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P09036 (ISK3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine protease inhibitor Kazal-type 3
Alternative name(s):
P12
Prostatic secretory glycoprotein
Gene names
Name:Spink3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length80 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine protease inhibitor which exhibits anti-trypsin activity. Inhibits the uptake of calcium by spermatozoa. Ref.1 Ref.5

Subcellular location

Secreted.

Tissue specificity

In the genital tract, expressed only in male accessory glands including seminal vesicle, coagulating gland and prostate. Ref.5

Developmental stage

Not expressed during prepubertal stages. Expression coincides with maturation. Ref.5

Induction

By androgens in adult male sex accessory glands but expressed constitutively in pancreas. Ref.1

Sequence similarities

Contains 1 Kazal-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 8057Serine protease inhibitor Kazal-type 3
PRO_0000016568

Regions

Domain27 – 8054Kazal-like

Sites

Site42 – 432Reactive bond

Amino acid modifications

Disulfide bond33 ↔ 62 By similarity
Disulfide bond40 ↔ 59 By similarity
Disulfide bond48 ↔ 80 By similarity

Sequences

Sequence LengthMass (Da)Tools
P09036 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 4DC1F2EC4804CCA6

FASTA808,488
        10         20         30         40         50         60 
MKVAVIFLLS ALALLSLAGN TFSAKVTGKE ASCHDAVAGC PRIYDPVCGT DGITYANECV 

        70         80 
LCFENRKRIE PVLIRKGGPC 

« Hide

References

« Hide 'large scale' references
[1]"A secretory protease inhibitor requires androgens for its expression in male sex accessory tissues but is expressed constitutively in pancreas."
Mills J.S., Needham M., Parker M.G.
EMBO J. 6:3711-3717(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
Strain: C57BL/6.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Pancreas.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[4]"Purification and characterization of a trypsin inhibitor from mouse seminal vesicle secretion."
Lai M.-L., Chen S.-W., Chen Y.-H.
Arch. Biochem. Biophys. 290:265-271(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 43-47; 49-61 AND 63-72.
[5]"Developmental profile of a caltrin-like protease inhibitor, P12, in mouse seminal vesicle and characterization of its binding sites on sperm surface."
Chen L.-Y., Lin Y.-H., Lai M.-L., Chen Y.-H.
Biol. Reprod. 59:1498-1505(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06342 mRNA. Translation: CAA29648.1.
AK007841 mRNA. Translation: BAB25298.1.
AK007985 mRNA. Translation: BAB25389.1.
BC086887 mRNA. Translation: AAH86887.1.
CCDSCCDS37803.1.
PIRS01498.
RefSeqNP_033284.1. NM_009258.5.
UniGeneMm.272.

3D structure databases

ProteinModelPortalP09036.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP09036. 1 interaction.
MINTMINT-4098780.
STRING10090.ENSMUSP00000025381.

Protein family/group databases

MEROPSI01.011.

Proteomic databases

PaxDbP09036.
PRIDEP09036.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025381; ENSMUSP00000025381; ENSMUSG00000024503.
GeneID20730.
KEGGmmu:20730.
UCSCuc008eul.2. mouse.

Organism-specific databases

CTD20730.
MGIMGI:106202. Spink3.

Phylogenomic databases

eggNOGNOG320895.
GeneTreeENSGT00530000064228.
HOGENOMHOG000090244.
HOVERGENHBG006182.
InParanoidP09036.
OrthoDBEOG7BW0M2.
PhylomeDBP09036.

Gene expression databases

BgeeP09036.
CleanExMM_SPINK3.
GenevestigatorP09036.

Family and domain databases

InterProIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamPF00050. Kazal_1. 1 hit.
[Graphical view]
PRINTSPR00290. KAZALINHBTR.
SMARTSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSPINK1. mouse.
NextBio299347.
PROP09036.
SOURCESearch...

Entry information

Entry nameISK3_MOUSE
AccessionPrimary (citable) accession number: P09036
Secondary accession number(s): Q5M9M3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot