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Reviewed, UniProtKB/Swiss-Prot P09055 (ITB1_MOUSE)

Last modified November 24, 2009. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Integrin beta-1
Alternative name(s):
    Fibronectin receptor subunit beta
    Integrin VLA-4 subunit beta
    CD_antigen=CD29
Gene names
Name: Itgb1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length798 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Ref.7 Ref.8 Ref.10

Subunit structure

Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha-5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5. Interacts with FLNA, FLNB, FLNC and RANBP9. Interacts with KRT1 in the presence of GNB2L1 and SRC By similarity. Interacts with RAB21 By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Melanosome By similarity.

Post-translational modification

The cysteine residues are involved in intrachain disulfide bonds By similarity.

Sequence similarities

Belongs to the integrin beta chain family.

Contains 1 VWFA domain.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell membrane
Membrane
   DomainRepeat
Signal
Transmembrane
   Molecular functionIntegrin
Receptor
   PTMAcetylation
Disulfide bond
Glycoprotein
Phosphoprotein
Gene Ontology (GO)
   Biological processG1/S transition of mitotic cell cycle

Inferred from mutant phenotype. Source: MGI

cardiac muscle cell differentiation

Inferred from mutant phenotype. Source: MGI

cell fate specification

Inferred from mutant phenotype. Source: MGI

cell-matrix adhesion

Inferred from electronic annotation. Source: InterPro

germ cell migration

Inferred from mutant phenotype. Source: MGI

in utero embryonic development

Inferred from mutant phenotype. Source: MGI

integrin-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of cell differentiation

Inferred from mutant phenotype. Source: MGI

positive regulation of cell proliferation

Inferred from mutant phenotype. Source: MGI

regulation of cell cycle

Inferred from mutant phenotype. Source: MGI

sarcomere organization

Inferred from mutant phenotype. Source: MGI

   Cellular componentintegrin complex

Inferred from electronic annotation. Source: InterPro

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

neuromuscular junction

Inferred from direct assay. Source: MGI

sarcolemma

Inferred from direct assay. Source: MGI

synaptosome

Inferred from direct assay. Source: MGI

   Molecular functionintegrin binding

Inferred from physical interaction. Source: MGI

receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 798778Integrin beta-1
PRO_0000016335

Regions

Topological domain21 – 728708Extracellular Potential
Transmembrane729 – 75123 Potential
Topological domain752 – 79847Cytoplasmic Potential
Domain140 – 378239VWFA
Repeat466 – 51550I
Repeat516 – 55944II
Repeat560 – 59839III
Repeat599 – 63537IV
Region466 – 635170Cysteine-rich tandem repeats

Amino acid modifications

Modified residue1861Phosphoserine Ref.11
Modified residue7771Phosphothreonine Ref.11
Modified residue7831Phosphotyrosine Ref.9
Modified residue7941N6-acetyllysine By similarity
Modified residue7951Phosphotyrosine By similarity
Glycosylation501N-linked (GlcNAc...) Potential
Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation2121N-linked (GlcNAc...) Potential
Glycosylation2691N-linked (GlcNAc...) Potential
Glycosylation3631N-linked (GlcNAc...) Ref.12
Glycosylation3661N-linked (GlcNAc...) Ref.12
Glycosylation3761N-linked (GlcNAc...) Ref.12
Glycosylation4061N-linked (GlcNAc...) Potential
Glycosylation4171N-linked (GlcNAc...) Potential
Glycosylation4811N-linked (GlcNAc...) Potential
Glycosylation5201N-linked (GlcNAc...) Potential
Glycosylation5841N-linked (GlcNAc...) Potential
Glycosylation6691N-linked (GlcNAc...) Ref.12
Disulfide bond27 ↔ 464 By similarity
Disulfide bond35 ↔ 45 By similarity
Disulfide bond38 ↔ 75 By similarity
Disulfide bond48 ↔ 64 By similarity
Disulfide bond207 ↔ 213 By similarity
Disulfide bond261 ↔ 301 By similarity
Disulfide bond401 ↔ 415 By similarity
Disulfide bond435 ↔ 691 By similarity
Disulfide bond462 ↔ 466 By similarity
Disulfide bond477 ↔ 489 By similarity
Disulfide bond486 ↔ 525 By similarity
Disulfide bond491 ↔ 500 By similarity
Disulfide bond502 ↔ 516 By similarity
Disulfide bond531 ↔ 536 By similarity
Disulfide bond533 ↔ 568 By similarity
Disulfide bond538 ↔ 553 By similarity
Disulfide bond555 ↔ 560 By similarity
Disulfide bond574 ↔ 579 By similarity
Disulfide bond576 ↔ 607 By similarity
Disulfide bond581 ↔ 590 By similarity
Disulfide bond592 ↔ 599 By similarity
Disulfide bond613 ↔ 618 By similarity
Disulfide bond615 ↔ 661 By similarity
Disulfide bond620 ↔ 630 By similarity
Disulfide bond633 ↔ 636 By similarity
Disulfide bond640 ↔ 649 By similarity
Disulfide bond646 ↔ 723 By similarity
Disulfide bond665 ↔ 699 By similarity

Experimental info

Mutagenesis7831Y → F: Reduced endocytosis; when associated with F-795. Ref.10
Mutagenesis7951Y → F: Reduced endocytosis; when associated with F-783. Ref.10
Sequence conflict51L → Q in BAC40532. Ref.2
Sequence conflict721K → E in BAE35290. Ref.2
Sequence conflict3851E → D in BAC36379. Ref.2
Sequence conflict3851E → P in CAA33272. Ref.5
Sequence conflict3921G → A in CAA33272. Ref.5
Sequence conflict4071G → E in BAC36379. Ref.2
Sequence conflict443 – 4453IKI → HSKL in CAA33272. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P09055-1 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 26788F7F0A168B56

FASTA79888,231
        10         20         30         40         50         60 
MNLQLVSWIG LISLICSVFG QTDKNRCLKA NAKSCGECIQ AGPNCGWCTN TTFLQEGMPT 

        70         80         90        100        110        120 
SARCDDLEAL KKKGCQPSDI ENPRGSQTIK KNKNVTNRSK GMAEKLRPED ITQIQPQQLL 

       130        140        150        160        170        180 
LKLRSGEPQK FTLKFKRAED YPIDLYYLMD LSYSMKDDLE NVKSLGTDLM NEMRRITSDF 

       190        200        210        220        230        240 
RIGFGSFVEK TVMPYISTTP AKLRNPCTSE QNCTSPFSYK NVLSLTDRGE FFNELVGQQR 

       250        260        270        280        290        300 
ISGNLDSPEG GFDAIMQVAV CGSLIGWRNV TRLLVFSTDA GFHFAGDGKL GGIVLPNDGQ 

       310        320        330        340        350        360 
CHLENNVYTM SHYYDYPSIA HLVQKLSENN IQTIFAVTEE FQPVYKELKN LIPKSAVGTL 

       370        380        390        400        410        420 
SGNSSNVIQL IIDAYNSLSS EVILENSKLP DGVTINYKSY CKNGVNGTGE NGRKCSNISI 

       430        440        450        460        470        480 
GDEVQFEISI TANKCPNKES ETIKIKPLGF TEEVEVVLQF ICKCNCQSHG IPASPKCHEG 

       490        500        510        520        530        540 
NGTFECGACR CNEGRVGRHC ECSTDEVNSE DMDAYCRKEN SSEICSNNGE CVCGQCVCRK 

       550        560        570        580        590        600 
RDNTNEIYSG KFCECDNFNC DRSNGLICGG NGVCRCRVCE CYPNYTGSAC DCSLDTGPCL 

       610        620        630        640        650        660 
ASNGQICNGR GICECGACKC TDPKFQGPTC ETCQTCLGVC AEHKECVQCR AFNKGEKKDT 

       670        680        690        700        710        720 
CAQECSHFNL TKVESREKLP QPVQVDPVTH CKEKDIDDCW FYFTYSVNGN NEAIVHVVET 

       730        740        750        760        770        780 
PDCPTGPDII PIVAGVVAGI VLIGLALLLI WKLLMIIHDR REFAKFEKEK MNAKWDTGEN 

       790 
PIYKSAVTTV VNPKYEGK 

« Hide

References

« Hide 'large scale' references
[1]"Murine mRNA for the beta-subunit of integrin is increased in BALB/c-3T3 cells entering the G1 phase from the G0 state."
Tominaga S.
FEBS Lett. 238:315-319(1988) [PubMed: 3262537] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Head, Osteoclast, Placenta and Thymocyte.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[5]"Molecular cloning of a murine fibronectin receptor and its expression during inflammation. Expression of VLA-5 is increased in activated peritoneal macrophages in a manner discordant from major histocompatibility complex class II."
Holers V.M., Ruff T.G., Parks D.L., McDonald J.A., Ballard L.L., Brown E.J.
J. Exp. Med. 169:1589-1605(1989) [PubMed: 2523953] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-798.
Strain: BALB/c.
[6]"Coordinate induction of fibronectin, fibronectin receptor, tropomyosin, and actin genes in serum-stimulated fibroblasts."
Ryseck R.P., MacDonald-Bravo H., Zerial M., Bravo R.
Exp. Cell Res. 180:537-545(1989) [PubMed: 2521606] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 595-798.
[7]"The muscle integrin binding protein (MIBP) interacts with alpha7beta1 integrin and regulates cell adhesion and laminin matrix deposition."
Li J., Rao H., Burkin D., Kaufman S.J., Wu C.
Dev. Biol. 261:209-219(2003) [PubMed: 12941630] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ITGB1BP3.
[8]"NG2 proteoglycan promotes endothelial cell motility and angiogenesis via engagement of galectin-3 and alpha3beta1 integrin."
Fukushi J., Makagiansar I.T., Stallcup W.B.
Mol. Biol. Cell 15:3580-3590(2004) [PubMed: 15181153] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ITGA3; LGALS3 AND CSPG4.
[9]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-783, MASS SPECTROMETRY.
Tissue: Mast cell.
[10]"Integrin trafficking regulated by Rab21 is necessary for cytokinesis."
Pellinen T., Tuomi S., Arjonen A., Wolf M., Edgren H., Meyer H., Grosse R., Kitzing T., Rantala J.K., Kallioniemi O., Faessler R., Kallio M., Ivaska J.
Dev. Cell 15:371-385(2008) [PubMed: 18804435] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF TYR-783 AND TYR-795.
[11]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186 AND THR-777, MASS SPECTROMETRY.
Tissue: Macrophage.
[12]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: Identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 0:0-0(2009) [PubMed: 19656770] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-363; ASN-366; ASN-376 AND ASN-669, MASS SPECTROMETRY.
Tissue: Myoblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

Y00769 mRNA. Translation: CAA68738.1.
AK076526 mRNA. Translation: BAC36379.1. Different initiation.
AK088729 mRNA. Translation: BAC40532.1.
AK159689 mRNA. Translation: BAE35290.1.
AK167682 mRNA. Translation: BAE39731.1.
CH466525 Genomic DNA. Translation: EDL11832.1.
BC050906 mRNA. Translation: AAH50906.1.
X15202 mRNA. Translation: CAA33272.1.
IPIIPI00132474.
PIRIJMSFB. PL0104.
S01659.
RefSeqNP_034708.1.
UniGeneMm.263396

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP09055. 6 interactions.
STRINGP09055.

PTM databases

PhosphoSiteP09055.

Proteomic databases

PRIDEP09055.

Genome annotation databases

EnsemblENSMUST00000090006; ENSMUSP00000087457; ENSMUSG00000025809; Mus musculus. [Genome view]
GeneID16412.
KEGGmmu:16412.
UCSCuc009nzv.1. mouse.

Organism-specific databases

CTD16412.
MGIMGI:96610. Itgb1.

Phylogenomic databases

HOGENOMP09055.
HOVERGENP09055.
OMAVDPLSHC
OrthoDBEOG9M3CVK

Gene expression databases

ArrayExpressP09055.
BgeeP09055.
CleanExMM_ITGB1.
GenevestigatorP09055.
GermOnlineENSMUSG00000025809. Mus musculus.

Family and domain databases

InterProIPR013111. EGF_extracell.
IPR015812. Integrin_bsu.
IPR015438. Integrin_bsu-1_C.
IPR001169. Integrin_bsu_C.
IPR014836. Integrin_bsu_cyt.
IPR002369. Integrin_bsu_N.
IPR012012. Integrin_bsu_subgr.
IPR012896. Integrin_bsu_tail.
IPR003659. Plexin-like.
IPR002035. VWF_A.
[Graphical view]
Gene3DG3DSA:1.20.5.630. Integrin_bsu_cyt. 1 hit.
PANTHERPTHR10082. Integrin_beta_C. 1 hit.
PTHR10082:SF14. Integrin_bsu-1_C. 1 hit.
PfamPF07974. EGF_2. 3 hits.
PF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFPIRSF002512. Integrin_B. 1 hit.
PRINTSPR01186. INTEGRINB.
SMARTSM00187. INB. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 2 hits. Uncertain.
PS00243. INTEGRIN_BETA. 3 hits.
PS50234. VWFA. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio289597.
SOURCESearch...

Entry information

Entry nameITB1_MOUSE
AccessionPrimary (citable) accession number: P09055
Secondary accession number(s): Q3TIW5 expand/collapse secondary AC list , Q3TWH6, Q8BTU0, Q8BVU1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 24, 2009
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents