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P08997 (MASY_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase A

Short name=MSA
EC=2.3.3.9
Gene names
Name:aceB
Synonyms:mas
Ordered Locus Names:b4014, JW3974
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred by curator PubMed 4874804. Source: EcoliWiki

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 4874804. Source: EcoliWiki

   Molecular_functionmalate synthase activity

Inferred from direct assay PubMed 4874804. Source: EcoliWiki

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Malate synthase A
PRO_0000166875

Sites

Active site1661Proton acceptor By similarity
Active site4471Proton donor By similarity

Secondary structure

............................................................................... 533
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08997 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: EB88388D7E4C5098

FASTA53360,274
        10         20         30         40         50         60 
MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA RIQQQQDIDN 

        70         80         90        100        110        120 
GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER KMVINALNAN VKVFMADFED 

       130        140        150        160        170        180 
SLAPDWNKVI DGQINLRDAV NGTISYTNEA GKIYQLKPNP AVLICRVRGL HLPEKHVTWR 

       190        200        210        220        230        240 
GEAIPGSLFD FALYFFHNYQ ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR 

       250        260        270        280        290        300 
GTIKATLLIE TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA 

       310        320        330        340        350        360 
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK ADKSLEANNG 

       370        380        390        400        410        420 
HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI TADQLLAPCD GERTEEGMRA 

       430        440        450        460        470        480 
NIRVAVQYIE AWISGNGCVP IYGLMEDAAT AEISRTSIWQ WIHHQKTLSN GKPVTKALFR 

       490        500        510        520        530 
QMLGEEMKVI ASELGEERFS QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli."
Byrne C.R., Stokes H.W., Ward K.A.
Nucleic Acids Res. 16:9342-9342(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli."
Byrne C.R., Stokes H.W., Ward K.A.
Nucleic Acids Res. 16:10924-10924(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X12431 Genomic DNA. Translation: CAA30973.1.
U00006 Genomic DNA. Translation: AAC43108.1.
U00096 Genomic DNA. Translation: AAC76984.1.
AP009048 Genomic DNA. Translation: BAE78016.1.
PIRSYECMA. A32649.
RefSeqNP_418438.1. NC_000913.3.
YP_492157.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUZX-ray1.04A2-533[»]
3CV1X-ray1.68A2-533[»]
3CV2X-ray1.40A/B2-533[»]
ProteinModelPortalP08997.
SMRP08997. Positions 5-533.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP08997. 6 interactions.
STRING511145.b4014.

Proteomic databases

PaxDbP08997.
PRIDEP08997.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76984; AAC76984; b4014.
BAE78016; BAE78016; BAE78016.
GeneID12930294.
948512.
KEGGecj:Y75_p3901.
eco:b4014.
PATRIC32123555. VBIEscCol129921_4126.

Organism-specific databases

EchoBASEEB0022.
EcoGeneEG10023. aceB.

Phylogenomic databases

eggNOGCOG2225.
HOGENOMHOG000238464.
KOK01638.
OMASIWQWIR.
OrthoDBEOG69WFFX.
PhylomeDBP08997.

Enzyme and pathway databases

BioCycEcoCyc:MALATE-SYNTHASE.
ECOL316407:JW3974-MONOMER.
MetaCyc:MALATE-SYNTHASE.
UniPathwayUPA00703; UER00720.

Gene expression databases

GenevestigatorP08997.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP08997.
PROP08997.

Entry information

Entry nameMASY_ECOLI
AccessionPrimary (citable) accession number: P08997
Secondary accession number(s): Q2M6U0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene