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P08997

- MASY_ECOLI

UniProt

P08997 - MASY_ECOLI

Protein

Malate synthase A

Gene

aceB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei166 – 1661Proton acceptorBy similarity
    Active sitei447 – 4471Proton donorBy similarity

    GO - Molecular functioni

    1. malate synthase activity Source: EcoliWiki

    GO - Biological processi

    1. glyoxylate cycle Source: EcoliWiki
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    BioCyciEcoCyc:MALATE-SYNTHASE.
    ECOL316407:JW3974-MONOMER.
    MetaCyc:MALATE-SYNTHASE.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase A (EC:2.3.3.9)
    Short name:
    MSA
    Gene namesi
    Name:aceB
    Synonyms:mas
    Ordered Locus Names:b4014, JW3974
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10023. aceB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: EcoliWiki

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 533533Malate synthase APRO_0000166875Add
    BLAST

    Proteomic databases

    PaxDbiP08997.
    PRIDEiP08997.

    Expressioni

    Gene expression databases

    GenevestigatoriP08997.

    Interactioni

    Protein-protein interaction databases

    IntActiP08997. 6 interactions.
    STRINGi511145.b4014.

    Structurei

    Secondary structure

    1
    533
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi12 – 154
    Helixi19 – 246
    Helixi27 – 5933
    Helixi71 – 744
    Helixi85 – 873
    Beta strandi91 – 977
    Helixi100 – 1078
    Beta strandi109 – 11911
    Helixi126 – 14015
    Beta strandi145 – 1473
    Beta strandi153 – 1553
    Beta strandi162 – 1665
    Beta strandi173 – 1797
    Beta strandi182 – 1854
    Helixi186 – 20419
    Beta strandi210 – 2134
    Helixi219 – 23517
    Beta strandi243 – 2486
    Helixi252 – 2554
    Helixi258 – 2647
    Turni265 – 2684
    Beta strandi269 – 2735
    Helixi277 – 28610
    Turni287 – 2893
    Helixi291 – 2933
    Helixi298 – 3003
    Helixi306 – 32116
    Beta strandi325 – 3317
    Helixi339 – 3413
    Helixi343 – 35917
    Beta strandi362 – 3687
    Helixi369 – 3713
    Helixi372 – 38312
    Helixi402 – 4065
    Helixi415 – 43319
    Beta strandi438 – 4414
    Beta strandi444 – 4463
    Helixi448 – 46316
    Helixi476 – 49419
    Helixi496 – 5016
    Helixi504 – 51613
    Helixi525 – 5295

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3CUZX-ray1.04A2-533[»]
    3CV1X-ray1.68A2-533[»]
    3CV2X-ray1.40A/B2-533[»]
    ProteinModelPortaliP08997.
    SMRiP08997. Positions 5-533.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08997.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the malate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000238464.
    KOiK01638.
    OMAiSIWQWIR.
    OrthoDBiEOG69WFFX.
    PhylomeDBiP08997.

    Family and domain databases

    InterProiIPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001363. Malate_synth. 1 hit.
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
    PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08997-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA    50
    RIQQQQDIDN GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER 100
    KMVINALNAN VKVFMADFED SLAPDWNKVI DGQINLRDAV NGTISYTNEA 150
    GKIYQLKPNP AVLICRVRGL HLPEKHVTWR GEAIPGSLFD FALYFFHNYQ 200
    ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR GTIKATLLIE 250
    TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA 300
    VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK 350
    ADKSLEANNG HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI 400
    TADQLLAPCD GERTEEGMRA NIRVAVQYIE AWISGNGCVP IYGLMEDAAT 450
    AEISRTSIWQ WIHHQKTLSN GKPVTKALFR QMLGEEMKVI ASELGEERFS 500
    QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA 533
    Length:533
    Mass (Da):60,274
    Last modified:November 1, 1988 - v1
    Checksum:iEB88388D7E4C5098
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12431 Genomic DNA. Translation: CAA30973.1.
    U00006 Genomic DNA. Translation: AAC43108.1.
    U00096 Genomic DNA. Translation: AAC76984.1.
    AP009048 Genomic DNA. Translation: BAE78016.1.
    PIRiA32649. SYECMA.
    RefSeqiNP_418438.1. NC_000913.3.
    YP_492157.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76984; AAC76984; b4014.
    BAE78016; BAE78016; BAE78016.
    GeneIDi12930294.
    948512.
    KEGGiecj:Y75_p3901.
    eco:b4014.
    PATRICi32123555. VBIEscCol129921_4126.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12431 Genomic DNA. Translation: CAA30973.1 .
    U00006 Genomic DNA. Translation: AAC43108.1 .
    U00096 Genomic DNA. Translation: AAC76984.1 .
    AP009048 Genomic DNA. Translation: BAE78016.1 .
    PIRi A32649. SYECMA.
    RefSeqi NP_418438.1. NC_000913.3.
    YP_492157.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3CUZ X-ray 1.04 A 2-533 [» ]
    3CV1 X-ray 1.68 A 2-533 [» ]
    3CV2 X-ray 1.40 A/B 2-533 [» ]
    ProteinModelPortali P08997.
    SMRi P08997. Positions 5-533.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08997. 6 interactions.
    STRINGi 511145.b4014.

    Proteomic databases

    PaxDbi P08997.
    PRIDEi P08997.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76984 ; AAC76984 ; b4014 .
    BAE78016 ; BAE78016 ; BAE78016 .
    GeneIDi 12930294.
    948512.
    KEGGi ecj:Y75_p3901.
    eco:b4014.
    PATRICi 32123555. VBIEscCol129921_4126.

    Organism-specific databases

    EchoBASEi EB0022.
    EcoGenei EG10023. aceB.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000238464.
    KOi K01638.
    OMAi SIWQWIR.
    OrthoDBi EOG69WFFX.
    PhylomeDBi P08997.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci EcoCyc:MALATE-SYNTHASE.
    ECOL316407:JW3974-MONOMER.
    MetaCyc:MALATE-SYNTHASE.

    Miscellaneous databases

    EvolutionaryTracei P08997.
    PROi P08997.

    Gene expression databases

    Genevestigatori P08997.

    Family and domain databases

    InterProi IPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001363. Malate_synth. 1 hit.
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
    PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli."
      Byrne C.R., Stokes H.W., Ward K.A.
      Nucleic Acids Res. 16:9342-9342(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli."
      Byrne C.R., Stokes H.W., Ward K.A.
      Nucleic Acids Res. 16:10924-10924(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
      Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
      Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiMASY_ECOLI
    AccessioniPrimary (citable) accession number: P08997
    Secondary accession number(s): Q2M6U0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3