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P08997

- MASY_ECOLI

UniProt

P08997 - MASY_ECOLI

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Protein

Malate synthase A

Gene

aceB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661Proton acceptorBy similarity
Active sitei447 – 4471Proton donorBy similarity

GO - Molecular functioni

  1. malate synthase activity Source: EcoliWiki

GO - Biological processi

  1. glyoxylate cycle Source: EcoliWiki
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciEcoCyc:MALATE-SYNTHASE.
ECOL316407:JW3974-MONOMER.
MetaCyc:MALATE-SYNTHASE.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase A (EC:2.3.3.9)
Short name:
MSA
Gene namesi
Name:aceB
Synonyms:mas
Ordered Locus Names:b4014, JW3974
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10023. aceB.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Malate synthase APRO_0000166875Add
BLAST

Proteomic databases

PaxDbiP08997.
PRIDEiP08997.

Expressioni

Gene expression databases

GenevestigatoriP08997.

Interactioni

Protein-protein interaction databases

IntActiP08997. 6 interactions.
STRINGi511145.b4014.

Structurei

Secondary structure

1
533
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 154Combined sources
Helixi19 – 246Combined sources
Helixi27 – 5933Combined sources
Helixi71 – 744Combined sources
Helixi85 – 873Combined sources
Beta strandi91 – 977Combined sources
Helixi100 – 1078Combined sources
Beta strandi109 – 11911Combined sources
Helixi126 – 14015Combined sources
Beta strandi145 – 1473Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi162 – 1665Combined sources
Beta strandi173 – 1797Combined sources
Beta strandi182 – 1854Combined sources
Helixi186 – 20419Combined sources
Beta strandi210 – 2134Combined sources
Helixi219 – 23517Combined sources
Beta strandi243 – 2486Combined sources
Helixi252 – 2554Combined sources
Helixi258 – 2647Combined sources
Turni265 – 2684Combined sources
Beta strandi269 – 2735Combined sources
Helixi277 – 28610Combined sources
Turni287 – 2893Combined sources
Helixi291 – 2933Combined sources
Helixi298 – 3003Combined sources
Helixi306 – 32116Combined sources
Beta strandi325 – 3317Combined sources
Helixi339 – 3413Combined sources
Helixi343 – 35917Combined sources
Beta strandi362 – 3687Combined sources
Helixi369 – 3713Combined sources
Helixi372 – 38312Combined sources
Helixi402 – 4065Combined sources
Helixi415 – 43319Combined sources
Beta strandi438 – 4414Combined sources
Beta strandi444 – 4463Combined sources
Helixi448 – 46316Combined sources
Helixi476 – 49419Combined sources
Helixi496 – 5016Combined sources
Helixi504 – 51613Combined sources
Helixi525 – 5295Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUZX-ray1.04A2-533[»]
3CV1X-ray1.68A2-533[»]
3CV2X-ray1.40A/B2-533[»]
ProteinModelPortaliP08997.
SMRiP08997. Positions 5-533.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08997.

Family & Domainsi

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000238464.
InParanoidiP08997.
KOiK01638.
OMAiSIWQWIR.
OrthoDBiEOG69WFFX.
PhylomeDBiP08997.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08997-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA
60 70 80 90 100
RIQQQQDIDN GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER
110 120 130 140 150
KMVINALNAN VKVFMADFED SLAPDWNKVI DGQINLRDAV NGTISYTNEA
160 170 180 190 200
GKIYQLKPNP AVLICRVRGL HLPEKHVTWR GEAIPGSLFD FALYFFHNYQ
210 220 230 240 250
ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR GTIKATLLIE
260 270 280 290 300
TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA
310 320 330 340 350
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK
360 370 380 390 400
ADKSLEANNG HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI
410 420 430 440 450
TADQLLAPCD GERTEEGMRA NIRVAVQYIE AWISGNGCVP IYGLMEDAAT
460 470 480 490 500
AEISRTSIWQ WIHHQKTLSN GKPVTKALFR QMLGEEMKVI ASELGEERFS
510 520 530
QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA
Length:533
Mass (Da):60,274
Last modified:November 1, 1988 - v1
Checksum:iEB88388D7E4C5098
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12431 Genomic DNA. Translation: CAA30973.1.
U00006 Genomic DNA. Translation: AAC43108.1.
U00096 Genomic DNA. Translation: AAC76984.1.
AP009048 Genomic DNA. Translation: BAE78016.1.
PIRiA32649. SYECMA.
RefSeqiNP_418438.1. NC_000913.3.
YP_492157.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76984; AAC76984; b4014.
BAE78016; BAE78016; BAE78016.
GeneIDi12930294.
948512.
KEGGiecj:Y75_p3901.
eco:b4014.
PATRICi32123555. VBIEscCol129921_4126.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12431 Genomic DNA. Translation: CAA30973.1 .
U00006 Genomic DNA. Translation: AAC43108.1 .
U00096 Genomic DNA. Translation: AAC76984.1 .
AP009048 Genomic DNA. Translation: BAE78016.1 .
PIRi A32649. SYECMA.
RefSeqi NP_418438.1. NC_000913.3.
YP_492157.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3CUZ X-ray 1.04 A 2-533 [» ]
3CV1 X-ray 1.68 A 2-533 [» ]
3CV2 X-ray 1.40 A/B 2-533 [» ]
ProteinModelPortali P08997.
SMRi P08997. Positions 5-533.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08997. 6 interactions.
STRINGi 511145.b4014.

Proteomic databases

PaxDbi P08997.
PRIDEi P08997.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76984 ; AAC76984 ; b4014 .
BAE78016 ; BAE78016 ; BAE78016 .
GeneIDi 12930294.
948512.
KEGGi ecj:Y75_p3901.
eco:b4014.
PATRICi 32123555. VBIEscCol129921_4126.

Organism-specific databases

EchoBASEi EB0022.
EcoGenei EG10023. aceB.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000238464.
InParanoidi P08997.
KOi K01638.
OMAi SIWQWIR.
OrthoDBi EOG69WFFX.
PhylomeDBi P08997.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci EcoCyc:MALATE-SYNTHASE.
ECOL316407:JW3974-MONOMER.
MetaCyc:MALATE-SYNTHASE.

Miscellaneous databases

EvolutionaryTracei P08997.
PROi P08997.

Gene expression databases

Genevestigatori P08997.

Family and domain databases

InterProi IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001363. Malate_synth. 1 hit.
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli."
    Byrne C.R., Stokes H.W., Ward K.A.
    Nucleic Acids Res. 16:9342-9342(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli."
    Byrne C.R., Stokes H.W., Ward K.A.
    Nucleic Acids Res. 16:10924-10924(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiMASY_ECOLI
AccessioniPrimary (citable) accession number: P08997
Secondary accession number(s): Q2M6U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 26, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3