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P09036

- ISK3_MOUSE

UniProt

P09036 - ISK3_MOUSE

Protein

Serine protease inhibitor Kazal-type 3

Gene

Spink3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Serine protease inhibitor which exhibits anti-trypsin activity. Inhibits the uptake of calcium by spermatozoa.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei42 – 432Reactive bond

    GO - Molecular functioni

    1. peptidase inhibitor activity Source: MGI
    2. serine-type endopeptidase inhibitor activity Source: MGI

    GO - Biological processi

    1. negative regulation of calcium ion import Source: MGI
    2. negative regulation of nitric oxide mediated signal transduction Source: MGI
    3. negative regulation of peptidase activity Source: MGI
    4. negative regulation of peptidyl-tyrosine phosphorylation Source: MGI
    5. negative regulation of serine-type endopeptidase activity Source: MGI
    6. regulation of acrosome reaction Source: MGI
    7. regulation of store-operated calcium entry Source: MGI

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI01.011.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine protease inhibitor Kazal-type 3
    Alternative name(s):
    P12
    Prostatic secretory glycoprotein
    Gene namesi
    Name:Spink3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:106202. Spink3.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 8057Serine protease inhibitor Kazal-type 3PRO_0000016568Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 62PROSITE-ProRule annotation
    Disulfide bondi40 ↔ 59PROSITE-ProRule annotation
    Disulfide bondi48 ↔ 80PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP09036.
    PRIDEiP09036.

    Expressioni

    Tissue specificityi

    In the genital tract, expressed only in male accessory glands including seminal vesicle, coagulating gland and prostate.1 Publication

    Developmental stagei

    Not expressed during prepubertal stages. Expression coincides with maturation.1 Publication

    Inductioni

    By androgens in adult male sex accessory glands but expressed constitutively in pancreas.1 Publication

    Gene expression databases

    BgeeiP09036.
    CleanExiMM_SPINK3.
    GenevestigatoriP09036.

    Interactioni

    Protein-protein interaction databases

    IntActiP09036. 1 interaction.
    MINTiMINT-4098780.
    STRINGi10090.ENSMUSP00000025381.

    Structurei

    3D structure databases

    ProteinModelPortaliP09036.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 8054Kazal-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Kazal-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG320895.
    GeneTreeiENSGT00530000064228.
    HOGENOMiHOG000090244.
    HOVERGENiHBG006182.
    InParanoidiP09036.
    OrthoDBiEOG7BW0M2.
    PhylomeDBiP09036.

    Family and domain databases

    InterProiIPR002350. Kazal_dom.
    IPR001239. Prot_inh_Kazal-m.
    [Graphical view]
    PfamiPF00050. Kazal_1. 1 hit.
    [Graphical view]
    PRINTSiPR00290. KAZALINHBTR.
    SMARTiSM00280. KAZAL. 1 hit.
    [Graphical view]
    PROSITEiPS00282. KAZAL_1. 1 hit.
    PS51465. KAZAL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09036-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVAVIFLLS ALALLSLAGN TFSAKVTGKE ASCHDAVAGC PRIYDPVCGT   50
    DGITYANECV LCFENRKRIE PVLIRKGGPC 80
    Length:80
    Mass (Da):8,488
    Last modified:November 1, 1988 - v1
    Checksum:i4DC1F2EC4804CCA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06342 mRNA. Translation: CAA29648.1.
    AK007841 mRNA. Translation: BAB25298.1.
    AK007985 mRNA. Translation: BAB25389.1.
    BC086887 mRNA. Translation: AAH86887.1.
    CCDSiCCDS37803.1.
    PIRiS01498.
    RefSeqiNP_033284.1. NM_009258.5.
    UniGeneiMm.272.

    Genome annotation databases

    EnsembliENSMUST00000025381; ENSMUSP00000025381; ENSMUSG00000024503.
    GeneIDi20730.
    KEGGimmu:20730.
    UCSCiuc008eul.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06342 mRNA. Translation: CAA29648.1 .
    AK007841 mRNA. Translation: BAB25298.1 .
    AK007985 mRNA. Translation: BAB25389.1 .
    BC086887 mRNA. Translation: AAH86887.1 .
    CCDSi CCDS37803.1.
    PIRi S01498.
    RefSeqi NP_033284.1. NM_009258.5.
    UniGenei Mm.272.

    3D structure databases

    ProteinModelPortali P09036.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P09036. 1 interaction.
    MINTi MINT-4098780.
    STRINGi 10090.ENSMUSP00000025381.

    Protein family/group databases

    MEROPSi I01.011.

    Proteomic databases

    PaxDbi P09036.
    PRIDEi P09036.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025381 ; ENSMUSP00000025381 ; ENSMUSG00000024503 .
    GeneIDi 20730.
    KEGGi mmu:20730.
    UCSCi uc008eul.2. mouse.

    Organism-specific databases

    CTDi 20730.
    MGIi MGI:106202. Spink3.

    Phylogenomic databases

    eggNOGi NOG320895.
    GeneTreei ENSGT00530000064228.
    HOGENOMi HOG000090244.
    HOVERGENi HBG006182.
    InParanoidi P09036.
    OrthoDBi EOG7BW0M2.
    PhylomeDBi P09036.

    Miscellaneous databases

    ChiTaRSi SPINK1. mouse.
    NextBioi 299347.
    PROi P09036.
    SOURCEi Search...

    Gene expression databases

    Bgeei P09036.
    CleanExi MM_SPINK3.
    Genevestigatori P09036.

    Family and domain databases

    InterProi IPR002350. Kazal_dom.
    IPR001239. Prot_inh_Kazal-m.
    [Graphical view ]
    Pfami PF00050. Kazal_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00290. KAZALINHBTR.
    SMARTi SM00280. KAZAL. 1 hit.
    [Graphical view ]
    PROSITEi PS00282. KAZAL_1. 1 hit.
    PS51465. KAZAL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A secretory protease inhibitor requires androgens for its expression in male sex accessory tissues but is expressed constitutively in pancreas."
      Mills J.S., Needham M., Parker M.G.
      EMBO J. 6:3711-3717(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
      Strain: C57BL/6.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Pancreas.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    4. "Purification and characterization of a trypsin inhibitor from mouse seminal vesicle secretion."
      Lai M.-L., Chen S.-W., Chen Y.-H.
      Arch. Biochem. Biophys. 290:265-271(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 43-47; 49-61 AND 63-72.
    5. "Developmental profile of a caltrin-like protease inhibitor, P12, in mouse seminal vesicle and characterization of its binding sites on sperm surface."
      Chen L.-Y., Lin Y.-H., Lai M.-L., Chen Y.-H.
      Biol. Reprod. 59:1498-1505(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiISK3_MOUSE
    AccessioniPrimary (citable) accession number: P09036
    Secondary accession number(s): Q5M9M3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3