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Protein

Homeobox protein Hox-A1

Gene

Hoxa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments.
The homeobox domain presumably directs sequence-specific DNA binding. The N-terminal portion of ERA-1-993 may be involved in interactions with one or more other regulatory proteins. Such an interaction could regulate either the DNA-binding activity or the transcriptional regulatory activity of ERA-1-993.
The homeoboxless ERA-1-399 protein could act as a competitive inhibitor of the ERA-1-993 protein by competing for interaction with regulatory protein(s) while being unable to bind to DNA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi225 – 284HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • abducens nerve formation Source: MGI
  • anatomical structure formation involved in morphogenesis Source: MGI
  • anatomical structure morphogenesis Source: MGI
  • anterior/posterior pattern specification Source: MGI
  • artery development Source: MGI
  • artery morphogenesis Source: MGI
  • cellular response to ethanol Source: MGI
  • cellular response to retinoic acid Source: MGI
  • central nervous system neuron differentiation Source: MGI
  • cochlea development Source: MGI
  • cochlea morphogenesis Source: MGI
  • cognition Source: MGI
  • embryonic neurocranium morphogenesis Source: MGI
  • facial nerve structural organization Source: MGI
  • facial nucleus development Source: MGI
  • hindbrain development Source: MGI
  • inner ear development Source: MGI
  • inner ear morphogenesis Source: MGI
  • motor neuron axon guidance Source: MGI
  • nervous system development Source: MGI
  • neuromuscular process Source: MGI
  • optokinetic behavior Source: MGI
  • outer ear morphogenesis Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of behavior Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • rhombomere 3 development Source: MGI
  • rhombomere 4 development Source: MGI
  • rhombomere 5 development Source: MGI
  • semicircular canal formation Source: MGI
  • sensory perception of sound Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Hox-A1
Alternative name(s):
Early retinoic acid 1
Homeobox protein Hox-1.6
Homeoboxless protein ERA-1-399
Homeotic protein ERA-1-993
Gene namesi
Name:Hoxa1
Synonyms:Era-1, Hox-1.6, Hoxa-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96170. Hoxa1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002000311 – 331Homeobox protein Hox-A1Add BLAST331

Proteomic databases

PaxDbiP09022.
PRIDEiP09022.

Expressioni

Developmental stagei

Expressed along the entire length of the primitive streak. In early neurogenesis it is expressed in lateral and paraxial mesoderm, endoderm and superficial ectoderm or in the neural tube. From late neurogenesis to mid-embryogenesis, it presents similar spatial domains in the lateral mesoderm, endoderm and superficial ectoderm but is not detectable in any part of the hindbrain. At day 8-8.5 post-coitum found in the embryonic mesoderm, anterior to the first somite, up to the cephalic region at the level of the foregut and developing heart. At day 9 post-coitum found in an anterior part of the developing foregut and is restricted to an epithelial cell type. Expression in the gut is found at the level of the second to third branchial bars.1 Publication

Inductioni

By retinoic acid.

Gene expression databases

CleanExiMM_HOXA1.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-59869N.
IntActiP09022. 61 interactors.
STRINGi10090.ENSMUSP00000000964.

Structurei

3D structure databases

ProteinModelPortaliP09022.
SMRiP09022.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi200 – 205Antp-type hexapeptide6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi60 – 70Poly-HisAdd BLAST11
Compositional biasi138 – 142Poly-His5

Sequence similaritiesi

Belongs to the Antp homeobox family. Labial subfamily.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0489. Eukaryota.
ENOG410ZTBY. LUCA.
HOGENOMiHOG000171545.
HOVERGENiHBG006089.
InParanoidiP09022.

Family and domain databases

CDDicd00086. homeodomain. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PRINTSiPR00024. HOMEOBOX.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform ERA-1-993 (identifier: P09022-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSFLEYPIL GSGDSGTCSA RAYPSDHGIT TFQSCAVSAN SCGGDDRFLV
60 70 80 90 100
GRGVQISSPH HHHHHHHHHH PQTATYQTSG NLGISYSHSS CGPSYGAQNF
110 120 130 140 150
SAPYGPYGLN QEADVSGGYP PCAPAVYSGN LSTPMVQHHH HHQGYAGGTV
160 170 180 190 200
GSPQYIHHSY GQEQQTLALA TYNNSLSPLH ASHQEACRSP ASETSSPAQT
210 220 230 240 250
FDWMKVKRNP PKTGKVGEYG YVGQPNAVRT NFTTKQLTEL EKEFHFNKYL
260 270 280 290 300
TRARRVEIAA SLQLNETQVK IWFQNRRMKQ KKREKEGLLP ISPATPPGSD
310 320 330
EKTEESSEKS SPSPSAPSPA SSTSDTLTTS H
Length:331
Mass (Da):36,037
Last modified:April 1, 1990 - v2
Checksum:iF73E4FFB1C6F15B2
GO
Isoform ERA-1-399 (identifier: P09022-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-133: VSGGYPPCAPAVYSGNLST → PPRSLSFPCFRDVFSSADL
     134-331: Missing.

Show »
Length:133
Mass (Da):14,290
Checksum:iF3A12654DA61A299
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002380115 – 133VSGGY…GNLST → PPRSLSFPCFRDVFSSADL in isoform ERA-1-399. CuratedAdd BLAST19
Alternative sequenceiVSP_002381134 – 331Missing in isoform ERA-1-399. CuratedAdd BLAST198

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22115 mRNA. Translation: AAA37559.1.
M22115 mRNA. Translation: AAA37558.1.
X06023 Genomic DNA. Translation: CAA29426.1.
X06024 Genomic DNA. Translation: CAA29427.1.
M20214 Genomic DNA. No translation available.
M20216, M20215 Genomic DNA. Translation: AAA37839.1.
PIRiA27155.
A30242.
B30242.
UniGeneiMm.197.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHXA1_MOUSE
AccessioniPrimary (citable) accession number: P09022
Secondary accession number(s): P15463
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: April 1, 1990
Last modified: September 27, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Vertebrate homeotic Hox proteins
    Nomenclature of vertebrate homeotic Hox proteins and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families