P08968 (RPC1_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: DNA-directed RNA polymerase III subunit RPC1 Short name=RNA polymerase III subunit C1 EC=2.7.7.6 Alternative name(s): DNA-directed RNA polymerase III largest subunit RNA polymerase III subunit C160
Organism	Trypanosoma brucei brucei
Taxonomic identifier	5702 [NCBI]
Taxonomic lineage	Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma

Protein attributes

Sequence length	1530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1530	1530	DNA-directed RNA polymerase III subunit RPC1		PRO_0000073950
Regions
	Region	846 – 858	13	Bridging helix By similarity
Sites
	Metal binding	74	1	Zinc 1 By similarity
	Metal binding	77	1	Zinc 1 By similarity
	Metal binding	84	1	Zinc 1 By similarity
	Metal binding	87	1	Zinc 1 By similarity
	Metal binding	114	1	Zinc 2 By similarity
	Metal binding	117	1	Zinc 2 By similarity
	Metal binding	161	1	Zinc 2 By similarity
	Metal binding	503	1	Magnesium; catalytic By similarity
	Metal binding	505	1	Magnesium; catalytic By similarity
	Metal binding	507	1	Magnesium; catalytic By similarity
Experimental info
	Sequence conflict	1325	1	D → E in AAA30233. Ref.2
	Sequence conflict	1493	1	I → V in AAA30233. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08968 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: FC03D700CEF9D742	FASTA	1,530	170,272
	10 20 30 40 50 60 MLKGSSSTSF LLPQQFVEPL PHAPVEISAL HYGLLSRNDV HRLSVLPCRR VVGDVKEYGV 70 80 90 100 110 120 NDARLGVCDR LSICETCGLN SIECVGHPGH IDLEAPVFHL GFFTTVLRIC RTICKRCSHV 130 140 150 160 170 180 LLDDTEIDYY KRRLSSSSLE PLQRTMLIKT IQTDAYKTRV CLKCGGLNGV VRRVRPMRLV 190 200 210 220 230 240 HEKYHVEPRR GEGPRENPGG FFDAELRTAC AYNKVVGECR EFVHDFLDPV RVRQLFLAVP 250 260 270 280 290 300 PGEVILLGLA PGVSPTDLLM TTLLVPPVPV RPRGCAGTTT VRDDDLTAQY NDILVSTDTM 310 320 330 340 350 360 QDGSLDATRY TETWEMLQMR AARLLDSSLP GFPPNVRTSD LKSYAQRLKS KHGRFRCNLS 370 380 390 400 410 420 GKRVDYSGRS VISPDPNLDV DELAVPLHVA RVLTYPQRVF KANHELMRRL VRNGPHVHPG 430 440 450 460 470 480 ATTVYLAQEG SKKSLKNERD RHRLAARLAV GDIVERHVMN GDLVLFNRQP SLHRVSMMAH 490 500 510 520 530 540 RARVLPFRTF RFNECCCAPY NADFDGDEMN VHFVQTEKAR AEALQLMSTA RNIISAKNGE 550 560 570 580 590 600 PIIACTQDFL AAAYLVTSRD VFFDRGEFSQ MVSHWLGPVT QFRLPIPAIL KPVELWTGKQ 610 620 630 640 650 660 LFELIVRPSP EVDVLLSFEA PTKFYTRKGK HDCAEEGYVA FLDSCFISGR LDKKLLGGGA 670 680 690 700 710 720 KDGLFARLHT IAGGGYTARV MSRIAQFTSR YLTNYGFSLG LGDVAPTPEL NKQKAAVLAR 730 740 750 760 770 780 SVEVCDGLIK SAKTGRMIPL PGLTVKQSLE ARLNTELSKV RDECGTAAVQ TLSIHNNTPL 790 800 810 820 830 840 IMVQSGSKGS ALNIAQMMAC VGQQTVSGKR ILDAFQDRSL PHFHRFEEAP AARGFVANSF 850 860 870 880 890 900 YSGLSPTEFF FHTMAGREGL VDTAVKTAET GYIYRRLMKA MENLSVRYDG TVRNTKGDVI 910 920 930 940 950 960 QLRFGEDGLD PQLMEGNSGT PLNLEQEWLS VRAAYARWVV GLLAGSKTAS DGNAIRDNEN 970 980 990 1000 1010 1020 YFNEFISMLP TEGPSFVEAC LNGDQEALKV CEEQESREDA LHNSNGKTND RESRPRTGRL 1030 1040 1050 1060 1070 1080 RRAVLISHLV KVCSRKFKDD IQDFFVKKVR EQQRIRNLLN LPNTSRERTE GGGDNSGPIA 1090 1100 1110 1120 1130 1140 NKRTKKRAPS LKVKDSKEGG RVSELRDLEM LQTELLPLTR GMVTRFIAQC ASKYLRKACE 1150 1160 1170 1180 1190 1200 PGTPCGAIAA QSVGEPSTQM TLRTFHFAGV ASMSITQGVP RLVEVINANR NIATPVVTAP 1210 1220 1230 1240 1250 1260 VLLMEGEENH CEIFRKRARF VKAQIERVLL REVVSEIVEV CSDTEFYLRV HLNMSVITKL 1270 1280 1290 1300 1310 1320 HLPINAITVR QRILAAAGHT MSPLRMLNED CIEVFSLDTL AVYPHFQDAR WVHFSLRRIL 1330 1340 1350 1360 1370 1380 GLLPDVVVGG IGGINRAMIS SNGTEVLAEG AELRAVMNLW GVDSTRVVCN HVAVVERVLG 1390 1400 1410 1420 1430 1440 IEAARRVIVD EIQNILKAYS LSIDVRHVYL LADLMTQRGV VLGITRYGIQ KMNFNVLTMA 1450 1460 1470 1480 1490 1500 SFERTTDHLY NAAATQRVDR DLSVSDSIIV GKPVPLGTTS FDLLLDGSIS NDILPPQRCV 1510 1520 1530 KRGMGPNFHT AKRHHLVPLA AEGVFRLDLF

References

[1]	"Structure and sequence of the gene for the largest subunit of trypanosomal RNA polymerase III." Cornelissen A.W.C.A., Evers R., Koeck J. Nucleic Acids Res. 16:8753-8772(1988) [PubMed: 3174432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 427 / Isolate MITat 1.2A.
[2]	"Molecular characterization of the Trypanosoma brucei RNA polymerase I and III largest subunit genes." Smith J.L., Levin J.R., Agabian N. J. Biol. Chem. 264:18091-18099(1989) [PubMed: 2808366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X12494 Genomic DNA. Translation: CAA31014.1. M27163 Genomic DNA. Translation: AAA30233.1.
PIR	S01393.
3D structure databases
ProteinModelPortal	P08968.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000722. RNA_pol_asu. IPR015700. RNA_pol_III_lsu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
PANTHER	PTHR19376:SF15. RNA_pol3. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPC1_TRYBB

Accession

Primary (citable) accession number: P08968

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	October 19, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)