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P09034

- ASSY_RAT

UniProt

P09034 - ASSY_RAT

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Protein

Argininosuccinate synthase

Gene

Ass1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Is indirectly involved in the control of blood pressure.1 Publication

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei87 – 871CitrullineBy similarity
Binding sitei92 – 921CitrullineBy similarity
Binding sitei119 – 1191AspartateBy similarity
Binding sitei123 – 1231AspartateBy similarity
Binding sitei123 – 1231CitrullineBy similarity
Binding sitei124 – 1241AspartateBy similarity
Binding sitei127 – 1271CitrullineBy similarity
Binding sitei180 – 1801CitrullineBy similarity
Binding sitei189 – 1891CitrullineBy similarity
Binding sitei270 – 2701CitrullineBy similarity
Binding sitei282 – 2821CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPBy similarity
Nucleotide bindingi115 – 1239ATPBy similarity

GO - Molecular functioni

  1. amino acid binding Source: Ensembl
  2. argininosuccinate synthase activity Source: RGD
  3. ATP binding Source: UniProtKB-KW
  4. poly(A) RNA binding Source: Ensembl
  5. toxic substance binding Source: RGD

GO - Biological processi

  1. acute-phase response Source: RGD
  2. aging Source: RGD
  3. arginine biosynthetic process Source: RGD
  4. argininosuccinate metabolic process Source: RGD
  5. aspartate metabolic process Source: Ensembl
  6. cellular response to amine stimulus Source: RGD
  7. cellular response to amino acid stimulus Source: RGD
  8. cellular response to ammonium ion Source: RGD
  9. cellular response to cAMP Source: RGD
  10. cellular response to dexamethasone stimulus Source: RGD
  11. cellular response to glucagon stimulus Source: RGD
  12. cellular response to interferon-gamma Source: RGD
  13. cellular response to laminar fluid shear stress Source: Ensembl
  14. cellular response to lipopolysaccharide Source: RGD
  15. cellular response to oleic acid Source: RGD
  16. cellular response to tumor necrosis factor Source: RGD
  17. citrulline metabolic process Source: Ensembl
  18. diaphragm development Source: RGD
  19. kidney development Source: RGD
  20. liver development Source: RGD
  21. midgut development Source: RGD
  22. negative regulation of leukocyte cell-cell adhesion Source: Ensembl
  23. positive regulation of nitric oxide biosynthetic process Source: Ensembl
  24. response to amine Source: RGD
  25. response to amino acid Source: RGD
  26. response to drug Source: RGD
  27. response to estradiol Source: RGD
  28. response to fatty acid Source: RGD
  29. response to glucocorticoid Source: RGD
  30. response to growth hormone Source: RGD
  31. response to lipopolysaccharide Source: RGD
  32. response to mycotoxin Source: RGD
  33. response to nutrient Source: RGD
  34. response to peptide hormone Source: RGD
  35. response to steroid hormone Source: RGD
  36. response to toxic substance Source: RGD
  37. response to zinc ion Source: RGD
  38. urea cycle Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_216480. Urea cycle.
SABIO-RKP09034.
UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:Ass1
Synonyms:Ass
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi2163. Ass1.

Subcellular locationi

GO - Cellular componenti

  1. cell body fiber Source: RGD
  2. cytoplasm Source: RGD
  3. endoplasmic reticulum Source: RGD
  4. extracellular vesicular exosome Source: Ensembl
  5. lysosome Source: RGD
  6. mitochondrial outer membrane Source: RGD
  7. neuronal cell body Source: RGD
  8. neuron projection Source: RGD
  9. nucleus Source: RGD
  10. perikaryon Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Argininosuccinate synthasePRO_0000148556Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei180 – 1801PhosphoserineBy similarity
Modified residuei219 – 2191PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP09034.
PRIDEiP09034.

PTM databases

PhosphoSiteiP09034.

Expressioni

Gene expression databases

GenevestigatoriP09034.

Interactioni

Subunit structurei

Homotetramer. Interacts with NMRAL1 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4563555.
STRINGi10116.ENSRNOP00000012075.

Structurei

3D structure databases

ProteinModelPortaliP09034.
SMRiP09034. Positions 4-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
GeneTreeiENSGT00390000004524.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiP09034.
KOiK01940.
OMAiQGDYEPA.
OrthoDBiEOG7PVWPB.
PhylomeDBiP09034.
TreeFamiTF300736.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09034 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK
60 70 80 90 100
ALKLGAKKVF IEDVSKEFVE EFIWPAVQSS ALYEDRYLLG TSLARPCIAR
110 120 130 140 150
KQVEIAQREG AKYVSHGATG KGNDQVRFEL TCYSLAPQIK VIAPWRMPEF
160 170 180 190 200
YNRFKGRNDL MEYAKQHGIP IPVTPKSPWS MDENLMHISY EAGILENPKN
210 220 230 240 250
QAPPGLYTKT QDPAKAPNTP DVLEIEFKKG VPVKVTNVKD GTTHSTSLDL
260 270 280 290 300
FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF
310 320 330 340 350
TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIDK SQERVEGKVQ
360 370 380 390 400
VSVFKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPIDATG FININSLRLK
410
EYHRLQSKVT AK
Length:412
Mass (Da):46,496
Last modified:November 1, 1988 - v1
Checksum:iCCA80906F5A3E93D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12459 mRNA. Translation: CAA30999.1.
M36708 mRNA. Translation: AAA40771.1.
BC063146 mRNA. Translation: AAH63146.1.
PIRiS01440. AJRTRS.
RefSeqiNP_037289.1. NM_013157.3.
XP_006233973.1. XM_006233911.2.
UniGeneiRn.5078.

Genome annotation databases

EnsembliENSRNOT00000012075; ENSRNOP00000012075; ENSRNOG00000008837.
GeneIDi25698.
KEGGirno:25698.
UCSCiRGD:2163. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12459 mRNA. Translation: CAA30999.1 .
M36708 mRNA. Translation: AAA40771.1 .
BC063146 mRNA. Translation: AAH63146.1 .
PIRi S01440. AJRTRS.
RefSeqi NP_037289.1. NM_013157.3.
XP_006233973.1. XM_006233911.2.
UniGenei Rn.5078.

3D structure databases

ProteinModelPortali P09034.
SMRi P09034. Positions 4-407.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4563555.
STRINGi 10116.ENSRNOP00000012075.

PTM databases

PhosphoSitei P09034.

Proteomic databases

PaxDbi P09034.
PRIDEi P09034.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000012075 ; ENSRNOP00000012075 ; ENSRNOG00000008837 .
GeneIDi 25698.
KEGGi rno:25698.
UCSCi RGD:2163. rat.

Organism-specific databases

CTDi 445.
RGDi 2163. Ass1.

Phylogenomic databases

eggNOGi COG0137.
GeneTreei ENSGT00390000004524.
HOGENOMi HOG000230093.
HOVERGENi HBG001717.
InParanoidi P09034.
KOi K01940.
OMAi QGDYEPA.
OrthoDBi EOG7PVWPB.
PhylomeDBi P09034.
TreeFami TF300736.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .
UPA00158 ; UER00272 .
Reactomei REACT_216480. Urea cycle.
SABIO-RK P09034.

Miscellaneous databases

NextBioi 607719.
PROi P09034.

Gene expression databases

Genevestigatori P09034.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the cDNA encoding the rat argininosuccinate synthetase."
    Surh L.C., Morris S.M., O'Brien W.E., Beaudet A.L.
    Nucleic Acids Res. 16:9352-9352(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. "Argininosuccinate synthetase is a functional target for a snake venom anti-hypertensive peptide: role in arginine and nitric oxide production."
    Guerreiro J.R., Lameu C., Oliveira E.F., Klitzke C.F., Melo R.L., Linares E., Augusto O., Fox J.W., Lebrun I., Serrano S.M., Camargo A.C.
    J. Biol. Chem. 284:20022-20033(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOASSAY.

Entry informationi

Entry nameiASSY_RAT
AccessioniPrimary (citable) accession number: P09034
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3