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Protein

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

Gene

petC

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.By similarity

Catalytic activityi

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

Cofactori

[2Fe-2S] cluster1 PublicationNote: Binds 1 [2Fe-2S] cluster per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi158Iron-sulfur (2Fe-2S)1 Publication1
Metal bindingi160Iron-sulfur (2Fe-2S); via pros nitrogen1 Publication1
Metal bindingi176Iron-sulfur (2Fe-2S)1 Publication1
Metal bindingi179Iron-sulfur (2Fe-2S); via pros nitrogen1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (EC:1.10.9.1)
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Rieske iron-sulfur protein
Short name:
ISP
Short name:
RISP
Gene namesi
Name:petC
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

  • Plastidchloroplast thylakoid membrane 1 Publication; Single-pass membrane protein 1 Publication

  • Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei73 – 93HelicalCuratedAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 51Chloroplast1 PublicationAdd BLAST51
ChainiPRO_000003069352 – 230Cytochrome b6-f complex iron-sulfur subunit, chloroplasticAdd BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi163 ↔ 1781 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP08980.

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer (By similarity).By similarity

Structurei

Secondary structure

1230
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi116 – 122Combined sources7
Beta strandi128 – 132Combined sources5
Helixi134 – 136Combined sources3
Beta strandi138 – 143Combined sources6
Beta strandi147 – 149Combined sources3
Beta strandi151 – 155Combined sources5
Turni159 – 161Combined sources3
Turni169 – 172Combined sources4
Beta strandi173 – 175Combined sources3
Turni177 – 179Combined sources3
Beta strandi182 – 184Combined sources3
Beta strandi189 – 193Combined sources5
Beta strandi200 – 206Combined sources7
Beta strandi209 – 214Combined sources6
Turni220 – 222Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RFSX-ray1.83A92-230[»]
ProteinModelPortaliP08980.
SMRiP08980.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08980.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 212RieskeAdd BLAST97

Sequence similaritiesi

Contains 1 Rieske domain.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01335. Cytb6_f_Rieske. 1 hit.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFTLSSAT PSQLCSSKNG MFAPSLALAK AGRVNVLISK ERIRGMKLTC
60 70 80 90 100
QATSIPADNV PDMQKRETLN LLLLGALSLP TGYMLLPYAS FFVPPGGGAG
110 120 130 140 150
TGGTIAKDAL GNDVIAAEWL KTHAPGDRTL TQGLKGDPTY LVVESDKTLA
160 170 180 190 200
TFGINAVCTH LGCVVPFNAA ENKFICPCHG SQYNNQGRVV RGPAPLSLAL
210 220 230
AHCDVDDGKV VFVPWTETDF RTGEAPWWSA
Length:230
Mass (Da):24,325
Last modified:June 21, 2005 - v2
Checksum:iFA2C54B4C6586059
GO

Sequence cautioni

The sequence CAA29590 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06244 mRNA. Translation: CAA29590.1. Different initiation.
PIRiS00454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06244 mRNA. Translation: CAA29590.1. Different initiation.
PIRiS00454.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RFSX-ray1.83A92-230[»]
ProteinModelPortaliP08980.
SMRiP08980.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP08980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP08980.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01335. Cytb6_f_Rieske. 1 hit.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUCRIA_SPIOL
AccessioniPrimary (citable) accession number: P08980
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: June 21, 2005
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.
The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.