Reviewed,
UniProtKB/Swiss-Prot P09093 (ELA3A_HUMAN)
Last modified
March 3, 2009.
Version 94.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Elastase-3A EC=3.4.21.70 Alternative name(s): Elastase IIIA Protease E | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 270 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Efficient protease with alanine specificity but only little elastolytic activity. |
| Catalytic activity | Preferential cleavage: Ala-|-Xaa. Does not hydrolyze elastin. |
| Sequence similarities | Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Polymorphism |
| Domain | Signal |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Glycoprotein Zymogen |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cholesterol metabolic process Ref.1 Traceable author statement. Source: ProtInc digestion Ref.1Traceable author statement. Source: ProtInc proteolysis Ref.1Traceable author statement. Source: ProtInc |
| Molecular function | serine-type endopeptidase activity Ref.2 Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 15 | 15 | Or 16 Potential | ||||||||
| Propeptide | 16 – 28 | 13 | Activation peptide Potential | PRO_0000027697 | |||||||
| Chain | 29 – 270 | 242 | Elastase-3A Ref.1 | PRO_0000027698 | |||||||
Regions | |||||||||||
| Domain | 29 – 268 | 240 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 73 | 1 | Charge relay system By similarity | ||||||||
| Active site | 123 | 1 | Charge relay system By similarity | ||||||||
| Active site | 217 | 1 | Charge relay system By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 114 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 58 ↔ 74 | By similarity | |||||||||
| Disulfide bond | 117 ↔ 120 | Probable | |||||||||
| Disulfide bond | 157 ↔ 223 | By similarity | |||||||||
| Disulfide bond | 188 ↔ 204 | By similarity | |||||||||
| Disulfide bond | 213 ↔ 244 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 241 | 1 | G → A: dbSNP rs3820285. Ref.3 | VAR_051838 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 63 | 1 | I → T in AAH05918. Ref.4 | ||||||||
| Sequence conflict | 106 | 1 | Missing in AAA66350. Ref.1 | ||||||||
| Sequence conflict | 174 | 1 | K → E in AAH05918. Ref.4 | ||||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning." Tani T., Ohsumi J., Mita K., Takiguchi Y. J. Biol. Chem. 263:1231-1239(1988) [PubMed: 2826474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Pancreas. |
| [2] | "Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes." Shirasu Y., Takemura K., Yoshida H., Sato Y., Iijima H., Shimada Y., Mikayama T., Ozawa T., Ikeda N., Ishida A., Tamai Y., Matsuki S., Tanaka J., Ikenaga H., Ogawa M. J. Biochem. 104:259-264(1988) [PubMed: 2460440] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Pancreas. |
| [3] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-241. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Prostate. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
M18700 M18699 Genomic DNA. Translation: AAA66350.1. D00306 mRNA. Translation: BAA00212.1. AL590556 Genomic DNA. Translation: CAH71875.1. BC005918 mRNA. Translation: AAH05918.1. BC007028 mRNA. Translation: AAH07028.1. BC015103 mRNA. Translation: AAH15103.1. | |
| IPI | IPI00295663. |
| PIR | A29934. |
| RefSeq | NP_005738.3. |
| UniGene | Hs.654349 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FON based on UniProtKB P05805. |
| SMR | P09093. Positions 23-270. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | S01.154. |
Genome annotation databases | |
| Ensembl | ENSG00000142789. Homo sapiens. [Contig view] |
| GeneID | 10136. |
| KEGG | hsa:10136. |
Organism-specific databases | |
| GeneCards | GC01P022200. |
| H-InvDB | HIX0000228. |
| HGNC | HGNC:15944. ELA3A. |
| PharmGKB | PA27736. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | P09093. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.70. 247. |
Gene expression databases | |
| ArrayExpress | P09093. |
| Bgee | P09093. |
| CleanEx | HS_ELA3A. |
| GermOnline | ENSG00000142789. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view] |
| Pfam | PF00089. Trypsin. 1 hit. [Graphical view] |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 38345. |
Entry information
| Entry name | ELA3A_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P09093 Secondary accession number(s): B1AQ53, Q9BRW4 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


