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P09093

- CEL3A_HUMAN

UniProt

P09093 - CEL3A_HUMAN

Protein

Chymotrypsin-like elastase family member 3A

Gene

CELA3A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 3 (09 Feb 2010)
      Previous versions | rss
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    Functioni

    Efficient protease with alanine specificity but only little elastolytic activity.

    Catalytic activityi

    Preferential cleavage: Ala-|-Xaa. Does not hydrolyze elastin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731Charge relay systemBy similarity
    Active sitei123 – 1231Charge relay systemBy similarity
    Active sitei217 – 2171Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: ProtInc

    GO - Biological processi

    1. cholesterol metabolic process Source: ProtInc
    2. digestion Source: ProtInc
    3. proteolysis Source: ProtInc

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chymotrypsin-like elastase family member 3A (EC:3.4.21.70)
    Alternative name(s):
    Elastase IIIA
    Elastase-3A
    Protease E
    Gene namesi
    Name:CELA3A
    Synonyms:ELA3, ELA3A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:15944. CELA3A.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27736.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Or 16Sequence AnalysisAdd
    BLAST
    Propeptidei16 – 2813Activation peptideSequence AnalysisPRO_0000027697Add
    BLAST
    Chaini29 – 270242Chymotrypsin-like elastase family member 3APRO_0000027698Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi58 ↔ 74PROSITE-ProRule annotation
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi117 ↔ 120Curated
    Disulfide bondi157 ↔ 223PROSITE-ProRule annotation
    Disulfide bondi188 ↔ 204PROSITE-ProRule annotation
    Disulfide bondi213 ↔ 244PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP09093.
    PRIDEiP09093.

    Expressioni

    Gene expression databases

    ArrayExpressiP09093.
    BgeeiP09093.
    CleanExiHS_ELA3A.
    GenevestigatoriP09093.

    Organism-specific databases

    HPAiHPA045650.

    Interactioni

    Protein-protein interaction databases

    IntActiP09093. 1 interaction.
    STRINGi9606.ENSP00000290122.

    Structurei

    3D structure databases

    ProteinModelPortaliP09093.
    SMRiP09093. Positions 39-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 268240Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    InParanoidiP09093.
    KOiK01345.
    OMAiDSILPHE.
    PhylomeDBiP09093.
    TreeFamiTF330455.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09093-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMLRLLSSLL LVAVASGYGP PSSHSSSRVV HGEDAVPYSW PWQVSLQYEK    50
    SGSFYHTCGG SLIAPDWVVT AGHCISRDLT YQVVLGEYNL AVKEGPEQVI 100
    PINSEELFVH PLWNRSCVAC GNDIALIKLS RSAQLGDAVQ LASLPPAGDI 150
    LPNKTPCYIT GWGRLYTNGP LPDKLQQARL PVVDYKHCSR WNWWGSTVKK 200
    TMVCAGGYIR SGCNGDSGGP LNCPTEDGGW QVHGVTSFVS AFGCNFIWKP 250
    TVFTRVSAFI DWIEETIASH 270
    Length:270
    Mass (Da):29,489
    Last modified:February 9, 2010 - v3
    Checksum:i576DDB255A4C71EA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti63 – 631I → T in AAH05918. (PubMed:15489334)Curated
    Sequence conflicti106 – 1061Missing in AAA66350. (PubMed:2826474)Curated
    Sequence conflicti174 – 1741K → E in AAH05918. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti24 – 241H → R.
    Corresponds to variant rs7531336 [ dbSNP | Ensembl ].
    VAR_059783
    Natural varianti25 – 251S → P.
    Corresponds to variant rs7533776 [ dbSNP | Ensembl ].
    VAR_059784
    Natural varianti31 – 311H → N.
    Corresponds to variant rs7519660 [ dbSNP | Ensembl ].
    VAR_059785
    Natural varianti241 – 2411A → G.3 Publications
    Corresponds to variant rs3820285 [ dbSNP | Ensembl ].
    VAR_051838

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18700
    , M18693, M18694, M18695, M18696, M18697, M18698, M18699 Genomic DNA. Translation: AAA66350.1.
    D00306 mRNA. Translation: BAA00212.1.
    AL590556 Genomic DNA. Translation: CAH71875.1.
    BC005918 mRNA. Translation: AAH05918.1.
    BC007028 mRNA. Translation: AAH07028.1.
    BC015103 mRNA. Translation: AAH15103.1.
    CCDSiCCDS220.1.
    PIRiA29934.
    RefSeqiNP_005738.4. NM_005747.4.
    UniGeneiHs.181289.

    Genome annotation databases

    EnsembliENST00000290122; ENSP00000290122; ENSG00000142789.
    GeneIDi10136.
    KEGGihsa:10136.
    UCSCiuc001bfl.3. human.

    Polymorphism databases

    DMDMi288558842.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18700
    , M18693 , M18694 , M18695 , M18696 , M18697 , M18698 , M18699 Genomic DNA. Translation: AAA66350.1 .
    D00306 mRNA. Translation: BAA00212.1 .
    AL590556 Genomic DNA. Translation: CAH71875.1 .
    BC005918 mRNA. Translation: AAH05918.1 .
    BC007028 mRNA. Translation: AAH07028.1 .
    BC015103 mRNA. Translation: AAH15103.1 .
    CCDSi CCDS220.1.
    PIRi A29934.
    RefSeqi NP_005738.4. NM_005747.4.
    UniGenei Hs.181289.

    3D structure databases

    ProteinModelPortali P09093.
    SMRi P09093. Positions 39-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P09093. 1 interaction.
    STRINGi 9606.ENSP00000290122.

    Protein family/group databases

    MEROPSi S01.154.

    Polymorphism databases

    DMDMi 288558842.

    Proteomic databases

    PaxDbi P09093.
    PRIDEi P09093.

    Protocols and materials databases

    DNASUi 10136.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000290122 ; ENSP00000290122 ; ENSG00000142789 .
    GeneIDi 10136.
    KEGGi hsa:10136.
    UCSCi uc001bfl.3. human.

    Organism-specific databases

    CTDi 10136.
    GeneCardsi GC01P022328.
    H-InvDB HIX0000228.
    HGNCi HGNC:15944. CELA3A.
    HPAi HPA045650.
    neXtProti NX_P09093.
    PharmGKBi PA27736.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    InParanoidi P09093.
    KOi K01345.
    OMAi DSILPHE.
    PhylomeDBi P09093.
    TreeFami TF330455.

    Miscellaneous databases

    GeneWikii CELA3A.
    GenomeRNAii 10136.
    NextBioi 38345.
    PROi P09093.

    Gene expression databases

    ArrayExpressi P09093.
    Bgeei P09093.
    CleanExi HS_ELA3A.
    Genevestigatori P09093.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning."
      Tani T., Ohsumi J., Mita K., Takiguchi Y.
      J. Biol. Chem. 263:1231-1239(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLY-241.
      Tissue: Pancreas.
    2. "Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes."
      Shirasu Y., Takemura K., Yoshida H., Sato Y., Iijima H., Shimada Y., Mikayama T., Ozawa T., Ikeda N., Ishida A., Tamai Y., Matsuki S., Tanaka J., Ikenaga H., Ogawa M.
      J. Biochem. 104:259-264(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANT GLY-241.
      Tissue: Pancreas.
    3. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-241.
      Tissue: Pancreas and Prostate.

    Entry informationi

    Entry nameiCEL3A_HUMAN
    AccessioniPrimary (citable) accession number: P09093
    Secondary accession number(s): B1AQ53, Q9BRW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: February 9, 2010
    Last modified: October 1, 2014
    This is version 138 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. Peptidase families
      Classification of peptidase families and list of entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3