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Protein

Glucosyltransferase-I

Gene

gtfB

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

Catalytic activityi

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

GO - Molecular functioni

  1. dextransucrase activity Source: UniProtKB-EC
  2. glucosyltransferase activity Source: InterPro

GO - Biological processi

  1. glucan biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-955-MONOMER.
SABIO-RKP08987.

Protein family/group databases

CAZyiGH70. Glycoside Hydrolase Family 70.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosyltransferase-I (EC:2.4.1.5)
Short name:
GTF-I
Alternative name(s):
Dextransucrase
Sucrose 6-glucosyltransferase
Gene namesi
Name:gtfB
Ordered Locus Names:SMU_1004
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002512: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Keywords - Diseasei

Dental caries

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Sequence AnalysisAdd
BLAST
Chaini35 – 14761442Glucosyltransferase-IPRO_0000021385Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU.1004.

Structurei

3D structure databases

ProteinModelPortaliP08987.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati159 – 17820Cell wall-binding 1Add
BLAST
Repeati179 – 19921Cell wall-binding 2Add
BLAST
Repeati1087 – 110620Cell wall-binding 3Add
BLAST
Repeati1107 – 112620Cell wall-binding 4Add
BLAST
Repeati1170 – 118920Cell wall-binding 5Add
BLAST
Repeati1214 – 123421Cell wall-binding 6Add
BLAST
Repeati1235 – 125420Cell wall-binding 7Add
BLAST
Repeati1279 – 129921Cell wall-binding 8Add
BLAST
Repeati1300 – 131920Cell wall-binding 9Add
BLAST
Repeati1344 – 136421Cell wall-binding 10Add
BLAST
Repeati1365 – 138420Cell wall-binding 11Add
BLAST
Repeati1409 – 142921Cell wall-binding 12Add
BLAST
Repeati1430 – 144920Cell wall-binding 13Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni200 – 1051852Catalytic; approximateAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 70 family.Curated
Contains 13 cell wall-binding repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG5263.
KOiK00689.
OMAiANGVQVK.
OrthoDBiEOG60PH6T.
PhylomeDBiP08987.

Family and domain databases

Gene3Di3.20.20.80. 3 hits.
InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view]
PfamiPF01473. CW_binding_1. 4 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 4 hits.
TIGRFAMsiTIGR04035. glucan_65_rpt. 6 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEiPS51170. CW. 13 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08987-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKVRYKLR KVKKRWVTVS VASAVMTLTT LSGGLVKADS NESKSQISND
60 70 80 90 100
SNTSVVTANE ESNVTTEVTS KQEAASSQTN HTVTTISSST SVVNPKEVVS
110 120 130 140 150
NPYTVGETAS NGEKLQNQTT TVDKTSEAAA NNISKQTTEA DTDVIDDSNA
160 170 180 190 200
ANLQILEKLP NVKEIDGKYY YYDNNGKVRT NFTLIADGKI LHFDETGAYT
210 220 230 240 250
DTSIDTVNKD IVTTRSNLYK KYNQVYDRSA QSFEHVDHYL TAESWYRPKY
260 270 280 290 300
ILKDGKTWTQ STEKDFRPLL MTWWPSQETQ RQYVNYMNAQ LGINKTYDDT
310 320 330 340 350
SNQLQLNIAA ATIQAKIEAK ITTLKNTDWL RQTISAFVKT QSAWNSDSEK
360 370 380 390 400
PFDDHLQNGA VLYDNEGKLT PYANSNYRIL NRTPTNQTGK KDPRYTADNT
410 420 430 440 450
IGGYEFLLAN DVDNSNPVVQ AEQLNWLHFL MNFGNIYAND PDANFDSIRV
460 470 480 490 500
DAVDNVDADL LQIAGDYLKA AKGIHKNDKA ANDHLSILEA WSDNDTPYLH
510 520 530 540 550
DDGDNMINMD NKLRLSLLFS LAKPLNQRSG MNPLITNSLV NRTDDNAETA
560 570 580 590 600
AVPSYSFIRA HDSEVQDLIR DIIKAEINPN VVGYSFTMEE IKKAFEIYNK
610 620 630 640 650
DLLATEKKYT HYNTALSYAL LLTNKSSVPR VYYGDMFTDD GQYMAHKTIN
660 670 680 690 700
YEAIETLLKA RIKYVSGGQA MRNQQVGNSE IITSVRYGKG ALKATDTGDR
710 720 730 740 750
TTRTSGVAVI EGNNPSLRLK ASDRVVVNMG AAHKNQAYRP LLLTTDNGIK
760 770 780 790 800
AYHSDQEAAG LVRYTNDRGE LIFTAADIKG YANPQVSGYL GVWVPVGAAA
810 820 830 840 850
DQDVRVAAST APSTDGKSVH QNAALDSRVM FEGFSNFQAF ATKKEEYTNV
860 870 880 890 900
VIAKNVDKFA EWGVTDFEMA PQYVSSTDGS FLDSVIQNGY AFTDRYDLGI
910 920 930 940 950
SKPNKYGTAD DLVKAIKALH SKGIKVMADW VPDQMYAFPE KEVVTATRVD
960 970 980 990 1000
KFGKPVEGSQ IKSVLYVADS KSSGKDQQAK YGGAFLEELQ AKYPELFARK
1010 1020 1030 1040 1050
QISTGVPMDP SVKIKQWSAK YFNGTNILGR GAGYVLKDQA TNTYFNISDN
1060 1070 1080 1090 1100
KEINFLPKTL LNQDSQVGFS YDGKGYVYYS TSGYQAKNTF ISEGDKWYYF
1110 1120 1130 1140 1150
DNNGYMVTGA QSINGVNYYF LSNGLQLRDA ILKNEDGTYA YYGNDGRRYE
1160 1170 1180 1190 1200
NGYYQFMSGV WRHFNNGEMS VGLTVIDGQV QYFDEMGYQA KGKFVTTADG
1210 1220 1230 1240 1250
KIRYFDKQSG NMYRNRFIEN EEGKWLYLGE DGAAVTGSQT INGQHLYFRA
1260 1270 1280 1290 1300
NGVQVKGEFV TDRYGRISYY DSNSGDQIRN RFVRNAQGQW FYFDNNGYAV
1310 1320 1330 1340 1350
TGARTINGQH LYFRANGVQV KGEFVTDRHG RISYYDGNSG DQIRNRFVRN
1360 1370 1380 1390 1400
AQGQWFYFDN NGYAVTGART INGQHLYFRA NGVQVKGEFV TDRYGRISYY
1410 1420 1430 1440 1450
DSNSGDQIRN RFVRNAQGQW FYFDNNGYAV TGARTINGQH LYFRANGVQV
1460 1470
KGEFVTDRYG RISYYDANSG ERVRIN
Length:1,476
Mass (Da):165,847
Last modified:December 6, 2002 - v3
Checksum:i9C6E09F731B4CBCF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti570 – 5701R → A in AAA88588 (PubMed:3040685).Curated
Sequence conflicti800 – 81718ADQDV…STDGK → LIKMFALRLARPHQQMA in AAA88588 (PubMed:3040685).CuratedAdd
BLAST
Sequence conflicti1310 – 13101H → L in AAA88588 (PubMed:3040685).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti62 – 621S → T in strain: MT4239.
Natural varianti65 – 651T → I in strain: GS-5.
Natural varianti68 – 681V → A in strain: GS-5, MT4245, MT4251, MT4467 and MT8148.
Natural varianti78 – 781Q → P in strain: MT4251.
Natural varianti86 – 861I → S in strain: GS-5, MT4245, MT4251, MT4467 and MT8148.
Natural varianti89 – 891S → F in strain: MT4251.
Natural varianti168 – 1681K → N in strain: MT4251.
Natural varianti276 – 2761S → D in strain: GS-5, MT4467 and MT8148.
Natural varianti399 – 3991N → R in strain: MT4239.
Natural varianti474 – 4741I → T in strain: MT4239.
Natural varianti512 – 5121K → R in strain: MT8148.
Natural varianti519 – 5191F → Y in strain: MT8148.
Natural varianti701 – 7011T → I in strain: MT8148.
Natural varianti708 – 7081A → V in strain: MT8148.
Natural varianti938 – 9381F → L in strain: MT8148.
Natural varianti952 – 9576FGKPVE → YGTPVA in strain: GS-5, MT4239 and MT4467.
Natural varianti963 – 9642SV → NT in strain: GS-5, MT4239 and MT4467.
Natural varianti968 – 9703ADS → VDG in strain: GS-5, MT4239 and MT4467.
Natural varianti1086 – 10861A → T in strain: MT4239.
Natural varianti1158 – 11581S → N in strain: MT4239.
Natural varianti1163 – 11631H → Y in strain: MT4251.
Natural varianti1168 – 11681E → K in strain: MT8148.
Natural varianti1182 – 11821Y → C in strain: MT8148.
Natural varianti1234 – 12341A → P in strain: MT4239.
Natural varianti1263 – 12631R → H in strain: GS-5 and MT4467.
Natural varianti1263 – 12631R → P in strain: MT8148.
Natural varianti1264 – 12641Y → H in strain: GS-5, MT4239, MT4467 and MT8148.
Natural varianti1272 – 12721S → G in strain: GS-5, MT4239, MT4467 and MT8148.
Natural varianti1329 – 13291H → Y in strain: GS-5 and MT4467.
Natural varianti1394 – 13941Y → H in strain: GS-5, MT4239, MT4467 and MT8148.
Natural varianti1402 – 14021S → G in strain: GS-5, MT4239, MT4467 and MT8148.
Natural varianti1459 – 14591Y → H in strain: MT4467.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17361 Genomic DNA. Translation: AAA88588.1.
D88651 Genomic DNA. Translation: BAA26101.1.
D88654 Genomic DNA. Translation: BAA26105.1.
D88657 Genomic DNA. Translation: BAA26109.1.
D88660 Genomic DNA. Translation: BAA26113.1.
D89977 Genomic DNA. Translation: BAA26119.1.
AE014133 Genomic DNA. Translation: AAN58705.1.
PIRiB33135.
RefSeqiNP_721399.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58705; AAN58705; SMU_1004.
GeneIDi1028336.
KEGGismu:SMU_1004.
PATRICi19664129. VBIStrMut61772_0897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17361 Genomic DNA. Translation: AAA88588.1.
D88651 Genomic DNA. Translation: BAA26101.1.
D88654 Genomic DNA. Translation: BAA26105.1.
D88657 Genomic DNA. Translation: BAA26109.1.
D88660 Genomic DNA. Translation: BAA26113.1.
D89977 Genomic DNA. Translation: BAA26119.1.
AE014133 Genomic DNA. Translation: AAN58705.1.
PIRiB33135.
RefSeqiNP_721399.1. NC_004350.2.

3D structure databases

ProteinModelPortaliP08987.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi210007.SMU.1004.

Protein family/group databases

CAZyiGH70. Glycoside Hydrolase Family 70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN58705; AAN58705; SMU_1004.
GeneIDi1028336.
KEGGismu:SMU_1004.
PATRICi19664129. VBIStrMut61772_0897.

Phylogenomic databases

eggNOGiCOG5263.
KOiK00689.
OMAiANGVQVK.
OrthoDBiEOG60PH6T.
PhylomeDBiP08987.

Enzyme and pathway databases

BioCyciSMUT210007:GC7Z-955-MONOMER.
SABIO-RKP08987.

Family and domain databases

Gene3Di3.20.20.80. 3 hits.
InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR027636. Glucan-bd_rpt.
IPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR022263. KxYKxGKxW.
[Graphical view]
PfamiPF01473. CW_binding_1. 4 hits.
PF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 4 hits.
TIGRFAMsiTIGR04035. glucan_65_rpt. 6 hits.
TIGR03715. KxYKxGKxW. 1 hit.
PROSITEiPS51170. CW. 13 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the gtfB gene from Streptococcus mutans."
    Shiroza T., Ueda S., Kuramitsu H.K.
    J. Bacteriol. 169:4263-4270(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GS-5.
  2. "Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans."
    Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S.
    FEMS Microbiol. Lett. 161:331-336(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700610 / UA159.

Entry informationi

Entry nameiGTFB_STRMU
AccessioniPrimary (citable) accession number: P08987
Secondary accession number(s): O69381
, O69384, O69387, O69390, O69396
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: December 6, 2002
Last modified: March 4, 2015
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.