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Protein

Translation initiation factor eIF-2B subunit gamma

Gene

GCD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as essential component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD1 stabilizes the interaction between eIF-2 and GCD6 and stimulates the catalytic activity in vitro.2 Publications

Miscellaneous

Present with 9530 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • translation initiation factor activity Source: SGD

GO - Biological processi

  • negative regulation of cellular response to amino acid starvation Source: SGD
  • regulation of molecular function Source: GOC
  • regulation of translational initiation Source: SGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33751-MONOMER
ReactomeiR-SCE-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor eIF-2B subunit gamma
Alternative name(s):
GCD complex subunit GCD1
Guanine nucleotide exchange factor subunit GCD1
eIF-2B GDP-GTP exchange factor subunit gamma
Gene namesi
Name:GCD1
Synonyms:TIF223, TRA3
Ordered Locus Names:YOR260W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR260W
SGDiS000005786 GCD1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001560831 – 578Translation initiation factor eIF-2B subunit gammaAdd BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei296PhosphoserineCombined sources1
Modified residuei300PhosphoserineCombined sources1
Modified residuei306PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP09032
PaxDbiP09032
PRIDEiP09032

PTM databases

iPTMnetiP09032

Interactioni

Subunit structurei

Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A catalytic subcomplex comprising GCD1 and GCD6 interacts with both, phosphorylated and non-phosphorylated eIF-2 and has exchange activity in vitro.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34650, 371 interactors
DIPiDIP-1327N
IntActiP09032, 26 interactors
MINTiP09032
STRINGi4932.YOR260W

Structurei

3D structure databases

ProteinModelPortaliP09032
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000047486
HOGENOMiHOG000248845
InParanoidiP09032
KOiK03241
OMAiEFLEMPR
OrthoDBiEOG092C1UCY

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR029044 Nucleotide-diphossugar_trans
IPR011004 Trimer_LpxA-like_sf
SUPFAMiSSF51161 SSF51161, 1 hit
SSF53448 SSF53448, 3 hits

Sequencei

Sequence statusi: Complete.

P09032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIQAFVFCG KGSNLAPFTQ PDFPFQTQNK DSTAATSGDK LNELVNSALD
60 70 80 90 100
STVINEFMQH STRLPKALLP IGNRPMIEYV LDWCDQADFK EISVVAPVDE
110 120 130 140 150
IELIESGLTS FLSLRKQQFE LIYKALSNSN HSHHLQDPKK INFIPSKANS
160 170 180 190 200
TGESLQKELL PRINGDFVIL PCDFVTDIPP QVLVDQFRNR DDNNLAMTIY
210 220 230 240 250
YKNSLDSSID KKQQQKQKQQ QFFTVYSENE DSERQPILLD VYSQRDVTKT
260 270 280 290 300
KYLQIRSHLL WNYPNLTVST KLLNSFIYFC SFELCQLLKL GPQSMSRQAS
310 320 330 340 350
FKDPFTGNQQ QQNPPTTDDD EDRNHDDDDD YKPSATSIQP TYFKKKNDLI
360 370 380 390 400
LDPINCNKSL SKVFRDLSRR SWQHSKPREP IGIFILPNET LFIRANNLNA
410 420 430 440 450
YMDANRFVLK IKSQTMFTKN IQIQSAAIGA DAIVDPKCQI SAHSNVKMSV
460 470 480 490 500
LGTQANIGSR CRVAGSLLFP GVHLGDEVIL ENCIIGPMAK IGSKCKLSNC
510 520 530 540 550
YIEGHYVVEP KNNFKGETLA NVYLDEDEED ELIYDDSVIA GESEIAEETD
560 570
SDDRSDEDSD DSEYTDEYEY EDDGLFER
Length:578
Mass (Da):65,700
Last modified:November 1, 1997 - v3
Checksum:iEC25BAE00F4D1E94
GO

Sequence cautioni

The sequence CAA30693 differs from that shown. Reason: Frameshift at position 503.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti217 – 240QKQQQ…PILLD → AKNNSNFSLFIQKTKTQRGS QYFWN in CAA30693 (PubMed:3050897).CuratedAdd BLAST24
Sequence conflicti491 – 492IG → SV in CAA30693 (PubMed:3050897).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07846 Genomic DNA Translation: CAA30693.1 Frameshift.
Z75168 Genomic DNA Translation: CAA99482.1
BK006948 Genomic DNA Translation: DAA11027.1
PIRiS67157 BVBYD1
RefSeqiNP_014903.1, NM_001183679.1

Genome annotation databases

EnsemblFungiiYOR260W; YOR260W; YOR260W
GeneIDi854434
KEGGisce:YOR260W

Similar proteinsi

Entry informationi

Entry nameiEI2BG_YEAST
AccessioniPrimary (citable) accession number: P09032
Secondary accession number(s): D6W2W1, Q08721
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health