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Protein

Serine protease inhibitor Kazal-type 3

Gene

Spink3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor which exhibits anti-trypsin activity. Inhibits the uptake of calcium by spermatozoa.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei42 – 432Reactive bond

GO - Molecular functioni

  • peptidase inhibitor activity Source: MGI
  • serine-type endopeptidase inhibitor activity Source: MGI

GO - Biological processi

  • negative regulation of calcium ion import Source: MGI
  • negative regulation of nitric oxide mediated signal transduction Source: MGI
  • negative regulation of peptidase activity Source: MGI
  • negative regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • negative regulation of serine-type endopeptidase activity Source: MGI
  • regulation of acrosome reaction Source: MGI
  • regulation of store-operated calcium entry Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 3
Alternative name(s):
P12
Prostatic secretory glycoprotein
Gene namesi
Name:Spink3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:106202. Spink3.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 8057Serine protease inhibitor Kazal-type 3PRO_0000016568Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 62PROSITE-ProRule annotation
Disulfide bondi40 ↔ 59PROSITE-ProRule annotation
Disulfide bondi48 ↔ 80PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP09036.
PRIDEiP09036.

Expressioni

Tissue specificityi

In the genital tract, expressed only in male accessory glands including seminal vesicle, coagulating gland and prostate.1 Publication

Developmental stagei

Not expressed during prepubertal stages. Expression coincides with maturation.1 Publication

Inductioni

By androgens in adult male sex accessory glands but expressed constitutively in pancreas.1 Publication

Gene expression databases

BgeeiP09036.
CleanExiMM_SPINK3.
GenevestigatoriP09036.

Interactioni

Protein-protein interaction databases

IntActiP09036. 1 interaction.
MINTiMINT-4098780.
STRINGi10090.ENSMUSP00000025381.

Structurei

3D structure databases

ProteinModelPortaliP09036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 8054Kazal-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG320895.
GeneTreeiENSGT00530000064228.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiP09036.
OMAiCTKIYNP.
OrthoDBiEOG7BW0M2.
PhylomeDBiP09036.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
PRINTSiPR00290. KAZALINHBTR.
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAVIFLLS ALALLSLAGN TFSAKVTGKE ASCHDAVAGC PRIYDPVCGT
60 70 80
DGITYANECV LCFENRKRIE PVLIRKGGPC
Length:80
Mass (Da):8,488
Last modified:November 1, 1988 - v1
Checksum:i4DC1F2EC4804CCA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06342 mRNA. Translation: CAA29648.1.
AK007841 mRNA. Translation: BAB25298.1.
AK007985 mRNA. Translation: BAB25389.1.
BC086887 mRNA. Translation: AAH86887.1.
CCDSiCCDS37803.1.
PIRiS01498.
RefSeqiNP_033284.1. NM_009258.5.
UniGeneiMm.272.

Genome annotation databases

EnsembliENSMUST00000025381; ENSMUSP00000025381; ENSMUSG00000024503.
GeneIDi20730.
KEGGimmu:20730.
UCSCiuc008eul.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06342 mRNA. Translation: CAA29648.1.
AK007841 mRNA. Translation: BAB25298.1.
AK007985 mRNA. Translation: BAB25389.1.
BC086887 mRNA. Translation: AAH86887.1.
CCDSiCCDS37803.1.
PIRiS01498.
RefSeqiNP_033284.1. NM_009258.5.
UniGeneiMm.272.

3D structure databases

ProteinModelPortaliP09036.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09036. 1 interaction.
MINTiMINT-4098780.
STRINGi10090.ENSMUSP00000025381.

Protein family/group databases

MEROPSiI01.011.

Proteomic databases

PaxDbiP09036.
PRIDEiP09036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025381; ENSMUSP00000025381; ENSMUSG00000024503.
GeneIDi20730.
KEGGimmu:20730.
UCSCiuc008eul.2. mouse.

Organism-specific databases

CTDi20730.
MGIiMGI:106202. Spink3.

Phylogenomic databases

eggNOGiNOG320895.
GeneTreeiENSGT00530000064228.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiP09036.
OMAiCTKIYNP.
OrthoDBiEOG7BW0M2.
PhylomeDBiP09036.

Miscellaneous databases

NextBioi299347.
PROiP09036.
SOURCEiSearch...

Gene expression databases

BgeeiP09036.
CleanExiMM_SPINK3.
GenevestigatoriP09036.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
PRINTSiPR00290. KAZALINHBTR.
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A secretory protease inhibitor requires androgens for its expression in male sex accessory tissues but is expressed constitutively in pancreas."
    Mills J.S., Needham M., Parker M.G.
    EMBO J. 6:3711-3717(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
    Strain: C57BL/6.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Pancreas.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "Purification and characterization of a trypsin inhibitor from mouse seminal vesicle secretion."
    Lai M.-L., Chen S.-W., Chen Y.-H.
    Arch. Biochem. Biophys. 290:265-271(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 43-47; 49-61 AND 63-72.
  5. "Developmental profile of a caltrin-like protease inhibitor, P12, in mouse seminal vesicle and characterization of its binding sites on sperm surface."
    Chen L.-Y., Lin Y.-H., Lai M.-L., Chen Y.-H.
    Biol. Reprod. 59:1498-1505(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiISK3_MOUSE
AccessioniPrimary (citable) accession number: P09036
Secondary accession number(s): Q5M9M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 27, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.