Reviewed, UniProtKB/Swiss-Prot P09044 (G3PB_TOBAC)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase subunit B

Gene names

Name:

GAPB

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	438 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity.
Subcellular location	Plastid › chloroplast.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 53	›53	Chloroplast
	Chain	54 – 438	385	Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic		PRO_0000010428
Regions
	Nucleotide binding	64 – 65	2	NADP By similarity
	Region	207 – 209	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	266 – 267	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	208	1	Nucleophile By similarity
	Binding site	88	1	NADP By similarity
	Binding site	133	1	NADP; via carbonyl oxygen By similarity
	Binding site	238	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	253	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	289	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	372	1	NADP By similarity
	Site	235	1	Activates thiol group during catalysis By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P09044-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 7E8DB0F68F206B09	FASTA	438	47,470
	10 20 30 40 50 60 CLSKKFEVAE FAGLRSSGCV TFSNKESSFF DVVSAQLTPK TTRSTPVKGE TVAKLKVAIN 70 80 90 100 110 120 GFGRIGRNFL RCWHGRKDSP LDVVVVNDSG GVKNASHLLK YDSMLGTFKA DVKIVDNETI 130 140 150 160 170 180 SVDGKHIKVV SSRDPLKLPW AELGIDIVIE GTGVFVDGPG AGKHIQAGAK KVIITAPAKG 190 200 210 220 230 240 ADIPTYVVGV NEQDYSHEVA DIISNASCTT NCLAPFVKVM DEELGIVKGT MTTTHSYTGD 250 260 270 280 290 300 QRLLDASHRD LRRARAAALN IVPTSTGAAK AVSLVLPQLK GKLNGIALRV PTPNVSVVDL 310 320 330 340 350 360 VVNVAKKGIT AEDVNAAFRK AADGPLKGVL AVCDEPLVSV DFRCSDVSST IDSSLTMVMG 370 380 390 400 410 420 DDMVKVVAWY DNEWGYSQRV VDLAHLVANN WPGSCSTRKW RSHWMSFARQ ILLMRNAKSM 430 NNRFCYVSSF EMDDFRSQ

References

[1]	"Evidence in favor of the symbiotic origin of chloroplasts: primary structure and evolution of tobacco glyceraldehyde-3-phosphate dehydrogenases." Shih M.-C., Lazar G., Goodman H.M. Cell 47:73-80(1986) [PubMed: 3757034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M14418 mRNA. Translation: AAA34076.1.
PIR	B24430.
3D structure databases
HSSP	HSSP built from PDB template 1NBO based on UniProtKB P19866.
SMR	P09044. Positions 54-391.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.13. 298.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PB_TOBAC

Accession

Primary (citable) accession number: P09044

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)