Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P08987

- GTFB_STRMU

UniProt

P08987 - GTFB_STRMU

Protein

Glucosyltransferase-I

Gene

gtfB

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 3 (06 Dec 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

    Catalytic activityi

    Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

    GO - Molecular functioni

    1. dextransucrase activity Source: UniProtKB-EC
    2. glucosyltransferase activity Source: InterPro

    GO - Biological processi

    1. glucan biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciSMUT210007:GC7Z-955-MONOMER.
    SABIO-RKP08987.

    Protein family/group databases

    CAZyiGH70. Glycoside Hydrolase Family 70.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosyltransferase-I (EC:2.4.1.5)
    Short name:
    GTF-I
    Alternative name(s):
    Dextransucrase
    Sucrose 6-glucosyltransferase
    Gene namesi
    Name:gtfB
    Ordered Locus Names:SMU_1004
    OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
    Taxonomic identifieri210007 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000002512: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Keywords - Diseasei

    Dental caries

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Sequence AnalysisAdd
    BLAST
    Chaini35 – 14761442Glucosyltransferase-IPRO_0000021385Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi210007.SMU.1004.

    Structurei

    3D structure databases

    ProteinModelPortaliP08987.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati159 – 17820Cell wall-binding 1Add
    BLAST
    Repeati179 – 19921Cell wall-binding 2Add
    BLAST
    Repeati1087 – 110620Cell wall-binding 3Add
    BLAST
    Repeati1107 – 112620Cell wall-binding 4Add
    BLAST
    Repeati1170 – 118920Cell wall-binding 5Add
    BLAST
    Repeati1214 – 123421Cell wall-binding 6Add
    BLAST
    Repeati1235 – 125420Cell wall-binding 7Add
    BLAST
    Repeati1279 – 129921Cell wall-binding 8Add
    BLAST
    Repeati1300 – 131920Cell wall-binding 9Add
    BLAST
    Repeati1344 – 136421Cell wall-binding 10Add
    BLAST
    Repeati1365 – 138420Cell wall-binding 11Add
    BLAST
    Repeati1409 – 142921Cell wall-binding 12Add
    BLAST
    Repeati1430 – 144920Cell wall-binding 13Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni200 – 1051852Catalytic; approximateAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 70 family.Curated
    Contains 13 cell wall-binding repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG5263.
    KOiK00689.
    OMAiANGVQVK.
    OrthoDBiEOG60PH6T.
    PhylomeDBiP08987.

    Family and domain databases

    Gene3Di3.20.20.80. 3 hits.
    InterProiIPR018337. Cell_wall/Cho-bd_repeat.
    IPR027636. Glucan-bd_rpt.
    IPR003318. Glyco_hydro70cat.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR022263. KxYKxGKxW.
    [Graphical view]
    PfamiPF01473. CW_binding_1. 4 hits.
    PF02324. Glyco_hydro_70. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 4 hits.
    TIGRFAMsiTIGR04035. glucan_65_rpt. 6 hits.
    TIGR03715. KxYKxGKxW. 1 hit.
    PROSITEiPS51170. CW. 13 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08987-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKKVRYKLR KVKKRWVTVS VASAVMTLTT LSGGLVKADS NESKSQISND     50
    SNTSVVTANE ESNVTTEVTS KQEAASSQTN HTVTTISSST SVVNPKEVVS 100
    NPYTVGETAS NGEKLQNQTT TVDKTSEAAA NNISKQTTEA DTDVIDDSNA 150
    ANLQILEKLP NVKEIDGKYY YYDNNGKVRT NFTLIADGKI LHFDETGAYT 200
    DTSIDTVNKD IVTTRSNLYK KYNQVYDRSA QSFEHVDHYL TAESWYRPKY 250
    ILKDGKTWTQ STEKDFRPLL MTWWPSQETQ RQYVNYMNAQ LGINKTYDDT 300
    SNQLQLNIAA ATIQAKIEAK ITTLKNTDWL RQTISAFVKT QSAWNSDSEK 350
    PFDDHLQNGA VLYDNEGKLT PYANSNYRIL NRTPTNQTGK KDPRYTADNT 400
    IGGYEFLLAN DVDNSNPVVQ AEQLNWLHFL MNFGNIYAND PDANFDSIRV 450
    DAVDNVDADL LQIAGDYLKA AKGIHKNDKA ANDHLSILEA WSDNDTPYLH 500
    DDGDNMINMD NKLRLSLLFS LAKPLNQRSG MNPLITNSLV NRTDDNAETA 550
    AVPSYSFIRA HDSEVQDLIR DIIKAEINPN VVGYSFTMEE IKKAFEIYNK 600
    DLLATEKKYT HYNTALSYAL LLTNKSSVPR VYYGDMFTDD GQYMAHKTIN 650
    YEAIETLLKA RIKYVSGGQA MRNQQVGNSE IITSVRYGKG ALKATDTGDR 700
    TTRTSGVAVI EGNNPSLRLK ASDRVVVNMG AAHKNQAYRP LLLTTDNGIK 750
    AYHSDQEAAG LVRYTNDRGE LIFTAADIKG YANPQVSGYL GVWVPVGAAA 800
    DQDVRVAAST APSTDGKSVH QNAALDSRVM FEGFSNFQAF ATKKEEYTNV 850
    VIAKNVDKFA EWGVTDFEMA PQYVSSTDGS FLDSVIQNGY AFTDRYDLGI 900
    SKPNKYGTAD DLVKAIKALH SKGIKVMADW VPDQMYAFPE KEVVTATRVD 950
    KFGKPVEGSQ IKSVLYVADS KSSGKDQQAK YGGAFLEELQ AKYPELFARK 1000
    QISTGVPMDP SVKIKQWSAK YFNGTNILGR GAGYVLKDQA TNTYFNISDN 1050
    KEINFLPKTL LNQDSQVGFS YDGKGYVYYS TSGYQAKNTF ISEGDKWYYF 1100
    DNNGYMVTGA QSINGVNYYF LSNGLQLRDA ILKNEDGTYA YYGNDGRRYE 1150
    NGYYQFMSGV WRHFNNGEMS VGLTVIDGQV QYFDEMGYQA KGKFVTTADG 1200
    KIRYFDKQSG NMYRNRFIEN EEGKWLYLGE DGAAVTGSQT INGQHLYFRA 1250
    NGVQVKGEFV TDRYGRISYY DSNSGDQIRN RFVRNAQGQW FYFDNNGYAV 1300
    TGARTINGQH LYFRANGVQV KGEFVTDRHG RISYYDGNSG DQIRNRFVRN 1350
    AQGQWFYFDN NGYAVTGART INGQHLYFRA NGVQVKGEFV TDRYGRISYY 1400
    DSNSGDQIRN RFVRNAQGQW FYFDNNGYAV TGARTINGQH LYFRANGVQV 1450
    KGEFVTDRYG RISYYDANSG ERVRIN 1476
    Length:1,476
    Mass (Da):165,847
    Last modified:December 6, 2002 - v3
    Checksum:i9C6E09F731B4CBCF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti570 – 5701R → A in AAA88588. (PubMed:3040685)Curated
    Sequence conflicti800 – 81718ADQDV…STDGK → LIKMFALRLARPHQQMA in AAA88588. (PubMed:3040685)CuratedAdd
    BLAST
    Sequence conflicti1310 – 13101H → L in AAA88588. (PubMed:3040685)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti62 – 621S → T in strain: MT4239.
    Natural varianti65 – 651T → I in strain: GS-5.
    Natural varianti68 – 681V → A in strain: GS-5, MT4245, MT4251, MT4467 and MT8148.
    Natural varianti78 – 781Q → P in strain: MT4251.
    Natural varianti86 – 861I → S in strain: GS-5, MT4245, MT4251, MT4467 and MT8148.
    Natural varianti89 – 891S → F in strain: MT4251.
    Natural varianti168 – 1681K → N in strain: MT4251.
    Natural varianti276 – 2761S → D in strain: GS-5, MT4467 and MT8148.
    Natural varianti399 – 3991N → R in strain: MT4239.
    Natural varianti474 – 4741I → T in strain: MT4239.
    Natural varianti512 – 5121K → R in strain: MT8148.
    Natural varianti519 – 5191F → Y in strain: MT8148.
    Natural varianti701 – 7011T → I in strain: MT8148.
    Natural varianti708 – 7081A → V in strain: MT8148.
    Natural varianti938 – 9381F → L in strain: MT8148.
    Natural varianti952 – 9576FGKPVE → YGTPVA in strain: GS-5, MT4239 and MT4467.
    Natural varianti963 – 9642SV → NT in strain: GS-5, MT4239 and MT4467.
    Natural varianti968 – 9703ADS → VDG in strain: GS-5, MT4239 and MT4467.
    Natural varianti1086 – 10861A → T in strain: MT4239.
    Natural varianti1158 – 11581S → N in strain: MT4239.
    Natural varianti1163 – 11631H → Y in strain: MT4251.
    Natural varianti1168 – 11681E → K in strain: MT8148.
    Natural varianti1182 – 11821Y → C in strain: MT8148.
    Natural varianti1234 – 12341A → P in strain: MT4239.
    Natural varianti1263 – 12631R → H in strain: GS-5 and MT4467.
    Natural varianti1263 – 12631R → P in strain: MT8148.
    Natural varianti1264 – 12641Y → H in strain: GS-5, MT4239, MT4467 and MT8148.
    Natural varianti1272 – 12721S → G in strain: GS-5, MT4239, MT4467 and MT8148.
    Natural varianti1329 – 13291H → Y in strain: GS-5 and MT4467.
    Natural varianti1394 – 13941Y → H in strain: GS-5, MT4239, MT4467 and MT8148.
    Natural varianti1402 – 14021S → G in strain: GS-5, MT4239, MT4467 and MT8148.
    Natural varianti1459 – 14591Y → H in strain: MT4467.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17361 Genomic DNA. Translation: AAA88588.1.
    D88651 Genomic DNA. Translation: BAA26101.1.
    D88654 Genomic DNA. Translation: BAA26105.1.
    D88657 Genomic DNA. Translation: BAA26109.1.
    D88660 Genomic DNA. Translation: BAA26113.1.
    D89977 Genomic DNA. Translation: BAA26119.1.
    AE014133 Genomic DNA. Translation: AAN58705.1.
    PIRiB33135.
    RefSeqiNP_721399.1. NC_004350.2.

    Genome annotation databases

    EnsemblBacteriaiAAN58705; AAN58705; SMU_1004.
    GeneIDi1028336.
    KEGGismu:SMU_1004.
    PATRICi19664129. VBIStrMut61772_0897.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17361 Genomic DNA. Translation: AAA88588.1 .
    D88651 Genomic DNA. Translation: BAA26101.1 .
    D88654 Genomic DNA. Translation: BAA26105.1 .
    D88657 Genomic DNA. Translation: BAA26109.1 .
    D88660 Genomic DNA. Translation: BAA26113.1 .
    D89977 Genomic DNA. Translation: BAA26119.1 .
    AE014133 Genomic DNA. Translation: AAN58705.1 .
    PIRi B33135.
    RefSeqi NP_721399.1. NC_004350.2.

    3D structure databases

    ProteinModelPortali P08987.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 210007.SMU.1004.

    Protein family/group databases

    CAZyi GH70. Glycoside Hydrolase Family 70.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN58705 ; AAN58705 ; SMU_1004 .
    GeneIDi 1028336.
    KEGGi smu:SMU_1004.
    PATRICi 19664129. VBIStrMut61772_0897.

    Phylogenomic databases

    eggNOGi COG5263.
    KOi K00689.
    OMAi ANGVQVK.
    OrthoDBi EOG60PH6T.
    PhylomeDBi P08987.

    Enzyme and pathway databases

    BioCyci SMUT210007:GC7Z-955-MONOMER.
    SABIO-RK P08987.

    Family and domain databases

    Gene3Di 3.20.20.80. 3 hits.
    InterProi IPR018337. Cell_wall/Cho-bd_repeat.
    IPR027636. Glucan-bd_rpt.
    IPR003318. Glyco_hydro70cat.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR022263. KxYKxGKxW.
    [Graphical view ]
    Pfami PF01473. CW_binding_1. 4 hits.
    PF02324. Glyco_hydro_70. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 4 hits.
    TIGRFAMsi TIGR04035. glucan_65_rpt. 6 hits.
    TIGR03715. KxYKxGKxW. 1 hit.
    PROSITEi PS51170. CW. 13 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the gtfB gene from Streptococcus mutans."
      Shiroza T., Ueda S., Kuramitsu H.K.
      J. Bacteriol. 169:4263-4270(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: GS-5.
    2. "Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans."
      Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S.
      FEMS Microbiol. Lett. 161:331-336(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700610 / UA159.

    Entry informationi

    Entry nameiGTFB_STRMU
    AccessioniPrimary (citable) accession number: P08987
    Secondary accession number(s): O69381
    , O69384, O69387, O69390, O69396
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: December 6, 2002
    Last modified: October 1, 2014
    This is version 127 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3