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Protein

Tyrocidine synthase 1

Gene

tycA

Organism
Brevibacillus parabrevis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

In the first step of peptide synthesis this enzyme activates phenylalanine and racemizes it to the D-isomer.

Miscellaneous

Tyrocidine is a mixture of four cyclic decapeptides, tyrocidine A (D-Phe-Pro-Phe-D-Phe-Asn-Gln-Tyr-Val-Orn-Leu), B, C, and D, in which Phe, at positions 3, 4, and Tyr residues are gradually replaced by Trp, depending on the relative concentrations of these amino acids in the growth medium.

Catalytic activityi

ATP + L-phenylalanine + H2O = AMP + diphosphate + D-phenylalanine.

Cofactori

pantetheine 4'-phosphateCuratedNote: Binds 1 phosphopantetheine covalently.Curated

Pathwayi: tyrocidine biosynthesis

This protein is involved in the pathway tyrocidine biosynthesis, which is part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the pathway tyrocidine biosynthesis and in Antibiotic biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Ligase
Biological processAntibiotic biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00180.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrocidine synthase 1
Alternative name(s):
Tyrocidine synthase I
Including the following 2 domains:
ATP-dependent D-phenylalanine adenylase
Short name:
D-PheA
Alternative name(s):
D-phenylalanine activase
Phenylalanine racemase [ATP-hydrolyzing] (EC:5.1.1.11)
Gene namesi
Name:tycA
OrganismiBrevibacillus parabrevis
Taxonomic identifieri54914 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001930931 – 1088Tyrocidine synthase 1Add BLAST1088

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei563O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Subunit structurei

Large multienzyme complex of TycA, TycB and TycC.

Structurei

3D structure databases

ProteinModelPortaliP09095.
SMRiP09095.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini528 – 602CarrierPROSITE-ProRule annotationAdd BLAST75

Domaini

One-module-bearing peptide synthase with a C-terminal epimerization domain. Each module incorporates one amino acid into the peptide product and can be further subdivided into domains responsible for substrate adenylation, thiolation, condensation (not for the initiation module), and epimerization (optional), and N methylation (optional).

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR010071. AA_adenyl_domain.
IPR025110. AMP-bd_C.
IPR020459. AMP-binding.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR001242. Condensatn.
IPR010060. NRPS_synth.
IPR009081. PP-bd_ACP.
IPR006162. Ppantetheine_attach_site.
PfamiView protein in Pfam
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
PF00668. Condensation. 1 hit.
PF00550. PP-binding. 1 hit.
PRINTSiPR00154. AMPBINDING.
SUPFAMiSSF47336. SSF47336. 1 hit.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 1 hit.
TIGR01720. NRPS-para261. 1 hit.
PROSITEiView protein in PROSITE
PS00455. AMP_BINDING. 1 hit.
PS50075. CARRIER. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.

Sequencei

Sequence statusi: Complete.

P09095-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLANQANLID NKRELEQHAL VPYAQGKSIH QLFEEQAEAF PDRVAIVFEN
60 70 80 90 100
RRLSYQELNR KANQLARALL EKGVQTDSIV GVMMEKSIEN VIAILAVLKA
110 120 130 140 150
GGAYVPIDIE YPRDRIQYIL QDSQTKIVLT QKSVSQLVHD VGYSGEVVVL
160 170 180 190 200
DEEQLDARET ANLHQPSKPT DLAYVIYTSG TTGKPKGTML EHKGIANLQS
210 220 230 240 250
FFQNSFGVTE QDRIGLFASM SFDASVWEMF MALLSGASLY ILSKQTIHDF
260 270 280 290 300
AAFEHYLSEN ELTIITLPPT YLTHLTPERI TSLRIMITAG SASSAPLVNK
310 320 330 340 350
WKDKLRYINA YGPTETSICA TIWEAPSNQL SVQSVPIGKP IQNTHIYIVN
360 370 380 390 400
EDLQLLPTGS EGELCIGGVG LARGYWNRPD LTAEKFVDNP FVPGEKMYRT
410 420 430 440 450
GDLAKWLTDG TIEFLGRIDH QVKIRGHRIE LGEIESVLLA HEHITEAVVI
460 470 480 490 500
AREDQHAGQY LCAYYISQQE ATPAQLRDYA AQKLPAYMLP SYFVKLDKMP
510 520 530 540 550
LTPNDKIDRK ALPEPDLTAN QSQAAYHPPR TETESILVSI WQNVLGIEKI
560 570 580 590 600
GIRDNFYSLG GDSIQAIQVV ARLHSYQLKL ETKDLLNYPT IEQVALFVKS
610 620 630 640 650
TTRKSDQGII AGNVPLTPIQ KWFFGKNFTN TGHWNQSSVL YRPEGFDPKV
660 670 680 690 700
IQSVMDKIIE HHDALRMVYQ HENGNVVQHN RGLGGQLYDF FSYNLTAQPD
710 720 730 740 750
VQQAIEAETQ RLHSSMNLQE GPLVKVALFQ TLHGDHLFLA IHHLVVDGIS
760 770 780 790 800
WRILFEDLAT GYAQALAGQA ISLPEKTDSF QSWSQWLQEY ANEADLLSEI
810 820 830 840 850
PYWESLESQA KNVSLPKDYE VTDCKQKSVR NMRIRLHPEE TEQLLKHANQ
860 870 880 890 900
AYQTEINDLL LAALGLAFAE WSKLAQIVIH LEGHGREDII EQANVARTVG
910 920 930 940 950
WFTSQYPVLL DLKQTAPLSD YIKLTKENMR KIPRKGIGYD ILKHVTLPEN
960 970 980 990 1000
RGSLSFRVQP EVTFNYLGQF DADMRTELFT RSPYSGGNTL GADGKNNLSP
1010 1020 1030 1040 1050
ESEVYTALNI TGLIEGGELV LTFSYSSEQY REESIQQLSQ SYQKHLLAII
1060 1070 1080
AHCTEKKEVE RTPSDFSVKG LQMEEMDDIF ELLANTLR
Length:1,088
Mass (Da):122,672
Last modified:July 15, 1999 - v2
Checksum:i6AC4804199572027
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197 – 242NLQSF…SLYIL → ICNPFSKIRLASPSKTGSGF LPACRSTHPFGKCSWLCCLA PRVHP in CAA31623 (PubMed:3267240).CuratedAdd BLAST46
Sequence conflicti359 – 360GS → AD in CAA31623 (PubMed:3267240).Curated2
Sequence conflicti496L → I AA sequence (PubMed:8193142).Curated1
Sequence conflicti665L → V in CAA31623 (PubMed:3267240).Curated1
Sequence conflicti737L → F in CAA31623 (PubMed:3267240).Curated1
Sequence conflicti756 – 790EDLAT…WLQEY → KIWQPDTRRHLQGKRSVCPK KRILFKAGHNGCKNN in CAA31623 (PubMed:3267240).CuratedAdd BLAST35
Sequence conflicti876 – 894QIVIH…IEQAN → KSSFIWRGTGARTSSNRQT in CAA31623 (PubMed:3267240).CuratedAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13237 Genomic DNA. Translation: CAA31623.1.
AF004835 Genomic DNA. Translation: AAC45928.1.
M16442 mRNA. Translation: AAA22876.1. Frameshift.
PIRiA26878.
T31074. YGBSTB.

Similar proteinsi

Entry informationi

Entry nameiTYCA_BREPA
AccessioniPrimary (citable) accession number: P09095
Secondary accession number(s): O30407
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 15, 1999
Last modified: May 10, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families