Malate synthase A - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P08997 (MASY_ECOLI)

Last modified November 24, 2009. Version 87. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Malate synthase A
      Short name=MSA
    EC=2.3.3.9

Gene names

Name:	aceB
Synonyms:	mas
Ordered Locus Names:	b4014, JW3974

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	Acetyl-CoA + H₂O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the malate synthase family.

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Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
mukB	P22523	1	EBI-1113400,EBI-542943

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 533

533

Malate synthase A

PRO_0000166875

Sites

Active site

166

Proton acceptor By similarity

Active site

447

Proton donor By similarity

Secondary structure

533

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P08997-1 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: EB88388D7E4C5098
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FASTA

533

60,274

        10         20         30         40         50         60 
MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA RIQQQQDIDN 

        70         80         90        100        110        120 
GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER KMVINALNAN VKVFMADFED 

       130        140        150        160        170        180 
SLAPDWNKVI DGQINLRDAV NGTISYTNEA GKIYQLKPNP AVLICRVRGL HLPEKHVTWR 

       190        200        210        220        230        240 
GEAIPGSLFD FALYFFHNYQ ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR 

       250        260        270        280        290        300 
GTIKATLLIE TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA 

       310        320        330        340        350        360 
VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK ADKSLEANNG 

       370        380        390        400        410        420 
HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI TADQLLAPCD GERTEEGMRA 

       430        440        450        460        470        480 
NIRVAVQYIE AWISGNGCVP IYGLMEDAAT AEISRTSIWQ WIHHQKTLSN GKPVTKALFR 

       490        500        510        520        530 
QMLGEEMKVI ASELGEERFS QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli." Byrne C.R., Stokes H.W., Ward K.A. Nucleic Acids Res. 16:9342-9342(1988) [PubMed: 3050899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli." Byrne C.R., Stokes H.W., Ward K.A. Nucleic Acids Res. 16:10924-10924(1988) [PubMed: 3060852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X12431 Genomic DNA. Translation: CAA30973.1.
U00006 Genomic DNA. Translation: AAC43108.1.
U00096 Genomic DNA. Translation: AAC76984.1.
AP009048 Genomic DNA. Translation: BAE78016.1.

PIR

SYECMA. A32649.

RefSeq

AP_004515.1.
NP_418438.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CUZ	X-ray	1.04	A	2-533	[»]
3CV1	X-ray	1.68	A	2-533	[»]
3CV2	X-ray	1.40	A/B	2-533	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P08997. 6 interactions.

STRING

P08997.

Genome annotation databases

GeneID

948512.

GenomeReviews

Gene locus JW3974 in contig AP009048_GR.
Gene locus b4014 in contig U00096_GR.

KEGG

ecj:JW3974.
eco:b4014.

Organism-specific databases

EchoBASE

EB0022.

EcoGene

EG10023. aceB.

CMR

Search...

Phylogenomic databases

HOGENOM

P08997.

OMA

PVDRKMV

Enzyme and pathway databases

BioCyc

EcoCyc:MALATE-SYNTHASE.
ECOL168927:B4014-MON.
MetaCyc:MALATE-SYNTHASE.

Gene expression databases

Genevestigator

P08997.

Family and domain databases

InterPro

IPR011076. Malate_synth-like_core.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]

PANTHER

PTHR21631:SF1. Malate_synthase. 1 hit.

Pfam

PF01274. Malate_synthase. 1 hit.
[Graphical view]

PIRSF

PIRSF001363. Malate_synth. 1 hit.

TIGRFAMs

TIGR01344. malate_syn_A. 1 hit.

PROSITE

PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

MASY_ECOLI

Accession

Primary (citable) accession number: P08997
Secondary accession number(s): Q2M6U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	November 24, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents