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Protein

2-oxoisovalerate dehydrogenase subunit beta

Gene

bkdA2

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.

Cofactori

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-6667-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoisovalerate dehydrogenase subunit beta (EC:1.2.4.4)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
Short name:
BCKDH E1-beta
Gene namesi
Name:bkdA2
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3393392-oxoisovalerate dehydrogenase subunit betaPRO_0000162251Add
BLAST

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

DIPiDIP-6209N.
IntActiP09061. 1 interaction.
MINTiMINT-100336.
STRINGi160488.PP_4402.

Structurei

Secondary structure

1
339
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Helixi8 – 2215Combined sources
Beta strandi26 – 305Combined sources
Beta strandi34 – 363Combined sources
Turni42 – 454Combined sources
Helixi46 – 505Combined sources
Turni52 – 543Combined sources
Beta strandi55 – 573Combined sources
Helixi62 – 7514Combined sources
Beta strandi78 – 825Combined sources
Helixi86 – 883Combined sources
Helixi90 – 923Combined sources
Helixi93 – 975Combined sources
Turni98 – 1025Combined sources
Helixi103 – 1064Combined sources
Turni107 – 1093Combined sources
Beta strandi116 – 1216Combined sources
Beta strandi124 – 1263Combined sources
Beta strandi129 – 1324Combined sources
Helixi136 – 1394Combined sources
Beta strandi146 – 1483Combined sources
Helixi153 – 16513Combined sources
Beta strandi166 – 1683Combined sources
Beta strandi170 – 1756Combined sources
Helixi176 – 1783Combined sources
Beta strandi179 – 1813Combined sources
Beta strandi187 – 1893Combined sources
Helixi194 – 1963Combined sources
Beta strandi201 – 2055Combined sources
Beta strandi216 – 2183Combined sources
Beta strandi224 – 2274Combined sources
Helixi231 – 24212Combined sources
Beta strandi247 – 2504Combined sources
Beta strandi252 – 2565Combined sources
Helixi259 – 26911Combined sources
Beta strandi272 – 2787Combined sources
Helixi285 – 29511Combined sources
Beta strandi296 – 2994Combined sources
Beta strandi305 – 3084Combined sources
Helixi319 – 3224Combined sources
Helixi326 – 33510Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QS0X-ray2.40B2-339[»]
2BP7X-ray2.90B/D/F/H1-339[»]
ProteinModelPortaliP09061.
SMRiP09061. Positions 2-339.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09061.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105CPP. Bacteria.
COG0022. LUCA.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

P09061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTTMTMIQ ALRSAMDVML ERDDNVVVYG QDVGYFGGVF RCTEGLQTKY
60 70 80 90 100
GKSRVFDAPI SESGIVGTAV GMGAYGLRPV VEIQFADYFY PASDQIVSEM
110 120 130 140 150
ARLRYRSAGE FIAPLTLRMP CGGGIYGGQT HSQSPEAMFT QVCGLRTVMP
160 170 180 190 200
SNPYDAKGLL IASIECDDPV IFLEPKRLYN GPFDGHHDRP VTPWSKHPHS
210 220 230 240 250
AVPDGYYTVP LDKAAITRPG NDVSVLTYGT TVYVAQVAAE ESGVDAEVID
260 270 280 290 300
LRSLWPLDLD TIVESVKKTG RCVVVHEATR TCGFGAELVS LVQEHCFHHL
310 320 330
EAPIERVTGW DTPYPHAQEW AYFPGPSRVG AALKKVMEV
Length:339
Mass (Da):37,134
Last modified:November 1, 1988 - v1
Checksum:i318FE1E403B2AEA5
GO

Sequence cautioni

The sequence AAA65615 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57613 Genomic DNA. Translation: AAA65616.1.
M57613 Genomic DNA. Translation: AAA65615.1. Different initiation.
PIRiS01318. DEPSEB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57613 Genomic DNA. Translation: AAA65616.1.
M57613 Genomic DNA. Translation: AAA65615.1. Different initiation.
PIRiS01318. DEPSEB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QS0X-ray2.40B2-339[»]
2BP7X-ray2.90B/D/F/H1-339[»]
ProteinModelPortaliP09061.
SMRiP09061. Positions 2-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6209N.
IntActiP09061. 1 interaction.
MINTiMINT-100336.
STRINGi160488.PP_4402.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CPP. Bacteria.
COG0022. LUCA.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-6667-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP09061.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiODBB_PSEPU
AccessioniPrimary (citable) accession number: P09061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 11, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.