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Protein

2-oxoisovalerate dehydrogenase subunit alpha

Gene

bkdA1

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.

Cofactori

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Thiamine pyrophosphate

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoisovalerate dehydrogenase subunit alpha (EC:1.2.4.4)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
Short name:
BCKDH E1-alpha
Gene namesi
Name:bkdA1
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001622491 – 4102-oxoisovalerate dehydrogenase subunit alphaAdd BLAST410

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

DIPiDIP-6208N.
IntActiP09060. 1 interactor.
MINTiMINT-100326.
STRINGi160488.PP_4401.

Structurei

Secondary structure

1410
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 26Combined sources3
Helixi44 – 47Combined sources4
Helixi48 – 51Combined sources4
Helixi67 – 69Combined sources3
Helixi74 – 99Combined sources26
Beta strandi102 – 104Combined sources3
Turni110 – 112Combined sources3
Helixi113 – 122Combined sources10
Beta strandi127 – 130Combined sources4
Helixi136 – 141Combined sources6
Helixi146 – 154Combined sources9
Turni160 – 163Combined sources4
Helixi173 – 175Combined sources3
Beta strandi182 – 185Combined sources4
Helixi186 – 200Combined sources15
Beta strandi207 – 212Combined sources6
Helixi214 – 217Combined sources4
Helixi219 – 231Combined sources13
Beta strandi235 – 241Combined sources7
Beta strandi243 – 245Combined sources3
Helixi250 – 253Combined sources4
Turni254 – 257Combined sources4
Helixi262 – 266Combined sources5
Beta strandi270 – 275Combined sources6
Helixi279 – 294Combined sources16
Beta strandi300 – 305Combined sources6
Helixi318 – 320Combined sources3
Helixi326 – 329Combined sources4
Helixi335 – 345Combined sources11
Helixi351 – 373Combined sources23
Beta strandi378 – 380Combined sources3
Helixi388 – 390Combined sources3
Beta strandi391 – 396Combined sources6
Helixi399 – 406Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QS0X-ray2.40A2-408[»]
2BP7X-ray2.90A/C/E/G1-410[»]
ProteinModelPortaliP09060.
SMRiP09060.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09060.

Family & Domainsi

Sequence similaritiesi

Belongs to the BCKDHA family.Curated

Phylogenomic databases

eggNOGiENOG4107R0V. Bacteria.
COG1071. LUCA.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR022593. Oxoisoval_DH_suAlpha_N_dom.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
PF12573. OxoDH_E1alpha_N. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.

Sequencei

Sequence statusi: Complete.

P09060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEYAPLRLH VPEPTGRPGC QTDFSYLRLN DAGQARKPPV DVDAADTADL
60 70 80 90 100
SYSLVRVLDE QGDAQGPWAE DIDPQILRQG MRAMLKTRIF DSRMVVAQRQ
110 120 130 140 150
KKMSFYMQSL GEEAIGSGQA LALNRTDMCF PTYRQQSILM ARDVSLVEMI
160 170 180 190 200
CQLLSNERDP LKGRQLPIMY SVREAGFFTI SGNLATQFVQ AVGWAMASAI
210 220 230 240 250
KGDTKIASAW IGDGATAESD FHTALTFAHV YRAPVILNVV NNQWAISTFQ
260 270 280 290 300
AIAGGESTTF AGRGVGCGIA SLRVDGNDFV AVYAASRWAA ERARRGLGPS
310 320 330 340 350
LIEWVTYRAG PHSTSDDPSK YRPADDWSHF PLGDPIARLK QHLIKIGHWS
360 370 380 390 400
EEEHQATTAE FEAAVIAAQK EAEQYGTLAN GHIPSAASMF EDVYKEMPDH
410
LRRQRQELGV
Length:410
Mass (Da):45,268
Last modified:February 1, 1996 - v2
Checksum:i0C998460CCFB9CF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57613 Genomic DNA. Translation: AAA65614.1.
PIRiS01317. DEPSXA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57613 Genomic DNA. Translation: AAA65614.1.
PIRiS01317. DEPSXA.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QS0X-ray2.40A2-408[»]
2BP7X-ray2.90A/C/E/G1-410[»]
ProteinModelPortaliP09060.
SMRiP09060.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6208N.
IntActiP09060. 1 interactor.
MINTiMINT-100326.
STRINGi160488.PP_4401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107R0V. Bacteria.
COG1071. LUCA.

Miscellaneous databases

EvolutionaryTraceiP09060.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR022593. Oxoisoval_DH_suAlpha_N_dom.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
PF12573. OxoDH_E1alpha_N. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiODBA_PSEPU
AccessioniPrimary (citable) accession number: P09060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.