P09093 (CEL3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymotrypsin-like elastase family member 3A
EC=3.4.21.70

Alternative name(s):
Elastase IIIA
Elastase-3A
Protease E

Gene names

Name:	CELA3A
Synonyms:	ELA3, ELA3A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Efficient protease with alanine specificity but only little elastolytic activity.
Catalytic activity	Preferential cleavage: Ala-|-Xaa. Does not hydrolyze elastin.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol metabolic process Traceable author statement Ref.1. Source: ProtInc digestion Traceable author statement Ref.1. Source: ProtInc proteolysis Traceable author statement Ref.1. Source: ProtInc
Molecular_function	serine-type endopeptidase activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Or 16 Potential
	Propeptide	16 – 28	13	Activation peptide Potential		PRO_0000027697
	Chain	29 – 270	242	Chymotrypsin-like elastase family member 3A Ref.1		PRO_0000027698
Regions
	Domain	29 – 268	240	Peptidase S1
Sites
	Active site	73	1	Charge relay system By similarity
	Active site	123	1	Charge relay system By similarity
	Active site	217	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Disulfide bond	58 ↔ 74		By similarity
	Disulfide bond	117 ↔ 120		Probable
	Disulfide bond	157 ↔ 223		By similarity
	Disulfide bond	188 ↔ 204		By similarity
	Disulfide bond	213 ↔ 244		By similarity
Natural variations
	Natural variant	24	1	H → R. Corresponds to variant rs7531336 [ dbSNP | Ensembl ].		VAR_059783
	Natural variant	25	1	S → P. Corresponds to variant rs7533776 [ dbSNP | Ensembl ].		VAR_059784
	Natural variant	31	1	H → N. Corresponds to variant rs7519660 [ dbSNP | Ensembl ].		VAR_059785
	Natural variant	241	1	A → G. Ref.1 Ref.2 Ref.4 Corresponds to variant rs3820285 [ dbSNP | Ensembl ].		VAR_051838
Experimental info
	Sequence conflict	63	1	I → T in AAH05918. Ref.4
	Sequence conflict	106	1	Missing in AAA66350. Ref.1
	Sequence conflict	174	1	K → E in AAH05918. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P09093 [UniParc]. Last modified February 9, 2010. Version 3. Checksum: 576DDB255A4C71EA	FASTA	270	29,489
	10 20 30 40 50 60 MMLRLLSSLL LVAVASGYGP PSSHSSSRVV HGEDAVPYSW PWQVSLQYEK SGSFYHTCGG 70 80 90 100 110 120 SLIAPDWVVT AGHCISRDLT YQVVLGEYNL AVKEGPEQVI PINSEELFVH PLWNRSCVAC 130 140 150 160 170 180 GNDIALIKLS RSAQLGDAVQ LASLPPAGDI LPNKTPCYIT GWGRLYTNGP LPDKLQQARL 190 200 210 220 230 240 PVVDYKHCSR WNWWGSTVKK TMVCAGGYIR SGCNGDSGGP LNCPTEDGGW QVHGVTSFVS 250 260 270 AFGCNFIWKP TVFTRVSAFI DWIEETIASH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning." Tani T., Ohsumi J., Mita K., Takiguchi Y. J. Biol. Chem. 263:1231-1239(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLY-241. Tissue: Pancreas.
[2]	"Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes." Shirasu Y., Takemura K., Yoshida H., Sato Y., Iijima H., Shimada Y., Mikayama T., Ozawa T., Ikeda N., Ishida A., Tamai Y., Matsuki S., Tanaka J., Ikenaga H., Ogawa M. J. Biochem. 104:259-264(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANT GLY-241. Tissue: Pancreas.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-241. Tissue: Pancreas and Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M18700 , M18693, M18694, M18695, M18696, M18697, M18698, M18699 Genomic DNA. Translation: AAA66350.1. D00306 mRNA. Translation: BAA00212.1. AL590556 Genomic DNA. Translation: CAH71875.1. BC005918 mRNA. Translation: AAH05918.1. BC007028 mRNA. Translation: AAH07028.1. BC015103 mRNA. Translation: AAH15103.1.
IPI	IPI00295663.
PIR	A29934.
RefSeq	NP_005738.4. NM_005747.4.
UniGene	Hs.181289. Hs.728752.
3D structure databases
ProteinModelPortal	P09093.
SMR	P09093. Positions 39-270.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000290122.
Protein family/group databases
MEROPS	S01.154.
Polymorphism databases
DMDM	288558842.
Proteomic databases
PaxDb	P09093.
PRIDE	P09093.
Protocols and materials databases
DNASU	10136.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000290122; ENSP00000290122; ENSG00000142789.
GeneID	10136.
KEGG	hsa:10136.
UCSC	uc001bfl.3. human.
Organism-specific databases
CTD	10136.
GeneCards	GC01P022328.
H-InvDB	HIX0000228.
HGNC	HGNC:15944. CELA3A.
HPA	HPA045650.
neXtProt	NX_P09093.
PharmGKB	PA27736.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
InParanoid	P09093.
KO	K01345.
OMA	TASDAND.
OrthoDB	EOG444KM1.
Gene expression databases
ArrayExpress	P09093.
Bgee	P09093.
CleanEx	HS_ELA3A.
Genevestigator	P09093.
GermOnline	ENSG00000142789. Homo sapiens.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL2809.
GenomeRNAi	10136.
NextBio	38345.

Entry information

Entry name

CEL3A_HUMAN

Accession

Primary (citable) accession number: P09093
Secondary accession number(s): B1AQ53, Q9BRW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 9, 2010
Last modified:	April 3, 2013
This is version 130 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.