ID   HME2A_DANRE             Reviewed;         265 AA.
AC   P09015;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 2.
DT   27-MAR-2024, entry version 173.
DE   RecName: Full=Homeobox protein engrailed-2a;
DE            Short=Homeobox protein en-2a;
DE   AltName: Full=Zf-En-2;
GN   Name=eng2a; Synonyms=eng-2, eng2, zf-en-2;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1362650; DOI=10.1016/0925-4773(92)90025-f;
RA   Fjose A., Njolstad P.R., Nornes S., Molven A., Krauss S.;
RT   "Structure and early embryonic expression of the zebrafish engrailed-2
RT   gene.";
RL   Mech. Dev. 39:51-62(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1363539; DOI=10.1242/dev.116.4.1001;
RA   Ekker M., Wegner J., Akimenko M.-A., Westerfield M.;
RT   "Coordinate embryonic expression of three zebrafish engrailed genes.";
RL   Development 116:1001-1010(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 162-265.
RX   PubMed=2896133; DOI=10.1016/0014-5793(88)80849-4;
RA   Fjose A., Eiken H.G., Njolstad P.R., Molven A., Hordvik I.;
RT   "A zebrafish engrailed-like homeobox sequence expressed during
RT   embryogenesis.";
RL   FEBS Lett. 231:355-360(1988).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 182-244.
RX   PubMed=1980115; DOI=10.1016/0014-5793(90)80858-g;
RA   Holland P.W.H., Williams N.A.;
RT   "Conservation of engrailed-like homeobox sequences during vertebrate
RT   evolution.";
RL   FEBS Lett. 277:250-252(1990).
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the engrailed homeobox family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA30471.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X68151; CAA48254.1; -; Genomic_DNA.
DR   EMBL; X68446; CAA48491.1; -; mRNA.
DR   EMBL; X07622; CAA30471.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X59126; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A56557; A56557.
DR   PIR; S00562; S00562.
DR   RefSeq; NP_571119.2; NM_131044.2.
DR   AlphaFoldDB; P09015; -.
DR   BMRB; P09015; -.
DR   SMR; P09015; -.
DR   STRING; 7955.ENSDARP00000028905; -.
DR   PaxDb; 7955-ENSDARP00000028905; -.
DR   GeneID; 30243; -.
DR   KEGG; dre:30243; -.
DR   AGR; ZFIN:ZDB-GENE-980526-167; -.
DR   CTD; 30243; -.
DR   ZFIN; ZDB-GENE-980526-167; en2a.
DR   eggNOG; KOG0493; Eukaryota.
DR   HOGENOM; CLU_051739_0_0_1; -.
DR   InParanoid; P09015; -.
DR   OMA; GESGDQC; -.
DR   OrthoDB; 2897502at2759; -.
DR   PhylomeDB; P09015; -.
DR   TreeFam; TF106461; -.
DR   PRO; PR:P09015; -.
DR   Proteomes; UP000000437; Alternate scaffold 7.
DR   Proteomes; UP000000437; Chromosome 7.
DR   Bgee; ENSDARG00000026599; Expressed in brain and 6 other cell types or tissues.
DR   ExpressionAtlas; P09015; baseline.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IGI:ZFIN.
DR   GO; GO:0060561; P:apoptotic process involved in morphogenesis; IGI:ZFIN.
DR   GO; GO:0060385; P:axonogenesis involved in innervation; IGI:ZFIN.
DR   GO; GO:0001708; P:cell fate specification; IGI:ZFIN.
DR   GO; GO:0030901; P:midbrain development; IGI:ZFIN.
DR   GO; GO:0030917; P:midbrain-hindbrain boundary development; IGI:ZFIN.
DR   GO; GO:0021555; P:midbrain-hindbrain boundary morphogenesis; IGI:ZFIN.
DR   GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR019549; Homeobox-engrailed_C-terminal.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR000747; Homeobox_engrailed.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR019737; Homoebox-engrailed_CS.
DR   InterPro; IPR000047; HTH_motif.
DR   PANTHER; PTHR24341; HOMEOBOX PROTEIN ENGRAILED; 1.
DR   PANTHER; PTHR24341:SF5; HOMEOBOX PROTEIN ENGRAILED-2; 1.
DR   Pfam; PF10525; Engrail_1_C_sig; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00026; ENGRAILED.
DR   PRINTS; PR00024; HOMEOBOX.
DR   PRINTS; PR00031; HTHREPRESSR.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00033; ENGRAILED; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..265
FT                   /note="Homeobox protein engrailed-2a"
FT                   /id="PRO_0000196073"
FT   DNA_BIND        176..235
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          65..142
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          155..180
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        73..102
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        103..117
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        123..142
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        206
FT                   /note="R -> A (in Ref. 3; CAA30471)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        265
FT                   /note="D -> H (in Ref. 2; CAA48491)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   265 AA;  29540 MW;  AB4B08EF062D5AE0 CRC64;
     MDENEQSARD VEQRGASDES NSAIRPLLQA PGNLQLPHRI TNFFIDNILR PDFGRKKEAN
     ITRYEDNHGA RENHNPTGPS TGQVGSTVPA EEASTTHTSS GGKEAEIESE EPLKPRGENV
     DQCLGSESDS SQSNSNGQTG QGMLWPAWVY CTRYSDRPSS GPRSRKPKKK AASKEDKRPR
     TAFTAEQLQR LKAEFQTNRY LTEQRRQSLA QELGLNESQI KIWFQNKRAK IKKASGVKNG
     LAIHLMAQGL YNHSTTSKED KSDSD
//