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P09093 (CEL3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chymotrypsin-like elastase family member 3A

EC=3.4.21.70
Alternative name(s):
Elastase IIIA
Elastase-3A
Protease E
Gene names
Name:CELA3A
Synonyms:ELA3, ELA3A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Efficient protease with alanine specificity but only little elastolytic activity.

Catalytic activity

Preferential cleavage: Ala-|-Xaa. Does not hydrolyze elastin.

Sequence similarities

Belongs to the peptidase S1 family. Elastase subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515Or 16 Potential
Propeptide16 – 2813Activation peptide Potential
PRO_0000027697
Chain29 – 270242Chymotrypsin-like elastase family member 3A Ref.1
PRO_0000027698

Regions

Domain29 – 268240Peptidase S1

Sites

Active site731Charge relay system By similarity
Active site1231Charge relay system By similarity
Active site2171Charge relay system By similarity

Amino acid modifications

Glycosylation1141N-linked (GlcNAc...) Potential
Disulfide bond58 ↔ 74 By similarity
Disulfide bond117 ↔ 120 Probable
Disulfide bond157 ↔ 223 By similarity
Disulfide bond188 ↔ 204 By similarity
Disulfide bond213 ↔ 244 By similarity

Natural variations

Natural variant241H → R.
Corresponds to variant rs7531336 [ dbSNP | Ensembl ].
VAR_059783
Natural variant251S → P.
Corresponds to variant rs7533776 [ dbSNP | Ensembl ].
VAR_059784
Natural variant311H → N.
Corresponds to variant rs7519660 [ dbSNP | Ensembl ].
VAR_059785
Natural variant2411A → G. Ref.1 Ref.2 Ref.4
Corresponds to variant rs3820285 [ dbSNP | Ensembl ].
VAR_051838

Experimental info

Sequence conflict631I → T in AAH05918. Ref.4
Sequence conflict1061Missing in AAA66350. Ref.1
Sequence conflict1741K → E in AAH05918. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P09093 [UniParc].

Last modified February 9, 2010. Version 3.
Checksum: 576DDB255A4C71EA

FASTA27029,489
        10         20         30         40         50         60 
MMLRLLSSLL LVAVASGYGP PSSHSSSRVV HGEDAVPYSW PWQVSLQYEK SGSFYHTCGG 

        70         80         90        100        110        120 
SLIAPDWVVT AGHCISRDLT YQVVLGEYNL AVKEGPEQVI PINSEELFVH PLWNRSCVAC 

       130        140        150        160        170        180 
GNDIALIKLS RSAQLGDAVQ LASLPPAGDI LPNKTPCYIT GWGRLYTNGP LPDKLQQARL 

       190        200        210        220        230        240 
PVVDYKHCSR WNWWGSTVKK TMVCAGGYIR SGCNGDSGGP LNCPTEDGGW QVHGVTSFVS 

       250        260        270 
AFGCNFIWKP TVFTRVSAFI DWIEETIASH 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning."
Tani T., Ohsumi J., Mita K., Takiguchi Y.
J. Biol. Chem. 263:1231-1239(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLY-241.
Tissue: Pancreas.
[2]"Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes."
Shirasu Y., Takemura K., Yoshida H., Sato Y., Iijima H., Shimada Y., Mikayama T., Ozawa T., Ikeda N., Ishida A., Tamai Y., Matsuki S., Tanaka J., Ikenaga H., Ogawa M.
J. Biochem. 104:259-264(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANT GLY-241.
Tissue: Pancreas.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-241.
Tissue: Pancreas and Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M18700 expand/collapse EMBL AC list , M18693, M18694, M18695, M18696, M18697, M18698, M18699 Genomic DNA. Translation: AAA66350.1.
D00306 mRNA. Translation: BAA00212.1.
AL590556 Genomic DNA. Translation: CAH71875.1.
BC005918 mRNA. Translation: AAH05918.1.
BC007028 mRNA. Translation: AAH07028.1.
BC015103 mRNA. Translation: AAH15103.1.
CCDSCCDS220.1.
PIRA29934.
RefSeqNP_005738.4. NM_005747.4.
UniGeneHs.181289.

3D structure databases

ProteinModelPortalP09093.
SMRP09093. Positions 39-270.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP09093. 1 interaction.
STRING9606.ENSP00000290122.

Protein family/group databases

MEROPSS01.154.

Polymorphism databases

DMDM288558842.

Proteomic databases

PaxDbP09093.
PRIDEP09093.

Protocols and materials databases

DNASU10136.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000290122; ENSP00000290122; ENSG00000142789.
GeneID10136.
KEGGhsa:10136.
UCSCuc001bfl.3. human.

Organism-specific databases

CTD10136.
GeneCardsGC01P022328.
H-InvDBHIX0000228.
HGNCHGNC:15944. CELA3A.
HPAHPA045650.
neXtProtNX_P09093.
PharmGKBPA27736.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
InParanoidP09093.
KOK01345.
OMADSILPHE.
PhylomeDBP09093.
TreeFamTF330455.

Gene expression databases

ArrayExpressP09093.
BgeeP09093.
CleanExHS_ELA3A.
GenevestigatorP09093.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCELA3A.
GenomeRNAi10136.
NextBio38345.
PROP09093.

Entry information

Entry nameCEL3A_HUMAN
AccessionPrimary (citable) accession number: P09093
Secondary accession number(s): B1AQ53, Q9BRW4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 9, 2010
Last modified: July 9, 2014
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM