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Protein

Mechanosensory protein 3

Gene

mec-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Specifies differentiation of the set of six touch receptor neurons. Binds cooperatively as a heterodimer with unc-86 to sites in the mec-3 gene promoter.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi217 – 27660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • RNA polymerase II regulatory region sequence-specific DNA binding Source: WormBase
  • transcriptional activator activity, RNA polymerase II transcription factor binding Source: WormBase
  • transcription factor binding Source: WormBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mechanosensory behavior Source: WormBase
  • negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding Source: WormBase
  • neuron differentiation Source: WormBase
  • positive regulation of transcription from RNA polymerase II promoter Source: WormBase
  • response to mechanical stimulus Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mechanosensory protein 3
Gene namesi
Name:mec-3
ORF Names:F01D4.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF01D4.6a; CE27422; WBGene00003167; mec-3.
F01D4.6c; CE42646; WBGene00003167; mec-3.

Subcellular locationi

GO - Cellular componenti

  • RNA polymerase II transcription factor complex Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Mechanosensory protein 3PRO_0000075840Add
BLAST

Proteomic databases

PaxDbiB3GWD3.
P09088.

Expressioni

Gene expression databases

ExpressionAtlasiP09088. baseline.

Interactioni

GO - Molecular functioni

  • transcription factor binding Source: WormBase

Protein-protein interaction databases

IntActiP09088. 78 interactions.
STRINGi6239.F01D4.6a.

Structurei

3D structure databases

ProteinModelPortaliP09088.
SMRiP09088. Positions 28-162, 221-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 8660LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini87 – 15266LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi315 – 3217Asp/Glu-rich (acidic)

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 2 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000064530.
InParanoidiP09088.
KOiK09376.
OMAiCCNEQIY.
OrthoDBiEOG789CG3.
PhylomeDBiP09088.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: P09088-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMLESKPLS AISMVIDSIG VDHENQNKCN CCNEQIYDRY IYRMDNRSYH
60 70 80 90 100
ENCVKCTICE SPLAEKCFWK NGRIYCSQHY YKDHSIHRCA GCKKGVSPTD
110 120 130 140 150
MVYKLKAGLV FHVECHCCSL CGRHLSPGEQ ILVDDTMMTV SCMSHYPPQM
160 170 180 190 200
DDNAPGAIGS AVDIPSCSTE NPIAPYPIDE SFSSAFQVKK EVDAYGYNFE
210 220 230 240 250
HYSFSDFCDD DSRMLKRRGP RTTIKQNQLD VLNEMFSNTP KPSKHARAKL
260 270 280 290 300
ALETGLSMRV IQVWFQNRRS KERRLKHLCN YLRHYEQRGL IPPPIHFRNE
310 320
EMDTTDFNAF CGNFEEEDDE D
Length:321
Mass (Da):37,088
Last modified:February 1, 1994 - v2
Checksum:i9231E7C3F5054853
GO
Isoform cImported (identifier: P09088-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:278
Mass (Da):32,051
Checksum:iACB8AF1608E1BF8E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4343Missing in isoform c. CuratedVSP_058152Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02877 mRNA. Translation: AAA50614.1.
M20244 Genomic DNA. Translation: AAA28108.1.
Z81054 Genomic DNA. Translation: CAB02885.3.
BX284604 Genomic DNA. Translation: CAQ58102.1.
PIRiS28390.
T20458.
RefSeqiNP_001023111.1. NM_001027940.3. [P09088-1]
NP_001129852.1. NM_001136380.2. [P09088-3]
UniGeneiCel.19754.

Genome annotation databases

EnsemblMetazoaiF01D4.6a; F01D4.6a; WBGene00003167. [P09088-1]
F01D4.6c; F01D4.6c; WBGene00003167. [P09088-3]
GeneIDi177938.
KEGGicel:CELE_F01D4.6.
UCSCiF01D4.6a. c. elegans. [P09088-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02877 mRNA. Translation: AAA50614.1.
M20244 Genomic DNA. Translation: AAA28108.1.
Z81054 Genomic DNA. Translation: CAB02885.3.
BX284604 Genomic DNA. Translation: CAQ58102.1.
PIRiS28390.
T20458.
RefSeqiNP_001023111.1. NM_001027940.3. [P09088-1]
NP_001129852.1. NM_001136380.2. [P09088-3]
UniGeneiCel.19754.

3D structure databases

ProteinModelPortaliP09088.
SMRiP09088. Positions 28-162, 221-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09088. 78 interactions.
STRINGi6239.F01D4.6a.

Proteomic databases

PaxDbiB3GWD3.
P09088.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF01D4.6a; F01D4.6a; WBGene00003167. [P09088-1]
F01D4.6c; F01D4.6c; WBGene00003167. [P09088-3]
GeneIDi177938.
KEGGicel:CELE_F01D4.6.
UCSCiF01D4.6a. c. elegans. [P09088-1]

Organism-specific databases

CTDi177938.
WormBaseiF01D4.6a; CE27422; WBGene00003167; mec-3.
F01D4.6c; CE42646; WBGene00003167; mec-3.

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000064530.
InParanoidiP09088.
KOiK09376.
OMAiCCNEQIY.
OrthoDBiEOG789CG3.
PhylomeDBiP09088.

Miscellaneous databases

PROiP09088.

Gene expression databases

ExpressionAtlasiP09088. baseline.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "mec-3, a homeobox-containing gene that specifies differentiation of the touch receptor neurons in C. elegans."
    Way J.C., Chalfie M.
    Cell 54:5-16(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
  2. "Regulation of the mec-3 gene by the C.elegans homeoproteins UNC-86 and MEC-3."
    Xue D., Finney M., Ruvkun G., Chalfie M.
    EMBO J. 11:4969-4979(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION, FUNCTION.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiMEC3_CAEEL
AccessioniPrimary (citable) accession number: P09088
Secondary accession number(s): B3GWD3, O02240, Q7YTQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 1994
Last modified: July 6, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.