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Protein

Cytochrome b6-f complex iron-sulfur subunit, chloroplastic

Gene

petC

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.By similarity

Catalytic activityi

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H+(Side 2).

Cofactori

[2Fe-2S] cluster1 PublicationNote: Binds 1 [2Fe-2S] cluster per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi158 – 1581Iron-sulfur (2Fe-2S)1 Publication
Metal bindingi160 – 1601Iron-sulfur (2Fe-2S); via pros nitrogen1 Publication
Metal bindingi176 – 1761Iron-sulfur (2Fe-2S)1 Publication
Metal bindingi179 – 1791Iron-sulfur (2Fe-2S); via pros nitrogen1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (EC:1.10.9.1)
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Rieske iron-sulfur protein
Short name:
ISP
Short name:
RISP
Gene namesi
Name:petC
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

  • Plastidchloroplast thylakoid membrane 1 Publication; Single-pass membrane protein 1 Publication

  • Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei73 – 9321HelicalCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5151Chloroplast1 PublicationAdd
BLAST
Chaini52 – 230179Cytochrome b6-f complex iron-sulfur subunit, chloroplasticPRO_0000030693Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi163 ↔ 1781 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer (By similarity).By similarity

Structurei

Secondary structure

1
230
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi116 – 1227Combined sources
Beta strandi128 – 1325Combined sources
Helixi134 – 1363Combined sources
Beta strandi138 – 1436Combined sources
Beta strandi147 – 1493Combined sources
Beta strandi151 – 1555Combined sources
Turni159 – 1613Combined sources
Turni169 – 1724Combined sources
Beta strandi173 – 1753Combined sources
Turni177 – 1793Combined sources
Beta strandi182 – 1843Combined sources
Beta strandi189 – 1935Combined sources
Beta strandi200 – 2067Combined sources
Beta strandi209 – 2146Combined sources
Turni220 – 2223Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RFSX-ray1.83A92-230[»]
ProteinModelPortaliP08980.
SMRiP08980. Positions 59-230.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08980.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini116 – 21297RieskeAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01335. Cytb6_f_Rieske.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFTLSSAT PSQLCSSKNG MFAPSLALAK AGRVNVLISK ERIRGMKLTC
60 70 80 90 100
QATSIPADNV PDMQKRETLN LLLLGALSLP TGYMLLPYAS FFVPPGGGAG
110 120 130 140 150
TGGTIAKDAL GNDVIAAEWL KTHAPGDRTL TQGLKGDPTY LVVESDKTLA
160 170 180 190 200
TFGINAVCTH LGCVVPFNAA ENKFICPCHG SQYNNQGRVV RGPAPLSLAL
210 220 230
AHCDVDDGKV VFVPWTETDF RTGEAPWWSA
Length:230
Mass (Da):24,325
Last modified:June 21, 2005 - v2
Checksum:iFA2C54B4C6586059
GO

Sequence cautioni

The sequence CAA29590.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06244 mRNA. Translation: CAA29590.1. Different initiation.
PIRiS00454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06244 mRNA. Translation: CAA29590.1. Different initiation.
PIRiS00454.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RFSX-ray1.83A92-230[»]
ProteinModelPortaliP08980.
SMRiP08980. Positions 59-230.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP08980.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
HAMAPiMF_01335. Cytb6_f_Rieske.
InterProiIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete amino-acid sequence of the Rieske FeS-precursor protein from spinach chloroplasts deduced from cDNA analysis."
    Steppuhn J., Rother C., Hermans J., Jansen T., Salnikow J., Hauska G., Herrmann R.G.
    Mol. Gen. Genet. 210:171-177(1987)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Monatol.
  2. "N-terminal amino acid sequence of the Rieske iron-sulfur protein from the cytochrome b6/f-complex of spinach thylakoids."
    Pfefferkorn B., Meyer H.E.
    FEBS Lett. 206:233-237(1986)
    Cited for: PROTEIN SEQUENCE OF 52-147.
  3. "Biological identity and diversity in photosynthesis and respiration: structure of the lumen-side domain of the chloroplast Rieske protein."
    Carrell C.J., Zhang H., Cramer W.A., Smith J.L.
    Structure 5:1613-1625(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF 92-230 IN COMPLEX WITH 2FE-2S CLUSTER, COFACTOR.

Entry informationi

Entry nameiUCRIA_SPIOL
AccessioniPrimary (citable) accession number: P08980
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: June 21, 2005
Last modified: July 6, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.
The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.