Reviewed, UniProtKB/Swiss-Prot P08987 (GTFB_STRMU)

Names and origin

Protein names

Recommended name:
    Glucosyltransferase-I
      Short name=GTF-I
    EC=2.4.1.5
Alternative name(s):
    Dextransucrase
    Sucrose 6-glucosyltransferase

Gene names

Name:	gtfB
Ordered Locus Names:	SMU_1004

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	1476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
Catalytic activity	Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).
Subcellular location	Secreted.
Miscellaneous	GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 70 family. Contains 13 cell wall-binding repeats.

Ontologies

Keywords
Cellular component	Secreted
Disease	Dental caries
Domain	Repeat Signal
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucan biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro dextransucrase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 1476	1442	Glucosyltransferase-I		PRO_0000021385
Regions
	Repeat	159 – 178	20	Cell wall-binding 1
	Repeat	179 – 199	21	Cell wall-binding 2
	Repeat	1087 – 1106	20	Cell wall-binding 3
	Repeat	1107 – 1126	20	Cell wall-binding 4
	Repeat	1170 – 1189	20	Cell wall-binding 5
	Repeat	1214 – 1234	21	Cell wall-binding 6
	Repeat	1235 – 1254	20	Cell wall-binding 7
	Repeat	1279 – 1299	21	Cell wall-binding 8
	Repeat	1300 – 1319	20	Cell wall-binding 9
	Repeat	1344 – 1364	21	Cell wall-binding 10
	Repeat	1365 – 1384	20	Cell wall-binding 11
	Repeat	1409 – 1429	21	Cell wall-binding 12
	Repeat	1430 – 1449	20	Cell wall-binding 13
	Region	200 – 1051	852	Catalytic; approximate
Natural variations
	Natural variant	62	1	S → T in strain: MT4239.
	Natural variant	65	1	T → I in strain: GS-5.
	Natural variant	68	1	V → A in strain: GS-5, MT4245, MT4251, MT4467 and MT8148.
	Natural variant	78	1	Q → P in strain: MT4251.
	Natural variant	86	1	I → S in strain: GS-5, MT4245, MT4251, MT4467 and MT8148.
	Natural variant	89	1	S → F in strain: MT4251.
	Natural variant	168	1	K → N in strain: MT4251.
	Natural variant	276	1	S → D in strain: GS-5, MT4467 and MT8148.
	Natural variant	399	1	N → R in strain: MT4239.
	Natural variant	474	1	I → T in strain: MT4239.
	Natural variant	512	1	K → R in strain: MT8148.
	Natural variant	519	1	F → Y in strain: MT8148.
	Natural variant	701	1	T → I in strain: MT8148.
	Natural variant	708	1	A → V in strain: MT8148.
	Natural variant	938	1	F → L in strain: MT8148.
	Natural variant	952 – 957	6	FGKPVE → YGTPVA in strain: GS-5, MT4239 and MT4467.
	Natural variant	963 – 964	2	SV → NT in strain: GS-5, MT4239 and MT4467.
	Natural variant	968 – 970	3	ADS → VDG in strain: GS-5, MT4239 and MT4467.
	Natural variant	1086	1	A → T in strain: MT4239.
	Natural variant	1158	1	S → N in strain: MT4239.
	Natural variant	1163	1	H → Y in strain: MT4251.
	Natural variant	1168	1	E → K in strain: MT8148.
	Natural variant	1182	1	Y → C in strain: MT8148.
	Natural variant	1234	1	A → P in strain: MT4239.
	Natural variant	1263	1	R → H in strain: GS-5 and MT4467.
	Natural variant	1263	1	R → P in strain: MT8148.
	Natural variant	1264	1	Y → H in strain: GS-5, MT4239, MT4467 and MT8148.
	Natural variant	1272	1	S → G in strain: GS-5, MT4239, MT4467 and MT8148.
	Natural variant	1329	1	H → Y in strain: GS-5 and MT4467.
	Natural variant	1394	1	Y → H in strain: GS-5, MT4239, MT4467 and MT8148.
	Natural variant	1402	1	S → G in strain: GS-5, MT4239, MT4467 and MT8148.
	Natural variant	1459	1	Y → H in strain: MT4467.
Experimental info
	Sequence conflict	570	1	R → A in AAA88588. Ref.1
	Sequence conflict	800 – 817	18	ADQDV…STDGK → LIKMFALRLARPHQQMA in AAA88588. Ref.1
	Sequence conflict	1310	1	H → L in AAA88588. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P08987-1 [UniParc]. Last modified December 6, 2002. Version 3. Checksum: 9C6E09F731B4CBCF	FASTA	1,476	165,847
	10 20 30 40 50 60 MDKKVRYKLR KVKKRWVTVS VASAVMTLTT LSGGLVKADS NESKSQISND SNTSVVTANE 70 80 90 100 110 120 ESNVTTEVTS KQEAASSQTN HTVTTISSST SVVNPKEVVS NPYTVGETAS NGEKLQNQTT 130 140 150 160 170 180 TVDKTSEAAA NNISKQTTEA DTDVIDDSNA ANLQILEKLP NVKEIDGKYY YYDNNGKVRT 190 200 210 220 230 240 NFTLIADGKI LHFDETGAYT DTSIDTVNKD IVTTRSNLYK KYNQVYDRSA QSFEHVDHYL 250 260 270 280 290 300 TAESWYRPKY ILKDGKTWTQ STEKDFRPLL MTWWPSQETQ RQYVNYMNAQ LGINKTYDDT 310 320 330 340 350 360 SNQLQLNIAA ATIQAKIEAK ITTLKNTDWL RQTISAFVKT QSAWNSDSEK PFDDHLQNGA 370 380 390 400 410 420 VLYDNEGKLT PYANSNYRIL NRTPTNQTGK KDPRYTADNT IGGYEFLLAN DVDNSNPVVQ 430 440 450 460 470 480 AEQLNWLHFL MNFGNIYAND PDANFDSIRV DAVDNVDADL LQIAGDYLKA AKGIHKNDKA 490 500 510 520 530 540 ANDHLSILEA WSDNDTPYLH DDGDNMINMD NKLRLSLLFS LAKPLNQRSG MNPLITNSLV 550 560 570 580 590 600 NRTDDNAETA AVPSYSFIRA HDSEVQDLIR DIIKAEINPN VVGYSFTMEE IKKAFEIYNK 610 620 630 640 650 660 DLLATEKKYT HYNTALSYAL LLTNKSSVPR VYYGDMFTDD GQYMAHKTIN YEAIETLLKA 670 680 690 700 710 720 RIKYVSGGQA MRNQQVGNSE IITSVRYGKG ALKATDTGDR TTRTSGVAVI EGNNPSLRLK 730 740 750 760 770 780 ASDRVVVNMG AAHKNQAYRP LLLTTDNGIK AYHSDQEAAG LVRYTNDRGE LIFTAADIKG 790 800 810 820 830 840 YANPQVSGYL GVWVPVGAAA DQDVRVAAST APSTDGKSVH QNAALDSRVM FEGFSNFQAF 850 860 870 880 890 900 ATKKEEYTNV VIAKNVDKFA EWGVTDFEMA PQYVSSTDGS FLDSVIQNGY AFTDRYDLGI 910 920 930 940 950 960 SKPNKYGTAD DLVKAIKALH SKGIKVMADW VPDQMYAFPE KEVVTATRVD KFGKPVEGSQ 970 980 990 1000 1010 1020 IKSVLYVADS KSSGKDQQAK YGGAFLEELQ AKYPELFARK QISTGVPMDP SVKIKQWSAK 1030 1040 1050 1060 1070 1080 YFNGTNILGR GAGYVLKDQA TNTYFNISDN KEINFLPKTL LNQDSQVGFS YDGKGYVYYS 1090 1100 1110 1120 1130 1140 TSGYQAKNTF ISEGDKWYYF DNNGYMVTGA QSINGVNYYF LSNGLQLRDA ILKNEDGTYA 1150 1160 1170 1180 1190 1200 YYGNDGRRYE NGYYQFMSGV WRHFNNGEMS VGLTVIDGQV QYFDEMGYQA KGKFVTTADG 1210 1220 1230 1240 1250 1260 KIRYFDKQSG NMYRNRFIEN EEGKWLYLGE DGAAVTGSQT INGQHLYFRA NGVQVKGEFV 1270 1280 1290 1300 1310 1320 TDRYGRISYY DSNSGDQIRN RFVRNAQGQW FYFDNNGYAV TGARTINGQH LYFRANGVQV 1330 1340 1350 1360 1370 1380 KGEFVTDRHG RISYYDGNSG DQIRNRFVRN AQGQWFYFDN NGYAVTGART INGQHLYFRA 1390 1400 1410 1420 1430 1440 NGVQVKGEFV TDRYGRISYY DSNSGDQIRN RFVRNAQGQW FYFDNNGYAV TGARTINGQH 1450 1460 1470 LYFRANGVQV KGEFVTDRYG RISYYDANSG ERVRIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the gtfB gene from Streptococcus mutans." Shiroza T., Ueda S., Kuramitsu H.K. J. Bacteriol. 169:4263-4270(1987) [PubMed: 3040685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GS-5.
[2]	"Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans." Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S. FEMS Microbiol. Lett. 161:331-336(1998) [PubMed: 9570124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
[3]	"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17361 Genomic DNA. Translation: AAA88588.1. D88651 Genomic DNA. Translation: BAA26101.1. D88654 Genomic DNA. Translation: BAA26105.1. D88657 Genomic DNA. Translation: BAA26109.1. D88660 Genomic DNA. Translation: BAA26113.1. D89977 Genomic DNA. Translation: BAA26119.1. AE014133 Genomic DNA. Translation: AAN58705.1.
PIR	B33135.
RefSeq	NP_721399.1.
3D structure databases
HSSP	HSSP built from PDB template 1H8G based on UniProtKB P06653.
ModBase	Search...
Protein family/group databases
CAZy	GH70. Glycoside Hydrolase Family 70.
Genome annotation databases
GeneID	1028336.
GenomeReviews	Gene locus SMU_1004 in contig AE014133_GR.
KEGG	smu:SMU.1004.
NMPDR	fig|210007.1.peg.912.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P08987.
OMA	P08987. LYFRANG.
Enzyme and pathway databases
BioCyc	SMUT210007:SMU_1004-MON.
BRENDA	2.4.1.5. 20716.
Family and domain databases
InterPro	IPR018337. Cell_wall/Cho-bd_repeat. IPR002479. Cell_wall_bd_put. IPR003318. Glyco_hydro70cat. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
Pfam	PF01473. CW_binding_1. 4 hits. PF02324. Glyco_hydro_70. 1 hit. [Graphical view]
PROSITE	PS51170. CW. 13 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GTFB_STRMU

Accession

Primary (citable) accession number: P08987
Secondary accession number(s): O69381 , O69384, O69387, O69390, O69396

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	December 6, 2002
Last modified:	June 16, 2009
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)