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P09022 (HXA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein Hox-A1
Alternative name(s):
Early retinoic acid 1
Homeobox protein Hox-1.6
Homeoboxless protein ERA-1-399
Homeotic protein ERA-1-993
Gene names
Name:Hoxa1
Synonyms:Era-1, Hox-1.6, Hoxa-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments.

The homeobox domain presumably directs sequence-specific DNA binding. The N-terminal portion of ERA-1-993 may be involved in interactions with one or more other regulatory proteins. Such an interaction could regulate either the DNA-binding activity or the transcriptional regulatory activity of ERA-1-993.

The homeoboxless ERA-1-399 protein could act as a competitive inhibitor of the ERA-1-993 protein by competing for interaction with regulatory protein(s) while being unable to bind to DNA.

Subcellular location

Nucleus.

Developmental stage

Expressed along the entire length of the primitive streak. In early neurogenesis it is expressed in lateral and paraxial mesoderm, endoderm and superficial ectoderm or in the neural tube. From late neurogenesis to mid-embryogenesis, it presents similar spatial domains in the lateral mesoderm, endoderm and superficial ectoderm but is not detectable in any part of the hindbrain. At day 8-8.5 post-coitum found in the embryonic mesoderm, anterior to the first somite, up to the cephalic region at the level of the foregut and developing heart. At day 9 post-coitum found in an anterior part of the developing foregut and is restricted to an epithelial cell type. Expression in the gut is found at the level of the second to third branchial bars. Ref.3

Induction

By retinoic acid.

Sequence similarities

Belongs to the Antp homeobox family. Labial subfamily.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processabducens nerve formation

Inferred from electronic annotation. Source: Ensembl

anatomical structure formation involved in morphogenesis

Inferred from genetic interaction PubMed 10529420. Source: MGI

anatomical structure morphogenesis

Inferred from genetic interaction PubMed 10529420. Source: MGI

anterior/posterior pattern specification

Inferred from mutant phenotype PubMed 15367676. Source: MGI

artery morphogenesis

Inferred from electronic annotation. Source: Ensembl

central nervous system neuron differentiation

Inferred from mutant phenotype PubMed 9806922. Source: MGI

cochlea morphogenesis

Inferred from electronic annotation. Source: Ensembl

cognition

Inferred from electronic annotation. Source: Ensembl

embryonic neurocranium morphogenesis

Inferred from electronic annotation. Source: Ensembl

facial nerve structural organization

Inferred from genetic interaction PubMed 9806922. Source: MGI

facial nucleus development

Inferred from mutant phenotype PubMed 8102800. Source: MGI

hindbrain development

Inferred from mutant phenotype PubMed 15367676. Source: MGI

inner ear morphogenesis

Inferred from mutant phenotype PubMed 15367676. Source: MGI

motor neuron axon guidance

Inferred from genetic interaction PubMed 9806922. Source: MGI

nervous system development

Inferred from mutant phenotype PubMed 15367676. Source: MGI

neuromuscular process

Inferred from electronic annotation. Source: Ensembl

optokinetic behavior

Inferred from electronic annotation. Source: Ensembl

outer ear morphogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18787068. Source: MGI

regulation of behavior

Inferred from electronic annotation. Source: Ensembl

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15465489. Source: MGI

rhombomere 3 development

Inferred from mutant phenotype PubMed 9806922. Source: MGI

rhombomere 4 development

Inferred from mutant phenotype PubMed 8102800PubMed 9806922. Source: MGI

rhombomere 5 development

Inferred from mutant phenotype PubMed 8102800PubMed 9806922. Source: MGI

semicircular canal formation

Inferred from electronic annotation. Source: Ensembl

sensory perception of sound

Inferred from electronic annotation. Source: Ensembl

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ABHD17AQ96GS63EBI-3957603,EBI-2870273From a different organism.
ADAMTSL4Q6UY143EBI-3957603,EBI-742002From a different organism.
C1orf94Q6P1W53EBI-3957603,EBI-946029From a different organism.
EFCAB4BQ9BSW23EBI-3957603,EBI-739773From a different organism.
EFEMP2O959673EBI-3957603,EBI-743414From a different organism.
FAM154AQ8IYX73EBI-3957603,EBI-3957636From a different organism.
FHL5Q5TD973EBI-3957603,EBI-750641From a different organism.
GRNP287992EBI-3957603,EBI-747754From a different organism.
HOXA1P496394EBI-3957603,EBI-740785From a different organism.
HOXD3P312493EBI-3957603,EBI-3957655From a different organism.
IKZF2Q9UKS73EBI-3957603,EBI-3893057From a different organism.
KRT81Q145333EBI-3957603,EBI-739648From a different organism.
KRTAP26-1Q6PEX34EBI-3957603,EBI-3957672From a different organism.
KRTAP3-2Q9BYR74EBI-3957603,EBI-751260From a different organism.
KRTAP4-12Q9BQ664EBI-3957603,EBI-739863From a different organism.
KRTAP5-9P263713EBI-3957603,EBI-3958099From a different organism.
LGALS13Q9UHV83EBI-3957603,EBI-3957707From a different organism.
LIMS1P480593EBI-3957603,EBI-306928From a different organism.
LNX2Q8N4483EBI-3957603,EBI-2340947From a different organism.
LPXNO607113EBI-3957603,EBI-744222From a different organism.
MDFIQ997503EBI-3957603,EBI-724076From a different organism.
MGAT5BQ3V5L53EBI-3957603,EBI-3957727From a different organism.
N4BP2L2Q928023EBI-3957603,EBI-2514973From a different organism.
OGTO152943EBI-3957603,EBI-539828From a different organism.
PDCD6IPQ8WUM43EBI-3957603,EBI-310624From a different organism.
PDLIM7Q9NR123EBI-3957603,EBI-350517From a different organism.
PITX2Q996973EBI-3957603,EBI-1175211From a different organism.
PLSCR1O151623EBI-3957603,EBI-740019From a different organism.
PLSCR4Q9NRQ24EBI-3957603,EBI-769257From a different organism.
PRDM14Q9GZV83EBI-3957603,EBI-3957793From a different organism.
PRRC2BQ5JSZ53EBI-3957603,EBI-744891From a different organism.
RAB33AQ140883EBI-3957603,EBI-744685From a different organism.
RBCK1Q9BYM83EBI-3957603,EBI-2340624From a different organism.
RBPMSQ930623EBI-3957603,EBI-740322From a different organism.
RGS20O760813EBI-3957603,EBI-1052678From a different organism.
SMOC1Q9H4F82EBI-3957603,EBI-2801103From a different organism.
SPRY1O436093EBI-3957603,EBI-3866665From a different organism.
TRAF1Q130774EBI-3957603,EBI-359224From a different organism.
TRAF2Q129333EBI-3957603,EBI-355744From a different organism.
TRAPPC6AO758653EBI-3957603,EBI-743573From a different organism.
TRIM23P364062EBI-3957603,EBI-740098From a different organism.
TRIP6Q156543EBI-3957603,EBI-742327From a different organism.
ZBTB16Q055163EBI-3957603,EBI-711925From a different organism.
ZBTB32Q9Y2Y43EBI-3957603,EBI-954098From a different organism.
ZZZ3Q8IYH53EBI-3957603,EBI-2795524From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform ERA-1-993 (identifier: P09022-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform ERA-1-399 (identifier: P09022-2)

The sequence of this isoform differs from the canonical sequence as follows:
     115-133: VSGGYPPCAPAVYSGNLST → PPRSLSFPCFRDVFSSADL
     134-331: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331Homeobox protein Hox-A1
PRO_0000200031

Regions

DNA binding225 – 28460Homeobox
Motif200 – 2056Antp-type hexapeptide
Compositional bias60 – 7011Poly-His
Compositional bias138 – 1425Poly-His

Natural variations

Alternative sequence115 – 13319VSGGY…GNLST → PPRSLSFPCFRDVFSSADL in isoform ERA-1-399.
VSP_002380
Alternative sequence134 – 331198Missing in isoform ERA-1-399.
VSP_002381

Sequences

Sequence LengthMass (Da)Tools
Isoform ERA-1-993 [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: F73E4FFB1C6F15B2

FASTA33136,037
        10         20         30         40         50         60 
MNSFLEYPIL GSGDSGTCSA RAYPSDHGIT TFQSCAVSAN SCGGDDRFLV GRGVQISSPH 

        70         80         90        100        110        120 
HHHHHHHHHH PQTATYQTSG NLGISYSHSS CGPSYGAQNF SAPYGPYGLN QEADVSGGYP 

       130        140        150        160        170        180 
PCAPAVYSGN LSTPMVQHHH HHQGYAGGTV GSPQYIHHSY GQEQQTLALA TYNNSLSPLH 

       190        200        210        220        230        240 
ASHQEACRSP ASETSSPAQT FDWMKVKRNP PKTGKVGEYG YVGQPNAVRT NFTTKQLTEL 

       250        260        270        280        290        300 
EKEFHFNKYL TRARRVEIAA SLQLNETQVK IWFQNRRMKQ KKREKEGLLP ISPATPPGSD 

       310        320        330 
EKTEESSEKS SPSPSAPSPA SSTSDTLTTS H 

« Hide

Isoform ERA-1-399 [UniParc].

Checksum: F3A12654DA61A299
Show »

FASTA13314,290

References

[1]"Early retinoic acid-induced F9 teratocarcinoma stem cell gene ERA-1: alternate splicing creates transcripts for a homeobox-containing protein and one lacking the homeobox."
Larosa G.J., Gudas L.J.
Mol. Cell. Biol. 8:3906-3917(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Hox-1.6: a mouse homeo-box-containing gene member of the Hox-1 complex."
Baron A., Featherstone M.S., Hill R.E., Hall A., Galliott B., Duboule D.
EMBO J. 6:2977-2986(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 204-331.
[3]"The structural and functional organization of the murine HOX gene family resembles that of Drosophila homeotic genes."
Duboule D., Dolle P.
EMBO J. 8:1497-1505(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22115 mRNA. Translation: AAA37559.1.
M22115 mRNA. Translation: AAA37558.1.
X06023 Genomic DNA. Translation: CAA29426.1.
X06024 Genomic DNA. Translation: CAA29427.1.
M20214 Genomic DNA. No translation available.
M20216, M20215 Genomic DNA. Translation: AAA37839.1.
PIRA27155.
A30242.
B30242.
UniGeneMm.197.

3D structure databases

ProteinModelPortalP09022.
SMRP09022. Positions 198-286.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59869N.
IntActP09022. 61 interactions.

PTM databases

PhosphoSiteP09022.

Proteomic databases

PRIDEP09022.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:96170. Hoxa1.

Phylogenomic databases

eggNOGNOG236971.
HOGENOMHOG000171545.
HOVERGENHBG006089.
InParanoidP09022.

Gene expression databases

ArrayExpressP09022.
BgeeP09022.
CleanExMM_HOXA1.
GenevestigatorP09022.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP09022.
SOURCESearch...

Entry information

Entry nameHXA1_MOUSE
AccessionPrimary (citable) accession number: P09022
Secondary accession number(s): P15463
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Vertebrate homeotic Hox proteins

Nomenclature of vertebrate homeotic Hox proteins and list of entries