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Protein

2-oxoisovalerate dehydrogenase subunit beta

Gene

bkdA2

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.

Cofactori

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Thiamine pyrophosphate

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoisovalerate dehydrogenase subunit beta (EC:1.2.4.4)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
Short name:
BCKDH E1-beta
Gene namesi
Name:bkdA2
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001622511 – 3392-oxoisovalerate dehydrogenase subunit betaAdd BLAST339

Proteomic databases

PRIDEiP09061.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

DIPiDIP-6209N.
IntActiP09061. 1 interactor.
MINTiMINT-100336.
STRINGi160488.PP_4402.

Structurei

Secondary structure

1339
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Helixi8 – 22Combined sources15
Beta strandi26 – 30Combined sources5
Beta strandi34 – 36Combined sources3
Turni42 – 45Combined sources4
Helixi46 – 50Combined sources5
Turni52 – 54Combined sources3
Beta strandi55 – 57Combined sources3
Helixi62 – 75Combined sources14
Beta strandi78 – 82Combined sources5
Helixi86 – 88Combined sources3
Helixi90 – 92Combined sources3
Helixi93 – 97Combined sources5
Turni98 – 102Combined sources5
Helixi103 – 106Combined sources4
Turni107 – 109Combined sources3
Beta strandi116 – 121Combined sources6
Beta strandi124 – 126Combined sources3
Beta strandi129 – 132Combined sources4
Helixi136 – 139Combined sources4
Beta strandi146 – 148Combined sources3
Helixi153 – 165Combined sources13
Beta strandi166 – 168Combined sources3
Beta strandi170 – 175Combined sources6
Helixi176 – 178Combined sources3
Beta strandi179 – 181Combined sources3
Beta strandi187 – 189Combined sources3
Helixi194 – 196Combined sources3
Beta strandi201 – 205Combined sources5
Beta strandi216 – 218Combined sources3
Beta strandi224 – 227Combined sources4
Helixi231 – 242Combined sources12
Beta strandi247 – 250Combined sources4
Beta strandi252 – 256Combined sources5
Helixi259 – 269Combined sources11
Beta strandi272 – 278Combined sources7
Helixi285 – 295Combined sources11
Beta strandi296 – 299Combined sources4
Beta strandi305 – 308Combined sources4
Helixi319 – 322Combined sources4
Helixi326 – 335Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QS0X-ray2.40B2-339[»]
2BP7X-ray2.90B/D/F/H1-339[»]
ProteinModelPortaliP09061.
SMRiP09061.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09061.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105CPP. Bacteria.
COG0022. LUCA.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

P09061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTTMTMIQ ALRSAMDVML ERDDNVVVYG QDVGYFGGVF RCTEGLQTKY
60 70 80 90 100
GKSRVFDAPI SESGIVGTAV GMGAYGLRPV VEIQFADYFY PASDQIVSEM
110 120 130 140 150
ARLRYRSAGE FIAPLTLRMP CGGGIYGGQT HSQSPEAMFT QVCGLRTVMP
160 170 180 190 200
SNPYDAKGLL IASIECDDPV IFLEPKRLYN GPFDGHHDRP VTPWSKHPHS
210 220 230 240 250
AVPDGYYTVP LDKAAITRPG NDVSVLTYGT TVYVAQVAAE ESGVDAEVID
260 270 280 290 300
LRSLWPLDLD TIVESVKKTG RCVVVHEATR TCGFGAELVS LVQEHCFHHL
310 320 330
EAPIERVTGW DTPYPHAQEW AYFPGPSRVG AALKKVMEV
Length:339
Mass (Da):37,134
Last modified:November 1, 1988 - v1
Checksum:i318FE1E403B2AEA5
GO

Sequence cautioni

The sequence AAA65615 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57613 Genomic DNA. Translation: AAA65616.1.
M57613 Genomic DNA. Translation: AAA65615.1. Different initiation.
PIRiS01318. DEPSEB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57613 Genomic DNA. Translation: AAA65616.1.
M57613 Genomic DNA. Translation: AAA65615.1. Different initiation.
PIRiS01318. DEPSEB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QS0X-ray2.40B2-339[»]
2BP7X-ray2.90B/D/F/H1-339[»]
ProteinModelPortaliP09061.
SMRiP09061.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6209N.
IntActiP09061. 1 interactor.
MINTiMINT-100336.
STRINGi160488.PP_4402.

Proteomic databases

PRIDEiP09061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CPP. Bacteria.
COG0022. LUCA.

Miscellaneous databases

EvolutionaryTraceiP09061.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiODBB_PSEPU
AccessioniPrimary (citable) accession number: P09061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.