P09007 (SRD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-rRNA-processing protein SRD1

Alternative name(s):
Suppressor of rRNA-processing defect protein 1

Gene names

Name:	SRD1
Ordered Locus Names:	YCR018C
ORF Names:	YCR18C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a direct or indirect role in pre-rRNA processing. Ref.5
Subcellular location	Cytoplasm. Nucleus Ref.7.
Miscellaneous	Present with 2100 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 GATA-type zinc finger.
Sequence caution	The sequence CAA29638.1 differs from that shown. Reason: Frameshift at position 218.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA processing Inferred from genetic interaction Ref.5. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.7. Source: SGD nucleus Inferred from direct assay Ref.7. Source: SGD
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 19111667PubMed 19158363. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Pre-rRNA-processing protein SRD1		PRO_0000083482
Regions
	Zinc finger	168 – 193	26	GATA-type
Experimental info
	Sequence conflict	106	1	T → I in CAA29638. Ref.1
	Sequence conflict	192	1	P → R in CAA30298. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P09007 [UniParc]. Last modified March 15, 2004. Version 2. Checksum: D9519E6564546687	FASTA	221	25,514
	10 20 30 40 50 60 MRYNNYDNSG SSFLTRVVKK SDMEKTLLLN REIDDWKSND KKKAYKERGR VYASCSFIEV 70 80 90 100 110 120 SFSQIRAVDV EKKIENAEQL RDLTRNIVKN KTSSLNEITP SKNRVTSACN SERRTTSQEA 130 140 150 160 170 180 NNLEGYHSCA QGTSRSASIT KKYSKKTTSR PKREKRQTIL PNGEIKECSK CKDTWTIQWR 190 200 210 220 SGPDQNRELC SPCGLAYGKR LKKENEKKRQ AADKRIDRNN P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Polymorphisms on the right arm of yeast chromosome III associated with Ty transposition and recombination events." Warmington J.R., Green R.P., Newlon C.S., Oliver S.G. Nucleic Acids Res. 15:8963-8982(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CN31C and S288c / GRF88.
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Valles G., Volckaerts G. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 106 AND C-TERMINUS.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"SRD1, a Saccharomyces cerevisiae suppressor of the temperature-sensitive pre-rRNA processing defect of rrp1-1." Fabian G.R., Hess S.M., Hopper A.K. Genetics 124:497-504(1990) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"SRD1, a S. cerevisiae gene affecting pre-rRNA processing contains a C2/C2 zinc finger motif." Hess S.M., Stanford D.R., Hopper A.K. Nucleic Acids Res. 22:1265-1271(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06322 Genomic DNA. Translation: CAA29638.1. Frameshift. X07388 Genomic DNA. Translation: CAA30298.1. Sequence problems. X59720 Genomic DNA. Translation: CAA42309.2. BK006937 Genomic DNA. Translation: DAA07495.1.
PIR	S07396. S19428.
RefSeq	NP_009944.2. NM_001178731.1.
3D structure databases
ProteinModelPortal	P09007.
SMR	P09007. Positions 164-201.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4478353.
Proteomic databases
PaxDb	P09007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR018C; YCR018C; YCR018C.
GeneID	850377.
KEGG	sce:YCR018C.
Organism-specific databases
CYGD	YCR018c.
SGD	S000000611. SRD1.
Phylogenomic databases
eggNOG	NOG311416.
GeneTree	ENSGT00530000068273.
KO	K14858.
OMA	CNSERRT.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29333-MONOMER.
Gene expression databases
Genevestigator	P09007.
GermOnline	YCR018C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.50.10. 1 hit.
InterPro	IPR000679. Znf_GATA. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF00320. GATA. 1 hit. [Graphical view]
SMART	SM00401. ZnF_GATA. 1 hit. [Graphical view]
PROSITE	PS00344. GATA_ZN_FINGER_1. False negative. PS50114. GATA_ZN_FINGER_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	965878.

Entry information

Entry name

SRD1_YEAST

Accession

Primary (citable) accession number: P09007
Secondary accession number(s): D6VR26, P09008, Q06989

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	March 15, 2004
Last modified:	May 29, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program