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P09119

- CDC6_YEAST

UniProt

P09119 - CDC6_YEAST

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Protein

Cell division control protein 6

Gene

CDC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a crucial role in forming the pre-replicative complexes. Interacts with the origin recognition complex (ORC) and MCM2-7 helicase complex leading to the linking of those complexes and loading of the replicative helicase MCM2-7 onto the pre-replicative complexes. Required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.9 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1158ATPSequence Analysis

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. ATP binding Source: SGD
  3. chromatin binding Source: SGD
  4. DNA replication origin binding Source: SGD
  5. GTPase activity Source: SGD
  6. GTP binding Source: SGD

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. cell division Source: UniProtKB-KW
  3. G1/S transition of mitotic cell cycle Source: SGD
  4. GTP catabolic process Source: GOC
  5. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
  6. regulation of chromatin silencing at telomere Source: SGD
  7. regulation of DNA-dependent DNA replication initiation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31626-MONOMER.
ReactomeiREACT_189247. G1/S-Specific Transcription.
REACT_237144. Association of licensing factors with the pre-replicative complex.
REACT_245912. CDK-mediated phosphorylation and removal of Cdc6.
REACT_252897. E2F mediated regulation of DNA replication.
REACT_256856. CDC6 association with the ORC:origin complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 6
Gene namesi
Name:CDC6
Ordered Locus Names:YJL194W
ORF Names:J0347
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL194w.
SGDiS000003730. CDC6.

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: UniProtKB-KW
  2. DNA replication preinitiation complex Source: SGD
  3. nuclear pre-replicative complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi29 – 291K → R or T: Impairs nuclear localization. 1 Publication
Mutagenesisi114 – 1141K → E: Impairs ORC1-binding and leads to defective association with chromatin. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513Cell division control protein 6PRO_0000150977Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei368 – 3681Phosphothreonine1 Publication

Post-translational modificationi

Ubiquitinated by the E3 ubiquitin ligase complex SCF(CDC4), DIA2, and TOM1; and targeted to the 26S proteasome for degradation.
Phosphorylated by CDC28. Phosphorylation is a prerequisite to ubiquitination and degradation.3 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Expressioni

Inductioni

Transcribed at the end of mitosis, but in cells with a prolonged G1 phase there is a second burst of transcription in late G1.2 Publications

Gene expression databases

GenevestigatoriP09119.

Interactioni

Subunit structurei

Associates with the ORC complex and the MCM2-7 helicase complex. Interacts with CDC4, CDC28, DIA2, ORC1, and TOM1.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC28P005462EBI-4447,EBI-4253
LSB3P436032EBI-4447,EBI-22980

Protein-protein interaction databases

BioGridi33569. 123 interactions.
DIPiDIP-2267N.
IntActiP09119. 12 interactions.
MINTiMINT-469050.
STRINGi4932.YJL194W.

Structurei

3D structure databases

ProteinModelPortaliP09119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4747Interaction with CDC4; required for degradationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi27 – 337Nuclear localization signal1 Publication

Sequence similaritiesi

Belongs to the CDC6/cdc18 family.Curated

Phylogenomic databases

eggNOGiCOG1474.
GeneTreeiENSGT00530000063498.
HOGENOMiHOG000057140.
InParanoidiP09119.
KOiK02213.
OMAiANSLDMK.
OrthoDBiEOG7ZWD9N.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR016314. Cell_div_Cdc6/18.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PIRSFiPIRSF001767. Cdc6. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P09119-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAIPITPTK RIRRNLFDDA PATPPRPLKR KKLQFTDVTP ESSPEKLQFG
60 70 80 90 100
SQSIFLRTKA LLQKSSELVN LNSSDGALPA RTAEYEQVMN FLAKAISEHR
110 120 130 140 150
SDSLYITGPP GTGKTAQLDM IIRQKFQSLP LSLSTPRSKD VLRHTNPNLQ
160 170 180 190 200
NLSWFELPDG RLESVAVTSI NCISLGEPSS IFQKIFDSFQ DLNGPTLQIK
210 220 230 240 250
NMQHLQKFLE PYHKKTTFVV VLDEMDRLLH ANTSETQSVR TILELFLLAK
260 270 280 290 300
LPTVSFVLIG MANSLDMKDR FLSRLNLDRG LLPQTIVFQP YTAEQMYEIV
310 320 330 340 350
IQKMSSLPTI IFQPMAIKFA AKKCAGNTGD LRKLFDVLRG SIEIYELEKR
360 370 380 390 400
FLLSPTRGSL NSAQVPLTPT TSPVKKSYPE PQGKIGLNYI AKVFSKFVNN
410 420 430 440 450
NSTRTRIAKL NIQQKLILCT IIQSLKLNSD ATIDESFDHY IKAITKTDTL
460 470 480 490 500
APLQRNEFLE ICTILETCGL VSIKKTKCKG KTKRFVDKID VDLDMREFYD
510
EMTKISILKP FLH
Length:513
Mass (Da):58,037
Last modified:February 1, 1995 - v3
Checksum:i13645F65BEAB5EE8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31A → G in AAA34484. (PubMed:2656692)Curated
Sequence conflicti202 – 2021M → I in AAA34484. (PubMed:2656692)Curated
Sequence conflicti225 – 2251M → I in AAA34484. (PubMed:2656692)Curated
Sequence conflicti272 – 2721L → P in CAA31510. (PubMed:3062576)Curated
Sequence conflicti277 – 2771L → F in AAA34484. (PubMed:2656692)Curated
Sequence conflicti339 – 3391R → S in AAA34484. (PubMed:2656692)Curated
Sequence conflicti395 – 3962SK → LQN in CAA31510. (PubMed:3062576)Curated
Sequence conflicti402 – 4021S → L in CAA31510. (PubMed:3062576)Curated
Sequence conflicti410 – 4101L → S in CAA31510. (PubMed:3062576)Curated
Sequence conflicti436 – 4361S → L in CAA31510. (PubMed:3062576)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04734 Genomic DNA. Translation: AAA34484.1.
M22858 Genomic DNA. Translation: AAA34483.1.
X65299 Genomic DNA. Translation: CAA46392.1.
X77688 Genomic DNA. Translation: CAA54766.1.
Z49470 Genomic DNA. Translation: CAA89490.1.
M61183 Genomic DNA. Translation: AAA34488.1.
X13118 Genomic DNA. Translation: CAA31510.1.
BK006943 Genomic DNA. Translation: DAA08613.1.
PIRiS46640. RGBYC6.
RefSeqiNP_012341.1. NM_001181627.1.

Genome annotation databases

EnsemblFungiiYJL194W; YJL194W; YJL194W.
GeneIDi853244.
KEGGisce:YJL194W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04734 Genomic DNA. Translation: AAA34484.1 .
M22858 Genomic DNA. Translation: AAA34483.1 .
X65299 Genomic DNA. Translation: CAA46392.1 .
X77688 Genomic DNA. Translation: CAA54766.1 .
Z49470 Genomic DNA. Translation: CAA89490.1 .
M61183 Genomic DNA. Translation: AAA34488.1 .
X13118 Genomic DNA. Translation: CAA31510.1 .
BK006943 Genomic DNA. Translation: DAA08613.1 .
PIRi S46640. RGBYC6.
RefSeqi NP_012341.1. NM_001181627.1.

3D structure databases

ProteinModelPortali P09119.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33569. 123 interactions.
DIPi DIP-2267N.
IntActi P09119. 12 interactions.
MINTi MINT-469050.
STRINGi 4932.YJL194W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL194W ; YJL194W ; YJL194W .
GeneIDi 853244.
KEGGi sce:YJL194W.

Organism-specific databases

CYGDi YJL194w.
SGDi S000003730. CDC6.

Phylogenomic databases

eggNOGi COG1474.
GeneTreei ENSGT00530000063498.
HOGENOMi HOG000057140.
InParanoidi P09119.
KOi K02213.
OMAi ANSLDMK.
OrthoDBi EOG7ZWD9N.

Enzyme and pathway databases

BioCyci YEAST:G3O-31626-MONOMER.
Reactomei REACT_189247. G1/S-Specific Transcription.
REACT_237144. Association of licensing factors with the pre-replicative complex.
REACT_245912. CDK-mediated phosphorylation and removal of Cdc6.
REACT_252897. E2F mediated regulation of DNA replication.
REACT_256856. CDC6 association with the ORC:origin complex.

Miscellaneous databases

NextBioi 973478.
PROi P09119.

Gene expression databases

Genevestigatori P09119.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR016314. Cell_div_Cdc6/18.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PIRSFi PIRSF001767. Cdc6. 1 hit.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of Saccharomyces cerevisiae CDC6 gene. Isolation, identification, and sequence analysis."
    Zhou C., Huang S.H., Jong A.Y.
    J. Biol. Chem. 264:9022-9029(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning and characterization of the cell division cycle gene CDC6 from Saccharomyces cerevisiae."
    Colasanti J.J., Comer A.R., Bruschi C.V.
    Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division."
    Bueno A., Russell P.
    EMBO J. 11:2167-2176(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  4. "The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes."
    Purnelle B., Coster F., Goffeau A.
    Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "CDC6 mRNA fluctuates periodically in the yeast cell cycle."
    Zhou C., Jong A.Y.
    J. Biol. Chem. 265:19904-19909(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
  8. "Cloning and characterization of the Saccharomyces cerevisiae CDC6 gene."
    Lisziewicz J., Godany A., Agoston D.V., Kuentzel H.
    Nucleic Acids Res. 16:11507-11520(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-513.
  9. "Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a 'reductional' anaphase in the budding yeast Saccharomyces cerevisiae."
    Piatti S., Lengauer C., Nasmyth K.
    EMBO J. 14:3788-3799(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, FUNCTION.
  10. "Intracellular location of the Saccharomyces cerevisiae CDC6 gene product."
    Jong A., Young M., Chen G.C., Zhang S.Q., Chan C.
    DNA Cell Biol. 15:883-895(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-29, NUCLEAR LOCALIZATION SIGNAL.
  11. "ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae."
    Santocanale C., Diffley J.F.
    EMBO J. 15:6671-6679(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast."
    Cocker J.H., Piatti S., Santocanale C., Nasmyth K., Diffley J.F.
    Nature 379:180-182(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, FUNCTION.
  13. "The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast."
    Drury L.S., Perkins G., Diffley J.F.
    EMBO J. 16:5966-5976(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC4.
  14. "Cdc6p establishes and maintains a state of replication competence during G1 phase."
    Detweiler C.S., Li J.J.
    J. Cell Sci. 110:753-763(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 association."
    Wang B., Feng L., Hu Y., Huang S.H., Reynolds C.P., Wu L., Jong A.Y.
    J. Biol. Chem. 274:8291-8298(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ORC1, MUTAGENESIS OF LYS-114, FUNCTION, SUBCELLULAR LOCATION.
  16. "Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis."
    Elsasser S., Chi Y., Yang P., Campbell J.L.
    Mol. Biol. Cell 10:3263-3277(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC28, PHOSPHORYLATION BY CDC28, UBIQUITINATION.
  17. "The Cdc6p nucleotide-binding motif is required for loading mcm proteins onto chromatin."
    Weinreich M., Liang C., Stillman B.
    Proc. Natl. Acad. Sci. U.S.A. 96:441-446(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-114, FUNCTION.
  18. "The stability of the Cdc6 protein is regulated by cyclin-dependent kinase/cyclin B complexes in Saccharomyces cerevisiae."
    Calzada A., Sanchez M., Sanchez E., Bueno A.
    J. Biol. Chem. 275:9734-9741(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC28, PHOSPHORYLATION BY CDC28.
  19. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-368, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  20. "The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase."
    Kim D.H., Zhang W., Koepp D.M.
    J. Biol. Chem. 287:44212-44220(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DIA2 AND TOM1, UBIQUITINATION, SUBCELLULAR LOCATION.
  21. "An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly."
    Fernandez-Cid A., Riera A., Tognetti S., Herrera M.C., Samel S., Evrin C., Winkler C., Gardenal E., Uhle S., Speck C.
    Mol. Cell 50:577-588(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THE ORC AND MCM2-7 COMPLEXES, FUNCTION.

Entry informationi

Entry nameiCDC6_YEAST
AccessioniPrimary (citable) accession number: P09119
Secondary accession number(s): D6VVZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3