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Protein

Uricase

Gene

Uox

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.

Pathwayi: urate degradation

This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (Uox)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei187Charge relay systemBy similarity1
Binding sitei187SubstrateBy similarity1
Active sitei235Charge relay systemBy similarity1

GO - Molecular functioni

  • urate oxidase activity Source: RGD

GO - Biological processi

  • allantoin biosynthetic process Source: GO_Central
  • purine nucleobase catabolic process Source: RGD
  • urate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Names & Taxonomyi

Protein namesi
Recommended name:
Uricase (EC:1.7.3.3)
Alternative name(s):
Urate oxidase
Gene namesi
Name:Uox
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi621369. Uox.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: Ensembl
  • peroxisomal matrix Source: RGD
  • peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi164K → E or I: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001659902 – 303UricaseAdd BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei10N6-acetyllysine; alternateBy similarity1
Modified residuei10N6-succinyllysine; alternateBy similarity1
Modified residuei23N6-acetyllysine; alternateBy similarity1
Modified residuei23N6-succinyllysine; alternateBy similarity1
Modified residuei27N6-acetyllysineBy similarity1
Modified residuei36N6-acetyllysineBy similarity1
Modified residuei39PhosphoserineBy similarity1
Modified residuei63PhosphoserineBy similarity1
Modified residuei118N6-acetyllysineBy similarity1
Modified residuei122N6-acetyllysineBy similarity1
Modified residuei164N6-acetyllysineBy similarity1
Modified residuei175N6-acetyllysineBy similarity1
Modified residuei185N6-acetyllysineBy similarity1
Modified residuei220N6-acetyllysine; alternateBy similarity1
Modified residuei220N6-succinyllysine; alternateBy similarity1
Modified residuei231PhosphoserineBy similarity1
Modified residuei277N6-acetyllysineBy similarity1
Modified residuei288PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP09118.
PRIDEiP09118.

PTM databases

iPTMnetiP09118.
PhosphoSitePlusiP09118.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016339.
GenevisibleiP09118. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4586902.
STRINGi10116.ENSRNOP00000021970.

Structurei

3D structure databases

ProteinModelPortaliP09118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 69Substrate bindingBy similarity2
Regioni234 – 235Substrate bindingBy similarity2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi301 – 303Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the uricase family.Curated

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
GeneTreeiENSGT00390000010863.
HOGENOMiHOG000250659.
HOVERGENiHBG018060.
InParanoidiP09118.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG091G0E99.
PhylomeDBiP09118.
TreeFamiTF323438.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHYHDDYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT
60 70 80 90 100
LRSKKDYLHG DNSDIIPTDT IKNTVHVLAK FKGIKSIETF AMNICEHFLS
110 120 130 140 150
SFSHVTRAHV YVEEVPWKRF EKNGVKHVHA FIHTPTGTHF CDVEQVRNGP
160 170 180 190 200
PIIHSGIKDL KVLKTTQSGF EGFIKDQFTT LPEVKDRCFA TQVYCKWRYQ
210 220 230 240 250
NRDVDFEATW GAVRDIVLKK FAGPYDRGEY SPSVQKTLYD IQVLTLSQLP
260 270 280 290 300
EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVRRKLP

SRL
Length:303
Mass (Da):34,934
Last modified:January 23, 2007 - v3
Checksum:i14CFF2F4E18C7BBB
GO

Sequence cautioni

The sequence AAA42318 differs from that shown. Reason: Frameshift at position 288.Curated
The sequence CAA30378 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55K → D (PubMed:1783398).Curated1
Sequence conflicti111Y → H in AAA42317 (PubMed:2920046).Curated1
Sequence conflicti111Y → H (PubMed:1783398).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03959 mRNA. Translation: AAA42318.1. Frameshift.
X13098 mRNA. Translation: CAA31490.1.
M24396 mRNA. Translation: AAA42317.1.
X07497 mRNA. Translation: CAA30378.1. Frameshift.
M63593
, M63583, M63586, M63587, M63588, M63590, M63591, M63592 Genomic DNA. Translation: AAA61699.1.
BC088112 mRNA. Translation: AAH88112.1.
PIRiI54070. RDRTU.
RefSeqiNP_446220.1. NM_053768.2.
UniGeneiRn.11330.

Genome annotation databases

EnsembliENSRNOT00000021970; ENSRNOP00000021970; ENSRNOG00000016339.
GeneIDi114768.
KEGGirno:114768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03959 mRNA. Translation: AAA42318.1. Frameshift.
X13098 mRNA. Translation: CAA31490.1.
M24396 mRNA. Translation: AAA42317.1.
X07497 mRNA. Translation: CAA30378.1. Frameshift.
M63593
, M63583, M63586, M63587, M63588, M63590, M63591, M63592 Genomic DNA. Translation: AAA61699.1.
BC088112 mRNA. Translation: AAH88112.1.
PIRiI54070. RDRTU.
RefSeqiNP_446220.1. NM_053768.2.
UniGeneiRn.11330.

3D structure databases

ProteinModelPortaliP09118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4586902.
STRINGi10116.ENSRNOP00000021970.

PTM databases

iPTMnetiP09118.
PhosphoSitePlusiP09118.

Proteomic databases

PaxDbiP09118.
PRIDEiP09118.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021970; ENSRNOP00000021970; ENSRNOG00000016339.
GeneIDi114768.
KEGGirno:114768.

Organism-specific databases

CTDi391051.
RGDi621369. Uox.

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
GeneTreeiENSGT00390000010863.
HOGENOMiHOG000250659.
HOVERGENiHBG018060.
InParanoidiP09118.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG091G0E99.
PhylomeDBiP09118.
TreeFamiTF323438.

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Miscellaneous databases

PROiP09118.

Gene expression databases

BgeeiENSRNOG00000016339.
GenevisibleiP09118. RN.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURIC_RAT
AccessioniPrimary (citable) accession number: P09118
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.