P09119 (CDC6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell division control protein 6

Gene names

Name:	CDC6
Ordered Locus Names:	YJL194W
ORF Names:	J0347

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to have an important and perhaps unique dual role in S phase, it is first required for the initiation of DNA replication and then actively participates in the suppression of nuclear division. Interacts with the origin recognition complex (ORC). Targeted for degradation by the E3 ubiquitin ligase complex SCF(Cdc4). Ref.3 Ref.9
Subunit structure	Interacts with CDC4. Ref.9
Subcellular location	Cell membrane.
Sequence similarities	Belongs to the CDC6/cdc18 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA replication
Cellular component	Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from mutant phenotype PubMed 10075735. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW pre-replicative complex assembly Inferred from direct assay PubMed 16824194. Source: SGD regulation of DNA-dependent DNA replication initiation Inferred from mutant phenotype PubMed 9990508. Source: SGD regulation of chromatin silencing at telomere Inferred from mutant phenotype PubMed 16980387. Source: SGD
Cellular_component	DNA replication preinitiation complex Inferred from direct assay PubMed 9554851. Source: SGD cytoplasm Inferred from direct assay PubMed 22842922. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell pre-replicative complex Inferred from direct assay PubMed 16824194. Source: SGD
Molecular_function	ATP binding Inferred from direct assay PubMed 8083240. Source: SGD ATPase activity Inferred from direct assay PubMed 8083240. Source: SGD DNA replication origin binding Inferred from direct assay PubMed 16824194PubMed 9288745. Source: SGD GTP binding Inferred from direct assay PubMed 8083240. Source: SGD GTPase activity Inferred from direct assay PubMed 8083240. Source: SGD chromatin binding Inferred from direct assay PubMed 9159120PubMed 9288745. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC28	P00546	2	EBI-4447,EBI-4253

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	Cell division control protein 6		PRO_0000150977
Regions
	Nucleotide binding	108 – 115	8	ATP Potential
	Region	1 – 47	47	Interaction with CDC4; required for degradation
Experimental info
	Sequence conflict	3	1	A → G in AAA34484. Ref.1
	Sequence conflict	272	1	L → P in CAA31510. Ref.8
	Sequence conflict	395 – 396	2	SK → LQN in CAA31510. Ref.8
	Sequence conflict	402	1	S → L in CAA31510. Ref.8
	Sequence conflict	410	1	L → S in CAA31510. Ref.8
	Sequence conflict	436	1	S → L in CAA31510. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P09119 [UniParc]. Last modified February 1, 1995. Version 3. Checksum: 13645F65BEAB5EE8	FASTA	513	58,037
	10 20 30 40 50 60 MSAIPITPTK RIRRNLFDDA PATPPRPLKR KKLQFTDVTP ESSPEKLQFG SQSIFLRTKA 70 80 90 100 110 120 LLQKSSELVN LNSSDGALPA RTAEYEQVMN FLAKAISEHR SDSLYITGPP GTGKTAQLDM 130 140 150 160 170 180 IIRQKFQSLP LSLSTPRSKD VLRHTNPNLQ NLSWFELPDG RLESVAVTSI NCISLGEPSS 190 200 210 220 230 240 IFQKIFDSFQ DLNGPTLQIK NMQHLQKFLE PYHKKTTFVV VLDEMDRLLH ANTSETQSVR 250 260 270 280 290 300 TILELFLLAK LPTVSFVLIG MANSLDMKDR FLSRLNLDRG LLPQTIVFQP YTAEQMYEIV 310 320 330 340 350 360 IQKMSSLPTI IFQPMAIKFA AKKCAGNTGD LRKLFDVLRG SIEIYELEKR FLLSPTRGSL 370 380 390 400 410 420 NSAQVPLTPT TSPVKKSYPE PQGKIGLNYI AKVFSKFVNN NSTRTRIAKL NIQQKLILCT 430 440 450 460 470 480 IIQSLKLNSD ATIDESFDHY IKAITKTDTL APLQRNEFLE ICTILETCGL VSIKKTKCKG 490 500 510 KTKRFVDKID VDLDMREFYD EMTKISILKP FLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of Saccharomyces cerevisiae CDC6 gene. Isolation, identification, and sequence analysis." Zhou C., Huang S.H., Jong A.Y. J. Biol. Chem. 264:9022-9029(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and characterization of the cell division cycle gene CDC6 from Saccharomyces cerevisiae." Colasanti J.J., Comer A.R., Bruschi C.V. Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division." Bueno A., Russell P. EMBO J. 11:2167-2176(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[4]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"CDC6 mRNA fluctuates periodically in the yeast cell cycle." Zhou C., Jong A.Y. J. Biol. Chem. 265:19904-19909(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
[8]	"Cloning and characterization of the Saccharomyces cerevisiae CDC6 gene." Lisziewicz J., Godany A., Agoston D.V., Kuentzel H. Nucleic Acids Res. 16:11507-11520(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-513.
[9]	"The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast." Drury L.S., Perkins G., Diffley J.F. EMBO J. 16:5966-5976(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04734 Genomic DNA. Translation: AAA34484.1. Sequence problems. M22858 Genomic DNA. Translation: AAA34483.1. X65299 Genomic DNA. Translation: CAA46392.1. X77688 Genomic DNA. Translation: CAA54766.1. Z49470 Genomic DNA. Translation: CAA89490.1. M61183 Genomic DNA. Translation: AAA34488.1. X13118 Genomic DNA. Translation: CAA31510.1. BK006943 Genomic DNA. Translation: DAA08613.1.
PIR	RGBYC6. S46640.
RefSeq	NP_012341.1. NM_001181627.1.
3D structure databases
ProteinModelPortal	P09119.
SMR	P09119. Positions 82-128.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2267N.
IntAct	P09119. 8 interactions.
MINT	MINT-469050.
STRING	4932.YJL194W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL194W; YJL194W; YJL194W.
GeneID	853244.
KEGG	sce:YJL194W.
Organism-specific databases
CYGD	YJL194w.
SGD	S000003730. CDC6.
Phylogenomic databases
eggNOG	COG1474.
GeneTree	ENSGT00530000063498.
HOGENOM	HOG000057140.
KO	K02213.
OMA	QQKAALC.
OrthoDB	EOG45QMND.
Gene expression databases
Genevestigator	P09119.
GermOnline	YJL194W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR016314. Cell_div_Cdc6/18. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PIRSF	PIRSF001767. Cdc6. 1 hit.
SMART	SM00382. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973478.

Entry information

Entry name

CDC6_YEAST

Accession

Primary (citable) accession number: P09119
Secondary accession number(s): D6VVZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 114 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program