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Protein

POU domain, class 2, transcription factor 2

Gene

POU2F2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi297 – 35660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: MGI
  2. sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  1. cell maturation Source: Ensembl
  2. humoral immune response Source: ProtInc
  3. immunoglobulin secretion involved in immune response Source: Ensembl
  4. mature B cell differentiation Source: Ensembl
  5. positive regulation of transcription, DNA-templated Source: Ensembl
  6. transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 2, transcription factor 2
Alternative name(s):
Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
Octamer-binding protein 2
Short name:
Oct-2
Octamer-binding transcription factor 2
Short name:
OTF-2
Gene namesi
Name:POU2F2
Synonyms:OCT2, OTF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:9213. POU2F2.

Subcellular locationi

Cytoplasm By similarity. Nucleus

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi340 – 3423VIR → FNP: Suppresses DNA-binding ability.

Organism-specific databases

PharmGKBiPA33537.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479POU domain, class 2, transcription factor 2PRO_0000100714Add
BLAST

Proteomic databases

MaxQBiP09086.
PaxDbiP09086.
PRIDEiP09086.

PTM databases

PhosphoSiteiP09086.

Expressioni

Tissue specificityi

Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.2 Publications

Gene expression databases

BgeeiP09086.
CleanExiHS_POU2F2.
ExpressionAtlasiP09086. baseline and differential.
GenevestigatoriP09086.

Organism-specific databases

HPAiCAB002513.

Interactioni

Subunit structurei

Interacts with NR3C1, AR and PGR.1 Publication

Protein-protein interaction databases

BioGridi111448. 15 interactions.
DIPiDIP-51N.
MINTiMINT-194986.
STRINGi9606.ENSP00000373992.

Structurei

Secondary structure

1
479
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi307 – 31812Combined sources
Helixi324 – 3318Combined sources
Turni332 – 3354Combined sources
Helixi338 – 35013Combined sources
Turni356 – 3583Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HDPNMR-A297-359[»]
ProteinModelPortaliP09086.
SMRiP09086. Positions 199-359.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09086.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini195 – 26975POU-specificPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni389 – 41022Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi417 – 4248Gly-rich

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG267922.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000220842.
HOVERGENiHBG052783.
InParanoidiP09086.
KOiK09364.
PhylomeDBiP09086.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P09086-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVHSSMGAPE IRMSKPLEAE KQGLDSPSEH TDTERNGPDT NHQNPQNKTS
60 70 80 90 100
PFSVSPTGPS TKIKAEDPSG DSAPAAPLPP QPAQPHLPQA QLMLTGSQLA
110 120 130 140 150
GDIQQLLQLQ QLVLVPGHHL QPPAQFLLPQ AQQSQPGLLP TPNLFQLPQQ
160 170 180 190 200
TQGALLTSQP RAGLPTQAVT RPTLPDPHLS HPQPPKCLEP PSHPEEPSDL
210 220 230 240 250
EELEQFARTF KQRRIKLGFT QGDVGLAMGK LYGNDFSQTT ISRFEALNLS
260 270 280 290 300
FKNMCKLKPL LEKWLNDAET MSVDSSLPSP NQLSSPSLGF DGLPGRRRKK
310 320 330 340 350
RTSIETNVRF ALEKSFLANQ KPTSEEILLI AEQLHMEKEV IRVWFCNRRQ
360 370 380 390 400
KEKRINPCSA APMLPSPGKP ASYSPHMVTP QGGAGTLPLS QASSSLSTTV
410 420 430 440 450
TTLSSAVGTL HPSRTAGGGG GGGGAAPPLN SIPSVTPPPP ATTNSTNPSP
460 470
QGSHSAIGLS GLNPSTGPGL WWNPAPYQP
Length:479
Mass (Da):51,209
Last modified:January 22, 2007 - v3
Checksum:i8DA661DC07644D43
GO
Isoform 2 (identifier: P09086-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     468-479: Missing.

Show »
Length:467
Mass (Da):49,801
Checksum:i444B5AB65B950050
GO
Isoform 3 (identifier: P09086-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-183: Missing.

Show »
Length:463
Mass (Da):49,461
Checksum:i5DCCA37A76A65AB9
GO
Isoform 4 (identifier: P09086-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-183: Missing.
     400-416: VTTLSSAVGTLHPSRTA → AQTPALKAATRLSACQA
     417-479: Missing.

Show »
Length:400
Mass (Da):43,528
Checksum:i074B384660350FCE
GO
Isoform 5 (identifier: P09086-5) [UniParc]FASTAAdd to basket

Also known as: Oct-2B

The sequence of this isoform differs from the canonical sequence as follows:
     468-479: PGLWWNPAPYQP → STMVGLSSGL...GGPEAGSKAE

Note: Incomplete sequence.

Show »
Length:602
Mass (Da):62,172
Checksum:iF5E4E2B0D206FC15
GO

Sequence cautioni

The sequence CAA32039.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei168 – 18316Missing in isoform 3 and isoform 4. 3 PublicationsVSP_002324Add
BLAST
Alternative sequencei400 – 41617VTTLS…PSRTA → AQTPALKAATRLSACQA in isoform 4. 2 PublicationsVSP_007848Add
BLAST
Alternative sequencei417 – 47963Missing in isoform 4. 2 PublicationsVSP_007849Add
BLAST
Alternative sequencei468 – 47912Missing in isoform 2. 1 PublicationVSP_002325Add
BLAST
Alternative sequencei468 – 47912PGLWW…APYQP → STMVGLSSGLSPALMSNNPL ATIQALASGGTLPLTSLDGS GNLVLGAAGAAPGSPSLVTS PLFLNHTGLPLLSAPPGVGL VSAAAAAVAASISSKSPGLS SSSSSSSSSSSSTCSDVAAQ TPGGPGGPEAGSKAE in isoform 5. 1 PublicationVSP_032187Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36653 mRNA. Translation: AAA36389.1.
X53468 mRNA. Translation: CAA37562.1.
X53469 mRNA. Translation: CAA37565.1.
M36542 mRNA. Translation: AAA36732.1.
X13810 mRNA. Translation: CAA32040.1.
BT007438 mRNA. Translation: AAP36106.1.
BC006101 mRNA. Translation: AAH06101.1.
M36718 mRNA. Translation: AAA59978.1.
X13809 mRNA. Translation: CAA32039.1. Different initiation.
X81030 Genomic DNA. Translation: CAA56933.1.
CCDSiCCDS33035.1. [P09086-3]
CCDS56094.1. [P09086-2]
CCDS56095.1. [P09086-1]
CCDS58665.1. [P09086-4]
PIRiA31753.
A42098.
RefSeqiNP_001193954.1. NM_001207025.2. [P09086-1]
NP_001193955.1. NM_001207026.1. [P09086-2]
NP_001234923.1. NM_001247994.1. [P09086-4]
NP_002689.1. NM_002698.4. [P09086-3]
UniGeneiHs.147381.

Genome annotation databases

EnsembliENST00000389341; ENSP00000373992; ENSG00000028277. [P09086-3]
ENST00000526816; ENSP00000431603; ENSG00000028277. [P09086-1]
ENST00000529067; ENSP00000437224; ENSG00000028277. [P09086-4]
ENST00000529952; ENSP00000436988; ENSG00000028277. [P09086-2]
GeneIDi5452.
KEGGihsa:5452.
UCSCiuc002osn.3. human. [P09086-3]
uc002osp.3. human. [P09086-1]
uc002osq.3. human. [P09086-4]

Polymorphism databases

DMDMi123402.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36653 mRNA. Translation: AAA36389.1.
X53468 mRNA. Translation: CAA37562.1.
X53469 mRNA. Translation: CAA37565.1.
M36542 mRNA. Translation: AAA36732.1.
X13810 mRNA. Translation: CAA32040.1.
BT007438 mRNA. Translation: AAP36106.1.
BC006101 mRNA. Translation: AAH06101.1.
M36718 mRNA. Translation: AAA59978.1.
X13809 mRNA. Translation: CAA32039.1. Different initiation.
X81030 Genomic DNA. Translation: CAA56933.1.
CCDSiCCDS33035.1. [P09086-3]
CCDS56094.1. [P09086-2]
CCDS56095.1. [P09086-1]
CCDS58665.1. [P09086-4]
PIRiA31753.
A42098.
RefSeqiNP_001193954.1. NM_001207025.2. [P09086-1]
NP_001193955.1. NM_001207026.1. [P09086-2]
NP_001234923.1. NM_001247994.1. [P09086-4]
NP_002689.1. NM_002698.4. [P09086-3]
UniGeneiHs.147381.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HDPNMR-A297-359[»]
ProteinModelPortaliP09086.
SMRiP09086. Positions 199-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111448. 15 interactions.
DIPiDIP-51N.
MINTiMINT-194986.
STRINGi9606.ENSP00000373992.

Chemistry

DrugBankiDB08930. Dolutegravir.

PTM databases

PhosphoSiteiP09086.

Polymorphism databases

DMDMi123402.

Proteomic databases

MaxQBiP09086.
PaxDbiP09086.
PRIDEiP09086.

Protocols and materials databases

DNASUi5452.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000389341; ENSP00000373992; ENSG00000028277. [P09086-3]
ENST00000526816; ENSP00000431603; ENSG00000028277. [P09086-1]
ENST00000529067; ENSP00000437224; ENSG00000028277. [P09086-4]
ENST00000529952; ENSP00000436988; ENSG00000028277. [P09086-2]
GeneIDi5452.
KEGGihsa:5452.
UCSCiuc002osn.3. human. [P09086-3]
uc002osp.3. human. [P09086-1]
uc002osq.3. human. [P09086-4]

Organism-specific databases

CTDi5452.
GeneCardsiGC19M042592.
HGNCiHGNC:9213. POU2F2.
HPAiCAB002513.
MIMi164176. gene.
neXtProtiNX_P09086.
PharmGKBiPA33537.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG267922.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000220842.
HOVERGENiHBG052783.
InParanoidiP09086.
KOiK09364.
PhylomeDBiP09086.
TreeFamiTF316413.

Miscellaneous databases

ChiTaRSiPOU2F2. human.
EvolutionaryTraceiP09086.
GenomeRNAii5452.
NextBioi21101.
PROiP09086.
SOURCEiSearch...

Gene expression databases

BgeeiP09086.
CleanExiHS_POU2F2.
ExpressionAtlasiP09086. baseline and differential.
GenevestigatoriP09086.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains."
    Clerc R.G., Corcoran L.M., Lebowitz J.H., Baltimore D., Sharp P.A.
    Genes Dev. 2:1570-1581(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 450-479 (ISOFORM 1).
  2. "A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein."
    Scheidereit C., Cromlish J.A., Gerster T., Kawakami K., Balmaceda C.-G., Currie R.A., Roder R.G.
    Nature 336:551-557(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY.
    Tissue: B-cell lymphoma.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Tissue: Lymph.
  5. "A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells."
    Mueller M.M., Ruppert S., Schaffner W., Matthias P.
    Nature 336:544-551(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-479 (ISOFORM 1).
    Tissue: B-cell.
  6. "Transcription factor Oct-2A contains functionally redundant activating domains and works selectively from a promoter but not from a remote enhancer position in non-lymphoid (HeLa) cells."
    Muller-Immergluck M.M., Schaffner W., Matthias P.
    EMBO J. 9:1625-1634(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 99-161, FUNCTION.
  7. "Short introns interrupting the Oct-2 POU domain may prevent recombination between POU family genes without interfering with potential POU domain 'shuffling' in evolution."
    Matsuo K., Clay O., Kuenzler P., Georgiev O., Urbanek P., Schaffner W.
    Biol. Chem. Hoppe-Seyler 375:675-683(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 168-377.
  8. "A human protein specific for the immunoglobulin octamer DNA motif contains a functional homeobox domain."
    Ko H.-S., Fast P., McBride W., Staudt L.M.
    Cell 55:135-144(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 240-387, FUNCTION.
  9. "Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter."
    Tanaka M., Lai J.-S., Herr W.
    Cell 68:755-767(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 468-479 (ISOFORM 5), FUNCTION.
  10. "Identification of a novel lymphoid specific octamer binding protein (OTF-2B) by proteolytic clipping bandshift assay (PCBA)."
    Schreiber E., Matthias P., Mueller M.M., Schaffner W.
    EMBO J. 7:4221-4229(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION (ISOFORM 5), TISSUE SPECIFICITY.
  11. "Selective binding of steroid hormone receptors to octamer transcription factors determines transcriptional synergism at the mouse mammary tumor virus promoter."
    Prefontaine G.G., Walther R., Giffin W., Lemieux M.E., Pope L., Hache R.J.G.
    J. Biol. Chem. 274:26713-26719(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NR3C1; AR AND PGR.
  12. "Solution structure of a POU-specific homeodomain: 3D-NMR studies of human B-cell transcription factor Oct-2."
    Sivaraja M., Botfield M.C., Mueller M., Jancso A., Weiss M.A.
    Biochemistry 33:9845-9855(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 297-359.

Entry informationi

Entry nameiPO2F2_HUMAN
AccessioniPrimary (citable) accession number: P09086
Secondary accession number(s): Q16648, Q7M4M8, Q9BRS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1988
Last sequence update: January 22, 2007
Last modified: March 3, 2015
This is version 174 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.