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Protein

Phosphoglycerate kinase 2

Gene

Pgk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for sperm motility and male fertility but is not required for the completion of spermatogenesis (PubMed:19759366).1 Publication

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm7293), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm3839)
  2. Phosphoglycerate kinase 2 (Pgk2), Phosphoglycerate kinase 1 (Pgk1)
  3. no protein annotated in this organism
  4. Gamma-enolase (Eno2), Alpha-enolase (Eno1), Beta-enolase (Eno3), Enolase 4 (Eno4)
  5. Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKLR (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKM (Pkm)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Substrate1
Binding sitei123Substrate1
Binding sitei171Substrate1
Binding sitei220ATP1 Publication1
Binding sitei313ATP; via carbonyl oxygen1 Publication1
Binding sitei344ATP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi373 – 376ATP1 Publication4

GO - Molecular functioni

GO - Biological processi

  • flagellated sperm motility Source: MGI
  • glycolytic process Source: MGI
  • mitophagy in response to mitochondrial depolarization Source: Ensembl
  • phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.2.3. 3474.
SABIO-RKP09041.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 2 (EC:2.7.2.3)
Alternative name(s):
Phosphoglycerate kinase, testis specific
Gene namesi
Name:Pgk2
Synonyms:Pgk-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:97563. Pgk2.

Subcellular locationi

GO - Cellular componenti

  • cilium Source: MGI
  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • nucleus Source: MGI
  • sperm fibrous sheath Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice display greatly reduced ATP levels in sperm, severely impaired sperm motility and are infertile. No alteration in testis histology, sperm counts, or sperm ultrastructure seen.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001458362 – 417Phosphoglycerate kinase 2Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei6N6-succinyllysineBy similarity1
Modified residuei11N6-acetyllysineBy similarity1
Modified residuei48N6-acetyllysine; alternateBy similarity1
Modified residuei48N6-succinyllysine; alternateBy similarity1
Modified residuei75N6-acetyllysineBy similarity1
Modified residuei76PhosphotyrosineBy similarity1
Modified residuei86N6-acetyllysineBy similarity1
Modified residuei91N6-acetyllysineBy similarity1
Modified residuei97N6-acetyllysineBy similarity1
Modified residuei131N6-acetyllysineBy similarity1
Modified residuei146N6-acetyllysineBy similarity1
Modified residuei191N6-succinyllysineBy similarity1
Modified residuei196PhosphotyrosineBy similarity1
Modified residuei199N6-acetyllysineBy similarity1
Modified residuei267N6-acetyllysineBy similarity1
Modified residuei291N6-acetyllysineBy similarity1
Modified residuei361N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP09041.
MaxQBiP09041.
PaxDbiP09041.
PeptideAtlasiP09041.
PRIDEiP09041.

2D gel databases

REPRODUCTION-2DPAGEIPI00555060.
P09041.

PTM databases

iPTMnetiP09041.
PhosphoSitePlusiP09041.
SwissPalmiP09041.

Expressioni

Tissue specificityi

Testis and sperm. Localized on the principle piece in the sperm (at protein level). Testis-specific.2 Publications

Gene expression databases

BgeeiENSMUSG00000031233.
CleanExiMM_PGK2.
GenevisibleiP09041. MM.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiP09041. 1 interactor.
STRINGi10090.ENSMUSP00000033585.

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Beta strandi18 – 22Combined sources5
Beta strandi29 – 32Combined sources4
Beta strandi33 – 35Combined sources3
Helixi38 – 52Combined sources15
Beta strandi56 – 61Combined sources6
Helixi73 – 76Combined sources4
Helixi79 – 89Combined sources11
Beta strandi93 – 95Combined sources3
Beta strandi99 – 101Combined sources3
Helixi102 – 109Combined sources8
Beta strandi115 – 118Combined sources4
Helixi122 – 124Combined sources3
Turni126 – 130Combined sources5
Beta strandi131 – 133Combined sources3
Beta strandi139 – 141Combined sources3
Helixi144 – 155Combined sources12
Beta strandi159 – 163Combined sources5
Helixi166 – 168Combined sources3
Helixi174 – 177Combined sources4
Beta strandi184 – 186Combined sources3
Helixi188 – 202Combined sources15
Beta strandi206 – 213Combined sources8
Helixi218 – 220Combined sources3
Helixi221 – 227Combined sources7
Turni228 – 230Combined sources3
Beta strandi232 – 237Combined sources6
Helixi240 – 249Combined sources10
Beta strandi253 – 256Combined sources4
Helixi260 – 263Combined sources4
Helixi266 – 275Combined sources10
Beta strandi279 – 281Combined sources3
Beta strandi284 – 293Combined sources10
Beta strandi298 – 302Combined sources5
Turni303 – 305Combined sources3
Beta strandi312 – 316Combined sources5
Helixi318 – 329Combined sources12
Beta strandi332 – 338Combined sources7
Helixi346 – 348Combined sources3
Helixi350 – 364Combined sources15
Beta strandi368 – 371Combined sources4
Helixi375 – 382Combined sources8
Turni386 – 388Combined sources3
Beta strandi389 – 392Combined sources4
Helixi396 – 402Combined sources7
Turni403 – 405Combined sources3
Helixi409 – 412Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P9QX-ray2.70A/B2-417[»]
2P9TX-ray2.00A2-417[»]
2PAAX-ray2.70A/B2-417[»]
ProteinModelPortaliP09041.
SMRiP09041.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09041.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 26Substrate binding3
Regioni63 – 66Substrate binding4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
GeneTreeiENSGT00390000008820.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiP09041.
KOiK00927.
OMAiCAAKYNT.
OrthoDBiEOG091G08S6.
TreeFamiTF300489.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSAKLTLD KVDLKGKRVI MRVDFNVPMK NNQITNNQRI KAAIPSIKHC
60 70 80 90 100
LDNGAKSVVL MSHLGRPDGI PMPDKYSLEP VADELKSLLN KDVIFLKDCV
110 120 130 140 150
GPEVEQACAN PDNGSIILLE NLRFHVEEEG KGKDSSGKKI SADPAKVEAF
160 170 180 190 200
QASLSKLGDV YVNDAFGTAH RAHSSTVGVN LPQKASGFLM KKELDYFSKA
210 220 230 240 250
LEKPERPFLA ILGGAKVKDK IQLIKNMLDK VNFMIIGGGM AYTFLKELKN
260 270 280 290 300
MQIGASLFDE EGATIVKEIM EKAEKNGVKI VFPVDFVTGD KFDENAKVGQ
310 320 330 340 350
ATIESGIPSG WMGLDCGPES IKINAQIVAQ AKLIVWNGPI GVFEWDAFAK
360 370 380 390 400
GTKALMDEVV KATSNGCVTI IGGGDTATCC AKWGTEDKVS HVSTGGGASL
410
ELLEGKILPG VEALSNM
Length:417
Mass (Da):44,853
Last modified:July 27, 2011 - v4
Checksum:iBC3FACE559798B53
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151Q → R in AAA39920 (PubMed:2823118).Curated1
Sequence conflicti176T → M in AAA39920 (PubMed:2823118).Curated1
Sequence conflicti176T → M in AAA39921 (PubMed:2823118).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17299 Genomic DNA. Translation: AAA39920.1.
M18654 mRNA. Translation: AAA39921.1.
AK133436 mRNA. Translation: BAE21656.1.
CH466559 Genomic DNA. Translation: EDL23381.1.
BC052343 mRNA. Translation: AAH52343.1.
BC061054 mRNA. Translation: AAH61054.1.
X55310 Genomic DNA. Translation: CAA39014.1.
CCDSiCCDS28782.1.
PIRiA27775.
RefSeqiNP_112467.2. NM_031190.2.
UniGeneiMm.717.

Genome annotation databases

EnsembliENSMUST00000033585; ENSMUSP00000033585; ENSMUSG00000031233.
GeneIDi18663.
KEGGimmu:18663.
UCSCiuc008cof.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17299 Genomic DNA. Translation: AAA39920.1.
M18654 mRNA. Translation: AAA39921.1.
AK133436 mRNA. Translation: BAE21656.1.
CH466559 Genomic DNA. Translation: EDL23381.1.
BC052343 mRNA. Translation: AAH52343.1.
BC061054 mRNA. Translation: AAH61054.1.
X55310 Genomic DNA. Translation: CAA39014.1.
CCDSiCCDS28782.1.
PIRiA27775.
RefSeqiNP_112467.2. NM_031190.2.
UniGeneiMm.717.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P9QX-ray2.70A/B2-417[»]
2P9TX-ray2.00A2-417[»]
2PAAX-ray2.70A/B2-417[»]
ProteinModelPortaliP09041.
SMRiP09041.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09041. 1 interactor.
STRINGi10090.ENSMUSP00000033585.

PTM databases

iPTMnetiP09041.
PhosphoSitePlusiP09041.
SwissPalmiP09041.

2D gel databases

REPRODUCTION-2DPAGEIPI00555060.
P09041.

Proteomic databases

EPDiP09041.
MaxQBiP09041.
PaxDbiP09041.
PeptideAtlasiP09041.
PRIDEiP09041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033585; ENSMUSP00000033585; ENSMUSG00000031233.
GeneIDi18663.
KEGGimmu:18663.
UCSCiuc008cof.2. mouse.

Organism-specific databases

CTDi5232.
MGIiMGI:97563. Pgk2.

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
GeneTreeiENSGT00390000008820.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiP09041.
KOiK00927.
OMAiCAAKYNT.
OrthoDBiEOG091G08S6.
TreeFamiTF300489.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BRENDAi2.7.2.3. 3474.
SABIO-RKP09041.

Miscellaneous databases

EvolutionaryTraceiP09041.
PROiP09041.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031233.
CleanExiMM_PGK2.
GenevisibleiP09041. MM.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK2_MOUSE
AccessioniPrimary (citable) accession number: P09041
Secondary accession number(s): Q5RKV3, Q6P8V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 139 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.