Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Uricase

Gene

Uox

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.

Pathwayi: urate degradation

This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (Uox)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871Charge relay systemBy similarity
Binding sitei187 – 1871SubstrateBy similarity
Active sitei235 – 2351Charge relay systemBy similarity

GO - Molecular functioni

  • urate oxidase activity Source: RGD

GO - Biological processi

  • purine nucleobase catabolic process Source: RGD
  • urate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Names & Taxonomyi

Protein namesi
Recommended name:
Uricase (EC:1.7.3.3)
Alternative name(s):
Urate oxidase
Gene namesi
Name:Uox
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi621369. Uox.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: Ensembl
  • peroxisomal matrix Source: RGD
  • peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi164 – 1641K → E or I: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 303302UricasePRO_0000165990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei10 – 101N6-acetyllysine; alternateBy similarity
Modified residuei10 – 101N6-succinyllysine; alternateBy similarity
Modified residuei23 – 231N6-acetyllysine; alternateBy similarity
Modified residuei23 – 231N6-succinyllysine; alternateBy similarity
Modified residuei27 – 271N6-acetyllysineBy similarity
Modified residuei36 – 361N6-acetyllysineBy similarity
Modified residuei39 – 391PhosphoserineBy similarity
Modified residuei63 – 631PhosphoserineBy similarity
Modified residuei118 – 1181N6-acetyllysineBy similarity
Modified residuei122 – 1221N6-acetyllysineBy similarity
Modified residuei164 – 1641N6-acetyllysineBy similarity
Modified residuei175 – 1751N6-acetyllysineBy similarity
Modified residuei185 – 1851N6-acetyllysineBy similarity
Modified residuei220 – 2201N6-acetyllysine; alternateBy similarity
Modified residuei220 – 2201N6-succinyllysine; alternateBy similarity
Modified residuei231 – 2311PhosphoserineBy similarity
Modified residuei277 – 2771N6-acetyllysineBy similarity
Modified residuei288 – 2881PhosphotyrosineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP09118.
PRIDEiP09118.

PTM databases

iPTMnetiP09118.
PhosphoSiteiP09118.

Expressioni

Gene expression databases

GenevisibleiP09118. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4586902.
STRINGi10116.ENSRNOP00000021970.

Structurei

3D structure databases

ProteinModelPortaliP09118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 692Substrate bindingBy similarity
Regioni234 – 2352Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi301 – 3033Microbody targeting signalSequence analysis

Sequence similaritiesi

Belongs to the uricase family.Curated

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
GeneTreeiENSGT00390000010863.
HOGENOMiHOG000250659.
HOVERGENiHBG018060.
InParanoidiP09118.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG79KPFH.
PhylomeDBiP09118.
TreeFamiTF323438.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHYHDDYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT
60 70 80 90 100
LRSKKDYLHG DNSDIIPTDT IKNTVHVLAK FKGIKSIETF AMNICEHFLS
110 120 130 140 150
SFSHVTRAHV YVEEVPWKRF EKNGVKHVHA FIHTPTGTHF CDVEQVRNGP
160 170 180 190 200
PIIHSGIKDL KVLKTTQSGF EGFIKDQFTT LPEVKDRCFA TQVYCKWRYQ
210 220 230 240 250
NRDVDFEATW GAVRDIVLKK FAGPYDRGEY SPSVQKTLYD IQVLTLSQLP
260 270 280 290 300
EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVRRKLP

SRL
Length:303
Mass (Da):34,934
Last modified:January 23, 2007 - v3
Checksum:i14CFF2F4E18C7BBB
GO

Sequence cautioni

The sequence AAA42318.1 differs from that shown. Reason: Frameshift at position 288. Curated
The sequence CAA30378.1 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551K → D (PubMed:1783398).Curated
Sequence conflicti111 – 1111Y → H in AAA42317 (PubMed:2920046).Curated
Sequence conflicti111 – 1111Y → H (PubMed:1783398).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03959 mRNA. Translation: AAA42318.1. Frameshift.
X13098 mRNA. Translation: CAA31490.1.
M24396 mRNA. Translation: AAA42317.1.
X07497 mRNA. Translation: CAA30378.1. Frameshift.
M63593
, M63583, M63586, M63587, M63588, M63590, M63591, M63592 Genomic DNA. Translation: AAA61699.1.
BC088112 mRNA. Translation: AAH88112.1.
PIRiI54070. RDRTU.
RefSeqiNP_446220.1. NM_053768.2.
UniGeneiRn.11330.

Genome annotation databases

EnsembliENSRNOT00000021970; ENSRNOP00000021970; ENSRNOG00000016339.
GeneIDi114768.
KEGGirno:114768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03959 mRNA. Translation: AAA42318.1. Frameshift.
X13098 mRNA. Translation: CAA31490.1.
M24396 mRNA. Translation: AAA42317.1.
X07497 mRNA. Translation: CAA30378.1. Frameshift.
M63593
, M63583, M63586, M63587, M63588, M63590, M63591, M63592 Genomic DNA. Translation: AAA61699.1.
BC088112 mRNA. Translation: AAH88112.1.
PIRiI54070. RDRTU.
RefSeqiNP_446220.1. NM_053768.2.
UniGeneiRn.11330.

3D structure databases

ProteinModelPortaliP09118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4586902.
STRINGi10116.ENSRNOP00000021970.

PTM databases

iPTMnetiP09118.
PhosphoSiteiP09118.

Proteomic databases

PaxDbiP09118.
PRIDEiP09118.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021970; ENSRNOP00000021970; ENSRNOG00000016339.
GeneIDi114768.
KEGGirno:114768.

Organism-specific databases

CTDi391051.
RGDi621369. Uox.

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
GeneTreeiENSGT00390000010863.
HOGENOMiHOG000250659.
HOVERGENiHBG018060.
InParanoidiP09118.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG79KPFH.
PhylomeDBiP09118.
TreeFamiTF323438.

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Miscellaneous databases

PROiP09118.

Gene expression databases

GenevisibleiP09118. RN.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Organization of rat uricase chromosomal gene differs greatly from that of the corresponding plant gene."
    Motojima K., Goto S.
    FEBS Lett. 264:156-158(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Cloning and sequence analysis of cDNA for rat liver uricase."
    Motojima K., Kanaya S., Goto S.
    J. Biol. Chem. 263:16677-16681(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase."
    Alvares K., Nemali M.R., Reddy P.G., Wang X.D., Rao M.S., Reddy J.K.
    Biochem. Biophys. Res. Commun. 158:991-995(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  4. "Nucleotide sequence of cDNA and predicted amino acid sequence of rat liver uricase."
    Ito M., Suzuki M., Takagi Y.
    Eur. J. Biochem. 173:459-463(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  5. "Rat urate oxidase: cloning and structural analysis of the gene and 5'-flanking region."
    Wang X.D., Kawano H., Alvares K., Reddy P.G., Getto H., Rao M.S., Reddy J.K.
    Gene 97:223-229(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Structural analysis of the gene encoding rat uricase."
    Ito M., Nakamura M., Ogawa H., Kato S., Takagi Y.
    Genomics 11:905-913(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  8. "Isolation and sequence determination of a cDNA clone for rat peroxisomal urate oxidase: liver-specific expression in the rat."
    Reddy P.G., Nemali M.R., Reddy M.K., Reddy M.N., Yuan P.M., Yuen S., Laffler T.G., Shiroza T., Kuramitsu H.K., Usuda N., Chisholm R.L., Rao M.S., Reddy J.K.
    Proc. Natl. Acad. Sci. U.S.A. 85:9081-9085(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-303.
    Tissue: Liver.
  9. "Identification of an amino acid residue involved in the substrate-binding site of rat liver uricase by site-directed mutagenesis."
    Ito M., Kato S., Nakmura M., Go M., Takagi Y.
    Biochem. Biophys. Res. Commun. 187:101-107(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-164.
  10. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiURIC_RAT
AccessioniPrimary (citable) accession number: P09118
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.