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Protein

Malate synthase A

Gene

aceB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (aceA)
  2. Malate synthase G (glcB), Malate synthase A (aceB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166Proton acceptorBy similarity1
Active sitei447Proton donorBy similarity1

GO - Molecular functioni

  • malate synthase activity Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciEcoCyc:MALATE-SYNTHASE.
ECOL316407:JW3974-MONOMER.
MetaCyc:MALATE-SYNTHASE.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase A (EC:2.3.3.9)
Short name:
MSA
Gene namesi
Name:aceB
Synonyms:mas
Ordered Locus Names:b4014, JW3974
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10023. aceB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001668751 – 533Malate synthase AAdd BLAST533

Proteomic databases

PaxDbiP08997.
PRIDEiP08997.

Interactioni

Protein-protein interaction databases

BioGridi4259288. 3 interactors.
IntActiP08997. 6 interactors.
STRINGi511145.b4014.

Structurei

Secondary structure

1533
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 15Combined sources4
Helixi19 – 24Combined sources6
Helixi27 – 59Combined sources33
Helixi71 – 74Combined sources4
Helixi85 – 87Combined sources3
Beta strandi91 – 97Combined sources7
Helixi100 – 107Combined sources8
Beta strandi109 – 119Combined sources11
Helixi126 – 140Combined sources15
Beta strandi145 – 147Combined sources3
Beta strandi153 – 155Combined sources3
Beta strandi162 – 166Combined sources5
Beta strandi173 – 179Combined sources7
Beta strandi182 – 185Combined sources4
Helixi186 – 204Combined sources19
Beta strandi210 – 213Combined sources4
Helixi219 – 235Combined sources17
Beta strandi243 – 248Combined sources6
Helixi252 – 255Combined sources4
Helixi258 – 264Combined sources7
Turni265 – 268Combined sources4
Beta strandi269 – 273Combined sources5
Helixi277 – 286Combined sources10
Turni287 – 289Combined sources3
Helixi291 – 293Combined sources3
Helixi298 – 300Combined sources3
Helixi306 – 321Combined sources16
Beta strandi325 – 331Combined sources7
Helixi339 – 341Combined sources3
Helixi343 – 359Combined sources17
Beta strandi362 – 368Combined sources7
Helixi369 – 371Combined sources3
Helixi372 – 383Combined sources12
Helixi402 – 406Combined sources5
Helixi415 – 433Combined sources19
Beta strandi438 – 441Combined sources4
Beta strandi444 – 446Combined sources3
Helixi448 – 463Combined sources16
Helixi476 – 494Combined sources19
Helixi496 – 501Combined sources6
Helixi504 – 516Combined sources13
Helixi525 – 529Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CUZX-ray1.04A2-533[»]
3CV1X-ray1.68A2-533[»]
3CV2X-ray1.40A/B2-533[»]
ProteinModelPortaliP08997.
SMRiP08997.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08997.

Family & Domainsi

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000238464.
InParanoidiP08997.
KOiK01638.
OMAiCHKRGAP.
PhylomeDBiP08997.

Family and domain databases

CDDicd00727. malate_synt_A. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA
60 70 80 90 100
RIQQQQDIDN GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER
110 120 130 140 150
KMVINALNAN VKVFMADFED SLAPDWNKVI DGQINLRDAV NGTISYTNEA
160 170 180 190 200
GKIYQLKPNP AVLICRVRGL HLPEKHVTWR GEAIPGSLFD FALYFFHNYQ
210 220 230 240 250
ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR GTIKATLLIE
260 270 280 290 300
TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA
310 320 330 340 350
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK
360 370 380 390 400
ADKSLEANNG HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI
410 420 430 440 450
TADQLLAPCD GERTEEGMRA NIRVAVQYIE AWISGNGCVP IYGLMEDAAT
460 470 480 490 500
AEISRTSIWQ WIHHQKTLSN GKPVTKALFR QMLGEEMKVI ASELGEERFS
510 520 530
QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA
Length:533
Mass (Da):60,274
Last modified:November 1, 1988 - v1
Checksum:iEB88388D7E4C5098
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12431 Genomic DNA. Translation: CAA30973.1.
U00006 Genomic DNA. Translation: AAC43108.1.
U00096 Genomic DNA. Translation: AAC76984.1.
AP009048 Genomic DNA. Translation: BAE78016.1.
PIRiA32649. SYECMA.
RefSeqiNP_418438.1. NC_000913.3.
WP_000138905.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC76984; AAC76984; b4014.
BAE78016; BAE78016; BAE78016.
GeneIDi948512.
KEGGiecj:JW3974.
eco:b4014.
PATRICi32123555. VBIEscCol129921_4126.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12431 Genomic DNA. Translation: CAA30973.1.
U00006 Genomic DNA. Translation: AAC43108.1.
U00096 Genomic DNA. Translation: AAC76984.1.
AP009048 Genomic DNA. Translation: BAE78016.1.
PIRiA32649. SYECMA.
RefSeqiNP_418438.1. NC_000913.3.
WP_000138905.1. NZ_CP014272.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CUZX-ray1.04A2-533[»]
3CV1X-ray1.68A2-533[»]
3CV2X-ray1.40A/B2-533[»]
ProteinModelPortaliP08997.
SMRiP08997.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259288. 3 interactors.
IntActiP08997. 6 interactors.
STRINGi511145.b4014.

Proteomic databases

PaxDbiP08997.
PRIDEiP08997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76984; AAC76984; b4014.
BAE78016; BAE78016; BAE78016.
GeneIDi948512.
KEGGiecj:JW3974.
eco:b4014.
PATRICi32123555. VBIEscCol129921_4126.

Organism-specific databases

EchoBASEiEB0022.
EcoGeneiEG10023. aceB.

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000238464.
InParanoidiP08997.
KOiK01638.
OMAiCHKRGAP.
PhylomeDBiP08997.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciEcoCyc:MALATE-SYNTHASE.
ECOL316407:JW3974-MONOMER.
MetaCyc:MALATE-SYNTHASE.

Miscellaneous databases

EvolutionaryTraceiP08997.
PROiP08997.

Family and domain databases

CDDicd00727. malate_synt_A. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMASY_ECOLI
AccessioniPrimary (citable) accession number: P08997
Secondary accession number(s): Q2M6U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.