Reviewed, UniProtKB/Swiss-Prot P09089 (ZEN1_DROME)

Names and origin

Protein names

Recommended name:
    Protein zerknuellt 1
      Short name=ZEN-1

Gene names

Name:	zen
Synonyms:	z1, zen1
ORF Names:	CG1046

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the differentiation of the dorsal-ventral pattern, and does not appear to be involved in the process of segmentation. Ref.1
Subcellular location	Nucleus. Ref.1
Sequence similarities	Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	amnioserosa formation Traceable author statement. Source: FlyBase compound eye development Inferred from mutant phenotype. Source: FlyBase dorsal/ventral axis specification Traceable author statement. Source: FlyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: FlyBase specific transcriptional repressor activity Inferred from mutant phenotype. Source: FlyBase transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Actn	Q9XZS4	1	EBI-122003,EBI-98433
Mad	P42003	1	EBI-122003,EBI-162238

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 353

353

Protein zerknuellt 1

PRO_0000049112

Regions

DNA binding

90 – 149

60

Homeobox

Secondary structure

1

.

.

.

.

353

Helix Strand Turn

Details...

Helix

134 – 142

9

Turn

143 – 146

4

Sequences

	Sequence		Length	Mass (Da)
	P09089-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 1FA64031C160CE2B	FASTA	353	39,302
	10 20 30 40 50 60 MSSVMHYYPV HQAKVGSYSA DPSEVKYSDL IYGHHHDVNP IGLPPNYNQM NSNPTTLNDH 70 80 90 100 110 120 CSPQHVHQQH VSSDENLPSQ PNHDSQRVKL KRSRTAFTSV QLVELENEFK SNMYLYRTRR 130 140 150 160 170 180 IEIAQRLSLC ERQVKIWFQN RRMKFKKDIQ GHREPKSNAK LAQPQAEQSA HRGIVKRLMS 190 200 210 220 230 240 YSQDPREGTA AAEKRPMMAV APVNPKPDYQ ASQKMKTEAS TNNGMCSSAD LSEILEHLAQ 250 260 270 280 290 300 TTAAPQVSTA TSSTGTSTNS ASSSSSGHYS YNVDLVLQSI KQDLEAAAQA WSKSKSAPIL 310 320 330 340 350 ATQSWHPSSQ SQVPTSVHAA PSMNLSWGEP AAKSRKLSVN HMNPCVTSYN YPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the zerknullt region of the Antennapedia gene complex in Drosophila." Rushlow C., Doyle H., Hoey T., Levine M. Genes Dev. 1:1268-1279(1987) [PubMed: 2892759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"Complete sequence of the Antennapedia complex of Drosophila." Celniker S.E., Pfeiffer B., Knafels J., Martin C.H., Mayeda C.A., Palazzolo M.J. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Berkeley.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Larva and Pupae.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X68347 mRNA. Translation: CAA48416.1.
AE001572 Genomic DNA. Translation: AAD19800.1.
AE014297 Genomic DNA. Translation: AAF54087.1.
BT028847 mRNA. Translation: ABI34228.1.

PIR

A43697.

RefSeq

NP_001138018.1.
NP_476793.1.

UniGene

Dm.27227

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KZ2	NMR	-	A	132-147	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:18255N.

IntAct

P09089. 14 interactions.

Proteomic databases

PRIDE

P09089.

Genome annotation databases

Ensembl

FBgn0004053. Drosophila melanogaster. [Contig view]

GeneID

40828.

KEGG

dme:Dmel_CG1046.

NMPDR

fig|7227.3.peg.11451.

Organism-specific databases

FlyBase

FBgn0004053. zen.

Phylogenomic databases

HOGENOM

P09089.

OMA

P09089. YRTRRIE.

Enzyme and pathway databases

BioCyc

DMEL-XXX-02:DMEL-XXX-02-010312-MON.

Gene expression databases

GermOnline

CG1046. Drosophila melanogaster.

Family and domain databases

InterPro

IPR001356. Homeobox.
IPR017970. Homeobox_CS.
IPR012287. Homeodomain-rel.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.

Pfam

PF00046. Homeobox. 1 hit.
[Graphical view]

ProDom

PD000010. Homeobox. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00389. HOX. 1 hit.
[Graphical view]

PROSITE

PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

820800.

Entry information

Entry name

ZEN1_DROME

Accession

Primary (citable) accession number: P09089
Secondary accession number(s): Q0IGS3, Q9VI45

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project