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P09034

- ASSY_RAT

UniProt

P09034 - ASSY_RAT

Protein

Argininosuccinate synthase

Gene

Ass1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    Is indirectly involved in the control of blood pressure.1 Publication

    Catalytic activityi

    ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei87 – 871CitrullineBy similarity
    Binding sitei92 – 921CitrullineBy similarity
    Binding sitei119 – 1191AspartateBy similarity
    Binding sitei123 – 1231AspartateBy similarity
    Binding sitei123 – 1231CitrullineBy similarity
    Binding sitei124 – 1241AspartateBy similarity
    Binding sitei127 – 1271CitrullineBy similarity
    Binding sitei180 – 1801CitrullineBy similarity
    Binding sitei189 – 1891CitrullineBy similarity
    Binding sitei270 – 2701CitrullineBy similarity
    Binding sitei282 – 2821CitrullineBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPBy similarity
    Nucleotide bindingi115 – 1239ATPBy similarity

    GO - Molecular functioni

    1. argininosuccinate synthase activity Source: RGD
    2. ATP binding Source: UniProtKB-KW
    3. toxic substance binding Source: RGD

    GO - Biological processi

    1. acute-phase response Source: RGD
    2. aging Source: RGD
    3. arginine biosynthetic process Source: RGD
    4. argininosuccinate metabolic process Source: RGD
    5. cellular response to amine stimulus Source: RGD
    6. cellular response to amino acid stimulus Source: RGD
    7. cellular response to ammonium ion Source: RGD
    8. cellular response to cAMP Source: RGD
    9. cellular response to dexamethasone stimulus Source: RGD
    10. cellular response to glucagon stimulus Source: RGD
    11. cellular response to interferon-gamma Source: RGD
    12. cellular response to lipopolysaccharide Source: RGD
    13. cellular response to oleic acid Source: RGD
    14. cellular response to tumor necrosis factor Source: RGD
    15. diaphragm development Source: RGD
    16. kidney development Source: RGD
    17. liver development Source: RGD
    18. midgut development Source: RGD
    19. response to acid chemical Source: RGD
    20. response to amine Source: RGD
    21. response to amino acid Source: RGD
    22. response to drug Source: RGD
    23. response to estradiol Source: RGD
    24. response to fatty acid Source: RGD
    25. response to glucocorticoid Source: RGD
    26. response to growth hormone Source: RGD
    27. response to lipopolysaccharide Source: RGD
    28. response to mycotoxin Source: RGD
    29. response to nutrient Source: RGD
    30. response to peptide hormone Source: RGD
    31. response to steroid hormone Source: RGD
    32. response to zinc ion Source: RGD
    33. urea cycle Source: RGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_216480. Urea cycle.
    SABIO-RKP09034.
    UniPathwayiUPA00068; UER00113.
    UPA00158; UER00272.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Argininosuccinate synthase (EC:6.3.4.5)
    Alternative name(s):
    Citrulline--aspartate ligase
    Gene namesi
    Name:Ass1
    Synonyms:Ass
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi2163. Ass1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell body fiber Source: RGD
    2. cytoplasm Source: RGD
    3. endoplasmic reticulum Source: RGD
    4. lysosome Source: RGD
    5. mitochondrial outer membrane Source: RGD
    6. neuronal cell body Source: RGD
    7. neuron projection Source: RGD
    8. nucleus Source: RGD
    9. perikaryon Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 412412Argininosuccinate synthasePRO_0000148556Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei180 – 1801PhosphoserineBy similarity
    Modified residuei219 – 2191PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP09034.
    PRIDEiP09034.

    PTM databases

    PhosphoSiteiP09034.

    Expressioni

    Gene expression databases

    GenevestigatoriP09034.

    Interactioni

    Subunit structurei

    Homotetramer. Interacts with NMRAL1 By similarity.By similarity

    Protein-protein interaction databases

    MINTiMINT-4563555.
    STRINGi10116.ENSRNOP00000012075.

    Structurei

    3D structure databases

    ProteinModelPortaliP09034.
    SMRiP09034. Positions 4-407.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0137.
    GeneTreeiENSGT00390000004524.
    HOGENOMiHOG000230093.
    HOVERGENiHBG001717.
    InParanoidiP09034.
    KOiK01940.
    OMAiQGDYEPA.
    OrthoDBiEOG7PVWPB.
    PhylomeDBiP09034.
    TreeFamiTF300736.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPiMF_00005. Arg_succ_synth_type1.
    InterProiIPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00764. Arginosuc_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00032. argG. 1 hit.
    PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09034-1 [UniParc]FASTAAdd to Basket

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    MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK    50
    ALKLGAKKVF IEDVSKEFVE EFIWPAVQSS ALYEDRYLLG TSLARPCIAR 100
    KQVEIAQREG AKYVSHGATG KGNDQVRFEL TCYSLAPQIK VIAPWRMPEF 150
    YNRFKGRNDL MEYAKQHGIP IPVTPKSPWS MDENLMHISY EAGILENPKN 200
    QAPPGLYTKT QDPAKAPNTP DVLEIEFKKG VPVKVTNVKD GTTHSTSLDL 250
    FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF 300
    TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIDK SQERVEGKVQ 350
    VSVFKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPIDATG FININSLRLK 400
    EYHRLQSKVT AK 412
    Length:412
    Mass (Da):46,496
    Last modified:November 1, 1988 - v1
    Checksum:iCCA80906F5A3E93D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12459 mRNA. Translation: CAA30999.1.
    M36708 mRNA. Translation: AAA40771.1.
    BC063146 mRNA. Translation: AAH63146.1.
    PIRiS01440. AJRTRS.
    RefSeqiNP_037289.1. NM_013157.3.
    XP_006233973.1. XM_006233911.1.
    UniGeneiRn.5078.

    Genome annotation databases

    EnsembliENSRNOT00000012075; ENSRNOP00000012075; ENSRNOG00000008837.
    GeneIDi25698.
    KEGGirno:25698.
    UCSCiRGD:2163. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12459 mRNA. Translation: CAA30999.1 .
    M36708 mRNA. Translation: AAA40771.1 .
    BC063146 mRNA. Translation: AAH63146.1 .
    PIRi S01440. AJRTRS.
    RefSeqi NP_037289.1. NM_013157.3.
    XP_006233973.1. XM_006233911.1.
    UniGenei Rn.5078.

    3D structure databases

    ProteinModelPortali P09034.
    SMRi P09034. Positions 4-407.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4563555.
    STRINGi 10116.ENSRNOP00000012075.

    PTM databases

    PhosphoSitei P09034.

    Proteomic databases

    PaxDbi P09034.
    PRIDEi P09034.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000012075 ; ENSRNOP00000012075 ; ENSRNOG00000008837 .
    GeneIDi 25698.
    KEGGi rno:25698.
    UCSCi RGD:2163. rat.

    Organism-specific databases

    CTDi 445.
    RGDi 2163. Ass1.

    Phylogenomic databases

    eggNOGi COG0137.
    GeneTreei ENSGT00390000004524.
    HOGENOMi HOG000230093.
    HOVERGENi HBG001717.
    InParanoidi P09034.
    KOi K01940.
    OMAi QGDYEPA.
    OrthoDBi EOG7PVWPB.
    PhylomeDBi P09034.
    TreeFami TF300736.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00113 .
    UPA00158 ; UER00272 .
    Reactomei REACT_216480. Urea cycle.
    SABIO-RK P09034.

    Miscellaneous databases

    NextBioi 607719.
    PROi P09034.

    Gene expression databases

    Genevestigatori P09034.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPi MF_00005. Arg_succ_synth_type1.
    InterProi IPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00764. Arginosuc_synth. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00032. argG. 1 hit.
    PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the cDNA encoding the rat argininosuccinate synthetase."
      Surh L.C., Morris S.M., O'Brien W.E., Beaudet A.L.
      Nucleic Acids Res. 16:9352-9352(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    3. "Argininosuccinate synthetase is a functional target for a snake venom anti-hypertensive peptide: role in arginine and nitric oxide production."
      Guerreiro J.R., Lameu C., Oliveira E.F., Klitzke C.F., Melo R.L., Linares E., Augusto O., Fox J.W., Lebrun I., Serrano S.M., Camargo A.C.
      J. Biol. Chem. 284:20022-20033(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOASSAY.

    Entry informationi

    Entry nameiASSY_RAT
    AccessioniPrimary (citable) accession number: P09034
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3