Reviewed, UniProtKB/Swiss-Prot P08980 (UCRIA_SPIOL)

Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP
      Short name=ISP
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein

Gene names

Name:

petC

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.
Cofactor	Binds 1 2Fe-2S cluster per subunit.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.
Miscellaneous	This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus. The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 51

51

Chloroplast Ref.2

Chain

52 – 230

179

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

PRO_0000030693

Regions

Transmembrane

73 – 93

21

Probable

Domain

116 – 212

97

Rieske

Sites

Metal binding

158

1

Iron-sulfur (2Fe-2S) Ref.3

Metal binding

160

1

Iron-sulfur (2Fe-2S); via pros nitrogen

Metal binding

176

1

Iron-sulfur (2Fe-2S) Ref.3

Metal binding

179

1

Iron-sulfur (2Fe-2S); via pros nitrogen

Amino acid modifications

Disulfide bond

163 ↔ 178

Ref.3

Secondary structure

1

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230

Helix Strand Turn

Details...

Helix

116 – 122

7

Beta strand

128 – 132

5

Helix

134 – 136

3

Beta strand

138 – 143

6

Beta strand

147 – 149

3

Beta strand

151 – 155

5

Turn

159 – 161

3

Turn

169 – 172

4

Beta strand

173 – 175

3

Turn

177 – 179

3

Beta strand

182 – 184

3

Beta strand

189 – 193

5

Beta strand

200 – 206

7

Beta strand

209 – 214

6

Turn

220 – 222

3

Sequences

	Sequence		Length	Mass (Da)
	P08980-1 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: FA2C54B4C6586059	FASTA	230	24,325
	10 20 30 40 50 60 MASFTLSSAT PSQLCSSKNG MFAPSLALAK AGRVNVLISK ERIRGMKLTC QATSIPADNV 70 80 90 100 110 120 PDMQKRETLN LLLLGALSLP TGYMLLPYAS FFVPPGGGAG TGGTIAKDAL GNDVIAAEWL 130 140 150 160 170 180 KTHAPGDRTL TQGLKGDPTY LVVESDKTLA TFGINAVCTH LGCVVPFNAA ENKFICPCHG 190 200 210 220 230 SQYNNQGRVV RGPAPLSLAL AHCDVDDGKV VFVPWTETDF RTGEAPWWSA

References

[1]	"The complete amino-acid sequence of the Rieske FeS-precursor protein from spinach chloroplasts deduced from cDNA analysis." Steppuhn J., Rother C., Hermans J., Jansen T., Salnikow J., Hauska G., Herrmann R.G. Mol. Gen. Genet. 210:171-177(1987) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Monatol.
[2]	"N-terminal amino acid sequence of the Rieske iron-sulfur protein from the cytochrome b6/f-complex of spinach thylakoids." Pfefferkorn B., Meyer H.E. FEBS Lett. 206:233-237(1986) Cited for: PROTEIN SEQUENCE OF 52-147.
[3]	"Biological identity and diversity in photosynthesis and respiration: structure of the lumen-side domain of the chloroplast Rieske protein." Carrell C.J., Zhang H., Cramer W.A., Smith J.L. Structure 5:1613-1625(1997) [PubMed: 9438861] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF 104-230.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X06244 mRNA. Translation: CAA29590.1. Different initiation.

PIR

S00454.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RFS	X-ray	1.83	A	92-230	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.10.99.1. 286.

Family and domain databases

InterPro

IPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
[Graphical view]

Gene3D

G3DSA:2.102.10.10. Rieske_reg. 1 hit.

PANTHER

PTHR10134. Rieske. 1 hit.

Pfam

PF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]

PROSITE

PS51296. RIESKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

UCRIA_SPIOL

Accession

Primary (citable) accession number: P08980

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	June 21, 2005
Last modified:	June 16, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)