P09077 (SCR_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeotic protein Sex combs reduced

Gene names

Name:	Scr
ORF Names:	CG1030

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Controls the segmental transformation of the first to the second thoracic segment (prothorax to mesothorax) and of the labial palps into maxillary palps. In embryo, required for fusion of labial lobes and development of the T1 denticle belt. In adult, expression in the head is necessary for proper development of the labium. In the first thoracic segment of the adult, required for proper development of the sex comb and to suppress improper prothoracic wing development. Ref.9
Subcellular location	Nucleus.
Tissue specificity	In embryo, expressed in the labial and anterior prothoracic segments and the extreme posterior of the maxillary segment. In adult, expressed in the maxillary analgen of the eye and antennal imaginal disk and the labial and T1 leg imaginal disks. Ref.10
Sequence similarities	Belongs to the Antp homeobox family. Deformed subfamily. Contains 1 homeobox DNA-binding domain.
Sequence caution	The sequence AAA19240.1 differs from that shown. Reason: Frameshift at positions 35 and 79. The sequence CAA32637.1 differs from that shown. Reason: Frameshift at positions 93 and 141.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	midgut development Traceable author statement PubMed 10512197. Source: FlyBase positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 20634319. Source: FlyBase salivary gland boundary specification Non-traceable author statement PubMed 14624839. Source: FlyBase sex comb development Inferred from mutant phenotype PubMed 16299391. Source: FlyBase sex differentiation Traceable author statement PubMed 11494318. Source: FlyBase specification of segmental identity, labial segment Traceable author statement PubMed 11090137. Source: FlyBase
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA polymerase II regulatory region sequence-specific DNA binding Inferred from direct assay PubMed 20634319. Source: FlyBase protein homodimerization activity Inferred from direct assay PubMed 22564794. Source: FlyBase sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 417

417

Homeotic protein Sex combs reduced

PRO_0000200264

Regions

DNA binding

324 – 383

60

Homeobox

Motif

303 – 308

6

Antp-type hexapeptide

Compositional bias

33 – 71

39

Gly-rich

Compositional bias

114 – 162

49

Gln-rich

Compositional bias

188 – 200

13

Asn-rich

Amino acid modifications

Modified residue

216

1

Phosphoserine Ref.11

Experimental info

Sequence conflict

118

1

Q → L in AAA19240. Ref.7

Sequence conflict

133 – 135

3

HAH → LAL in AAA19240. Ref.7

Sequence conflict

141

1

A → R in AAA19240. Ref.7

Sequence conflict

258

1

G → R in CAA54348. Ref.3

Sequence conflict

317 – 323

7

TVNANGE → YCESNGQ in CAA28857. Ref.8

Sequence conflict

374

1

N → D in AAA19240. Ref.7

Sequence conflict

379

1

W → L in CAA28857. Ref.8

Sequence conflict

393

1

Y → C in AAA19240. Ref.7

Sequence conflict

402

1

Y → C in AAA19240. Ref.7

Secondary structure

1

.

.

.

.

.

.

.

.

.

417

Helix Strand Turn

Details...

Helix

306 – 308

3

Helix

333 – 343

11

Helix

351 – 360

10

Helix

365 – 380

16

Sequences

	Sequence		Length	Mass (Da)
	P09077 [UniParc]. Last modified June 21, 2005. Version 5. Checksum: 8A3A58A60E0B32BE	FASTA	417	44,263
	10 20 30 40 50 60 MDPDCFAMSS YQFVNSLASC YPQQMNPQQN HPGAGNSSAG GSGGGAGGSG GVVPSGGTNG 70 80 90 100 110 120 GQGSAGAATP GANDYFPAAA AYTPNLYPNT PQAHYANQAA YGGQGNPDMV DYTQLQPQRL 130 140 150 160 170 180 LLQQQQQQQQ QQHAHAAAAV AAQQQQQLAQ QQHPQQQQQQ QQANISCKYA NDPVTPGGSG 190 200 210 220 230 240 GGGVSGSNNN NNSANSNNNN SQSLASPQDL STRDISPKLS PSSVVESVAR SLNKGVLGGS 250 260 270 280 290 300 LAAAAAAAGL NNNHSGSGVS GGPGNVNVPM HSPGGGDSDS ESDSGNEAGS SQNSGNGKKN 310 320 330 340 350 360 PPQIYPWMKR VHLGTSTVNA NGETKRQRTS YTRYQTLELE KEFHFNRYLT RRRRIEIAHA 370 380 390 400 410 LCLTERQIKI WFQNRRMKWK KEHKMASMNI VPYHMGPYGH PYHQFDIHPS QFAHLSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The homeotic gene Sex Combs Reduced of Drosophila: gene structure and embryonic expression." Lemotte P.K., Kuroiwa A., Fessler L.I., Gehring W.J. EMBO J. 8:219-227(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Baumgartner P. Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The sex combs reduced gene of Drosophila melanogaster has multiple transcripts." Andrew D.J. Gene 152:149-155(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete sequence of the Antennapedia complex of Drosophila." Celniker S.E., Pfeiffer B.D., Knafels J., Martin C.H., Mayeda C.A., Palazzolo M.J. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Berkeley.
[5]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[6]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[7]	"Sequence of cDNA of homeodomain protein from sex combs reduced (Scr) gene." Kaufman T.C. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[8]	"Cloning of the homeotic Sex Combs Reduced gene in Drosophila and in situ localization of its transcripts." Kuroiwa A., Kloter U., Baumgartner P., Gehring W.J. EMBO J. 4:3757-3764(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 317-390.
[9]	"A functional and structural analysis of the Sex combs reduced locus of Drosophila melanogaster." Pattatucci A.M., Otteson D.C., Kaufman T.C. Genetics 129:423-441(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Hox genes and the evolution of the arthropod body plan." Hughes C.L., Kaufman T.C. Evol. Dev. 4:459-499(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW ON FUNCTION, TISSUE SPECIFICITY.
[11]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X14475 Genomic DNA. Translation: CAA32637.1. Frameshift.
X77075, X77076 Genomic DNA. Translation: CAA54348.1.
AE001572 Genomic DNA. Translation: AAD19795.1.
AE014297 Genomic DNA. Translation: AAF54082.1.
U10506 mRNA. Translation: AAA19240.1. Frameshift.
X05228 Genomic DNA. Translation: CAA28857.1.

PIR

S03631.

RefSeq

NP_524248.2. NM_079524.4.
NP_996165.1. NM_206443.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2R5Y	X-ray	2.60	A	298-384	[»]
2R5Z	X-ray	2.60	A	298-384	[»]

ProteinModelPortal

P09077.

SMR

P09077. Positions 330-384.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-40017N.

IntAct

P09077. 1 interaction.

STRING

7227.FBpp0081163.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0081657; FBpp0081163; FBgn0003339.
FBtr0081658; FBpp0089393; FBgn0003339.

GeneID

40833.

KEGG

dme:Dmel_CG1030.

Organism-specific databases

CTD

109559.

FlyBase

FBgn0003339. Scr.

Phylogenomic databases

eggNOG

NOG236516.

GeneTree

ENSGT00700000104090.

InParanoid

P09077.

KO

K09311.

OMA

ISCKYAN.

OrthoDB

EOG4H44KH.

PhylomeDB

P09077.

Gene expression databases

Bgee

P09077.

GermOnline

CG1030. Drosophila melanogaster.

Family and domain databases

Gene3D

1.10.10.60. 1 hit.

InterPro

IPR017995. Homeobox_antennapedia.
IPR001827. Homeobox_Antennapedia_CS.
IPR017970. Homeobox_CS.
IPR020479. Homeobox_metazoa.
IPR001356. Homeodomain.
IPR009057. Homeodomain-like.
[Graphical view]

Pfam

PF00046. Homeobox. 1 hit.
[Graphical view]

PRINTS

PR00025. ANTENNAPEDIA.
PR00024. HOMEOBOX.

SMART

SM00389. HOX. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.

PROSITE

PS00032. ANTENNAPEDIA. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

scrib. drosophila.

EvolutionaryTrace

P09077.

GenomeRNAi

40833.

NextBio

820837.

Entry information

Entry name

SCR_DROME

Accession

Primary (citable) accession number: P09077
Secondary accession number(s): Q23990, Q24508, Q9V3Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	June 21, 2005
Last modified:	May 1, 2013
This is version 137 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project