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Protein

Homeobox protein engrailed-1

Gene

En1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi312 – 37160HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • adult locomotory behavior Source: ParkinsonsUK-UCL
  • cerebellum development Source: ParkinsonsUK-UCL
  • dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
  • dorsal/ventral pattern formation Source: MGI
  • drinking behavior Source: ParkinsonsUK-UCL
  • embryonic brain development Source: ParkinsonsUK-UCL
  • embryonic forelimb morphogenesis Source: MGI
  • embryonic limb morphogenesis Source: ParkinsonsUK-UCL
  • hindbrain development Source: MGI
  • limb development Source: MGI
  • midbrain development Source: ParkinsonsUK-UCL
  • midbrain-hindbrain boundary development Source: MGI
  • motor learning Source: ParkinsonsUK-UCL
  • multicellular organism growth Source: MGI
  • negative regulation of neuron apoptotic process Source: ParkinsonsUK-UCL
  • negative regulation of neuron death Source: ParkinsonsUK-UCL
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neuron development Source: MGI
  • neuron differentiation Source: MGI
  • pigmentation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • proximal/distal pattern formation Source: MGI
  • regulation of gene expression Source: MGI
  • social behavior Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein engrailed-1
Short name:
Homeobox protein en-1
Short name:
Mo-En-1
Gene namesi
Name:En1
Synonyms:En-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:95389. En1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Homeobox protein engrailed-1PRO_0000196063Add
BLAST

Proteomic databases

MaxQBiP09065.
PaxDbiP09065.
PRIDEiP09065.

PTM databases

iPTMnetiP09065.
PhosphoSiteiP09065.

Expressioni

Gene expression databases

BgeeiP09065.
CleanExiMM_EN1.
GenevisibleiP09065. MM.

Interactioni

Protein-protein interaction databases

BioGridi199444. 2 interactions.
STRINGi10090.ENSMUSP00000078659.

Structurei

3D structure databases

ProteinModelPortaliP09065.
SMRiP09065. Positions 312-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi52 – 8736Pro-richAdd
BLAST
Compositional biasi73 – 8715Poly-ProAdd
BLAST
Compositional biasi207 – 22822Poly-AlaAdd
BLAST

Sequence similaritiesi

Belongs to the engrailed homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0493. Eukaryota.
ENOG4111P06. LUCA.
GeneTreeiENSGT00840000129733.
HOGENOMiHOG000247054.
HOVERGENiHBG005975.
InParanoidiP09065.
KOiK09319.
OMAiNCGPPDG.
OrthoDBiEOG7VTDN8.
TreeFamiTF106461.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR019549. Homeobox-engrailed_C-terminal.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000747. Homeodomain_engrailed.
IPR019737. Homoebox-engrailed_CS.
[Graphical view]
PfamiPF10525. Engrail_1_C_sig. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00026. ENGRAILED.
PR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00033. ENGRAILED. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEQQPEPKS QRDSGLGAVA AAAPSGLSLS LSPGASGSSG SDGDSVPVSP
60 70 80 90 100
QPAPPSPPAA PCLPPLAHHP HLPPHPPPPP PPPPPPPQHL AAPAHQPQPA
110 120 130 140 150
AQLHRTTNFF IDNILRPDFG CKKEQPLPQL LVASAAAGGG AAAGGGSRVE
160 170 180 190 200
RDRGQTGAGR DPVHSLGTRA SGAASLLCAP DANCGPPDGS QPATAVSAGA
210 220 230 240 250
SKAGNPAAAA AAAAAAAAAA VAAAAAAASK PSDSGGGSGG NAGSPGAQGA
260 270 280 290 300
KFPEHNPAIL LMGSANGGPV VKTDSQQPLV WPAWVYCTRY SDRPSSGPRT
310 320 330 340 350
RKLKKKKNEK EDKRPRTAFT AEQLQRLKAE FQANRYITEQ RRQTLAQELS
360 370 380 390 400
LNESQIKIWF QNKRAKIKKA TGIKNGLALH LMAQGLYNHS TTTVQDKDES

E
Length:401
Mass (Da):40,981
Last modified:July 27, 2011 - v3
Checksum:i05456FD3815AA02A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971S → G in AAA03660 (PubMed:1363401).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12703 mRNA. Translation: AAA03660.2.
AK140408 mRNA. Translation: BAE24375.1.
Y00201 Genomic DNA. Translation: CAA68361.1.
CCDSiCCDS15235.1.
PIRiA48423.
RefSeqiNP_034263.2. NM_010133.2.
UniGeneiMm.2657.

Genome annotation databases

EnsembliENSMUST00000079721; ENSMUSP00000078659; ENSMUSG00000058665.
GeneIDi13798.
KEGGimmu:13798.
UCSCiuc007cjm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12703 mRNA. Translation: AAA03660.2.
AK140408 mRNA. Translation: BAE24375.1.
Y00201 Genomic DNA. Translation: CAA68361.1.
CCDSiCCDS15235.1.
PIRiA48423.
RefSeqiNP_034263.2. NM_010133.2.
UniGeneiMm.2657.

3D structure databases

ProteinModelPortaliP09065.
SMRiP09065. Positions 312-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199444. 2 interactions.
STRINGi10090.ENSMUSP00000078659.

PTM databases

iPTMnetiP09065.
PhosphoSiteiP09065.

Proteomic databases

MaxQBiP09065.
PaxDbiP09065.
PRIDEiP09065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079721; ENSMUSP00000078659; ENSMUSG00000058665.
GeneIDi13798.
KEGGimmu:13798.
UCSCiuc007cjm.2. mouse.

Organism-specific databases

CTDi2019.
MGIiMGI:95389. En1.

Phylogenomic databases

eggNOGiKOG0493. Eukaryota.
ENOG4111P06. LUCA.
GeneTreeiENSGT00840000129733.
HOGENOMiHOG000247054.
HOVERGENiHBG005975.
InParanoidiP09065.
KOiK09319.
OMAiNCGPPDG.
OrthoDBiEOG7VTDN8.
TreeFamiTF106461.

Miscellaneous databases

PROiP09065.
SOURCEiSearch...

Gene expression databases

BgeeiP09065.
CleanExiMM_EN1.
GenevisibleiP09065. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR019549. Homeobox-engrailed_C-terminal.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000747. Homeodomain_engrailed.
IPR019737. Homoebox-engrailed_CS.
[Graphical view]
PfamiPF10525. Engrail_1_C_sig. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00026. ENGRAILED.
PR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00033. ENGRAILED. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence comparison of the mouse, human, and chicken engrailed genes reveal potential functional domains and regulatory regions."
    Logan C., Hanks M.C., Noble-Topham S., Nallainathan D., Provart N.J., Joyner A.L.
    Dev. Genet. 13:345-358(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Medulla oblongata.
  3. "En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis."
    Joyner A.L., Martin G.R.
    Genes Dev. 1:29-38(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 278-401.
  4. "Expression during embryogenesis of a mouse gene with sequence homology to the Drosophila engrailed gene."
    Joyner A.L., Kornberg T., Coleman K.G., Cox D.R., Martin G.R.
    Cell 43:29-37(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 298-401.
  5. "Conservation of engrailed-like homeobox sequences during vertebrate evolution."
    Holland P.W.H., Williams N.A.
    FEBS Lett. 277:250-252(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 321-380.

Entry informationi

Entry nameiHME1_MOUSE
AccessioniPrimary (citable) accession number: P09065
Secondary accession number(s): Q3USF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.