ID   G3PA_TOBAC              Reviewed;         392 AA.
AC   P09043;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   16-JUN-2009, entry version 75.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic;
DE            EC=1.2.1.13;
DE   AltName: Full=NADP-dependent glyceraldehydephosphate dehydrogenase subunit A;
DE   Flags: Precursor; Fragment;
GN   Name=GAPA;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=87002494; PubMed=3757034; DOI=10.1016/0092-8674(86)90367-3;
RA   Shih M.-C., Lazar G., Goodman H.M.;
RT   "Evidence in favor of the symbiotic origin of chloroplasts: primary
RT   structure and evolution of tobacco glyceraldehyde-3-phosphate
RT   dehydrogenases.";
RL   Cell 47:73-80(1986).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NADP(+) = 3-phospho-D-glyceroyl phosphate + NADPH.
CC   -!- PATHWAY: Carbohydrate biosynthesis; Calvin cycle.
CC   -!- SUBUNIT: Tetramer of either four A chains (GAPDH 2) or two A and
CC       two B chains (GAPDH 1) (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic
CC       form which participates in glycolysis and two chloroplast forms
CC       which participates in photosynthesis. These three forms are
CC       encoded by distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; M14417; AAA34075.1; -; mRNA.
DR   PIR; A24430; A24430.
DR   HSSP; P19866; 1NBO.
DR   SMR; P09043; 57-392.
DR   BRENDA; 1.2.1.13; 298.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0047100; F:glyceraldehyde-3-phosphate dehydrogenase (N...; IEA:EC.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Calvin cycle; Chloroplast; NADP; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT      <1     56       Chloroplast.
FT   CHAIN        57    392       Glyceraldehyde-3-phosphate dehydrogenase
FT                                A, chloroplastic.
FT                                /FTId=PRO_0000010427.
FT   NP_BIND      67     68       NADP (By similarity).
FT   REGION      208    210       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      267    268       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    209    209       Nucleophile (By similarity).
FT   BINDING      91     91       NADP (By similarity).
FT   BINDING     136    136       NADP; via carbonyl oxygen (By
FT                                similarity).
FT   BINDING     239    239       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     254    254       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     290    290       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     372    372       NADP (By similarity).
FT   SITE        236    236       Activates thiol group during catalysis
FT                                (By similarity).
FT   NON_TER       1      1
SQ   SEQUENCE   392 AA;  41863 MW;  35647D90B542D047 CRC64;
     NSSLQVSNKG FSEFSGLRTS SAIPFGRKTN DDLLSVVAFQ TSVIGGGNSK RGVVEAKLKV
     AINGFGRIGR NFLRCWHGRK DSPLDVIAIN DTGGVKQASH LLKYDSTLGI FDADVKPVGT
     DGISVDGKVI QVVSDRNPVN LPWGDLGIDL VIEGTGVFVD REGAGKHIQA GAKKVLITAP
     GKGDIPTYVV GVNADLYNPD EPIISNASCT TNCLAPFVKV LDQKFGIIKG TMTTTHSYTG
     DQRLLDASHR DLRRARAAAL NIVPTSTGAA KAVALSSQAL RGSSMALPLR VPTPNVSVVD
     LVVQVSKKTF AEEVNAAFRE AADKELKGIL DVCDEPLVSV DFRCSDVSST VDASLTMVMG
     DDMVKVIAWY DNEWGYSQRV VDLADIVANQ WK
//