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Protein

Uricase

Gene

Uox

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.1 Publication

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.1 Publication

Enzyme regulationi

Competitively inhibited by xanthine.1 Publication

Kineticsi

  1. KM=8.33 µM for urate1 Publication

    Pathwayi: urate degradation

    This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate.1 Publication
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Uricase (Uox)
    2. no protein annotated in this organism
    3. no protein annotated in this organism
    This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei23Charge relay systemBy similarity1
    Active sitei68Charge relay systemBy similarity1
    Binding sitei170SubstrateBy similarity1
    Binding sitei187SubstrateBy similarity1
    Active sitei263Charge relay systemBy similarity1

    GO - Molecular functioni

    • urate oxidase activity Source: UniProtKB

    GO - Biological processi

    • purine nucleobase catabolic process Source: RGD
    • urate catabolic process Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    Biological processPurine metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00394; UER00650.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uricase (EC:1.7.3.31 Publication)
    Alternative name(s):
    Urate oxidase
    Gene namesi
    Name:Uox
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 2

    Organism-specific databases

    RGDi621369. Uox.

    Subcellular locationi

    GO - Cellular componenti

    • mitochondrion Source: Ensembl
    • peroxisomal matrix Source: RGD
    • peroxisome Source: UniProtKB

    Keywords - Cellular componenti

    Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi164K → E or I: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedBy similarity
    ChainiPRO_00001659902 – 303UricaseAdd BLAST302

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylalanineBy similarity1
    Modified residuei10N6-acetyllysine; alternateBy similarity1
    Modified residuei10N6-succinyllysine; alternateBy similarity1
    Modified residuei23N6-acetyllysine; alternateBy similarity1
    Modified residuei23N6-succinyllysine; alternateBy similarity1
    Modified residuei27N6-acetyllysineBy similarity1
    Modified residuei36N6-acetyllysineBy similarity1
    Modified residuei39PhosphoserineBy similarity1
    Modified residuei63PhosphoserineBy similarity1
    Modified residuei118N6-acetyllysineBy similarity1
    Modified residuei122N6-acetyllysineBy similarity1
    Modified residuei164N6-acetyllysineBy similarity1
    Modified residuei175N6-acetyllysineBy similarity1
    Modified residuei185N6-acetyllysineBy similarity1
    Modified residuei220N6-acetyllysine; alternateBy similarity1
    Modified residuei220N6-succinyllysine; alternateBy similarity1
    Modified residuei231PhosphoserineBy similarity1
    Modified residuei277N6-acetyllysineBy similarity1
    Modified residuei288PhosphotyrosineCombined sources1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP09118.
    PRIDEiP09118.

    PTM databases

    iPTMnetiP09118.
    PhosphoSitePlusiP09118.

    Expressioni

    Tissue specificityi

    Expressed in liver. Not detected in other tissues tested.1 Publication

    Gene expression databases

    BgeeiENSRNOG00000016339.
    GenevisibleiP09118. RN.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4586902.
    STRINGi10116.ENSRNOP00000021970.

    Structurei

    3D structure databases

    ProteinModelPortaliP09118.
    SMRiP09118.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni68 – 69Substrate bindingBy similarity2
    Regioni234 – 235Substrate bindingBy similarity2

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi301 – 303Microbody targeting signalSequence analysis3

    Sequence similaritiesi

    Belongs to the uricase family.Curated

    Phylogenomic databases

    eggNOGiKOG1599. Eukaryota.
    COG3648. LUCA.
    GeneTreeiENSGT00390000010863.
    HOGENOMiHOG000250659.
    HOVERGENiHBG018060.
    InParanoidiP09118.
    KOiK00365.
    OMAiKDRCFAT.
    OrthoDBiEOG091G0E99.
    PhylomeDBiP09118.
    TreeFamiTF323438.

    Family and domain databases

    InterProiView protein in InterPro
    IPR002042. Uricase.
    IPR019842. Uricase_CS.
    PfamiView protein in Pfam
    PF01014. Uricase. 2 hits.
    PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
    PRINTSiPR00093. URICASE.
    TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
    PROSITEiView protein in PROSITE
    PS00366. URICASE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09118-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAHYHDDYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT
    60 70 80 90 100
    LRSKKDYLHG DNSDIIPTDT IKNTVHVLAK FKGIKSIETF AMNICEHFLS
    110 120 130 140 150
    SFSHVTRAHV YVEEVPWKRF EKNGVKHVHA FIHTPTGTHF CDVEQVRNGP
    160 170 180 190 200
    PIIHSGIKDL KVLKTTQSGF EGFIKDQFTT LPEVKDRCFA TQVYCKWRYQ
    210 220 230 240 250
    NRDVDFEATW GAVRDIVLKK FAGPYDRGEY SPSVQKTLYD IQVLTLSQLP
    260 270 280 290 300
    EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVRRKLP

    SRL
    Length:303
    Mass (Da):34,934
    Last modified:January 23, 2007 - v3
    Checksum:i14CFF2F4E18C7BBB
    GO

    Sequence cautioni

    The sequence AAA42318 differs from that shown. Reason: Frameshift at position 288.Curated
    The sequence CAA30378 differs from that shown. Reason: Frameshift at several positions.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti55K → D (PubMed:1783398).Curated1
    Sequence conflicti111Y → H in AAA42317 (PubMed:2920046).Curated1
    Sequence conflicti111Y → H (PubMed:1783398).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J03959 mRNA. Translation: AAA42318.1. Frameshift.
    X13098 mRNA. Translation: CAA31490.1.
    M24396 mRNA. Translation: AAA42317.1.
    X07497 mRNA. Translation: CAA30378.1. Frameshift.
    M63593
    , M63583, M63586, M63587, M63588, M63590, M63591, M63592 Genomic DNA. Translation: AAA61699.1.
    BC088112 mRNA. Translation: AAH88112.1.
    PIRiI54070. RDRTU.
    RefSeqiNP_446220.1. NM_053768.2.
    UniGeneiRn.11330.

    Genome annotation databases

    EnsembliENSRNOT00000021970; ENSRNOP00000021970; ENSRNOG00000016339.
    GeneIDi114768.
    KEGGirno:114768.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiURIC_RAT
    AccessioniPrimary (citable) accession number: P09118
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: January 23, 2007
    Last modified: June 7, 2017
    This is version 155 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families