ID   HME2_MOUSE              Reviewed;         324 AA.
AC   P09066;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 2.
DT   24-JAN-2024, entry version 179.
DE   RecName: Full=Homeobox protein engrailed-2;
DE            Short=Homeobox protein en-2;
DE            Short=Mo-En-2;
GN   Name=En2; Synonyms=En-2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=1363401; DOI=10.1002/dvg.1020130505;
RA   Logan C., Hanks M.C., Noble-Topham S., Nallainathan D., Provart N.J.,
RA   Joyner A.L.;
RT   "Cloning and sequence comparison of the mouse, human, and chicken engrailed
RT   genes reveal potential functional domains and regulatory regions.";
RL   Dev. Genet. 13:345-358(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE OF 201-324.
RX   PubMed=2892757; DOI=10.1101/gad.1.1.29;
RA   Joyner A.L., Martin G.R.;
RT   "En-1 and En-2, two mouse genes with sequence homology to the Drosophila
RT   engrailed gene: expression during embryogenesis.";
RL   Genes Dev. 1:29-38(1987).
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Cerebellar granule cells.
CC   -!- DEVELOPMENTAL STAGE: In the adult brain it is found in the cerebellar
CC       granule cell layer while the expression during the gestation period is
CC       region specific, at the junction of the midbrain and hindbrain.
CC   -!- SIMILARITY: Belongs to the engrailed homeobox family. {ECO:0000305}.
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DR   EMBL; L12704; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; L12705; AAA53527.1; -; mRNA.
DR   EMBL; Y00203; CAA68362.1; -; Genomic_DNA.
DR   CCDS; CCDS19143.1; -.
DR   PIR; D48423; D48423.
DR   RefSeq; NP_034264.1; NM_010134.3.
DR   AlphaFoldDB; P09066; -.
DR   BMRB; P09066; -.
DR   SMR; P09066; -.
DR   BioGRID; 199445; 6.
DR   STRING; 10090.ENSMUSP00000036761; -.
DR   GlyGen; P09066; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; P09066; -.
DR   PhosphoSitePlus; P09066; -.
DR   PaxDb; 10090-ENSMUSP00000036761; -.
DR   PeptideAtlas; P09066; -.
DR   ProteomicsDB; 269604; -.
DR   Antibodypedia; 18860; 310 antibodies from 30 providers.
DR   DNASU; 13799; -.
DR   Ensembl; ENSMUST00000036177.9; ENSMUSP00000036761.8; ENSMUSG00000039095.9.
DR   GeneID; 13799; -.
DR   KEGG; mmu:13799; -.
DR   UCSC; uc008wtv.1; mouse.
DR   AGR; MGI:95390; -.
DR   CTD; 2020; -.
DR   MGI; MGI:95390; En2.
DR   VEuPathDB; HostDB:ENSMUSG00000039095; -.
DR   eggNOG; KOG0493; Eukaryota.
DR   GeneTree; ENSGT00940000159935; -.
DR   HOGENOM; CLU_051739_2_0_1; -.
DR   InParanoid; P09066; -.
DR   OMA; GESGDQC; -.
DR   OrthoDB; 2897502at2759; -.
DR   PhylomeDB; P09066; -.
DR   TreeFam; TF106461; -.
DR   BioGRID-ORCS; 13799; 1 hit in 80 CRISPR screens.
DR   ChiTaRS; En2; mouse.
DR   PRO; PR:P09066; -.
DR   Proteomes; UP000000589; Chromosome 5.
DR   RNAct; P09066; Protein.
DR   Bgee; ENSMUSG00000039095; Expressed in presumptive midbrain and 68 other cell types or tissues.
DR   ExpressionAtlas; P09066; baseline and differential.
DR   GO; GO:0001650; C:fibrillar center; ISO:MGI.
DR   GO; GO:0005730; C:nucleolus; ISO:MGI.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0071542; P:dopaminergic neuron differentiation; IGI:MGI.
DR   GO; GO:1990403; P:embryonic brain development; IGI:ParkinsonsUK-UCL.
DR   GO; GO:0030902; P:hindbrain development; IGI:MGI.
DR   GO; GO:0030901; P:midbrain development; IGI:MGI.
DR   GO; GO:0043524; P:negative regulation of neuron apoptotic process; IGI:ParkinsonsUK-UCL.
DR   GO; GO:0048666; P:neuron development; IGI:MGI.
DR   GO; GO:0030182; P:neuron differentiation; IGI:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR019549; Homeobox-engrailed_C-terminal.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR000747; Homeobox_engrailed.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR019737; Homoebox-engrailed_CS.
DR   PANTHER; PTHR24341; HOMEOBOX PROTEIN ENGRAILED; 1.
DR   PANTHER; PTHR24341:SF5; HOMEOBOX PROTEIN ENGRAILED-2; 1.
DR   Pfam; PF10525; Engrail_1_C_sig; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00026; ENGRAILED.
DR   PRINTS; PR00024; HOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00033; ENGRAILED; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   Genevisible; P09066; MM.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..324
FT                   /note="Homeobox protein engrailed-2"
FT                   /id="PRO_0000196068"
FT   DNA_BIND        235..294
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          89..174
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          215..240
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        12..38
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   324 AA;  33817 MW;  D1D9D602A1225A53 CRC64;
     MEEKDSKPSE TAAEAQRQPE PSSGGGSGGG SSPSDSDTGR RRALMLPEVL QAPGNHQHPH
     RITNFFIDNI LRPEFGRRKD AGTCCAGAGG ARGGEGGAGT TEGGGGGAGG AEQLLGARES
     RPNPACAPSA GGTLSAAAGD PAVDGEGGSK TLSLHGGAKK PGDPGGSLDG VLKARGLGGG
     DLSVSSDSDS SQASATLGAQ PMLWPAWVYC TRYSDRPSSG PRSRKPKKKN PNKEDKRPRT
     AFTAEQLQRL KAEFQTNRYL TEQRRQSLAQ ELSLNESQIK IWFQNKRAKI KKATGNKNTL
     AVHLMAQGLY NHSTTAKEGK SDSE
//