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Protein

Chymotrypsin-like elastase family member 3A

Gene

CELA3A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Efficient protease with alanine specificity but only little elastolytic activity.

Catalytic activityi

Preferential cleavage: Ala-|-Xaa. Does not hydrolyze elastin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Charge relay systemBy similarity
Active sitei123 – 1231Charge relay systemBy similarity
Active sitei217 – 2171Charge relay systemBy similarity

GO - Molecular functioni

  • serine-type endopeptidase activity Source: ProtInc

GO - Biological processi

  • cholesterol metabolic process Source: ProtInc
  • digestion Source: ProtInc
  • proteolysis Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-like elastase family member 3A (EC:3.4.21.70)
Alternative name(s):
Elastase IIIA
Elastase-3A
Protease E
Gene namesi
Name:CELA3A
Synonyms:ELA3, ELA3A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:15944. CELA3A.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27736.

Polymorphism and mutation databases

BioMutaiCELA3A.
DMDMi288558842.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Or 16Sequence AnalysisAdd
BLAST
Propeptidei16 – 2813Activation peptideSequence AnalysisPRO_0000027697Add
BLAST
Chaini29 – 270242Chymotrypsin-like elastase family member 3APRO_0000027698Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi58 ↔ 74PROSITE-ProRule annotation
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi117 ↔ 120Curated
Disulfide bondi157 ↔ 223PROSITE-ProRule annotation
Disulfide bondi188 ↔ 204PROSITE-ProRule annotation
Disulfide bondi213 ↔ 244PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP09093.
PRIDEiP09093.

Expressioni

Gene expression databases

BgeeiP09093.
CleanExiHS_ELA3A.
ExpressionAtlasiP09093. baseline.
GenevisibleiP09093. HS.

Organism-specific databases

HPAiHPA045650.

Interactioni

Protein-protein interaction databases

IntActiP09093. 1 interaction.
STRINGi9606.ENSP00000290122.

Structurei

3D structure databases

ProteinModelPortaliP09093.
SMRiP09093. Positions 39-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 268240Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
InParanoidiP09093.
KOiK01345.
OMAiIPPNEEP.
PhylomeDBiP09093.
TreeFamiTF330455.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLRLLSSLL LVAVASGYGP PSSHSSSRVV HGEDAVPYSW PWQVSLQYEK
60 70 80 90 100
SGSFYHTCGG SLIAPDWVVT AGHCISRDLT YQVVLGEYNL AVKEGPEQVI
110 120 130 140 150
PINSEELFVH PLWNRSCVAC GNDIALIKLS RSAQLGDAVQ LASLPPAGDI
160 170 180 190 200
LPNKTPCYIT GWGRLYTNGP LPDKLQQARL PVVDYKHCSR WNWWGSTVKK
210 220 230 240 250
TMVCAGGYIR SGCNGDSGGP LNCPTEDGGW QVHGVTSFVS AFGCNFIWKP
260 270
TVFTRVSAFI DWIEETIASH
Length:270
Mass (Da):29,489
Last modified:February 9, 2010 - v3
Checksum:i576DDB255A4C71EA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631I → T in AAH05918 (PubMed:15489334).Curated
Sequence conflicti106 – 1061Missing in AAA66350 (PubMed:2826474).Curated
Sequence conflicti174 – 1741K → E in AAH05918 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti24 – 241H → R.
Corresponds to variant rs7531336 [ dbSNP | Ensembl ].
VAR_059783
Natural varianti25 – 251S → P.
Corresponds to variant rs7533776 [ dbSNP | Ensembl ].
VAR_059784
Natural varianti31 – 311H → N.
Corresponds to variant rs7519660 [ dbSNP | Ensembl ].
VAR_059785
Natural varianti241 – 2411A → G.3 Publications
Corresponds to variant rs3820285 [ dbSNP | Ensembl ].
VAR_051838

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18700
, M18693, M18694, M18695, M18696, M18697, M18698, M18699 Genomic DNA. Translation: AAA66350.1.
D00306 mRNA. Translation: BAA00212.1.
AL590556 Genomic DNA. Translation: CAH71875.1.
BC005918 mRNA. Translation: AAH05918.1.
BC007028 mRNA. Translation: AAH07028.1.
BC015103 mRNA. Translation: AAH15103.1.
CCDSiCCDS220.1.
PIRiA29934.
RefSeqiNP_005738.4. NM_005747.4.
UniGeneiHs.181289.

Genome annotation databases

EnsembliENST00000290122; ENSP00000290122; ENSG00000142789.
GeneIDi10136.
KEGGihsa:10136.
UCSCiuc001bfl.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18700
, M18693, M18694, M18695, M18696, M18697, M18698, M18699 Genomic DNA. Translation: AAA66350.1.
D00306 mRNA. Translation: BAA00212.1.
AL590556 Genomic DNA. Translation: CAH71875.1.
BC005918 mRNA. Translation: AAH05918.1.
BC007028 mRNA. Translation: AAH07028.1.
BC015103 mRNA. Translation: AAH15103.1.
CCDSiCCDS220.1.
PIRiA29934.
RefSeqiNP_005738.4. NM_005747.4.
UniGeneiHs.181289.

3D structure databases

ProteinModelPortaliP09093.
SMRiP09093. Positions 39-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09093. 1 interaction.
STRINGi9606.ENSP00000290122.

Protein family/group databases

MEROPSiS01.154.

Polymorphism and mutation databases

BioMutaiCELA3A.
DMDMi288558842.

Proteomic databases

PaxDbiP09093.
PRIDEiP09093.

Protocols and materials databases

DNASUi10136.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290122; ENSP00000290122; ENSG00000142789.
GeneIDi10136.
KEGGihsa:10136.
UCSCiuc001bfl.3. human.

Organism-specific databases

CTDi10136.
GeneCardsiGC01P022328.
H-InvDBHIX0000228.
HGNCiHGNC:15944. CELA3A.
HPAiHPA045650.
neXtProtiNX_P09093.
PharmGKBiPA27736.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
InParanoidiP09093.
KOiK01345.
OMAiIPPNEEP.
PhylomeDBiP09093.
TreeFamiTF330455.

Miscellaneous databases

GeneWikiiCELA3A.
GenomeRNAii10136.
NextBioi38345.
PROiP09093.

Gene expression databases

BgeeiP09093.
CleanExiHS_ELA3A.
ExpressionAtlasiP09093. baseline.
GenevisibleiP09093. HS.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning."
    Tani T., Ohsumi J., Mita K., Takiguchi Y.
    J. Biol. Chem. 263:1231-1239(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLY-241.
    Tissue: Pancreas.
  2. "Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes."
    Shirasu Y., Takemura K., Yoshida H., Sato Y., Iijima H., Shimada Y., Mikayama T., Ozawa T., Ikeda N., Ishida A., Tamai Y., Matsuki S., Tanaka J., Ikenaga H., Ogawa M.
    J. Biochem. 104:259-264(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANT GLY-241.
    Tissue: Pancreas.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-241.
    Tissue: Pancreas and Prostate.

Entry informationi

Entry nameiCEL3A_HUMAN
AccessioniPrimary (citable) accession number: P09093
Secondary accession number(s): B1AQ53, Q9BRW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 9, 2010
Last modified: July 22, 2015
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.