Reviewed, UniProtKB/Swiss-Prot P08997 (MASY_ECOLI)

Names and origin

Protein names

Recommended name:
    Malate synthase A
      Short name=MSA
    EC=2.3.3.9

Gene names

Name:	aceB
Synonyms:	mas
Ordered Locus Names:	b4014, JW3974

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; L-malate from isocitrate: step 2/2.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mukB	P22523	1	EBI-1113400,EBI-542943

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	Malate synthase A		PRO_0000166875
Sites
	Active site	166	1	Proton acceptor By similarity
	Active site	447	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08997-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: EB88388D7E4C5098	FASTA	533	60,274
	10 20 30 40 50 60 MTEQATTTDE LAFTRPYGEQ EKQILTAEAV EFLTELVTHF TPQRNKLLAA RIQQQQDIDN 70 80 90 100 110 120 GTLPDFISET ASIRDADWKI RGIPADLEDR RVEITGPVER KMVINALNAN VKVFMADFED 130 140 150 160 170 180 SLAPDWNKVI DGQINLRDAV NGTISYTNEA GKIYQLKPNP AVLICRVRGL HLPEKHVTWR 190 200 210 220 230 240 GEAIPGSLFD FALYFFHNYQ ALLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YAEDRFNLPR 250 260 270 280 290 300 GTIKATLLIE TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQA 310 320 330 340 350 360 VTMDKPFLNA YSRLLIKTCH KRGAFAMGGM AAFIPSKDEE HNNQVLNKVK ADKSLEANNG 370 380 390 400 410 420 HDGTWIAHPG LADTAMAVFN DILGSRKNQL EVMREQDAPI TADQLLAPCD GERTEEGMRA 430 440 450 460 470 480 NIRVAVQYIE AWISGNGCVP IYGLMEDAAT AEISRTSIWQ WIHHQKTLSN GKPVTKALFR 490 500 510 520 530 QMLGEEMKVI ASELGEERFS QGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli." Byrne C.R., Stokes H.W., Ward K.A. Nucleic Acids Res. 16:9342-9342(1988) [PubMed: 3050899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli." Byrne C.R., Stokes H.W., Ward K.A. Nucleic Acids Res. 16:10924-10924(1988) [PubMed: 3060852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X12431 Genomic DNA. Translation: CAA30973.1.
U00006 Genomic DNA. Translation: AAC43108.1.
U00096 Genomic DNA. Translation: AAC76984.1.
AP009048 Genomic DNA. Translation: BAE78016.1.

PIR

SYECMA. A32649.

RefSeq

AP_004515.1.
NP_418438.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CUZ	X-ray	1.04	A	2-533	[»]
3CV1	X-ray	1.68	A	2-533	[»]
3CV2	X-ray	1.40	A/B	2-533	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P08997. 6 interactions.

Genome annotation databases

GeneID

948512.

GenomeReviews

Gene locus JW3974 in contig AP009048_GR.
Gene locus b4014 in contig U00096_GR.

KEGG

ecj:JW3974.
eco:b4014.

Organism-specific databases

EchoBASE

EB0022.

EcoGene

EG10023. aceB.

CMR

Search...

Phylogenomic databases

HOGENOM

P08997.

OMA

P08997. DYIFSCI.

Enzyme and pathway databases

BioCyc

EcoCyc:MALATE-SYNTHASE.
MetaCyc:MALATE-SYNTHASE.

Family and domain databases

InterPro

IPR011076. Malate_synth-like_core.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]

PANTHER

PTHR21631:SF1. Malate_synthase. 1 hit.

Pfam

PF01274. Malate_synthase. 1 hit.
[Graphical view]

PIRSF

PIRSF001363. Malate_synth. 1 hit.

TIGRFAMs

TIGR01344. malate_syn_A. 1 hit.

PROSITE

PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MASY_ECOLI

Accession

Primary (citable) accession number: P08997
Secondary accession number(s): Q2M6U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)