Reviewed, UniProtKB/Swiss-Prot P09118 (URIC_RAT)

Names and origin

Protein names

Recommended name:
    Uricase
    EC=1.7.3.3
Alternative name(s):
    Urate oxidase

Gene names

Name:

Uox

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which spontaneously decomposes to form allantoin.
Catalytic activity	Urate + O2 + H2O = 5-hydroxyisourate + H2O2.
Pathway	Purine metabolism; uric acid degradation; (S)-allantoin from uric acid: step 1/3.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the uricase family.
Sequence caution	The sequence AAA42318.1 differs from that shown. Reason: Frameshift at position 288. The sequence CAA30378.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Purine metabolism
Cellular component	Peroxisome
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base catabolic process Ref.2 Traceable author statement. Source: RGD
Cellular component	peroxisomal matrix Ref.2 Traceable author statement. Source: RGD
Molecular function	urate oxidase activity Ref.2 Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 303	302	Uricase		PRO_0000165990
Regions
	Region	234 – 235	2	Substrate binding By similarity
	Motif	301 – 303	3	Microbody targeting signal Potential
Sites
	Active site	187	1	Charge relay system By similarity
	Active site	235	1	Charge relay system By similarity
	Binding site	68	1	Substrate By similarity
	Binding site	187	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	36	1	N6-acetyllysine By similarity
	Modified residue	39	1	Phosphoserine By similarity
	Modified residue	55	1	N6-acetyllysine By similarity
	Modified residue	118	1	N6-acetyllysine By similarity
	Modified residue	122	1	N6-acetyllysine By similarity
	Modified residue	164	1	N6-acetyllysine By similarity
	Modified residue	185	1	N6-acetyllysine By similarity
	Modified residue	220	1	N6-acetyllysine By similarity
	Modified residue	231	1	Phosphoserine By similarity
	Modified residue	277	1	N6-acetyllysine By similarity
	Modified residue	290	1	N6-acetyllysine By similarity
	Modified residue	301	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	164	1	K → E or I: Loss of activity. Ref.9
	Sequence conflict	55	1	K → D Ref.6
	Sequence conflict	111	1	Y → H in AAA42317. Ref.3
	Sequence conflict	111	1	Y → H Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P09118-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 14CFF2F4E18C7BBB	FASTA	303	34,934
	10 20 30 40 50 60 MAHYHDDYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT LRSKKDYLHG 70 80 90 100 110 120 DNSDIIPTDT IKNTVHVLAK FKGIKSIETF AMNICEHFLS SFSHVTRAHV YVEEVPWKRF 130 140 150 160 170 180 EKNGVKHVHA FIHTPTGTHF CDVEQVRNGP PIIHSGIKDL KVLKTTQSGF EGFIKDQFTT 190 200 210 220 230 240 LPEVKDRCFA TQVYCKWRYQ NRDVDFEATW GAVRDIVLKK FAGPYDRGEY SPSVQKTLYD 250 260 270 280 290 300 IQVLTLSQLP EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVRRKLP SRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of rat uricase chromosomal gene differs greatly from that of the corresponding plant gene." Motojima K., Goto S. FEBS Lett. 264:156-158(1990) [PubMed: 2338140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Cloning and sequence analysis of cDNA for rat liver uricase." Motojima K., Kanaya S., Goto S. J. Biol. Chem. 263:16677-16681(1988) [PubMed: 3182808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase." Alvares K., Nemali M.R., Reddy P.G., Wang X.D., Rao M.S., Reddy J.K. Biochem. Biophys. Res. Commun. 158:991-995(1989) [PubMed: 2920046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[4]	"Nucleotide sequence of cDNA and predicted amino acid sequence of rat liver uricase." Ito M., Suzuki M., Takagi Y. Eur. J. Biochem. 173:459-463(1988) [PubMed: 3267221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[5]	"Rat urate oxidase: cloning and structural analysis of the gene and 5'-flanking region." Wang X.D., Kawano H., Alvares K., Reddy P.G., Getto H., Rao M.S., Reddy J.K. Gene 97:223-229(1991) [PubMed: 1999285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Structural analysis of the gene encoding rat uricase." Ito M., Nakamura M., Ogawa H., Kato S., Takagi Y. Genomics 11:905-913(1991) [PubMed: 1783398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[8]	"Isolation and sequence determination of a cDNA clone for rat peroxisomal urate oxidase: liver-specific expression in the rat." Reddy P.G., Nemali M.R., Reddy M.K., Reddy M.N., Yuan P.M., Yuen S., Laffler T.G., Shiroza T., Kuramitsu H.K., Usuda N., Chisholm R.L., Rao M.S., Reddy J.K. Proc. Natl. Acad. Sci. U.S.A. 85:9081-9085(1988) [PubMed: 3194410] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-303. Tissue: Liver.
[9]	"Identification of an amino acid residue involved in the substrate-binding site of rat liver uricase by site-directed mutagenesis." Ito M., Kato S., Nakmura M., Go M., Takagi Y. Biochem. Biophys. Res. Commun. 187:101-107(1992) [PubMed: 1520291] [Abstract] Cited for: MUTAGENESIS OF LYS-164.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03959 mRNA. Translation: AAA42318.1. Frameshift. X13098 mRNA. Translation: CAA31490.1. M24396 mRNA. Translation: AAA42317.1. X07497 mRNA. Translation: CAA30378.1. Frameshift. M63593 , M63583, M63586, M63587, M63588, M63590, M63591, M63592 Genomic DNA. Translation: AAA61699.1. BC088112 mRNA. Translation: AAH88112.1.
IPI	IPI00231365.
PIR	RDRTU. I54070.
RefSeq	NP_446220.1.
UniGene	Rn.11330
3D structure databases
HSSP	HSSP built from PDB template 1UOX based on UniProtKB Q00511.
ModBase	Search...
PTM databases
PhosphoSite	P09118.
Proteomic databases
PRIDE	P09118.
Genome annotation databases
Ensembl	ENSRNOG00000016339. Rattus norvegicus. [Contig view]
GeneID	114768.
KEGG	rno:114768.
Organism-specific databases
RGD	621369. Uox.
Phylogenomic databases
HOVERGEN	P09118.
OMA	P09118. KTTQSGF.
Enzyme and pathway databases
BRENDA	1.7.3.3. 248.
Gene expression databases
ArrayExpress	P09118.
GermOnline	ENSRNOG00000016339. Rattus norvegicus.
Family and domain databases
InterPro	IPR002042. Uricase. IPR019842. Uricase_CS. [Graphical view]
Gene3D	G3DSA:3.10.270.10. Uricase. 1 hit.
PANTHER	PTHR10395:SF1. Uricase. 1 hit.
Pfam	PF01014. Uricase. 2 hits. [Graphical view]
PRINTS	PR00093. URICASE.
ProDom	PD003367. Uricase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03383. urate_oxi. 1 hit.
PROSITE	PS00366. URICASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	618867.

Entry information

Entry name

URIC_RAT

Accession

Primary (citable) accession number: P09118

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)