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P09036

- ISK3_MOUSE

UniProt

P09036 - ISK3_MOUSE

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Protein

Serine protease inhibitor Kazal-type 3

Gene

Spink3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine protease inhibitor which exhibits anti-trypsin activity. Inhibits the uptake of calcium by spermatozoa.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei42 – 432Reactive bond

GO - Molecular functioni

  1. peptidase inhibitor activity Source: MGI
  2. serine-type endopeptidase inhibitor activity Source: MGI

GO - Biological processi

  1. negative regulation of calcium ion import Source: MGI
  2. negative regulation of nitric oxide mediated signal transduction Source: MGI
  3. negative regulation of peptidase activity Source: MGI
  4. negative regulation of peptidyl-tyrosine phosphorylation Source: MGI
  5. negative regulation of serine-type endopeptidase activity Source: MGI
  6. regulation of acrosome reaction Source: MGI
  7. regulation of store-operated calcium entry Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 3
Alternative name(s):
P12
Prostatic secretory glycoprotein
Gene namesi
Name:Spink3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:106202. Spink3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 8057Serine protease inhibitor Kazal-type 3PRO_0000016568Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 62PROSITE-ProRule annotation
Disulfide bondi40 ↔ 59PROSITE-ProRule annotation
Disulfide bondi48 ↔ 80PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP09036.
PRIDEiP09036.

Expressioni

Tissue specificityi

In the genital tract, expressed only in male accessory glands including seminal vesicle, coagulating gland and prostate.1 Publication

Developmental stagei

Not expressed during prepubertal stages. Expression coincides with maturation.1 Publication

Inductioni

By androgens in adult male sex accessory glands but expressed constitutively in pancreas.1 Publication

Gene expression databases

BgeeiP09036.
CleanExiMM_SPINK3.
GenevestigatoriP09036.

Interactioni

Protein-protein interaction databases

IntActiP09036. 1 interaction.
MINTiMINT-4098780.
STRINGi10090.ENSMUSP00000025381.

Structurei

3D structure databases

ProteinModelPortaliP09036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 8054Kazal-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG320895.
GeneTreeiENSGT00530000064228.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiP09036.
OrthoDBiEOG7BW0M2.
PhylomeDBiP09036.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
PRINTSiPR00290. KAZALINHBTR.
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09036-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVAVIFLLS ALALLSLAGN TFSAKVTGKE ASCHDAVAGC PRIYDPVCGT
60 70 80
DGITYANECV LCFENRKRIE PVLIRKGGPC
Length:80
Mass (Da):8,488
Last modified:November 1, 1988 - v1
Checksum:i4DC1F2EC4804CCA6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06342 mRNA. Translation: CAA29648.1.
AK007841 mRNA. Translation: BAB25298.1.
AK007985 mRNA. Translation: BAB25389.1.
BC086887 mRNA. Translation: AAH86887.1.
CCDSiCCDS37803.1.
PIRiS01498.
RefSeqiNP_033284.1. NM_009258.5.
UniGeneiMm.272.

Genome annotation databases

EnsembliENSMUST00000025381; ENSMUSP00000025381; ENSMUSG00000024503.
GeneIDi20730.
KEGGimmu:20730.
UCSCiuc008eul.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06342 mRNA. Translation: CAA29648.1 .
AK007841 mRNA. Translation: BAB25298.1 .
AK007985 mRNA. Translation: BAB25389.1 .
BC086887 mRNA. Translation: AAH86887.1 .
CCDSi CCDS37803.1.
PIRi S01498.
RefSeqi NP_033284.1. NM_009258.5.
UniGenei Mm.272.

3D structure databases

ProteinModelPortali P09036.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P09036. 1 interaction.
MINTi MINT-4098780.
STRINGi 10090.ENSMUSP00000025381.

Protein family/group databases

MEROPSi I01.011.

Proteomic databases

PaxDbi P09036.
PRIDEi P09036.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025381 ; ENSMUSP00000025381 ; ENSMUSG00000024503 .
GeneIDi 20730.
KEGGi mmu:20730.
UCSCi uc008eul.2. mouse.

Organism-specific databases

CTDi 20730.
MGIi MGI:106202. Spink3.

Phylogenomic databases

eggNOGi NOG320895.
GeneTreei ENSGT00530000064228.
HOGENOMi HOG000090244.
HOVERGENi HBG006182.
InParanoidi P09036.
OrthoDBi EOG7BW0M2.
PhylomeDBi P09036.

Miscellaneous databases

ChiTaRSi SPINK1. mouse.
NextBioi 299347.
PROi P09036.
SOURCEi Search...

Gene expression databases

Bgeei P09036.
CleanExi MM_SPINK3.
Genevestigatori P09036.

Family and domain databases

InterProi IPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view ]
Pfami PF00050. Kazal_1. 1 hit.
[Graphical view ]
PRINTSi PR00290. KAZALINHBTR.
SMARTi SM00280. KAZAL. 1 hit.
[Graphical view ]
PROSITEi PS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A secretory protease inhibitor requires androgens for its expression in male sex accessory tissues but is expressed constitutively in pancreas."
    Mills J.S., Needham M., Parker M.G.
    EMBO J. 6:3711-3717(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
    Strain: C57BL/6.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Pancreas.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "Purification and characterization of a trypsin inhibitor from mouse seminal vesicle secretion."
    Lai M.-L., Chen S.-W., Chen Y.-H.
    Arch. Biochem. Biophys. 290:265-271(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 43-47; 49-61 AND 63-72.
  5. "Developmental profile of a caltrin-like protease inhibitor, P12, in mouse seminal vesicle and characterization of its binding sites on sperm surface."
    Chen L.-Y., Lin Y.-H., Lai M.-L., Chen Y.-H.
    Biol. Reprod. 59:1498-1505(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiISK3_MOUSE
AccessioniPrimary (citable) accession number: P09036
Secondary accession number(s): Q5M9M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: October 1, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3