Reviewed, UniProtKB/Swiss-Prot P09043 (G3PA_TOBAC)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase subunit A

Gene names

Name:

GAPA

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	392 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity.
Subcellular location	Plastid › chloroplast.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 56	›56	Chloroplast
	Chain	57 – 392	336	Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic		PRO_0000010427
Regions
	Nucleotide binding	67 – 68	2	NADP By similarity
	Region	208 – 210	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	267 – 268	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	209	1	Nucleophile By similarity
	Binding site	91	1	NADP By similarity
	Binding site	136	1	NADP; via carbonyl oxygen By similarity
	Binding site	239	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	254	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	290	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	372	1	NADP By similarity
	Site	236	1	Activates thiol group during catalysis By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P09043-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 35647D90B542D047	FASTA	392	41,863
	10 20 30 40 50 60 NSSLQVSNKG FSEFSGLRTS SAIPFGRKTN DDLLSVVAFQ TSVIGGGNSK RGVVEAKLKV 70 80 90 100 110 120 AINGFGRIGR NFLRCWHGRK DSPLDVIAIN DTGGVKQASH LLKYDSTLGI FDADVKPVGT 130 140 150 160 170 180 DGISVDGKVI QVVSDRNPVN LPWGDLGIDL VIEGTGVFVD REGAGKHIQA GAKKVLITAP 190 200 210 220 230 240 GKGDIPTYVV GVNADLYNPD EPIISNASCT TNCLAPFVKV LDQKFGIIKG TMTTTHSYTG 250 260 270 280 290 300 DQRLLDASHR DLRRARAAAL NIVPTSTGAA KAVALSSQAL RGSSMALPLR VPTPNVSVVD 310 320 330 340 350 360 LVVQVSKKTF AEEVNAAFRE AADKELKGIL DVCDEPLVSV DFRCSDVSST VDASLTMVMG 370 380 390 DDMVKVIAWY DNEWGYSQRV VDLADIVANQ WK

References

[1]	"Evidence in favor of the symbiotic origin of chloroplasts: primary structure and evolution of tobacco glyceraldehyde-3-phosphate dehydrogenases." Shih M.-C., Lazar G., Goodman H.M. Cell 47:73-80(1986) [PubMed: 3757034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M14417 mRNA. Translation: AAA34075.1.
PIR	A24430.
3D structure databases
HSSP	HSSP built from PDB template 1NBO based on UniProtKB P19866.
SMR	P09043. Positions 57-392.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.13. 298.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PA_TOBAC

Accession

Primary (citable) accession number: P09043

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)