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Protein

Leukemia inhibitory factor

Gene

Lif

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

GO - Molecular functioni

  • cytokine activity Source: MGI
  • growth factor activity Source: MGI
  • leukemia inhibitory factor receptor binding Source: MGI
  • signaling receptor binding Source: MGI

GO - Biological processi

  • blood vessel remodeling Source: MGI
  • decidualization Source: MGI
  • embryo implantation Source: MGI
  • immune response Source: InterPro
  • leukemia inhibitory factor signaling pathway Source: MGI
  • lung alveolus development Source: MGI
  • lung development Source: MGI
  • lung lobe morphogenesis Source: MGI
  • lung vasculature development Source: MGI
  • maternal process involved in female pregnancy Source: MGI
  • muscle organ morphogenesis Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of ERK1 and ERK2 cascade Source: MGI
  • negative regulation of hormone secretion Source: MGI
  • negative regulation of meiotic nuclear division Source: MGI
  • neuron development Source: MGI
  • positive regulation of astrocyte differentiation Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of histone H3-K27 acetylation Source: MGI
  • positive regulation of macrophage differentiation Source: MGI
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis Source: MGI
  • positive regulation of peptidyl-serine phosphorylation Source: MGI
  • positive regulation of peptidyl-serine phosphorylation of STAT protein Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of protein localization to nucleus Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of tyrosine phosphorylation of STAT protein Source: MGI
  • regulation of cell differentiation Source: MGI
  • regulation of metanephric nephron tubule epithelial cell differentiation Source: MGI
  • regulation of RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  • spongiotrophoblast differentiation Source: MGI
  • stem cell differentiation Source: MGI
  • stem cell population maintenance Source: MGI
  • trophoblast giant cell differentiation Source: MGI
  • tyrosine phosphorylation of STAT protein Source: MGI

Keywordsi

Molecular functionCytokine, Growth factor

Enzyme and pathway databases

ReactomeiR-MMU-6788467 IL-6-type cytokine receptor ligand interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Leukemia inhibitory factor
Short name:
LIF
Alternative name(s):
Differentiation-stimulating factor
Short name:
D factor
Gene namesi
Name:Lif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96787 Lif

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000001771624 – 203Leukemia inhibitory factorAdd BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32N-linked (GlcNAc...) asparagine1
Disulfide bondi35 ↔ 1571 Publication
Disulfide bondi41 ↔ 1541 Publication
Glycosylationi57N-linked (GlcNAc...) asparagine1
Disulfide bondi83 ↔ 1861 Publication
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi96N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi119N-linked (GlcNAc...) asparagine1
Glycosylationi139N-linked (GlcNAc...) asparagine1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP09056
PaxDbiP09056
PRIDEiP09056

Expressioni

Gene expression databases

BgeeiENSMUSG00000034394
CleanExiMM_LIF
ExpressionAtlasiP09056 baseline and differential
GenevisibleiP09056 MM

Interactioni

GO - Molecular functioni

  • cytokine activity Source: MGI
  • growth factor activity Source: MGI
  • leukemia inhibitory factor receptor binding Source: MGI
  • signaling receptor binding Source: MGI

Protein-protein interaction databases

DIPiDIP-5771N
STRINGi10090.ENSMUSP00000067066

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 34Combined sources3
Helixi35 – 37Combined sources3
Helixi45 – 71Combined sources27
Beta strandi73 – 75Combined sources3
Helixi76 – 78Combined sources3
Helixi79 – 82Combined sources4
Helixi99 – 127Combined sources29
Helixi132 – 158Combined sources27
Helixi178 – 200Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A7MNMR-A24-203[»]
1LKIX-ray2.00A24-203[»]
ProteinModelPortaliP09056
SMRiP09056
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09056

Family & Domainsi

Sequence similaritiesi

Belongs to the LIF/OSM family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410ITBQ Eukaryota
ENOG410YVPP LUCA
GeneTreeiENSGT00390000000059
HOGENOMiHOG000059647
HOVERGENiHBG006267
InParanoidiP09056
KOiK05419
OMAiKYHVAHV
OrthoDBiEOG091G0HWD
PhylomeDBiP09056
TreeFamiTF336245

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR003624 Leukemia_IF
IPR001581 Leukemia_IF/oncostatin
IPR019827 Leukemia_IF/oncostatin_CS
PANTHERiPTHR10633 PTHR10633, 1 hit
PfamiView protein in Pfam
PF01291 LIF_OSM, 1 hit
PRINTSiPR01883 LEUKAEMIAIF
SMARTiView protein in SMART
SM00080 LIF_OSM, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit
PROSITEiView protein in PROSITE
PS00590 LIF_OSM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLAAGIVP LLLLVLHWKH GAGSPLPITP VNATCAIRHP CHGNLMNQIK
60 70 80 90 100
NQLAQLNGSA NALFISYYTA QGEPFPNNVE KLCAPNMTDF PSFHGNGTEK
110 120 130 140 150
TKLVELYRMV AYLSASLTNI TRDQKVLNPT AVSLQVKLNA TIDVMRGLLS
160 170 180 190 200
NVLCRLCNKY RVGHVDVPPV PDHSDKEAFQ RKKLGCQLLG TYKQVISVVV

QAF
Length:203
Mass (Da):22,287
Last modified:November 1, 1988 - v1
Checksum:iF5C65EF11A67A835
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12810 mRNA Translation: CAA31299.1
X06381 mRNA Translation: CAA29680.1
AF065917 mRNA Translation: AAC17912.1
AF065918 mRNA Translation: AAC17913.1
M63419 Genomic DNA Translation: AAA37211.1
M57691 mRNA Translation: AAA63388.1
CCDSiCCDS24383.1
PIRiA36282
RefSeqiNP_001034626.1, NM_001039537.2
NP_032527.1, NM_008501.2
XP_006514605.1, XM_006514542.3
XP_006514606.1, XM_006514543.3
UniGeneiMm.4964

Genome annotation databases

EnsembliENSMUST00000066283; ENSMUSP00000067066; ENSMUSG00000034394
GeneIDi16878
KEGGimmu:16878
UCSCiuc007hut.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLIF_MOUSE
AccessioniPrimary (citable) accession number: P09056
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health