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P09041

- PGK2_MOUSE

UniProt

P09041 - PGK2_MOUSE

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Protein

Phosphoglycerate kinase 2

Gene

Pgk2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391Substrate
Binding sitei123 – 1231Substrate
Binding sitei171 – 1711Substrate
Binding sitei220 – 2201ATP1 Publication
Binding sitei313 – 3131ATP; via carbonyl oxygen1 Publication
Binding sitei344 – 3441ATP1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi373 – 3764ATP1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. phosphoglycerate kinase activity Source: UniProtKB

GO - Biological processi

  1. glycolytic process Source: MGI
  2. phosphorylation Source: UniProtKB
  3. sperm motility Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP09041.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 2 (EC:2.7.2.3)
Alternative name(s):
Phosphoglycerate kinase, testis specific
Gene namesi
Name:Pgk2
Synonyms:Pgk-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:97563. Pgk2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cilium Source: MGI
  2. cytoplasm Source: UniProtKB-KW
  3. nucleus Source: Ensembl
  4. sperm fibrous sheath Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Phosphoglycerate kinase 2PRO_0000145836Add
BLAST

Proteomic databases

MaxQBiP09041.
PaxDbiP09041.
PRIDEiP09041.

2D gel databases

REPRODUCTION-2DPAGEIPI00555060.
P09041.

PTM databases

PhosphoSiteiP09041.

Expressioni

Gene expression databases

BgeeiP09041.
CleanExiMM_PGK2.
GenevestigatoriP09041.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiP09041. 1 interaction.

Structurei

Secondary structure

1
417
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113
Beta strandi18 – 225
Beta strandi29 – 324
Beta strandi33 – 353
Helixi38 – 5215
Beta strandi56 – 616
Helixi73 – 764
Helixi79 – 8911
Beta strandi93 – 953
Beta strandi99 – 1013
Helixi102 – 1098
Beta strandi115 – 1184
Helixi122 – 1243
Turni126 – 1305
Beta strandi131 – 1333
Beta strandi139 – 1413
Helixi144 – 15512
Beta strandi159 – 1635
Helixi166 – 1683
Helixi174 – 1774
Beta strandi184 – 1863
Helixi188 – 20215
Beta strandi206 – 2138
Helixi218 – 2203
Helixi221 – 2277
Turni228 – 2303
Beta strandi232 – 2376
Helixi240 – 24910
Beta strandi253 – 2564
Helixi260 – 2634
Helixi266 – 27510
Beta strandi279 – 2813
Beta strandi284 – 29310
Beta strandi298 – 3025
Turni303 – 3053
Beta strandi312 – 3165
Helixi318 – 32912
Beta strandi332 – 3387
Helixi346 – 3483
Helixi350 – 36415
Beta strandi368 – 3714
Helixi375 – 3828
Turni386 – 3883
Beta strandi389 – 3924
Helixi396 – 4027
Turni403 – 4053
Helixi409 – 4124

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P9QX-ray2.70A/B2-417[»]
2P9TX-ray2.00A2-417[»]
2PAAX-ray2.70A/B2-417[»]
ProteinModelPortaliP09041.
SMRiP09041. Positions 5-417.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09041.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 263Substrate binding
Regioni63 – 664Substrate binding

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0126.
GeneTreeiENSGT00390000008820.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiP09041.
KOiK00927.
OMAiVAKEFAP.
OrthoDBiEOG74R1QN.
TreeFamiTF300489.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09041-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALSAKLTLD KVDLKGKRVI MRVDFNVPMK NNQITNNQRI KAAIPSIKHC
60 70 80 90 100
LDNGAKSVVL MSHLGRPDGI PMPDKYSLEP VADELKSLLN KDVIFLKDCV
110 120 130 140 150
GPEVEQACAN PDNGSIILLE NLRFHVEEEG KGKDSSGKKI SADPAKVEAF
160 170 180 190 200
QASLSKLGDV YVNDAFGTAH RAHSSTVGVN LPQKASGFLM KKELDYFSKA
210 220 230 240 250
LEKPERPFLA ILGGAKVKDK IQLIKNMLDK VNFMIIGGGM AYTFLKELKN
260 270 280 290 300
MQIGASLFDE EGATIVKEIM EKAEKNGVKI VFPVDFVTGD KFDENAKVGQ
310 320 330 340 350
ATIESGIPSG WMGLDCGPES IKINAQIVAQ AKLIVWNGPI GVFEWDAFAK
360 370 380 390 400
GTKALMDEVV KATSNGCVTI IGGGDTATCC AKWGTEDKVS HVSTGGGASL
410
ELLEGKILPG VEALSNM
Length:417
Mass (Da):44,853
Last modified:July 27, 2011 - v4
Checksum:iBC3FACE559798B53
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511Q → R in AAA39920. (PubMed:2823118)Curated
Sequence conflicti176 – 1761T → M in AAA39920. (PubMed:2823118)Curated
Sequence conflicti176 – 1761T → M in AAA39921. (PubMed:2823118)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17299 Genomic DNA. Translation: AAA39920.1.
M18654 mRNA. Translation: AAA39921.1.
AK133436 mRNA. Translation: BAE21656.1.
CH466559 Genomic DNA. Translation: EDL23381.1.
BC052343 mRNA. Translation: AAH52343.1.
BC061054 mRNA. Translation: AAH61054.1.
X55310 Genomic DNA. Translation: CAA39014.1.
CCDSiCCDS28782.1.
PIRiA27775.
RefSeqiNP_112467.2. NM_031190.2.
UniGeneiMm.717.

Genome annotation databases

EnsembliENSMUST00000033585; ENSMUSP00000033585; ENSMUSG00000031233.
GeneIDi18663.
KEGGimmu:18663.
UCSCiuc008cof.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17299 Genomic DNA. Translation: AAA39920.1 .
M18654 mRNA. Translation: AAA39921.1 .
AK133436 mRNA. Translation: BAE21656.1 .
CH466559 Genomic DNA. Translation: EDL23381.1 .
BC052343 mRNA. Translation: AAH52343.1 .
BC061054 mRNA. Translation: AAH61054.1 .
X55310 Genomic DNA. Translation: CAA39014.1 .
CCDSi CCDS28782.1.
PIRi A27775.
RefSeqi NP_112467.2. NM_031190.2.
UniGenei Mm.717.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2P9Q X-ray 2.70 A/B 2-417 [» ]
2P9T X-ray 2.00 A 2-417 [» ]
2PAA X-ray 2.70 A/B 2-417 [» ]
ProteinModelPortali P09041.
SMRi P09041. Positions 5-417.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P09041. 1 interaction.

PTM databases

PhosphoSitei P09041.

2D gel databases

REPRODUCTION-2DPAGE IPI00555060.
P09041.

Proteomic databases

MaxQBi P09041.
PaxDbi P09041.
PRIDEi P09041.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033585 ; ENSMUSP00000033585 ; ENSMUSG00000031233 .
GeneIDi 18663.
KEGGi mmu:18663.
UCSCi uc008cof.2. mouse.

Organism-specific databases

CTDi 5232.
MGIi MGI:97563. Pgk2.

Phylogenomic databases

eggNOGi COG0126.
GeneTreei ENSGT00390000008820.
HOGENOMi HOG000227107.
HOVERGENi HBG008177.
InParanoidi P09041.
KOi K00927.
OMAi VAKEFAP.
OrthoDBi EOG74R1QN.
TreeFami TF300489.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00185 .
SABIO-RK P09041.

Miscellaneous databases

EvolutionaryTracei P09041.
NextBioi 294668.
PROi P09041.
SOURCEi Search...

Gene expression databases

Bgeei P09041.
CleanExi MM_PGK2.
Genevestigatori P09041.

Family and domain databases

Gene3Di 3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPi MF_00145. Phosphoglyc_kinase.
InterProi IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view ]
PANTHERi PTHR11406. PTHR11406. 1 hit.
Pfami PF00162. PGK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000724. Pgk. 1 hit.
PRINTSi PR00477. PHGLYCKINASE.
SUPFAMi SSF53748. SSF53748. 1 hit.
PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The testis-specific phosphoglycerate kinase gene pgk-2 is a recruited retroposon."
    Boer P.H., Adra C.N., Lau Y.-F.C., McBurney M.W.
    Mol. Cell. Biol. 7:3107-3112(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  5. "Selective activation of testis-specific genes in cultured rat spermatogenic cells."
    Tamaru M., Nagao Y., Taira M., Tatibana M., Masamune Y., Nakanishi Y.
    Biochim. Biophys. Acta 1049:331-338(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
  6. "X-ray analysis of phosphoglycerate kinase 2, a sperm-specific isoform from Mus musculus."
    Sawyer G.M., Monzingo A.F., Poteet E.C., O'Brien D.A., Robertus J.D.
    Proteins 71:1134-1144(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-417 ALONE AND IN COMPLEX WITH PHOSPHOGLYCERATE AND ATP, SUBUNIT, SUBSTRATE-BINDING SITES.

Entry informationi

Entry nameiPGK2_MOUSE
AccessioniPrimary (citable) accession number: P09041
Secondary accession number(s): Q5RKV3, Q6P8V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 119 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3