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P08997

- MASY_ECOLI

UniProt

P08997 - MASY_ECOLI

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Protein

Malate synthase A

Gene
aceB, mas, b4014, JW3974
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661Proton acceptor By similarity
Active sitei447 – 4471Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: EcoliWiki

GO - Biological processi

  1. glyoxylate cycle Source: EcoliWiki
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciEcoCyc:MALATE-SYNTHASE.
ECOL316407:JW3974-MONOMER.
MetaCyc:MALATE-SYNTHASE.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase A (EC:2.3.3.9)
Short name:
MSA
Gene namesi
Name:aceB
Synonyms:mas
Ordered Locus Names:b4014, JW3974
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10023. aceB.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Malate synthase APRO_0000166875Add
BLAST

Proteomic databases

PaxDbiP08997.
PRIDEiP08997.

Expressioni

Gene expression databases

GenevestigatoriP08997.

Interactioni

Protein-protein interaction databases

IntActiP08997. 6 interactions.
STRINGi511145.b4014.

Structurei

Secondary structure

1
533
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 154
Helixi19 – 246
Helixi27 – 5933
Helixi71 – 744
Helixi85 – 873
Beta strandi91 – 977
Helixi100 – 1078
Beta strandi109 – 11911
Helixi126 – 14015
Beta strandi145 – 1473
Beta strandi153 – 1553
Beta strandi162 – 1665
Beta strandi173 – 1797
Beta strandi182 – 1854
Helixi186 – 20419
Beta strandi210 – 2134
Helixi219 – 23517
Beta strandi243 – 2486
Helixi252 – 2554
Helixi258 – 2647
Turni265 – 2684
Beta strandi269 – 2735
Helixi277 – 28610
Turni287 – 2893
Helixi291 – 2933
Helixi298 – 3003
Helixi306 – 32116
Beta strandi325 – 3317
Helixi339 – 3413
Helixi343 – 35917
Beta strandi362 – 3687
Helixi369 – 3713
Helixi372 – 38312
Helixi402 – 4065
Helixi415 – 43319
Beta strandi438 – 4414
Beta strandi444 – 4463
Helixi448 – 46316
Helixi476 – 49419
Helixi496 – 5016
Helixi504 – 51613
Helixi525 – 5295

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUZX-ray1.04A2-533[»]
3CV1X-ray1.68A2-533[»]
3CV2X-ray1.40A/B2-533[»]
ProteinModelPortaliP08997.
SMRiP08997. Positions 5-533.

Miscellaneous databases

EvolutionaryTraceiP08997.

Family & Domainsi

Sequence similaritiesi

Belongs to the malate synthase family.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000238464.
KOiK01638.
OMAiSIWQWIR.
OrthoDBiEOG69WFFX.
PhylomeDBiP08997.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08997-1 [UniParc]FASTAAdd to Basket

« Hide

MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA    50
RIQQQQDIDN GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER 100
KMVINALNAN VKVFMADFED SLAPDWNKVI DGQINLRDAV NGTISYTNEA 150
GKIYQLKPNP AVLICRVRGL HLPEKHVTWR GEAIPGSLFD FALYFFHNYQ 200
ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR GTIKATLLIE 250
TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA 300
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK 350
ADKSLEANNG HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI 400
TADQLLAPCD GERTEEGMRA NIRVAVQYIE AWISGNGCVP IYGLMEDAAT 450
AEISRTSIWQ WIHHQKTLSN GKPVTKALFR QMLGEEMKVI ASELGEERFS 500
QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA 533
Length:533
Mass (Da):60,274
Last modified:November 1, 1988 - v1
Checksum:iEB88388D7E4C5098
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12431 Genomic DNA. Translation: CAA30973.1.
U00006 Genomic DNA. Translation: AAC43108.1.
U00096 Genomic DNA. Translation: AAC76984.1.
AP009048 Genomic DNA. Translation: BAE78016.1.
PIRiA32649. SYECMA.
RefSeqiNP_418438.1. NC_000913.3.
YP_492157.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76984; AAC76984; b4014.
BAE78016; BAE78016; BAE78016.
GeneIDi12930294.
948512.
KEGGiecj:Y75_p3901.
eco:b4014.
PATRICi32123555. VBIEscCol129921_4126.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12431 Genomic DNA. Translation: CAA30973.1 .
U00006 Genomic DNA. Translation: AAC43108.1 .
U00096 Genomic DNA. Translation: AAC76984.1 .
AP009048 Genomic DNA. Translation: BAE78016.1 .
PIRi A32649. SYECMA.
RefSeqi NP_418438.1. NC_000913.3.
YP_492157.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3CUZ X-ray 1.04 A 2-533 [» ]
3CV1 X-ray 1.68 A 2-533 [» ]
3CV2 X-ray 1.40 A/B 2-533 [» ]
ProteinModelPortali P08997.
SMRi P08997. Positions 5-533.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08997. 6 interactions.
STRINGi 511145.b4014.

Proteomic databases

PaxDbi P08997.
PRIDEi P08997.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76984 ; AAC76984 ; b4014 .
BAE78016 ; BAE78016 ; BAE78016 .
GeneIDi 12930294.
948512.
KEGGi ecj:Y75_p3901.
eco:b4014.
PATRICi 32123555. VBIEscCol129921_4126.

Organism-specific databases

EchoBASEi EB0022.
EcoGenei EG10023. aceB.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000238464.
KOi K01638.
OMAi SIWQWIR.
OrthoDBi EOG69WFFX.
PhylomeDBi P08997.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci EcoCyc:MALATE-SYNTHASE.
ECOL316407:JW3974-MONOMER.
MetaCyc:MALATE-SYNTHASE.

Miscellaneous databases

EvolutionaryTracei P08997.
PROi P08997.

Gene expression databases

Genevestigatori P08997.

Family and domain databases

InterProi IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001363. Malate_synth. 1 hit.
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli."
    Byrne C.R., Stokes H.W., Ward K.A.
    Nucleic Acids Res. 16:9342-9342(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli."
    Byrne C.R., Stokes H.W., Ward K.A.
    Nucleic Acids Res. 16:10924-10924(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiMASY_ECOLI
AccessioniPrimary (citable) accession number: P08997
Secondary accession number(s): Q2M6U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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