P09087 (ABDB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein abdominal-B

Alternative name(s):
Infraabdominal 7
Short name=IAB-7
P3
PH189

Gene names

Name:	Abd-B
ORF Names:	CG10291

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Subcellular location	Nucleus.
Tissue specificity	Isoform M and isoform R are expressed in ectodermal and mesodermal tissues and central nervous system of fourth to ninth embryonic abdominal segments. Later in embryogenesis, expression is seen in visceral mesoderm surrounding hindgut and in two Malpighian tubules. Ref.8
Developmental stage	Highly expressed during embryogenesis, lower expression in larvae, pupae and adults. Ref.8
Sequence similarities	Belongs to the Abd-B homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	determination of genital disc primordium Inferred from mutant phenotype PubMed 15893978. Source: FlyBase external genitalia morphogenesis Inferred from mutant phenotype PubMed 16319117. Source: FlyBase genital disc anterior/posterior pattern formation Inferred from expression pattern PubMed 8798147. Source: FlyBase genital disc sexually dimorphic development Inferred from mutant phenotype PubMed 15893978. Source: FlyBase gonadal mesoderm development Inferred from mutant phenotype PubMed 15454263PubMed 9435287. Source: FlyBase heart development Non-traceable author statement PubMed 12837580. Source: FlyBase imaginal disc-derived female genitalia development Inferred from mutant phenotype PubMed 16971094. Source: FlyBase male genitalia development Inferred from mutant phenotype PubMed 16319117. Source: FlyBase male gonad development Inferred from mutant phenotype PubMed 15454263. Source: FlyBase male pigmentation Inferred from direct assay PubMed 16814723. Source: FlyBase midgut development Traceable author statement PubMed 10512197. Source: FlyBase negative regulation of cardioblast cell fate specification Inferred from mutant phenotype PubMed 12435360. Source: FlyBase negative regulation of salivary gland boundary specification Traceable author statement PubMed 11598957PubMed 12742168. Source: FlyBase negative regulation of striated muscle tissue development Inferred from mutant phenotype PubMed 12397110. Source: FlyBase pole cell migration Traceable author statement PubMed 9637912. Source: FlyBase positive regulation of developmental pigmentation Non-traceable author statement PubMed 12350340. Source: FlyBase positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16814723. Source: FlyBase salivary gland development Non-traceable author statement PubMed 14624839. Source: FlyBase specification of segmental identity, abdomen Inferred from mutant phenotype PubMed 16818450PubMed 16971094. Source: FlyBase spiracle morphogenesis, open tracheal system Inferred from mutant phenotype PubMed 10491268. Source: FlyBase trachea morphogenesis Inferred from mutant phenotype PubMed 21750678. Source: FlyBase
Cellular_component	nucleus Inferred from direct assay PubMed 1972049Ref.3. Source: FlyBase
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding Inferred from direct assay PubMed 9491376. Source: FlyBase sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 16814723. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform M (identifier: P09087-1)

Also known as: Morphogenetic; Abd-BI;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform R (identifier: P09087-2)

Also known as: Regulatory; Abd-BII;

The sequence of this isoform differs from the canonical sequence as follows:
     1-223: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Homeobox protein abdominal-B		PRO_0000200258
Regions
	DNA binding	387 – 446	60	Homeobox
	Compositional bias	1 – 192	192	Gln-rich
	Compositional bias	306 – 311	6	Poly-Ala
	Compositional bias	454 – 458	5	Poly-Asn
Natural variations
	Alternative sequence	1 – 223	223	Missing in isoform R.		VSP_002396
Experimental info
	Sequence conflict	15	1	Q → H in CAB57859. Ref.4
	Sequence conflict	74	1	Missing in CAA34260. Ref.3
	Sequence conflict	177 – 180	4	ASGA → PVR in CAA34260. Ref.3
	Sequence conflict	317 – 318	2	ER → DG in CAA34260. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform M (Morphogenetic) (Abd-BI) [UniParc]. Last modified October 1, 1996. Version 3. Checksum: 55A11B5E97983A5F	FASTA	493	55,096
	10 20 30 40 50 60 MQQHHLQQQQ QQQQQQEQQH LQEQQQHLQQ LHHHAHHHLP QPLHTTSHHH SAHPHLQQQQ 70 80 90 100 110 120 QQQQHAVVAS SPSSVLQQQQ QQSTPTTHST PTHAVMYEDP PPVPLVAVQQ QHLPAPQQQQ 130 140 150 160 170 180 QLQQQQQQQQ QQLATTPVAG ALSPAQTPTG PSAQQQQHLT SPHHQQLPQQ QTPNSVASGA 190 200 210 220 230 240 SSNLQQQQQQ QNAAVAPGQT QIVAPTTASV SPSSVSSQKE DINMSIQLAP LHIPAIRAGP 250 260 270 280 290 300 GFETDTSAAV KRHTAHWAYN DEGFNQHYGS GYYDRKHMFA YPYPETQFPV GQYWGPNYRP 310 320 330 340 350 360 DQTTSAAAAA AYMNEAERHV SAAARQSVEG TSTSSYEPPT YSSPGGLRGY PSENYSSSGA 370 380 390 400 410 420 SGGLSVGAVG PCTPNPGLHE WTGQVSVRKK RKPYSKFQTL ELEKEFLFNA YVSKQKRWEL 430 440 450 460 470 480 ARNLQLTERQ VKIWFQNRRM KNKKNSQRQA NQQNNNNNSS SNHNHAQATQ QHHSGHHLNL 490 SLNMGHHAAK MHQ
	Isoform R (Regulatory) (Abd-BII) [UniParc]. Checksum: 3C951B3B406F1E49	FASTA	270	30,344
	10 20 30 40 50 60 MSIQLAPLHI PAIRAGPGFE TDTSAAVKRH TAHWAYNDEG FNQHYGSGYY DRKHMFAYPY 70 80 90 100 110 120 PETQFPVGQY WGPNYRPDQT TSAAAAAAYM NEAERHVSAA ARQSVEGTST SSYEPPTYSS 130 140 150 160 170 180 PGGLRGYPSE NYSSSGASGG LSVGAVGPCT PNPGLHEWTG QVSVRKKRKP YSKFQTLELE 190 200 210 220 230 240 KEFLFNAYVS KQKRWELARN LQLTERQVKI WFQNRRMKNK KNSQRQANQQ NNNNNSSSNH 250 260 270 NHAQATQQHH SGHHLNLSLN MGHHAAKMHQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of the bithorax complex of Drosophila." Martin C.H., Mayeda C.A., Davis C.A., Ericsson C.L., Knafels J.D., Mathog D.R., Celniker S.E., Lewis E.B., Palazzolo M.J. Proc. Natl. Acad. Sci. U.S.A. 92:8398-8402(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS M AND R). Strain: Canton-S.
[2]	"Evidence that the Abdominal-B r element function is conferred by a trans-regulatory homeoprotein." Delorenzi M., Ali N., Saari G., Henry C., Wilcox M., Bienz M. EMBO J. 7:3223-3231(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM R), CHARACTERIZATION. Tissue: Embryo.
[3]	"The molecular genetics of the bithorax complex of Drosophila: characterization of the products of the Abdominal-B domain." Celniker S.E., Keelan D.J., Lewis E.B. Genes Dev. 3:1424-1436(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM M), CHARACTERIZATION. Tissue: Embryo and Pupae.
[4]	"The morphogenetic and regulatory functions of the Drosophila Abdominal-B gene are encoded in overlapping RNAs transcribed from separate promoters." Zavortink M., Sakonju S. Genes Dev. 3:1969-1981(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM M). Strain: Canton-S. Tissue: Embryo.
[5]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS M AND R). Strain: Berkeley.
[6]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[7]	"Homeo box genes of the Antennapedia and bithorax complexes of Drosophila." Regulski M., Harding K., Kostriken R., Karch F., Levine M., McGinnis W. Cell 43:71-80(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 387-447.
[8]	"Different transcripts of the Drosophila Abd-B gene correlate with distinct genetic sub-functions." Kuziora M.A., McGinnis W. EMBO J. 7:3233-3244(1988) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31961 Genomic DNA. Translation: AAA84402.1. U31961 Genomic DNA. Translation: AAA84403.1. M21173 mRNA. Translation: AAA28400.1. X16134 mRNA. Translation: CAA34260.1. X51663 mRNA. Translation: CAB57859.1. AE014297 Genomic DNA. Translation: AAF55362.1. AE014297 Genomic DNA. Translation: AAF55363.1. X15080 mRNA. Translation: CAA33187.1.
PIR	A32584. A34220. B24780.
RefSeq	NP_524896.2. NM_080157.2. NP_650577.1. NM_142320.1. NP_732180.1. NM_169735.1. NP_732181.1. NM_169736.1. NP_996220.1. NM_206498.1.
UniGene	Dm.1763.
3D structure databases
ProteinModelPortal	P09087.
SMR	P09087. Positions 388-444.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19566N.
IntAct	P09087. 1 interaction.
MINT	MINT-307111.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0083384; FBpp0082826; FBgn0000015.
GeneID	47763.
KEGG	dme:Dmel_CG11648.
Organism-specific databases
CTD	47763.
FlyBase	FBgn0000015. Abd-B.
Phylogenomic databases
eggNOG	NOG286554.
GeneTree	ENSGT00530000063388.
InParanoid	P09087.
KO	K09300.
OMA	THSTPTH.
OrthoDB	EOG43FFDK.
PhylomeDB	P09087.
Gene expression databases
Bgee	P09087.
GermOnline	CG11648. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR020479. Homeobox_metazoa. IPR001356. Homeodomain. IPR009057. Homeodomain-like. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	47763.
NextBio	839106.

Entry information

Entry name

ABDB_DROME

Accession

Primary (citable) accession number: P09087
Secondary accession number(s): Q9VEQ8, Q9VEQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 143 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project