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P09044 (G3PB_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic

EC=1.2.1.13
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit B
Gene names
Name:GAPB
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length438 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathway

Carbohydrate biosynthesis; Calvin cycle.

Subunit structure

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity.

Subcellular location

Plastidchloroplast.

Miscellaneous

Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide‹1 – 53›53Chloroplast
Chain54 – 438385Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic
PRO_0000010428

Regions

Nucleotide binding64 – 652NADP By similarity
Region207 – 2093Glyceraldehyde 3-phosphate binding By similarity
Region266 – 2672Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site2081Nucleophile By similarity
Binding site881NADP By similarity
Binding site1331NADP; via carbonyl oxygen By similarity
Binding site2381Glyceraldehyde 3-phosphate By similarity
Binding site2531Glyceraldehyde 3-phosphate By similarity
Binding site2891Glyceraldehyde 3-phosphate By similarity
Binding site3721NADP By similarity
Site2351Activates thiol group during catalysis By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P09044 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 7E8DB0F68F206B09

FASTA43847,470
        10         20         30         40         50         60 
CLSKKFEVAE FAGLRSSGCV TFSNKESSFF DVVSAQLTPK TTRSTPVKGE TVAKLKVAIN 

        70         80         90        100        110        120 
GFGRIGRNFL RCWHGRKDSP LDVVVVNDSG GVKNASHLLK YDSMLGTFKA DVKIVDNETI 

       130        140        150        160        170        180 
SVDGKHIKVV SSRDPLKLPW AELGIDIVIE GTGVFVDGPG AGKHIQAGAK KVIITAPAKG 

       190        200        210        220        230        240 
ADIPTYVVGV NEQDYSHEVA DIISNASCTT NCLAPFVKVM DEELGIVKGT MTTTHSYTGD 

       250        260        270        280        290        300 
QRLLDASHRD LRRARAAALN IVPTSTGAAK AVSLVLPQLK GKLNGIALRV PTPNVSVVDL 

       310        320        330        340        350        360 
VVNVAKKGIT AEDVNAAFRK AADGPLKGVL AVCDEPLVSV DFRCSDVSST IDSSLTMVMG 

       370        380        390        400        410        420 
DDMVKVVAWY DNEWGYSQRV VDLAHLVANN WPGSCSTRKW RSHWMSFARQ ILLMRNAKSM 

       430 
NNRFCYVSSF EMDDFRSQ 

« Hide

References

[1]"Evidence in favor of the symbiotic origin of chloroplasts: primary structure and evolution of tobacco glyceraldehyde-3-phosphate dehydrogenases."
Shih M.-C., Lazar G., Goodman H.M.
Cell 47:73-80(1986) [PubMed: 3757034] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M14418 mRNA. Translation: AAA34076.1.
PIRB24430.

3D structure databases

ProteinModelPortalP09044.
SMRP09044. Positions 54-393.
ModBaseSearch...

Proteomic databases

PRIDEP09044.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSPR00078. G3PDHDRGNASE.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3PB_TOBAC
AccessionPrimary (citable) accession number: P09044
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: December 14, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families