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Protein

Homeobox protein engrailed-2

Gene

En2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi235 – 29460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. hindbrain development Source: MGI
  2. midbrain development Source: MGI
  3. neuron development Source: MGI
  4. neuron differentiation Source: MGI
  5. positive regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein engrailed-2
Short name:
Homeobox protein en-2
Short name:
Mo-En-2
Gene namesi
Name:En2
Synonyms:En-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:95390. En2.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: Ensembl
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Homeobox protein engrailed-2PRO_0000196068Add
BLAST

Proteomic databases

PaxDbiP09066.
PRIDEiP09066.

PTM databases

PhosphoSiteiP09066.

Expressioni

Tissue specificityi

Cerebellar granule cells.

Developmental stagei

In the adult brain it is found in the cerebellar granule cell layer while the expression during the gestation period is region specific, at the junction of the midbrain and hindbrain.

Gene expression databases

BgeeiP09066.
CleanExiMM_EN2.
ExpressionAtlasiP09066. baseline and differential.
GenevestigatoriP09066.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036761.

Structurei

3D structure databases

ProteinModelPortaliP09066.
SMRiP09066. Positions 214-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi24 – 307Poly-Gly
Compositional biasi103 – 1108Poly-Gly

Sequence similaritiesi

Belongs to the engrailed homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG306728.
HOGENOMiHOG000247054.
HOVERGENiHBG005975.
InParanoidiP09066.
KOiK09319.
OMAiRESHNSP.
OrthoDBiEOG7VTDN8.
PhylomeDBiP09066.
TreeFamiTF106461.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR019549. Homeobox-engrailed_C-terminal.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000747. Homeodomain_engrailed.
IPR019737. Homoebox-engrailed_CS.
[Graphical view]
PfamiPF10525. Engrail_1_C_sig. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00026. ENGRAILED.
PR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00033. ENGRAILED. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09066-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEKDSKPSE TAAEAQRQPE PSSGGGSGGG SSPSDSDTGR RRALMLPEVL
60 70 80 90 100
QAPGNHQHPH RITNFFIDNI LRPEFGRRKD AGTCCAGAGG ARGGEGGAGT
110 120 130 140 150
TEGGGGGAGG AEQLLGARES RPNPACAPSA GGTLSAAAGD PAVDGEGGSK
160 170 180 190 200
TLSLHGGAKK PGDPGGSLDG VLKARGLGGG DLSVSSDSDS SQASATLGAQ
210 220 230 240 250
PMLWPAWVYC TRYSDRPSSG PRSRKPKKKN PNKEDKRPRT AFTAEQLQRL
260 270 280 290 300
KAEFQTNRYL TEQRRQSLAQ ELSLNESQIK IWFQNKRAKI KKATGNKNTL
310 320
AVHLMAQGLY NHSTTAKEGK SDSE
Length:324
Mass (Da):33,817
Last modified:February 1, 1994 - v2
Checksum:iD1D9D602A1225A53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12704 Genomic DNA. No translation available.
L12705 mRNA. Translation: AAA53527.1.
Y00203 Genomic DNA. Translation: CAA68362.1.
CCDSiCCDS19143.1.
PIRiD48423.
RefSeqiNP_034264.1. NM_010134.3.
UniGeneiMm.4298.

Genome annotation databases

EnsembliENSMUST00000036177; ENSMUSP00000036761; ENSMUSG00000039095.
GeneIDi13799.
KEGGimmu:13799.
UCSCiuc008wtv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12704 Genomic DNA. No translation available.
L12705 mRNA. Translation: AAA53527.1.
Y00203 Genomic DNA. Translation: CAA68362.1.
CCDSiCCDS19143.1.
PIRiD48423.
RefSeqiNP_034264.1. NM_010134.3.
UniGeneiMm.4298.

3D structure databases

ProteinModelPortaliP09066.
SMRiP09066. Positions 214-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036761.

PTM databases

PhosphoSiteiP09066.

Proteomic databases

PaxDbiP09066.
PRIDEiP09066.

Protocols and materials databases

DNASUi13799.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036177; ENSMUSP00000036761; ENSMUSG00000039095.
GeneIDi13799.
KEGGimmu:13799.
UCSCiuc008wtv.1. mouse.

Organism-specific databases

CTDi2020.
MGIiMGI:95390. En2.

Phylogenomic databases

eggNOGiNOG306728.
HOGENOMiHOG000247054.
HOVERGENiHBG005975.
InParanoidiP09066.
KOiK09319.
OMAiRESHNSP.
OrthoDBiEOG7VTDN8.
PhylomeDBiP09066.
TreeFamiTF106461.

Miscellaneous databases

NextBioi284554.
PROiP09066.
SOURCEiSearch...

Gene expression databases

BgeeiP09066.
CleanExiMM_EN2.
ExpressionAtlasiP09066. baseline and differential.
GenevestigatoriP09066.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR019549. Homeobox-engrailed_C-terminal.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000747. Homeodomain_engrailed.
IPR019737. Homoebox-engrailed_CS.
[Graphical view]
PfamiPF10525. Engrail_1_C_sig. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00026. ENGRAILED.
PR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00033. ENGRAILED. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence comparison of the mouse, human, and chicken engrailed genes reveal potential functional domains and regulatory regions."
    Logan C., Hanks M.C., Noble-Topham S., Nallainathan D., Provart N.J., Joyner A.L.
    Dev. Genet. 13:345-358(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis."
    Joyner A.L., Martin G.R.
    Genes Dev. 1:29-38(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 201-324.

Entry informationi

Entry nameiHME2_MOUSE
AccessioniPrimary (citable) accession number: P09066
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 1994
Last modified: January 7, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.