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P09034

- ASSY_RAT

UniProt

P09034 - ASSY_RAT

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Protein

Argininosuccinate synthase

Gene
Ass1, Ass
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Is indirectly involved in the control of blood pressure.1 Publication

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei87 – 871Citrulline By similarity
Binding sitei92 – 921Citrulline By similarity
Binding sitei119 – 1191Aspartate By similarity
Binding sitei123 – 1231Aspartate By similarity
Binding sitei123 – 1231Citrulline By similarity
Binding sitei124 – 1241Aspartate By similarity
Binding sitei127 – 1271Citrulline By similarity
Binding sitei180 – 1801Citrulline By similarity
Binding sitei189 – 1891Citrulline By similarity
Binding sitei270 – 2701Citrulline By similarity
Binding sitei282 – 2821Citrulline By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATP By similarity
Nucleotide bindingi115 – 1239ATP By similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: RGD
  2. ATP binding Source: UniProtKB-KW
  3. toxic substance binding Source: RGD

GO - Biological processi

  1. acute-phase response Source: RGD
  2. aging Source: RGD
  3. arginine biosynthetic process Source: RGD
  4. argininosuccinate metabolic process Source: RGD
  5. cellular response to amine stimulus Source: RGD
  6. cellular response to amino acid stimulus Source: RGD
  7. cellular response to ammonium ion Source: RGD
  8. cellular response to cAMP Source: RGD
  9. cellular response to dexamethasone stimulus Source: RGD
  10. cellular response to glucagon stimulus Source: RGD
  11. cellular response to interferon-gamma Source: RGD
  12. cellular response to lipopolysaccharide Source: RGD
  13. cellular response to oleic acid Source: RGD
  14. cellular response to tumor necrosis factor Source: RGD
  15. diaphragm development Source: RGD
  16. kidney development Source: RGD
  17. liver development Source: RGD
  18. midgut development Source: RGD
  19. response to acid Source: RGD
  20. response to amine Source: RGD
  21. response to amino acid Source: RGD
  22. response to drug Source: RGD
  23. response to estradiol Source: RGD
  24. response to fatty acid Source: RGD
  25. response to glucocorticoid Source: RGD
  26. response to growth hormone Source: RGD
  27. response to lipopolysaccharide Source: RGD
  28. response to mycotoxin Source: RGD
  29. response to nutrient Source: RGD
  30. response to peptide hormone Source: RGD
  31. response to steroid hormone Source: RGD
  32. response to zinc ion Source: RGD
  33. urea cycle Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_216480. Urea cycle.
SABIO-RKP09034.
UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:Ass1
Synonyms:Ass
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi2163. Ass1.

Subcellular locationi

GO - Cellular componenti

  1. cell body fiber Source: RGD
  2. cytoplasm Source: RGD
  3. endoplasmic reticulum Source: RGD
  4. lysosome Source: RGD
  5. mitochondrial outer membrane Source: RGD
  6. neuronal cell body Source: RGD
  7. neuron projection Source: RGD
  8. nucleus Source: RGD
  9. perikaryon Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Argininosuccinate synthaseUniRule annotationPRO_0000148556Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei180 – 1801Phosphoserine By similarity
Modified residuei219 – 2191Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP09034.
PRIDEiP09034.

PTM databases

PhosphoSiteiP09034.

Expressioni

Gene expression databases

GenevestigatoriP09034.

Interactioni

Subunit structurei

Homotetramer. Interacts with NMRAL1 By similarity.UniRule annotation

Protein-protein interaction databases

MINTiMINT-4563555.
STRINGi10116.ENSRNOP00000012075.

Structurei

3D structure databases

ProteinModelPortaliP09034.
SMRiP09034. Positions 4-407.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
GeneTreeiENSGT00390000004524.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiP09034.
KOiK01940.
OMAiQGDYEPA.
OrthoDBiEOG7PVWPB.
PhylomeDBiP09034.
TreeFamiTF300736.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09034-1 [UniParc]FASTAAdd to Basket

« Hide

MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK    50
ALKLGAKKVF IEDVSKEFVE EFIWPAVQSS ALYEDRYLLG TSLARPCIAR 100
KQVEIAQREG AKYVSHGATG KGNDQVRFEL TCYSLAPQIK VIAPWRMPEF 150
YNRFKGRNDL MEYAKQHGIP IPVTPKSPWS MDENLMHISY EAGILENPKN 200
QAPPGLYTKT QDPAKAPNTP DVLEIEFKKG VPVKVTNVKD GTTHSTSLDL 250
FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF 300
TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIDK SQERVEGKVQ 350
VSVFKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPIDATG FININSLRLK 400
EYHRLQSKVT AK 412
Length:412
Mass (Da):46,496
Last modified:November 1, 1988 - v1
Checksum:iCCA80906F5A3E93D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12459 mRNA. Translation: CAA30999.1.
M36708 mRNA. Translation: AAA40771.1.
BC063146 mRNA. Translation: AAH63146.1.
PIRiS01440. AJRTRS.
RefSeqiNP_037289.1. NM_013157.3.
XP_006233973.1. XM_006233911.1.
UniGeneiRn.5078.

Genome annotation databases

EnsembliENSRNOT00000012075; ENSRNOP00000012075; ENSRNOG00000008837.
GeneIDi25698.
KEGGirno:25698.
UCSCiRGD:2163. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12459 mRNA. Translation: CAA30999.1 .
M36708 mRNA. Translation: AAA40771.1 .
BC063146 mRNA. Translation: AAH63146.1 .
PIRi S01440. AJRTRS.
RefSeqi NP_037289.1. NM_013157.3.
XP_006233973.1. XM_006233911.1.
UniGenei Rn.5078.

3D structure databases

ProteinModelPortali P09034.
SMRi P09034. Positions 4-407.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4563555.
STRINGi 10116.ENSRNOP00000012075.

PTM databases

PhosphoSitei P09034.

Proteomic databases

PaxDbi P09034.
PRIDEi P09034.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000012075 ; ENSRNOP00000012075 ; ENSRNOG00000008837 .
GeneIDi 25698.
KEGGi rno:25698.
UCSCi RGD:2163. rat.

Organism-specific databases

CTDi 445.
RGDi 2163. Ass1.

Phylogenomic databases

eggNOGi COG0137.
GeneTreei ENSGT00390000004524.
HOGENOMi HOG000230093.
HOVERGENi HBG001717.
InParanoidi P09034.
KOi K01940.
OMAi QGDYEPA.
OrthoDBi EOG7PVWPB.
PhylomeDBi P09034.
TreeFami TF300736.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .
UPA00158 ; UER00272 .
Reactomei REACT_216480. Urea cycle.
SABIO-RK P09034.

Miscellaneous databases

NextBioi 607719.
PROi P09034.

Gene expression databases

Genevestigatori P09034.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the cDNA encoding the rat argininosuccinate synthetase."
    Surh L.C., Morris S.M., O'Brien W.E., Beaudet A.L.
    Nucleic Acids Res. 16:9352-9352(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. "Argininosuccinate synthetase is a functional target for a snake venom anti-hypertensive peptide: role in arginine and nitric oxide production."
    Guerreiro J.R., Lameu C., Oliveira E.F., Klitzke C.F., Melo R.L., Linares E., Augusto O., Fox J.W., Lebrun I., Serrano S.M., Camargo A.C.
    J. Biol. Chem. 284:20022-20033(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOASSAY.

Entry informationi

Entry nameiASSY_RAT
AccessioniPrimary (citable) accession number: P09034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: September 3, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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