ID MASY_ECOLI Reviewed; 533 AA. AC P08997; Q2M6U0; DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1988, sequence version 1. DT 24-JAN-2024, entry version 172. DE RecName: Full=Malate synthase A; DE Short=MSA; DE EC=2.3.3.9; GN Name=aceB; Synonyms=mas; OrderedLocusNames=b4014, JW3974; OS Escherichia coli (strain K12). OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=K12; RX PubMed=3050899; DOI=10.1093/nar/16.19.9342; RA Byrne C.R., Stokes H.W., Ward K.A.; RT "Nucleotide sequence of the aceB gene encoding malate synthase A in RT Escherichia coli."; RL Nucleic Acids Res. 16:9342-9342(1988). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=3060852; DOI=10.1093/nar/16.22.10924; RA Byrne C.R., Stokes H.W., Ward K.A.; RT "Nucleotide sequence of the aceB gene encoding malate synthase A in RT Escherichia coli."; RL Nucleic Acids Res. 16:10924-10924(1988). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX PubMed=8265357; DOI=10.1093/nar/21.23.5408; RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.; RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the region RT from 89.2 to 92.8 minutes."; RL Nucleic Acids Res. 21:5408-5417(1993). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., RA Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1462(1997). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 RT and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+); CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57288; EC=2.3.3.9; CC -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from CC isocitrate: step 2/2. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X12431; CAA30973.1; -; Genomic_DNA. DR EMBL; U00006; AAC43108.1; -; Genomic_DNA. DR EMBL; U00096; AAC76984.1; -; Genomic_DNA. DR EMBL; AP009048; BAE78016.1; -; Genomic_DNA. DR PIR; A32649; SYECMA. DR RefSeq; NP_418438.1; NC_000913.3. DR RefSeq; WP_000138905.1; NZ_SSZK01000049.1. DR PDB; 3CUZ; X-ray; 1.04 A; A=2-533. DR PDB; 3CV1; X-ray; 1.68 A; A=2-533. DR PDB; 3CV2; X-ray; 1.40 A; A/B=2-533. DR PDBsum; 3CUZ; -. DR PDBsum; 3CV1; -. DR PDBsum; 3CV2; -. DR AlphaFoldDB; P08997; -. DR SMR; P08997; -. DR BioGRID; 4259288; 6. DR IntAct; P08997; 6. DR STRING; 511145.b4014; -. DR jPOST; P08997; -. DR PaxDb; 511145-b4014; -. DR EnsemblBacteria; AAC76984; AAC76984; b4014. DR GeneID; 948512; -. DR KEGG; ecj:JW3974; -. DR KEGG; eco:b4014; -. DR PATRIC; fig|1411691.4.peg.2699; -. DR EchoBASE; EB0022; -. DR eggNOG; COG2225; Bacteria. DR HOGENOM; CLU_018928_3_0_6; -. DR InParanoid; P08997; -. DR OMA; CHKRGAP; -. DR OrthoDB; 9768429at2; -. DR PhylomeDB; P08997; -. DR BioCyc; EcoCyc:MALATE-SYNTHASE; -. DR BioCyc; MetaCyc:MALATE-SYNTHASE; -. DR UniPathway; UPA00703; UER00720. DR EvolutionaryTrace; P08997; -. DR PRO; PR:P08997; -. DR Proteomes; UP000000318; Chromosome. DR Proteomes; UP000000625; Chromosome. DR GO; GO:0005737; C:cytoplasm; IDA:EcoliWiki. DR GO; GO:0004474; F:malate synthase activity; IDA:EcoCyc. DR GO; GO:0006097; P:glyoxylate cycle; IBA:GO_Central. DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. DR CDD; cd00727; malate_synt_A; 1. DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1. DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1. DR InterPro; IPR044856; Malate_synth_C_sf. DR InterPro; IPR011076; Malate_synth_sf. DR InterPro; IPR006252; Malate_synthA. DR InterPro; IPR019830; Malate_synthase_CS. DR InterPro; IPR001465; Malate_synthase_TIM. DR InterPro; IPR048355; MS_C. DR InterPro; IPR048356; MS_N. DR InterPro; IPR046363; MS_N_TIM-barrel_dom. DR NCBIfam; TIGR01344; malate_syn_A; 1. DR PANTHER; PTHR42902; MALATE SYNTHASE; 1. DR PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1. DR Pfam; PF20659; MS_C; 1. DR Pfam; PF20656; MS_N; 1. DR Pfam; PF01274; MS_TIM-barrel; 1. DR PIRSF; PIRSF001363; Malate_synth; 1. DR SUPFAM; SSF51645; Malate synthase G; 1. DR PROSITE; PS00510; MALATE_SYNTHASE; 1. PE 1: Evidence at protein level; KW 3D-structure; Cytoplasm; Glyoxylate bypass; Reference proteome; KW Transferase; Tricarboxylic acid cycle. FT CHAIN 1..533 FT /note="Malate synthase A" FT /id="PRO_0000166875" FT ACT_SITE 166 FT /note="Proton acceptor" FT /evidence="ECO:0000250" FT ACT_SITE 447 FT /note="Proton donor" FT /evidence="ECO:0000250" FT STRAND 12..15 FT /evidence="ECO:0007829|PDB:3CV2" FT HELIX 19..24 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 27..59 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 71..74 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 85..87 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 91..97 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 100..107 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 109..119 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 126..140 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 145..147 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 153..155 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 162..166 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 173..179 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 182..185 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 186..204 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 210..213 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 219..235 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 243..248 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 252..255 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 258..264 FT /evidence="ECO:0007829|PDB:3CUZ" FT TURN 265..268 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 269..273 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 277..286 FT /evidence="ECO:0007829|PDB:3CUZ" FT TURN 287..289 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 291..293 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 298..300 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 306..321 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 325..331 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 339..341 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 343..359 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 362..368 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 369..371 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 372..383 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 402..406 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 415..433 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 438..441 FT /evidence="ECO:0007829|PDB:3CUZ" FT STRAND 444..446 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 448..463 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 476..494 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 496..501 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 504..516 FT /evidence="ECO:0007829|PDB:3CUZ" FT HELIX 525..529 FT /evidence="ECO:0007829|PDB:3CUZ" SQ SEQUENCE 533 AA; 60274 MW; EB88388D7E4C5098 CRC64; MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA RIQQQQDIDN GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER KMVINALNAN VKVFMADFED SLAPDWNKVI DGQINLRDAV NGTISYTNEA GKIYQLKPNP AVLICRVRGL HLPEKHVTWR GEAIPGSLFD FALYFFHNYQ ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR GTIKATLLIE TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK ADKSLEANNG HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI TADQLLAPCD GERTEEGMRA NIRVAVQYIE AWISGNGCVP IYGLMEDAAT AEISRTSIWQ WIHHQKTLSN GKPVTKALFR QMLGEEMKVI ASELGEERFS QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA //