ID   G3PB_TOBAC              Reviewed;         438 AA.
AC   P09044;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   16-JUN-2009, entry version 74.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic;
DE            EC=1.2.1.13;
DE   AltName: Full=NADP-dependent glyceraldehydephosphate dehydrogenase subunit B;
DE   Flags: Precursor; Fragment;
GN   Name=GAPB;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=87002494; PubMed=3757034; DOI=10.1016/0092-8674(86)90367-3;
RA   Shih M.-C., Lazar G., Goodman H.M.;
RT   "Evidence in favor of the symbiotic origin of chloroplasts: primary
RT   structure and evolution of tobacco glyceraldehyde-3-phosphate
RT   dehydrogenases.";
RL   Cell 47:73-80(1986).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NADP(+) = 3-phospho-D-glyceroyl phosphate + NADPH.
CC   -!- PATHWAY: Carbohydrate biosynthesis; Calvin cycle.
CC   -!- SUBUNIT: Tetramer of either four A chains (GAPDH 2) or two A and
CC       two B chains (GAPDH 1) (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic
CC       form which participates in glycolysis and two chloroplast forms
CC       which participates in photosynthesis. These three forms are
CC       encoded by distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; M14418; AAA34076.1; -; mRNA.
DR   PIR; B24430; B24430.
DR   HSSP; P19866; 1NBO.
DR   SMR; P09044; 54-391.
DR   BRENDA; 1.2.1.13; 298.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0047100; F:glyceraldehyde-3-phosphate dehydrogenase (N...; IEA:EC.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Calvin cycle; Chloroplast; NADP; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT      <1     53       Chloroplast.
FT   CHAIN        54    438       Glyceraldehyde-3-phosphate dehydrogenase
FT                                B, chloroplastic.
FT                                /FTId=PRO_0000010428.
FT   NP_BIND      64     65       NADP (By similarity).
FT   REGION      207    209       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      266    267       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    208    208       Nucleophile (By similarity).
FT   BINDING      88     88       NADP (By similarity).
FT   BINDING     133    133       NADP; via carbonyl oxygen (By
FT                                similarity).
FT   BINDING     238    238       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     253    253       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     289    289       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     372    372       NADP (By similarity).
FT   SITE        235    235       Activates thiol group during catalysis
FT                                (By similarity).
FT   NON_TER       1      1
SQ   SEQUENCE   438 AA;  47470 MW;  7E8DB0F68F206B09 CRC64;
     CLSKKFEVAE FAGLRSSGCV TFSNKESSFF DVVSAQLTPK TTRSTPVKGE TVAKLKVAIN
     GFGRIGRNFL RCWHGRKDSP LDVVVVNDSG GVKNASHLLK YDSMLGTFKA DVKIVDNETI
     SVDGKHIKVV SSRDPLKLPW AELGIDIVIE GTGVFVDGPG AGKHIQAGAK KVIITAPAKG
     ADIPTYVVGV NEQDYSHEVA DIISNASCTT NCLAPFVKVM DEELGIVKGT MTTTHSYTGD
     QRLLDASHRD LRRARAAALN IVPTSTGAAK AVSLVLPQLK GKLNGIALRV PTPNVSVVDL
     VVNVAKKGIT AEDVNAAFRK AADGPLKGVL AVCDEPLVSV DFRCSDVSST IDSSLTMVMG
     DDMVKVVAWY DNEWGYSQRV VDLAHLVANN WPGSCSTRKW RSHWMSFARQ ILLMRNAKSM
     NNRFCYVSSF EMDDFRSQ
//