairs ORC1-binding and leads to defective association with chromatin. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 513513Cell division control protein 6PRO_0000150977Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei368 – 3681Phosphothreonine1 Publication

    Post-translational modificationi

    Ubiquitinated by the E3 ubiquitin ligase complex SCF(CDC4), DIA2, and TOM1; and targeted to the 26S proteasome for degradation.
    Phosphorylated by CDC28. Phosphorylation is a prerequisite to ubiquitination and degradation.3 Publications

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Expressioni

    Inductioni

    Transcribed at the end of mitosis, but in cells with a prolonged G1 phase there is a second burst of transcription in late G1.2 Publications

    Gene expression databases

    GenevestigatoriP09119.

    Interactioni

    Subunit structurei

    Associates with the ORC complex and the MCM2-7 helicase complex. Interacts with CDC4, CDC28, DIA2, ORC1, and TOM1.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC28P005462EBI-4447,EBI-4253
    LSB3P436032EBI-4447,EBI-22980

    Protein-protein interaction databases

    BioGridi33569. 123 interactions.
    DIPiDIP-2267N.
    IntActiP09119. 12 interactions.
    MINTiMINT-469050.
    STRINGi4932.YJL194W.

    Structurei

    3D structure databases

    ProteinModelPortaliP09119.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4747Interaction with CDC4; required for degradationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi27 – 337Nuclear localization signal1 Publication

    Sequence similaritiesi

    Belongs to the CDC6/cdc18 family.Curated

    Phylogenomic databases

    eggNOGiCOG1474.
    GeneTreeiENSGT00530000063498.
    HOGENOMiHOG000057140.
    KOiK02213.
    OMAiANSLDMK.
    OrthoDBiEOG7ZWD9N.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR016314. Cell_div_Cdc6/18.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PIRSFiPIRSF001767. Cdc6. 1 hit.
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P09119-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAIPITPTK RIRRNLFDDA PATPPRPLKR KKLQFTDVTP ESSPEKLQFG    50
    SQSIFLRTKA LLQKSSELVN LNSSDGALPA RTAEYEQVMN FLAKAISEHR 100
    SDSLYITGPP GTGKTAQLDM IIRQKFQSLP LSLSTPRSKD VLRHTNPNLQ 150
    NLSWFELPDG RLESVAVTSI NCISLGEPSS IFQKIFDSFQ DLNGPTLQIK 200
    NMQHLQKFLE PYHKKTTFVV VLDEMDRLLH ANTSETQSVR TILELFLLAK 250
    LPTVSFVLIG MANSLDMKDR FLSRLNLDRG LLPQTIVFQP YTAEQMYEIV 300
    IQKMSSLPTI IFQPMAIKFA AKKCAGNTGD LRKLFDVLRG SIEIYELEKR 350
    FLLSPTRGSL NSAQVPLTPT TSPVKKSYPE PQGKIGLNYI AKVFSKFVNN 400
    NSTRTRIAKL NIQQKLILCT IIQSLKLNSD ATIDESFDHY IKAITKTDTL 450
    APLQRNEFLE ICTILETCGL VSIKKTKCKG KTKRFVDKID VDLDMREFYD 500
    EMTKISILKP FLH 513
    Length:513
    Mass (Da):58,037
    Last modified:February 1, 1995 - v3
    Checksum:i13645F65BEAB5EE8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31A → G in AAA34484. (PubMed:2656692)Curated
    Sequence conflicti202 – 2021M → I in AAA34484. (PubMed:2656692)Curated
    Sequence conflicti225 – 2251M → I in AAA34484. (PubMed:2656692)Curated
    Sequence conflicti272 – 2721L → P in CAA31510. (PubMed:3062576)Curated
    Sequence conflicti277 – 2771L → F in AAA34484. (PubMed:2656692)Curated
    Sequence conflicti339 – 3391R → S in AAA34484. (PubMed:2656692)Curated
    Sequence conflicti395 – 3962SK → LQN in CAA31510. (PubMed:3062576)Curated
    Sequence conflicti402 – 4021S → L in CAA31510. (PubMed:3062576)Curated
    Sequence conflicti410 – 4101L → S in CAA31510. (PubMed:3062576)Curated
    Sequence conflicti436 – 4361S → L in CAA31510. (PubMed:3062576)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04734 Genomic DNA. Translation: AAA34484.1.
    M22858 Genomic DNA. Translation: AAA34483.1.
    X65299 Genomic DNA. Translation: CAA46392.1.
    X77688 Genomic DNA. Translation: CAA54766.1.
    Z49470 Genomic DNA. Translation: CAA89490.1.
    M61183 Genomic DNA. Translation: AAA34488.1.
    X13118 Genomic DNA. Translation: CAA31510.1.
    BK006943 Genomic DNA. Translation: DAA08613.1.
    PIRiS46640. RGBYC6.
    RefSeqiNP_012341.1. NM_001181627.1.

    Genome annotation databases

    EnsemblFungiiYJL194W; YJL194W; YJL194W.
    GeneIDi853244.
    KEGGisce:YJL194W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04734 Genomic DNA. Translation: AAA34484.1 .
    M22858 Genomic DNA. Translation: AAA34483.1 .
    X65299 Genomic DNA. Translation: CAA46392.1 .
    X77688 Genomic DNA. Translation: CAA54766.1 .
    Z49470 Genomic DNA. Translation: CAA89490.1 .
    M61183 Genomic DNA. Translation: AAA34488.1 .
    X13118 Genomic DNA. Translation: CAA31510.1 .
    BK006943 Genomic DNA. Translation: DAA08613.1 .
    PIRi S46640. RGBYC6.
    RefSeqi NP_012341.1. NM_001181627.1.

    3D structure databases

    ProteinModelPortali P09119.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33569. 123 interactions.
    DIPi DIP-2267N.
    IntActi P09119. 12 interactions.
    MINTi MINT-469050.
    STRINGi 4932.YJL194W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL194W ; YJL194W ; YJL194W .
    GeneIDi 853244.
    KEGGi sce:YJL194W.

    Organism-specific databases

    CYGDi YJL194w.
    SGDi S000003730. CDC6.

    Phylogenomic databases

    eggNOGi COG1474.
    GeneTreei ENSGT00530000063498.
    HOGENOMi HOG000057140.
    KOi K02213.
    OMAi ANSLDMK.
    OrthoDBi EOG7ZWD9N.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31626-MONOMER.
    Reactomei REACT_189247. G1/S-Specific Transcription.

    Miscellaneous databases

    NextBioi 973478.
    PROi P09119.

    Gene expression databases

    Genevestigatori P09119.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR016314. Cell_div_Cdc6/18.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PIRSFi PIRSF001767. Cdc6. 1 hit.
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of Saccharomyces cerevisiae CDC6 gene. Isolation, identification, and sequence analysis."
      Zhou C., Huang S.H., Jong A.Y.
      J. Biol. Chem. 264:9022-9029(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Molecular cloning and characterization of the cell division cycle gene CDC6 from Saccharomyces cerevisiae."
      Colasanti J.J., Comer A.R., Bruschi C.V.
      Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division."
      Bueno A., Russell P.
      EMBO J. 11:2167-2176(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    4. "The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes."
      Purnelle B., Coster F., Goffeau A.
      Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. "CDC6 mRNA fluctuates periodically in the yeast cell cycle."
      Zhou C., Jong A.Y.
      J. Biol. Chem. 265:19904-19909(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
    8. "Cloning and characterization of the Saccharomyces cerevisiae CDC6 gene."
      Lisziewicz J., Godany A., Agoston D.V., Kuentzel H.
      Nucleic Acids Res. 16:11507-11520(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-513.
    9. "Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a 'reductional' anaphase in the budding yeast Saccharomyces cerevisiae."
      Piatti S., Lengauer C., Nasmyth K.
      EMBO J. 14:3788-3799(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, FUNCTION.
    10. "Intracellular location of the Saccharomyces cerevisiae CDC6 gene product."
      Jong A., Young M., Chen G.C., Zhang S.Q., Chan C.
      DNA Cell Biol. 15:883-895(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-29, NUCLEAR LOCALIZATION SIGNAL.
    11. "ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae."
      Santocanale C., Diffley J.F.
      EMBO J. 15:6671-6679(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast."
      Cocker J.H., Piatti S., Santocanale C., Nasmyth K., Diffley J.F.
      Nature 379:180-182(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, FUNCTION.
    13. "The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast."
      Drury L.S., Perkins G., Diffley J.F.
      EMBO J. 16:5966-5976(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC4.
    14. "Cdc6p establishes and maintains a state of replication competence during G1 phase."
      Detweiler C.S., Li J.J.
      J. Cell Sci. 110:753-763(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 association."
      Wang B., Feng L., Hu Y., Huang S.H., Reynolds C.P., Wu L., Jong A.Y.
      J. Biol. Chem. 274:8291-8298(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ORC1, MUTAGENESIS OF LYS-114, FUNCTION, SUBCELLULAR LOCATION.
    16. "Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis."
      Elsasser S., Chi Y., Yang P., Campbell J.L.
      Mol. Biol. Cell 10:3263-3277(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDC28, PHOSPHORYLATION BY CDC28, UBIQUITINATION.
    17. "The Cdc6p nucleotide-binding motif is required for loading mcm proteins onto chromatin."
      Weinreich M., Liang C., Stillman B.
      Proc. Natl. Acad. Sci. U.S.A. 96:441-446(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LYS-114, FUNCTION.
    18. "The stability of the Cdc6 protein is regulated by cyclin-dependent kinase/cyclin B complexes in Saccharomyces cerevisiae."
      Calzada A., Sanchez M., Sanchez E., Bueno A.
      J. Biol. Chem. 275:9734-9741(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDC28, PHOSPHORYLATION BY CDC28.
    19. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-368, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    20. "The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase."
      Kim D.H., Zhang W., Koepp D.M.
      J. Biol. Chem. 287:44212-44220(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DIA2 AND TOM1, UBIQUITINATION, SUBCELLULAR LOCATION.
    21. "An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly."
      Fernandez-Cid A., Riera A., Tognetti S., Herrera M.C., Samel S., Evrin C., Winkler C., Gardenal E., Uhle S., Speck C.
      Mol. Cell 50:577-588(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THE ORC AND MCM2-7 COMPLEXES, FUNCTION.

    Entry informationi

    Entry nameiCDC6_YEAST
    AccessioniPrimary (citable) accession number: P09119
    Secondary accession number(s): D6VVZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3