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P08980 (UCRIA_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
EC=1.10.99.1
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Rieske iron-sulfur protein
Short name=ISP
Short name=RISP
Gene names
Name:petC
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.

Catalytic activity

Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.

Cofactor

Binds 1 2Fe-2S cluster per subunit.

Subunit structure

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.

Subcellular location

Plastid › chloroplast thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.

Miscellaneous

This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.

The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Sequence similarities

Contains 1 Rieske domain.

Sequence caution

The sequence CAA29590.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5151Chloroplast Ref.2
Chain52 – 230179Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
PRO_0000030693

Regions

Transmembrane73 – 9321Helical; Probable
Domain116 – 21297Rieske

Sites

Metal binding1581Iron-sulfur (2Fe-2S) Ref.3
Metal binding1601Iron-sulfur (2Fe-2S); via pros nitrogen
Metal binding1761Iron-sulfur (2Fe-2S) Ref.3
Metal binding1791Iron-sulfur (2Fe-2S); via pros nitrogen

Amino acid modifications

Disulfide bond163 ↔ 178 Ref.3

Secondary structure

1 .............................. 230
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08980 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: FA2C54B4C6586059

FASTA23024,325
        10         20         30         40         50         60 
MASFTLSSAT PSQLCSSKNG MFAPSLALAK AGRVNVLISK ERIRGMKLTC QATSIPADNV 

        70         80         90        100        110        120 
PDMQKRETLN LLLLGALSLP TGYMLLPYAS FFVPPGGGAG TGGTIAKDAL GNDVIAAEWL 

       130        140        150        160        170        180 
KTHAPGDRTL TQGLKGDPTY LVVESDKTLA TFGINAVCTH LGCVVPFNAA ENKFICPCHG 

       190        200        210        220        230 
SQYNNQGRVV RGPAPLSLAL AHCDVDDGKV VFVPWTETDF RTGEAPWWSA

« Hide

References

[1]"The complete amino-acid sequence of the Rieske FeS-precursor protein from spinach chloroplasts deduced from cDNA analysis."
Steppuhn J., Rother C., Hermans J., Jansen T., Salnikow J., Hauska G., Herrmann R.G.
Mol. Gen. Genet. 210:171-177(1987)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Monatol.
[2]"N-terminal amino acid sequence of the Rieske iron-sulfur protein from the cytochrome b6/f-complex of spinach thylakoids."
Pfefferkorn B., Meyer H.E.
FEBS Lett. 206:233-237(1986)
Cited for: PROTEIN SEQUENCE OF 52-147.
[3]"Biological identity and diversity in photosynthesis and respiration: structure of the lumen-side domain of the chloroplast Rieske protein."
Carrell C.J., Zhang H., Cramer W.A., Smith J.L.
Structure 5:1613-1625(1997) [PubMed: 9438861] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF 104-230.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X06244 mRNA. Translation: CAA29590.1. Different initiation.
PIRS00454.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RFSX-ray1.83A92-230[»]
ProteinModelPortalP08980.
SMRP08980. Positions 59-230.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
Gene3DG3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHERPTHR10134. Rieske. 1 hit.
PfamPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSPR00162. RIESKE.
SUPFAMSSF50022. Rieske_dom. 1 hit.
PROSITEPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUCRIA_SPIOL
AccessionPrimary (citable) accession number: P08980
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: June 21, 2005
Last modified: October 19, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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