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Protein

Histone H2A.v

Gene

His2Av

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acts as a Polycomb group (PcG) protein required to maintain the transcriptionally repressive state of homeotic genes of the animal throughout development. Required for histone H3 'Lys-9' methylation and histone H4 'Lys-12' acetylation, two modifications that are essential for heterochromatin formation. Also involved in DNA double strand break (DSB) repair. Essential for early development.4 Publications

GO - Molecular functioni

GO - Biological processi

  • chromatin silencing Source: GO_Central
  • DNA repair Source: UniProtKB-KW
  • male germ-line stem cell population maintenance Source: FlyBase
  • multicellular organism development Source: UniProtKB-KW
  • protein localization to chromatin Source: FlyBase
  • somatic stem cell population maintenance Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-DME-201722 Formation of the beta-catenin:TCF transactivating complex
R-DME-212300 PRC2 methylates histones and DNA
R-DME-2299718 Condensation of Prophase Chromosomes
R-DME-2559580 Oxidative Stress Induced Senescence
R-DME-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-DME-427359 SIRT1 negatively regulates rRNA expression
R-DME-427413 NoRC negatively regulates rRNA expression
R-DME-5578749 Transcriptional regulation by small RNAs
R-DME-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-DME-73777 RNA Polymerase I Chain Elongation
R-DME-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-DME-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2A.v
Alternative name(s):
H2A.F/Z
Short name:
H2A.Z
Gene namesi
Name:His2Av
Synonyms:H2AvD, His2AvD
ORF Names:CG5499
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0001197 His2Av

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000553092 – 141Histone H2A.vAdd BLAST140

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5N6-acetyllysine1 Publication1
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei138Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated. Phosphorylation of Ser-138 occurs in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents. Phosphorylation is dependent on the DNA damage checkpoint kinases ATR and ATM, spreads on either side of a detected DSB site and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair.1 Publication
Acetylated on Lys-5 by Tip60. Acetylation is enhanced by Ser-138 phosphorylation and promotes the exchange of the phosphorylated form with the unmodified form of H2AV.2 Publications
Monoubiquitination of Lys-121 by sce/dRING gives a specific tag for epigenetic transcriptional repression.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP08985
PRIDEiP08985

PTM databases

iPTMnetiP08985

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Expressed in embryos and adults (females only).1 Publication

Gene expression databases

BgeeiFBgn0001197
ExpressionAtlasiP08985 baseline and differential
GenevisibleiP08985 DM

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant His2Av forms a heterodimer with H2B.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi68120, 24 interactors
DIPiDIP-23205N
IntActiP08985, 2 interactors
MINTiP08985
STRINGi7227.FBpp0084434

Structurei

3D structure databases

ProteinModelPortaliP08985
SMRiP08985
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 103Essential for function in developmentAdd BLAST11

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi138 – 139[ST]-Q motif2

Domaini

The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.

Sequence similaritiesi

Belongs to the histone H2A family.Curated

Phylogenomic databases

eggNOGiKOG1757 Eukaryota
COG5262 LUCA
GeneTreeiENSGT00900000140979
InParanoidiP08985
KOiK11251
OMAiKQRTQHN
OrthoDBiEOG091G0XGD
PhylomeDBiP08985

Family and domain databases

CDDicd00074 H2A, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR002119 Histone_H2A
IPR007125 Histone_H2A/H2B/H3
IPR032454 Histone_H2A_C
IPR032458 Histone_H2A_CS
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PF16211 Histone_H2A_C, 1 hit
PRINTSiPR00620 HISTONEH2A
SMARTiView protein in SMART
SM00414 H2A, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00046 HISTONE_H2A, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08985-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGKAGKDS GKAKAKAVSR SARAGLQFPV GRIHRHLKSR TTSHGRVGAT
60 70 80 90 100
AAVYSAAILE YLTAEVLELA GNASKDLKVK RITPRHLQLA IRGDEELDSL
110 120 130 140
IKATIAGGGV IPHIHKSLIG KKEETVQDPQ RKGNVILSQA Y
Length:141
Mass (Da):14,981
Last modified:January 23, 2007 - v2
Checksum:i0DA41C01F831A5B6
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti25G → D in strain: ZBMEL377. 1
Natural varianti90A → T in strain: ZBMEL131. 1
Natural varianti95E → K in strain: MEL16 and ZBMEL84. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07485 mRNA Translation: CAA30370.1
X15549 Genomic DNA Translation: CAA33555.1
AM294365 Genomic DNA Translation: CAL26295.1
AM294366 Genomic DNA Translation: CAL26296.1
AM294367 Genomic DNA Translation: CAL26297.1
AM294368 Genomic DNA Translation: CAL26298.1
AM294369 Genomic DNA Translation: CAL26299.1
AM294370 Genomic DNA Translation: CAL26300.1
AM294371 Genomic DNA Translation: CAL26301.1
AM294372 Genomic DNA Translation: CAL26302.1
FM245434 Genomic DNA Translation: CAR93360.1
FM245435 Genomic DNA Translation: CAR93361.1
FM245436 Genomic DNA Translation: CAR93362.1
FM245437 Genomic DNA Translation: CAR93363.1
FM245438 Genomic DNA Translation: CAR93364.1
FM245439 Genomic DNA Translation: CAR93365.1
FM245440 Genomic DNA Translation: CAR93366.1
FM245441 Genomic DNA Translation: CAR93367.1
FM245442 Genomic DNA Translation: CAR93368.1
FM245443 Genomic DNA Translation: CAR93369.1
FM245444 Genomic DNA Translation: CAR93370.1
FM245445 Genomic DNA Translation: CAR93371.1
AE014297 Genomic DNA Translation: AAF56631.1
AY118910 mRNA Translation: AAM50770.1
S42733 Genomic DNA Translation: AAB22897.1
PIRiS08118
RefSeqiNP_001262997.1, NM_001276068.1
NP_524519.1, NM_079795.3
UniGeneiDm.2346

Genome annotation databases

EnsemblMetazoaiFBtr0085062; FBpp0084434; FBgn0001197
FBtr0334311; FBpp0306426; FBgn0001197
GeneIDi43229
KEGGidme:Dmel_CG5499

Similar proteinsi

Entry informationi

Entry nameiH2AV_DROME
AccessioniPrimary (citable) accession number: P08985
Secondary accession number(s): A0ANX7
, A0ANX8, A0ANY0, A0ANY3, C0MJB2, C0MJB9, Q26252, Q540X2, Q9VBB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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