Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P09053 (AVTA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Valine--pyruvate aminotransferase

EC=2.6.1.66
Alternative name(s):
Alanine--valine transaminase
Transaminase C
Gene names
Name:avtA
Ordered Locus Names:b3572, JW5652
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biosynthesis of alanine. Ref.6 Ref.8

Catalytic activity

L-valine + pyruvate = 3-methyl-2-oxobutanoate + L-alanine.

Cofactor

Pyridoxal phosphate.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Induction

Modestly repressed by alanine and leucine via Lrp. Amino acid limitation causes repression by promoting the accumulation of L-alanine or L-leucine or both. AvtA is also repressed by L-alpha-aminobutyric acid and other nonprotein amino acids which are structurally similar to L-alanine. Ref.7 Ref.8

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Sequence caution

The sequence CAA68546.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

The sequence CAA68546.1 differs from that shown. Reason:

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417Valine--pyruvate aminotransferase
PRO_0000123941

Amino acid modifications

Modified residue2491N6-(pyridoxal phosphate)lysine By similarity

Experimental info

Sequence conflict2081A → G in AAB18549. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P09053 [UniParc].

Last modified October 11, 2004. Version 3.
Checksum: 55A0CA53F0E697A2

FASTA41746,711
        10         20         30         40         50         60 
MTFSLFGDKF TRHSGITLLM EDLNDGLRTP GAIMLGGGNP AQIPEMQDYF QTLLTDMLES 

        70         80         90        100        110        120 
GKATDALCNY DGPQGKTELL TLLAGMLREK LGWDIEPQNI ALTNGSQSAF FYLFNLFAGR 

       130        140        150        160        170        180 
RADGRVKKVL FPLAPEYIGY ADAGLEEDLF VSARPNIELL PEGQFKYHVD FEHLHIGEET 

       190        200        210        220        230        240 
GMICVSRPTN PTGNVITDEE LLKLDALANQ HGIPLVIDNA YGVPFPGIIF SEARPLWNPN 

       250        260        270        280        290        300 
IVLCMSLSKL GLPGSRCGII IANEKIITAI TNMNGIISLA PGGIGPAMMC EMIKRNDLLR 

       310        320        330        340        350        360 
LSETVIKPFY YQRVQETIAI IRRYLPENRC LIHKPEGAIF LWLWFKDLPI TTKQLYQRLK 

       370        380        390        400        410 
ARGVLMVPGH NFFPGLDKPW PHTHQCMRMN YVPEPEKIEA GVKILAEEIE RAWAESH 

« Hide

References

« Hide 'large scale' references
[1]"Rapid sequencing of cloned DNA using a transposon for bidirectional priming: sequence of the Escherichia coli K-12 avtA gene."
Liu L., Whalen W., Das A., Berg C.M.
Nucleic Acids Res. 15:9461-9469(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005."
Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.
Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 208.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Transamination in Escherichia coli."
Rudman D., Meister A.
J. Biol. Chem. 200:591-604(1953) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN AMINOTRANSFERASE.
[7]"Gratuitous repression of avtA in Escherichia coli and Salmonella typhimurium."
Whalen W.A., Berg C.M.
J. Bacteriol. 158:571-574(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[8]"Genetics and regulation of the major enzymes of alanine synthesis in Escherichia coli."
Kim S.H., Schneider B.L., Reitzer L.
J. Bacteriol. 192:5304-5311(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN ALANINE BIOSYNTHESIS, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00490 Genomic DNA. Translation: CAA68546.1. Sequence problems.
U00039 Genomic DNA. Translation: AAB18549.1.
U00096 Genomic DNA. Translation: AAT48193.1.
AP009048 Genomic DNA. Translation: BAE77721.1.
PIRS47793.
RefSeqYP_026231.1. NC_000913.3.
YP_491862.1. NC_007779.1.

3D structure databases

ProteinModelPortalP09053.
SMRP09053. Positions 15-415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP09053. 1 interaction.
STRING511145.b3572.

Proteomic databases

PaxDbP09053.
PRIDEP09053.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT48193; AAT48193; b3572.
BAE77721; BAE77721; BAE77721.
GeneID12930377.
948087.
KEGGecj:Y75_p3603.
eco:b3572.
PATRIC32122620. VBIEscCol129921_3687.

Organism-specific databases

EchoBASEEB0105.
EcoGeneEG10107. avtA.

Phylogenomic databases

eggNOGCOG3977.
HOGENOMHOG000269357.
KOK00835.
OMAHQCLRMN.
OrthoDBEOG6QCD62.
PhylomeDBP09053.

Enzyme and pathway databases

BioCycEcoCyc:VALINE-PYRUVATE-AMINOTRANSFER-MONOMER.
ECOL316407:JW5652-MONOMER.
MetaCyc:VALINE-PYRUVATE-AMINOTRANSFER-MONOMER.

Gene expression databases

GenevestigatorP09053.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Other

PROP09053.

Entry information

Entry nameAVTA_ECOLI
AccessionPrimary (citable) accession number: P09053
Secondary accession number(s): Q2M7N5, Q6BF21
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene